水杨酸减轻高温抑制水稻颖花分化的作用机理研究

doi:10.3969/j.issn.1001G7216.2015.06.010

中国水稻科学 ›› 2015, Vol. 29 ›› Issue (6): 637-647.DOI: 10.3969/j.issn.1001G7216.2015.06.010

水杨酸减轻高温抑制水稻颖花分化的作用机理研究

符冠富, 张彩霞, 杨雪芹, 杨永杰, 陈婷婷, 赵霞, 符卫蒙, 奉保华, 章秀福, 陶龙兴^*(), 金千瑜^*()

中国水稻研究所水稻生物学国家重点实验室,杭州310006;

收稿日期:2015-07-28 修回日期:2015-10-05 出版日期:2015-10-25 发布日期:2015-11-10
通讯作者: 陶龙兴,金千瑜
作者简介:
^*通讯录作者：E-mail:jinqy@mail.hz.zj.cn;lxtao@mailhz.zj.cn
基金资助:
基金项目: 国家自然科学基金资助项目(31501264)浙江省自然科学基金资助项目(LQ15C130003)公益性行业(农业)科研专项(201203029)国家水稻产业体系资助项目(CARS-01-27)比尔和梅琳达·盖茨基金资助项目中央级公益性科研院所专项资金资助项目(2014RG004-4)

Action Mechanism by Which SA Alleviates High Temperature-induced Inhibition to Spikelet Differentiation

Guan-fu FU, Cai-xia ZHANG, Xue-qin YANG, Yong-jie YANG, Ting-ting CHEN, Xia ZHAO, Wei-meng FU, Bao-hua FENG, Xiu-fu ZHANG, Long-xing TAO^*(), Qian-y JIN^*()

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

Received:2015-07-28 Revised:2015-10-05 Online:2015-10-25 Published:2015-11-10
Contact: Long-xing TAO, Qian-y JIN
About author:
^*Corresponding author：E-mail:jinqy@mail.hz.zj.cn;lxtao@mailhz.zj.cn

摘要/Abstract

摘要：

水稻颖花原基分化期高温可抑制颖花分化,导致每穗粒数下降,最终会影响水稻产量的形成。以杂交稻甬优12为材料,探讨高温下不同浓度水杨酸(salicylic acid, SA)对水稻颖花分化的影响,并揭示其作用机制。研究表明,常温下适量SA促进水稻颖花分化,但高浓度SA严重降低每穗粒数。然而高温下,10~50000 μmol/L SA处理每穗粒数均高于对照。无论常温或高温,100 μmol/L水杨酸处理效果最好,其每穗粒数及产量均显著高于对照。SA增强水稻耐热性表现在三个方面:1)高温下SA处理的叶片实际荧光量子效率及净光合速率均高于对照;2)高温下SA处理的颖花SOD、POD及CAT活性显著高于对照,而MDA含量则显著低于对照;3)无论高温或常温,SA处理的颖花ZR及IAA含量均高于对照,尤其在高温下,差异达显著水平。

关键词: 水稻, 水杨酸, 高温胁迫, 颖花分化

Abstract:

Heat stress at the spikelet primordium differentiation stage of rice significantly decreases the grain yield due to reduced grain number per panicle. The hybrid rice Yongyou 12 was used as material to investigate the role of salicylic acid(SA) in alleviating the negative effect on spikelet differentiation induced by heat stress. The results indicated that under natural conditions, low concentration of SA increased the spikelet number per panicle, while high concentration decreased the number. Interestingly, either low or high concentrations of SA (10-50000 μmol/L) increased the grain number per panicle under heat stress. The grain yield and grain number per panicle peaked under the treatment of 100 μmol/L SA compared with their respective control (0 μmol/L) under both natural conditions and heat stress. This might attribute to the increase in the leaf photosynthesis rate, antioxidant enzyme activities, concentrations of auxin(IAA) and zeatin(ZR) in spikelets under heat stress. Heat stress occurred at spikelet differentiation stage seemed to cause little damage to the leaf, but the actual light quantum efficiency and net photosynthetic rate of leaf were increased when sprayed with SA. Accordingly, there was no significant difference in the activities of SOD, POD and CAT of leaf in rice. However, these antioxidant enzyme activities in spikelets were significantly decreased when subjected to heat stress, especially under the treatment of 0 μmol/L SA, suggesting that SA could enhance anti-oxidation. Further, SA could also prevent the decrease in the concentrations of IAA and ZR in spikelets induced by heat stress. In sum, SA obviously enhanced heat-resistance in rice at 100-1000 μmol/L.

Key words: rice, salicylic acid, heat stress, spikelet differentiation

符冠富, 张彩霞, 杨雪芹, 杨永杰, 陈婷婷, 赵霞, 符卫蒙, 奉保华, 章秀福, 陶龙兴, 金千瑜. 水杨酸减轻高温抑制水稻颖花分化的作用机理研究[J]. 中国水稻科学, 2015, 29(6): 637-647.

Guan-fu FU, Cai-xia ZHANG, Xue-qin YANG, Yong-jie YANG, Ting-ting CHEN, Xia ZHAO, Wei-meng FU, Bao-hua FENG, Xiu-fu ZHANG, Long-xing TAO, Qian-y JIN. Action Mechanism by Which SA Alleviates High Temperature-induced Inhibition to Spikelet Differentiation[J]. Chinese Journal OF Rice Science, 2015, 29(6): 637-647.

图/表 10

图1 高温下水杨酸对水稻颖花分化的影响(2013)

Fig.1. Effect of SA on the spikelet differentiation of rice under heat stress in 2013.

表1 高温下SA对水稻产量及其构成的影响(2014)

Table 1 Effect of salicylic acid(SA) on grain yield and its components of rice under heat stress(2014).

温度 Temperature	水杨酸浓度 SAC /(μmol·L^-1)	穗数 PNPP	每穗粒数 GNPP	结实率 SSR/%	千粒重 TGW/g	产量GY /(g·pot^-1)
常温Natural temperature
	0	16.0±0.7 abc	429.7±18.3 abcd	87.6±0.8 abcd	21.9±0.1 bcd	131.4±5.9 bc
	10	16.0±0.7 abc	440.2±46.2 ab	88.7±1.1 abc	21.2±0.5 e	132.4±13.0 bc
	100	16.8±0.7 a	450.8±28.5 a	90.9±2.1 a	21.9±0.2 abcd	150.8±12.3 a
	1000	16.8±1.3 a	437.6±40.9 abc	89.3±5.0 ab	21.5±0.1 cde	141.1±12.8 ab
	10000	16.0±0.7 abc	414.1±9.7 abcde	86.0±2.0 bcde	21.5±0.6 de	122.0±9.5 cde
	50000	16.0±1.1 abc	395.7±27.8 def	84.9±3.1 de	21.5±0.5 de	115.3±6.1 def
高温High temperature
	0	15.5±1.2 abcd	364.6±29.0 f	83.6±2.5 e	22.4±0.3 a	106.0±11.2 fg
	10	15.0±1.2 bcd	408.3±15.5 bcde	85.1±1.3 de	22.2±0.3 ab	115.4±10.8 def
	100	15.5±0.7 abcd	434.4±27.2 abc	83.6±2.1 e	22.5±0.7 a	126.3±4.6 cd
	1000	16.0±1.3 ab	401.7±17.5 cde	82.9±1.8 e	21.7±0.6 bcde	115.5±8.6 def
	10000	14.7±1.5 cd	405.7±39.0 bcde	85.8±1.6 cde	21.9±0.5 bcd	111.2±7.0 efg
	50000	14.4±1.7 cd	375.9±40.9 ef	84.8±4.6 de	22.2±0.7 abc	101.8±20.6 g

图2 高温下SA对水稻叶片叶绿素含量的影响(2014)

Fig. 2. Effect of salicylic acid(SA) on chlorophyll contents of rice leaf under heat stress in 2014.

图3 高温下SA对水稻叶片叶绿素荧光参数的影响(2014)

Fig. 3. Effect of salicylic acid(SA) on chlorophyll-fluorescence parameters of rice leaf under heat stress in 2014.

图4 高温下SA对水稻叶片净光合速率及蒸腾速率的影响(2014)

Fig. 4. Effect of salicylic acid(SA) on net photosynthetic rate and transpiration rate of leaf in rice under heat stress in 2014.

图5 高温下SA对水稻叶片温度的影响(2014)

Fig. 5. Effect of salicylic acid(SA) on leaf temperature in rice under heat stress in 2014.

图6 高温下水杨酸对水稻叶片及颖花抗氧化酶活性的影响(2014)

Fig. 6. Effect of salicylic acid(SA) on antioxidant enzyme activities in leaf and spikelet in rice under heat stress in 2014.

图7 高温下SA对水稻叶片及颖花丙二醛(MDA)含量的影响(2014)

Fig. 7. Effect of SA on the MDA content of leaf and spikelet in rice under heat stress in 2014.

图8 高温下SA对水稻颖花植物激素含量的影响(2014)

Fig. 8. Effect of SA on the phytohormone concentrations of spikelet in rice under heat stress in 2014.

图9 高温下SA对水稻叶片及颖花可溶性糖含量的影响(2014)

Fig. 9. Effect of SA on the soluble sugar contents of leaf and spikelet in rice under heat stress in 2014.

参考文献 41

[1]	Kaoru K,Masahiko M,Akio M,et al.Encoding a SEPALLATA subfamily MADS-box protein,positively controls spikelet meristem identity in rice.Plant Cell Physiol, 2010,51(1):47-57.
[2]	Keishi K,Masahiko M,Shin U,et al.LAX and SPA: Major regulators of shoot branching in rice.Proc Natl Acad Sci USA, 2003,100(20):11765-11770.
[3]	Li S,Qian Q,Fu Z,et al.Short panicle 1 encodes a putative PTR family transporter and determines rice panicle size.Plant J, 2009,58,592-605.
[4]	Kurakawa T,Ueda N,Maekawa M,et al.Direct control of shoot meristem activity by a cytokinin-activating enzyme.Nature, 2007,445(7128):652-655.
[5]	Veronica O,Ottoline L.Hormonal control of shoot branching.J Exp Bot, 2008,59(1):67-74.
[6]	Ottoline L.Regulation of shoot branching by auxin.Trends Plant Sci, 2003,8(11):541-545.
[7]	Shimizu-Sato S,Mina T,Hitoshi M.Auxin-cytokinin interactions in the control of shoot branching.Plant Mol Biol,2009,69:429-435.
[8]	杨军,陈小荣,朱昌兰,等. 氮肥和孕穗后期高温对两个早稻品种产量和生理特性的影响.中国水稻科学,2014,28(5):523-533.
[9]	Yao Y L,Yoshinori Y,Tetsushi Y,et al.Response of differentiated and degenerated spikelets to top-dressing,shading and day/night temperature treatments in rice cultivars with large panicles.Soil Sci Plant Nutr, 2000,46(3):631-641.
[10]	Shah F,Huang J L,Cui K H,et al.Impact of high-temperature stress on rice plant and its traits related to tolerance.J Agr Sci, 2011,149:545-556.
[11]	Mohammed A R,Tarpley L.High night temperature and plant growth regulator effects on spikelet sterility,grain characteristics and yield of rice (Oryza sativa L.) plants.Can J Plant Sci, 2011,91: 283-291.
[12]	Senaratna T,Toucbell D,Bunn E,et al.Acetylsalicylic acid and salicylic acid induce multiple stress tolerance in bean and tomato plants.Plant Growth Regul, 2000,30:157-161.
[13]	Harper J R,Balke N E.Characterization of the inhibition of k absorption in oat roots by salicylic Acid.Plant Physiol, 1981,68(6):1349-1353.
[14]	Khan W,Prithiviraj B,Smith D L.Photosynthetic responses of corn and soybean to foliar application of salicylates.J Plant Physiol, 2003,160(5):485-492.
[15]	Shi Q H,Bao Z Y,Zhu Z J,et al.Effects of Different treatments of salicylic acid on heat tolerance,chlorophyll fluorescence,and antioxidant enzyme activity in seedlings of cucumis sativa L.Plant Growth Regul, 2006,48(2):127-135.
[16]	王小玲,高柱,余发新,等.外源水杨酸对观赏羽扇豆高温胁迫的生理响应.中国农学通报,2011,27(25):89-93.
[17]	Zhao H J,Zhao X J,Ma P F,et al.Effects of salicylic acid on protein kinase activity and chloroplast D1 protein degradation in wheat leaves subjected to heat and high light stress.Acta Ecol Sin, 2011,31(5): 259-263.
[18]	Giannopolitis C N,Ries S K.Superoxide dismutase.1.Occurrence in higher plant.Plant Physiol, 1977,59:309-314.
[19]	Maehly A C,Chance B.The assay of catalases and peroxidases.Meth Biochem Anal,1954,1(3):357-424.
[20]	Aebi H E.Catalase//Bergmeyer H U. ed. Methods of Enzymatic Analysis. Verlarg Chemie, Berlin, 1983: 278-282.
[21]	赵世杰,史国安,董新纯.植物生理学实验指导. 泰安:中国农业科学技术出版社,2002:141.
[22]	Dhindsa R S,Plumb-dhindsa P, Thorpe T A.Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation,and decreased levels of superoxide dismutase and catalase.J Exp Bot, 1981,32(1):93-101.
[23]	DuBois M,Gilles K A,Hamilton J K,et al. Colorimetric method for determination of sugars and related substances.Anal Chem, 1956,28(3):350-356.
[24]	Lopez-delgado H, Scott I M.Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2.J Exp Bot, 1998,49(321):713-720.
[25]	Tang R S,Zheng J C,Jin Z Q,et al.Possible correlation between high temperature-induced floret sterility and endogenous levels of IAA,GAs and ABA in rice (Oryza sativa L.).Plant Growth Regul, 2008,54:37-43.
[26]	Sharkey T D.Effects of moderate heat stress on photosynthesis: Importance of thylakoid reactions,rubisco deactivation,reactive oxygen species,and thermotolerance provided by isoprene.Plant Cell Environ, 2005,28:269-277.
[27]	Zhang C X, Fu G F, Yang X Q,et al.Heat stress effects are stronger on spikelets than on flag leaves in rice due to differences in dissipation capacity.J Agron Crop Sci,2015,doi: 10.1111/jac.12138.
[28]	Rahat N,Noushina I,Shabina S,et al.Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mungbean cultivars.J Plant Physiol, 2011,168:807-815.
[29]	Shamsul H,Qaiser H,Mohammed N A,et al.Salicylic acid enhances the efficiency of nitrogen fixation and assimilation in Cicer arietinum plants grown under cadmium stress.J Plant Interact, 2014,9(1):35-42.
[30]	Yang Q M,Mei C.Endogenous salicylic acid protects rice plants from oxidative damage caused by aging as well as biotic and abiotic stress.Plant J, 2004,40:909-919.
[31]	徐芬芬,叶利民,付淑琴.外源水杨酸对水稻幼苗抗冷性的影响.广东农业科学,2010,37(1):18-20.
[32]	Rivas-San Vicente M, Plasencia J.Salicylic acid beyond defence: Its role in plant growth and development.J Exp Bot, 2011,62(10): 3321-3338.
[33]	Oikawa T,Kyozuka J.Two-step regulation of LAX PANICLE1 protein accumulation in axillary meristem formation in rice.Plant Cell, 2009,21(4):1095-1108.
[34]	Ikeda K,Ito M,Nagasawa N,et al.Rice ABERRANT PANICLE ORGANIZATION 1,encoding an F-box protein,regulates meristem fate.Plant J, 2007,51(6):1030-1040.
[35]	Levin J Z,Meyerowitz E M.UFO:An Arabidopsis gene involved in both floral meristem and floral organ development.Plant Cell, 1995,7(5):529-548.
[36]	Jiang C J,Masaki S,Shoji S,et al.Cytokinins act synergistically with salicylic acid to activate defense gene expression in rice.Mol Plant Microbe Interact, 2013,26(3):287-296.
[37]	Kang H G,Singh K B.Characterization of salicylic acidresponsive Arabidopsis Dof domain proteins: Overexpression of OBP3 leads to growth defects.Plant J, 2000,21:329-339.
[38]	Shakirova F M,Sakhabutdinova A R,Bezrukova M V,et al.Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity.Plant Sci, 2003,164:317-322.
[39]	Rajjou L,Belghazi M,Huguet R,et al.Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms.Plant Physiol, 2006,141:910-923.
[40]	Xie Z,Zhang Z L,Hanzlik S,et al.Salicylic acid inhibits gibberellin-induced alpha-amylase expression and seed germination via a pathway involving an abscisic-acid inducible WRKY gene.Plant Mol Biol, 2007,64:293-303.
[41]	Guan L,Scandalios J G.Developmentally related responses of maize catalase genes to salicylic acid.Proc Natl Acad Sci USA, 1995,92:5930-5934.

水杨酸减轻高温抑制水稻颖花分化的作用机理研究

Action Mechanism by Which SA Alleviates High Temperature-induced Inhibition to Spikelet Differentiation

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