中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

应用噬菌体法估测水稻叶片组织中白叶枯病菌（Xanthomonas campestris pv. oryzae）数量研究（英文）

章琦1；苗东华2   

  1. 1 中国农业科学院作物育种与栽培研究所；2 国际水稻研究所，菲律宾马尼拉
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1987-11-18 发布日期:1987-11-18

The Bacteriophage Technique for Estimating Xanthomonas campestris pv. oryzae in Leaf Tissues (in English)

Zhang Qi, T W Mew   

  • Received:1900-01-01 Revised:1900-01-01 Online:1987-11-18 Published:1987-11-18

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摘要： 应用噬菌体法估测了水稻叶片中白叶枯病的增殖。试验表明，溶菌斑数同直接皿测稀释菌液存活细菌数之间表现高度相关，辛尼斯接菌后水稻各个叶位不同时期检测到的菌量与其病斑发展趋势一致，认为在目前对白叶枯病菌缺乏选择性培养基的情况下，这一方法是有效和可靠的。

关键词: 噬菌体技术, 叶片, 稻白叶枯病, 数量

Abstract: The bacteriophage technique was tested to enumerate the bacterial multiplication in leaf tissues of rice. In the absence of a selective medium, the method proved to be efficient and reliable to establish the bacterial number in leaves of different positions of Zenith after infection. A good correlation between phage count and viable count (direct isolation) by the dilution plating was also demonstrated.

Key words: bacteriophage technique, Xanthomonas campstris pv. oryzae