水稻窄卷叶突变体Nrl3(t)的基因定位

doi:10.3969/j.issn.1001G7216.2015.06.005

中国水稻科学 ›› 2015, Vol. 29 ›› Issue (6): 595-600.DOI: 10.3969/j.issn.1001G7216.2015.06.005

水稻窄卷叶突变体Nrl3(t)的基因定位

张晓惠^1,^2,³, 秦亚芝^1,², 张迎信², 占小登², 张振华², 沈希宏², 程式华², 曹立勇^2,^*(), 吴先军^1,^*()

收稿日期:2015-02-09 修回日期:2015-09-06 出版日期:2015-10-25 发布日期:2015-11-10
通讯作者: 曹立勇,吴先军
作者简介:
^*通讯录作者：E-mail:wuxjsau@126.com,caolycgf@mail.hz.zj.cn
基金资助:
中央级公益性科研院所基本科研业务费专项(2014RG002-7)中国农业科学院水稻杂种优势机理研究创新团队项目(CAAS-ASTIP-2013-CNRRI)

Gene Mapping of a Narrow and Rolled Leaf Mutant Nrl3(t) in Rice

Xiao-hui ZHANG^1,^2,³, Ya-zhi QIN^1,², Ying-xin ZHANG², Xiao-deng ZHAN², Zhen-hua ZHANG², Xi-hong SHEN², Shi-hua CHENG², Li-yong CAO²(), Xian-jun WU^1,^*()

¹Rice Research Institute/Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu 611130, ChinaNational Center for Rice Improvement,China National Rice Research Institute, Hangzhou, 310006, China
²National Center for Rice Improvement,China National Rice Research Institute, Hangzhou, 310006, China
³Zhejiang National Rice High-Tech Plants Industry Limited Company, Hangzhou 311401, China

Received:2015-02-09 Revised:2015-09-06 Online:2015-10-25 Published:2015-11-10
Contact: Xian-jun WU
About author:
^*Corresponding author：E-mail:wuxjsau@126.com,caolycgf@mail.hz.zj.cn

摘要/Abstract

摘要：

利用⁶⁰Co-γ射线辐射诱变籼稻恢复系中恢8015,获得了一份窄卷叶突变体Nrl3(t)。与野生型中恢8015相比,突变体叶片显著变窄内卷、株高降低、穗长变短、结实率降低、千粒重降低、粒长粒宽减小。细胞学分析表明,突变体维管束减少和泡状细胞不够饱满是导致叶片变窄卷曲的原因。遗传分析表明该突变体受一对隐性核基因控制,以窄卷叶突变体Nrl3(t)与粳稻品种02428杂交获得的F₂分离群体作为定位群体,利用SSR标记和新开发的InDel标记,将窄卷叶基因Nrl3(t)定位于第2染色体长臂标记C9和C10E之间约70.3 kb区间内。研究结果为该基因的克隆及进一步揭示水稻窄卷叶形成的分子机理奠定了基础。

关键词: 水稻, 窄卷叶, 基因, 精细定位

Abstract:

Nrl3(t) was a novel narrow and rolled leaf mutant from the indica restorer line Zhonghui 8015 after exposure to ⁶⁰Co-γ ray. Nrl3(t) exhibited significant adaxially-rolled leaves, reduced plant height, shortened panicle length, reduced grain length, grain width and thousand-grain weight, as well as the seed-setting rate. Cytological analysis showed that the rolled leaf phenotype was due to the reduced number of vascular bundleand the changed size of bulliform cells. In an initial mapping with F₂ individuals derived from a cross between Nrl3(t) and japonica 02428, the narrow and rolled leaf gene Nrl3(t) was eventually mapped to a 70.3 kb region on the long arm of chromosome 2 between two newly developed sequence tagged site markers C9 and C10E. These results would lay a good foundation for molecular cloning and functional analysis of Nrl3(t).

Key words: rice, narrow and rolled leaf, gene, fine mapping

中图分类号:

张晓惠, 秦亚芝, 张迎信, 占小登, 张振华, 沈希宏, 程式华, 曹立勇, 吴先军. 水稻窄卷叶突变体Nrl3(t)的基因定位[J]. 中国水稻科学, 2015, 29(6): 595-600.

Xiao-hui ZHANG, Ya-zhi QIN, Ying-xin ZHANG, Xiao-deng ZHAN, Zhen-hua ZHANG, Xi-hong SHEN, Shi-hua CHENG, Li-yong CAO, Xian-jun WU. Gene Mapping of a Narrow and Rolled Leaf Mutant Nrl3(t) in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(6): 595-600.

图/表 7

参考文献 24

[1]	陈温福, 徐正进, 张龙步.水稻理想株型的研究.沈阳农业大学学报, 1989, 20(4):417-420.
[2]	袁隆平. 杂交水稻超高产育种.杂交水稻, 1997, 12(6):1-3.
[3]	Xiang J J, Zhang G H, Qian Q, et al.SEMI-ROLLED LEAF1 encodes a putative glycosylphosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells.Plant Physiol, 2012, 159(4): 1488-1500.
[4]	Li L, Shi Z Y, Li L, et al.Overexpression of ACL1 (abaxially curled leaf 1) increased bulliform cells and induced abaxial curling of leaf blades in rice.Mol Plant, 2010, 3(5): 807-817.
[5]	Zou L P, Sun X H, Zhang Z G, et al.Leaf rolling controlled by the homeodomain leucine zipper class IV gene Roc5 in rice.Plant Physiol, 2011, 156(3): 1589-1602.
[6]	Hibara K,Obara M,Hayashida E, et al.The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation in rice.Devel Biol,2009,334(2):345-354.
[7]	Fang L K, Zhao F M, Cong Y F, et al.Rolling-leaf14 is a 2OG-Fe (II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves.Plant Biotechnol J, 2012, 10(5): 524-532.
[8]	Li M, Xiong G Y, Li R, et al.Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth.Plant J, 2009, 60(6): 1055-1069.
[9]	Wu C, Fu Y P, Hu G H, et al.Isolation and characterization of a rice mutant with narrow and rolled leaves.Planta, 2010, 232(2): 313-324.
[10]	朱丽, 胡江, 颜美仙, 等. 水稻卷窄叶突变相关基因OsCSLD4 的RNAi研究及表达分析. 核农学报, 2010, 24(5): 873-880.
[11]	Hu J, Zhu L, Zeng D L, et al.Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice.Plant Mol Biol, 2010, 73(3): 283-292.
[12]	Yoshikawa T, Eiguchi M, Hibara K I, et al.Rice SLENDER LEAF 1 gene encodes cellulose synthase-like D4 and is specifically expressed in M-phase cells to regulate cell proliferation.J Exper Bot, 2013, 64(7): 2049-2061.
[13]	Yang C H, Li D Y, Liu X, et al.OsMYB103L, an R2R3-MYB transcription factor, influences leaf rolling and mechanical strength in rice (Oryza sativa L.).BMC Plant Biol, 2014, 14: 158.
[14]	Luan W J, Liu Y Q, Zhang F G, et al.OsCD1 encodes a putative member of the cellulose synthase-like D sub-family and is essential for rice plant architecture and growth.Plant Biotechnol J, 2011, 9(4): 513-524.
[15]	Shi Z Y, Wang J, Wan X S, et al.Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit.Planta, 2007, 226(1): 99-108.
[16]	Zhang G H, Xu Q, Zhu X D, et al.SHALLOT-LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development.Plant Cell, 2009, 21(3): 719-735.
[17]	Wu R H, Li S B, He S, et al.CFL1, a WW domain protein, regulates cuticle development by modulating the function of HDG1, a class IV homeodomain transcription factor, in rice and Arabidopsis.Plant Cell, 2011, 23(9): 3392-3411.
[18]	Fujino K,Matsuda Y, Ozawa K, et al.NARROW LEAF 7 controls leaf shape mediated by auxin in rice.Mol Genet Genom, 2008, 279(5): 499-507.
[19]	Cho S H, Yoo S C, Zhang H T, et al.The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development.New Phytol, 2013, 198(4): 1071-1084.
[20]	Qi J, Qian Q, Bu Q Y, et al.Mutation of the rice narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport.Plant Physiol, 2008, 147(4): 1947-1959.
[21]	Chen M L,Luo J, Shao G N, et al.Fine mapping of a major QTL for flag leaf width in rice, qFLW4, which might be caused by alternative splicing of NAL1.Plant Cell Rep, 2011, 31(5): 863-872.
[22]	Zhang G H, Li S Y, Wang L, et al.LSCHL4 from Japonica cultivar,which is allelic to NAL1, increases yield of Indica super rice 93-11.Mol Plant, 2014,7(8): 1350-1364.
[23]	卢扬江,郑康乐. 提取水稻DNA的一种简易方法. 中国水稻科学,1992,6(1): 47-48.
[24]	Orjuela J,Garavito A,Bouniol M,et al.A universal core genetic map for rice.Theor Appl Genet, 2010,120(3): 563-572.

性状 Trait	Zh8015	Nrl3(t)
株高 Plant height/cm	108.13±3.14	72.08±2.47^**
有效穗Effective panicle number	7.45±1.76	11.53±3.22^**
穗长Panicle length/cm	25.29±1.18	15.91±1.22^**
穗粒数Grain number per panicle	134.65±18.17	57.13±5.55^**
结实率Seed-setting rate/%	90.08±0.03	66.19±0.06^**
千粒重1000-grain weight/g	32.79±1.83	25.51±1.63^**
粒宽Seed width/mm	3.21±0.04	2.85±0.04^**
粒长Seed length/mm	10.01±0.07	8.73±0.05^**

性状 Trait	Zh8015	Nrl3(t)
株高 Plant height/cm	108.13±3.14	72.08±2.47^**
有效穗Effective panicle number	7.45±1.76	11.53±3.22^**
穗长Panicle length/cm	25.29±1.18	15.91±1.22^**
穗粒数Grain number per panicle	134.65±18.17	57.13±5.55^**
结实率Seed-setting rate/%	90.08±0.03	66.19±0.06^**
千粒重1000-grain weight/g	32.79±1.83	25.51±1.63^**
粒宽Seed width/mm	3.21±0.04	2.85±0.04^**
粒长Seed length/mm	10.01±0.07	8.73±0.05^**

标记 Marker	正向序列(5'-3') Forward seque nce (5'-3')	反向序列(5'-3') Reverse sequence (5'-3')	位置 Location
4-8	GCAATGCAGGTGGTTTAG	CTTGATGCCGGTGTTGGC	32569845
C8E	GACGGCGACTCCGAACC	GAGAGGGGGCAAAAGGTGAA	32753982
C9	CGGTTCACACGAATTGACCC	AGATGATTGAAAAACACGGGAAT	32793884
C10E	CTCCAACCTGAGGCATCCAC	CGCTATTTGCCGAGGAGGAA	32864280
C12	TGCCTATACTGATTCGCCGC	GAGTCAGCCCGTGACTTGTA	32970608
C15	CGCCAACTTACAAGCGATGT	CTTGCCGTCTCTGCTGCAT	33094078
N26	GGTGGCCGTACTGATACTCC	AGCTGCCTACTTTACCTAGCTG	33682812

标记 Marker	正向序列(5'-3') Forward seque nce (5'-3')	反向序列(5'-3') Reverse sequence (5'-3')	位置 Location
4-8	GCAATGCAGGTGGTTTAG	CTTGATGCCGGTGTTGGC	32569845
C8E	GACGGCGACTCCGAACC	GAGAGGGGGCAAAAGGTGAA	32753982
C9	CGGTTCACACGAATTGACCC	AGATGATTGAAAAACACGGGAAT	32793884
C10E	CTCCAACCTGAGGCATCCAC	CGCTATTTGCCGAGGAGGAA	32864280
C12	TGCCTATACTGATTCGCCGC	GAGTCAGCCCGTGACTTGTA	32970608
C15	CGCCAACTTACAAGCGATGT	CTTGCCGTCTCTGCTGCAT	33094078
N26	GGTGGCCGTACTGATACTCC	AGCTGCCTACTTTACCTAGCTG	33682812

水稻窄卷叶突变体Nrl3(t)的基因定位

Gene Mapping of a Narrow and Rolled Leaf Mutant Nrl3(t) in Rice

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