水稻灌浆过程中胚乳异淀粉酶基因家族表达特性及其与淀粉含量间关系分析

doi:10.3969/j.issn.1001G7216.2015.05.010

摘要/Abstract

摘要：

选用胚乳直链淀粉和支链淀粉含量不同的5个粳稻品种,分析籽粒灌浆过程中异淀粉酶基因在胚乳中的表达特性和酶活性变化及其与淀粉含量间的关系。结果表明,在灌浆过程中胚乳异淀粉酶活性及ISA1和ISA3基因的mRNA表达量,直链淀粉含量不同的非糯品种都呈先升后降的单峰曲线变化。灌浆中期出现峰值,但糯稻品种呈直线上升的变化趋势,后期的表达量明显高于前期;ISA2基因mRNA表达量自抽穗后10 d起随灌浆进程逐渐下降;在灌浆过程中胚乳ISAs基因的mRNA表达量与异淀粉酶活性正相关;ISA1和ISA3基因mRNA表达量、异淀粉酶活性及支链淀粉含量呈同步变化。在整个灌浆过程中ISA1基因和ISA1和ISA3基因的表达量明显大于ISA2基因,是灌浆过程中主要表达的基因。

关键词: 水稻, 灌浆, 异淀粉酶, 基因表达, 淀粉含量, 相关分析

Abstract:

By using five japonica rice varieties with different amylose and amylopectin contents in endosperm as experimental material, we analyzed the expression levels of ISAs and the activity of isomylase, and their correlation with starch content during grain filling. It turned out that the isomylase activity, the expression levels of ISA1 and ISA3 of tested varieties followed a unimodal curve with peak value appearing during the mid-filling stage except sticky rice, while for the sticky rice, the expression level showed a straight-line growth during grain filling with obvious higher expression in late stage. The expression level of ISA2 was down-regulated ten days after heading. In principle, the expression levels of ISA1 and ISA3 were significantly positively correlated with the enzyme activity, and they both synchronously changed with the content of amylopectin. During grain filling, the expression levels of ISA1 and ISA3 were absolutely higher than that of ISA2, indicating that they were dominated genes expressed during grain filling.

Key words: japonica rice, grain filling, isoamylase, gene expression, starch content, correlation analysis

中图分类号:

朱立楠, 刘海英, 孙璐璐, 孙涛, 郭雪冬, 朱方旭, 张忠臣, 金正勋. 水稻灌浆过程中胚乳异淀粉酶基因家族表达特性及其与淀粉含量间关系分析[J]. 中国水稻科学, 2015, 29(5): 528-534.

Li-nan ZHU, Hai-ying LIU, Lu-lu SUN, Tao SUN, Xue-dong GUO, Fang-xu ZHU, Zhong-chen ZHANG, Zheng-xun JIN. Analysis of Expression Characteristics of Isoamylase and the Correlation with Starch Content During Grain Filling in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(5): 528-534.

图/表 8

参考文献 21

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基因名称 Gene name	登录号 Accession No.	正向引物(5'-3') Forward primer(5'-3')	反向引物(5'-3') Reverse primer(5'-3')	产物片段 Production size/bp
ISA1	NM_001068806.1	GTGGCCTTCTCCACGAAAGA	ATGAGAGAACAGGTGGACGG	201
ISA2	NM_001061991.2	GGGGACCTGTTCTCAACCAG	CATCCCAACCTGGCTCACAT	446
ISA3	NM_001069968.1	CCTTTATCTCGTAGGGCGTTTC	CGTGCCAGGTGACATCGTT	617
Actin	NM_001057621.1	GGAGCGTGGTTACTCATTC	CTCTTGGCTTAGCATTCTTG	578

基因名称 Gene name	登录号 Accession No.	正向引物(5'-3') Forward primer(5'-3')	反向引物(5'-3') Reverse primer(5'-3')	产物片段 Production size/bp
ISA1	NM_001068806.1	GTGGCCTTCTCCACGAAAGA	ATGAGAGAACAGGTGGACGG	201
ISA2	NM_001061991.2	GGGGACCTGTTCTCAACCAG	CATCCCAACCTGGCTCACAT	446
ISA3	NM_001069968.1	CCTTTATCTCGTAGGGCGTTTC	CGTGCCAGGTGACATCGTT	617
Actin	NM_001057621.1	GGAGCGTGGTTACTCATTC	CTCTTGGCTTAGCATTCTTG	578

品种 Variety	抽穗后天数 Days after heading/d					精米 Milled rice
品种 Variety	10		15	20	25	30
东农423 Dongnong 423	9.38 a	13.35 a	15.63 a	17.29 a	18.65 a	18.82 a
系选1号 Xixuan 1	9.16 b	12.92 b	15.18 b	16.86 b	18.47 b	18.74 b
藤系180 Toukei 180	4.59 c	6.84 c	9.17 c	10.18 c	11.02 c	11.05 c
东农1024 Dongnong 1024	2.14 d	3.62 d	5.23 d	6.13 d	7.33 d	7.41 d
龙稻8号 Longdao 8	0.73 e	0.89 e	0.95 e	1.05 e	1.11 e	1.26 e

品种 Variety	抽穗后天数 Days after heading/d					精米 Milled rice
品种 Variety	10		15	20	25	30
东农423 Dongnong 423	9.38 a	13.35 a	15.63 a	17.29 a	18.65 a	18.82 a
系选1号 Xixuan 1	9.16 b	12.92 b	15.18 b	16.86 b	18.47 b	18.74 b
藤系180 Toukei 180	4.59 c	6.84 c	9.17 c	10.18 c	11.02 c	11.05 c
东农1024 Dongnong 1024	2.14 d	3.62 d	5.23 d	6.13 d	7.33 d	7.41 d
龙稻8号 Longdao 8	0.73 e	0.89 e	0.95 e	1.05 e	1.11 e	1.26 e

品种 Variety	抽穗后天数 Days after heading/d					精米 Milled rice
品种 Variety	10		15	20	25	30
东农423 Dongnong 423	47.51 b	53.42 b	57.34 b	59.95 d	61.96 c	62.11 c
系选1号 Xixuan 1	44.44 c	52.20 b	54.13 b	55.83 e	55.13 d	55.15 d
藤系180 Toukei 180	42.73 e	55.42 b	57.68 b	61.80 c	62.07 c	62.11 c
东农1024 Dongnong 1024	43.25 d	54.52 b	59.11 b	64.81 b	65.68 b	65.88 b
龙稻8号 Longdao 8	57.78 a	69.08 a	77.22 a	80.03 a	81.83 a	82.09 a