中国水稻科学 ›› 2018, Vol. 32 ›› Issue (5): 475-484.DOI: 10.16819/j.1001-7216.2018.8013
孙涛, 同拉嘎, 赵书宇, 王海微, 韩云飞, 张忠臣, 金正勋*()
收稿日期:
2018-01-29
修回日期:
2018-04-23
出版日期:
2018-09-10
发布日期:
2018-09-10
通讯作者:
金正勋
基金资助:
Tao SUN, Laga TONG, Shuyu ZHAO, Haiwei WANG, Yunfei HAN, Zhongchen ZHANG, Zhengxun JIN*()
Received:
2018-01-29
Revised:
2018-04-23
Online:
2018-09-10
Published:
2018-09-10
Contact:
Zhengxun JIN
摘要:
【目的】旨在为阐明灌浆成熟期氮素营养对水稻淀粉品质影响机理以及建立优质高产水稻栽培技术提供理论依据。【方法】选用4个籽粒直链淀粉含量差异显著的粳稻品种,通过盆栽试验研究氮素营养对稻米淀粉组分和蛋白质含量及稻米蛋白质水解对淀粉黏滞特性的影响,并分析氮素营养对灌浆过程中籽粒蔗糖合酶(SuSy)、蔗糖磷酸合酶(SPS)、蔗糖酸性转化酶(AI)活性及OsGBSSⅠ、OsISAⅠ、OsSBEⅠ、OsSBEⅡ基因转录表达量的影响。【结果】结果表明,籽粒支链淀粉含量对氮素营养很敏感,灌浆成熟期氮素营养能改变籽粒淀粉组分含量;施氮条件下,高直链淀粉含量品种蒸煮食味品质下降更加明显,去除稻米蛋白质可明显提高稻米黏滞特性,蛋白质对淀粉黏滞特性的影响很大;增加灌浆成熟期氮素营养能显著或极显著提高籽粒SuSy和AI活性,显著抑制籽粒SPS活性;灌浆成熟期氮素营养能改变灌浆不同时期籽粒OsGBSSⅠ、OsISAⅠ和OsSBEⅡ基因转录表达量,以致灌浆过程中这些基因的转录表达量变化动态发生改变,但OsSBEⅠ基因转录表达量不因氮素营养而发生改变;受氮素营养的影响,灌浆起始期籽粒OsGBSSⅠ基因表达量明显上调,而灌浆中后期明显下调;氮素营养明显抑制灌浆成熟期籽粒OsISAⅠ基因和灌浆中后期的OsSBEⅠ基因转录表达,显著提高灌浆前期和中期的籽粒OsSBEⅠ基因转录表达量;氮素营养能抑制灌浆起始期籽粒OsSBEⅡ基因的转录表达,而提高灌浆中后期的基因转录表达。【结论】灌浆成熟期氮素营养除了通过蛋白质含量对淀粉品质产生影响外,还通过调控淀粉合成相关的酶活性和基因表达量等生理环节对淀粉含量和精细结构起作用,最终改变稻米黏滞特性和食味品质。
中图分类号:
孙涛, 同拉嘎, 赵书宇, 王海微, 韩云飞, 张忠臣, 金正勋. 氮肥对水稻胚乳淀粉品质、相关酶活性及基因表达量的影响[J]. 中国水稻科学, 2018, 32(5): 475-484.
Tao SUN, Laga TONG, Shuyu ZHAO, Haiwei WANG, Yunfei HAN, Zhongchen ZHANG, Zhengxun JIN. Effects of Nitrogen Fertilizer Application on Starch Quality, Activities and Gene Expression Levels of Related Enzymes in Rice Endosperm[J]. Chinese Journal OF Rice Science, 2018, 32(5): 475-484.
基因名称 Gene name | 登录号 Accession number | 上游引物(5′-3′) Forward primer(5′-3′) | 下游引物(5′-3′) Reverse primer(5′-3′) | 产物片段 Production size/bp |
---|---|---|---|---|
OsGBSSⅠ | XM_015789006.1 | AGCGTTGTGGCTGAGATCAA | CTTCTCACCGGTCTTTCCCC | 138 |
OsISAⅠ | XM_015794756.1 | GTGGCCTTCTCCACGAAAGA | CTCTGGGAGTCCAACAACCG | 90 |
OsSBEⅠ | XM_015787625.1 | CGTGGCCTGGAAAGGTCAA | TCAGGCATTTCTGGTCGAGGC | 183 |
OsSBEⅡ | XM_015772017.1 | CCGCAGGAGAAATCCCATAC | GGAAGCACCTCATCCCTAA | 181 |
OsActin1 | AK100267 | TCTCAACCCCAAGGCCAATC | ATGAGTAACCACGCTCCGTC | 272 |
表1 qRT-PCR目的基因引物
Table 1 Target gene and sequence of qRT-PCR primers.
基因名称 Gene name | 登录号 Accession number | 上游引物(5′-3′) Forward primer(5′-3′) | 下游引物(5′-3′) Reverse primer(5′-3′) | 产物片段 Production size/bp |
---|---|---|---|---|
OsGBSSⅠ | XM_015789006.1 | AGCGTTGTGGCTGAGATCAA | CTTCTCACCGGTCTTTCCCC | 138 |
OsISAⅠ | XM_015794756.1 | GTGGCCTTCTCCACGAAAGA | CTCTGGGAGTCCAACAACCG | 90 |
OsSBEⅠ | XM_015787625.1 | CGTGGCCTGGAAAGGTCAA | TCAGGCATTTCTGGTCGAGGC | 183 |
OsSBEⅡ | XM_015772017.1 | CCGCAGGAGAAATCCCATAC | GGAAGCACCTCATCCCTAA | 181 |
OsActin1 | AK100267 | TCTCAACCCCAAGGCCAATC | ATGAGTAACCACGCTCCGTC | 272 |
品种 Variety | 处理 Treatment | 结实率 Seed-setting rate/% | 增幅 Increased/% | 千粒重 1000-grain weight/g | 增幅 Increased/% |
---|---|---|---|---|---|
东农423 Dongnong 423 | 施氮肥Fertilization | 96.11 aA | 1.15 | 26.77 aA | 2.17 |
对照Contrast | 95.02 bB | 0.00 | 26.20 bB | 0.00 | |
东农1706 Dongnong 1706 | 施氮肥Fertilization | 95.48 aA | 1.66 | 27.62 aA | 5.74 |
对照Contrast | 93.92 bB | 0.00 | 26.12 bB | 0.00 | |
藤系180 Toukei 180 | 施氮肥Fertilization | 86.18 aA | 1.99 | 31.39 aA | 2.90 |
对照Contrast | 84.50 bB | 0.00 | 30.48 bB | 0.00 | |
东农1712 Dongnong 1712 | 施氮肥Fertilization | 97.81 aA | 0.34 | 26.23 aA | 3.47 |
对照Contrast | 97.48 aA | 0.00 | 25.35 bB | 0.00 |
表2 灌浆成熟期氮素营养对水稻产量性状的影响
Table 2 Effect of nitrogen nutrition on rice grain yield during grain filling stage.
品种 Variety | 处理 Treatment | 结实率 Seed-setting rate/% | 增幅 Increased/% | 千粒重 1000-grain weight/g | 增幅 Increased/% |
---|---|---|---|---|---|
东农423 Dongnong 423 | 施氮肥Fertilization | 96.11 aA | 1.15 | 26.77 aA | 2.17 |
对照Contrast | 95.02 bB | 0.00 | 26.20 bB | 0.00 | |
东农1706 Dongnong 1706 | 施氮肥Fertilization | 95.48 aA | 1.66 | 27.62 aA | 5.74 |
对照Contrast | 93.92 bB | 0.00 | 26.12 bB | 0.00 | |
藤系180 Toukei 180 | 施氮肥Fertilization | 86.18 aA | 1.99 | 31.39 aA | 2.90 |
对照Contrast | 84.50 bB | 0.00 | 30.48 bB | 0.00 | |
东农1712 Dongnong 1712 | 施氮肥Fertilization | 97.81 aA | 0.34 | 26.23 aA | 3.47 |
对照Contrast | 97.48 aA | 0.00 | 25.35 bB | 0.00 |
品种与处理 Variety and treatment | 直链淀粉含量 Amylose content | 支链淀粉含量 Amylopectin content | 总淀粉含量 Starch content | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
10 DAF | 20 DAF | 30 DAF | 10 DAF | 20 DAF | 30 DAF | 10 DAF | 20 DAF | 30 DAF | |||
东农423 Dongnong 423 | |||||||||||
施氮肥Fertilization | 6.30 cC | 15.50 bB | 15.84 dC | 42.64 eE | 56.98 eE | 59.46 eE | 48.94 fF | 72.48 cC | 75.30 bB | ||
对照Contrast | 6.42 cC | 15.93 bB | 16.29 cC | 41.76 fF | 55.46 gG | 56.94 Ff | 48.29 gG | 71.08 dD | 72.91 eD | ||
增幅Increased/% | -1.87 | -2.70 | -2.76 | 2.11 | 2.74 | 4.43 | 1.35 | 1.97 | 3.28 | ||
东农1706 Dongnong 1706 | |||||||||||
施氮肥Fertilization | 9.74 bB | 16.91 cC | 17.88 bB | 46.25 cC | 60.63 bB | 61.58 cC | 56.00 aA | 75.24 aA | 76.46 aA | ||
对照Contrast | 10.05 aA | 17.87 aA | 19.20 aA | 45.00 dD | 56.62 fF | 56.86 gFG | 55.54 bB | 74.49 bB | 75.16 cB | ||
增幅Increased/% | -3.08 | -5.37 | -6.88 | 2.78 | 7.08 | 8.30 | 0.83 | 1.01 | 1.73 | ||
藤系180 Toukei 180 | |||||||||||
施氮肥Fertilization | 3.53 dD | 7.55 eE | 7.75 fDE | 50.75 aA | 61.56 aA | 66.21 aA | 54.27 cC | 68.81 eE | 73.96 dC | ||
对照Contrast | 3.70 dD | 8.00 dD | 8.27 fE | 48.69 bB | 59.26 cC | 63.67 bB | 52.58 dD | 66.76 fF | 71.33 fE | ||
增幅Increased/% | -4.59 | -5.63 | -6.29 | 4.23 | 3.88 | 3.99 | 3.21 | 3.07 | 3.69 | ||
东农1712 Dongnong 1712 | |||||||||||
施氮肥Fertilization | 2.56 gF | 5.05 gG | 7.28 gF | 48.48 bB | 59.16 dD | 59.87 dD | 50.64 eE | 64.11 gG | 67.15 gF | ||
对照Contrast | 2.71 fE | 5.46 fF | 7.88 eD | 46.05 cC | 56.61 fF | 56.69 hG | 48.76 fF | 62.08 hH | 63.57 hG | ||
增幅Increased/% | -5.54 | -7.51 | -7.61 | 5.28 | 4.50 | 5.61 | 3.86 | 3.27 | 5.63 |
表3 灌浆不同时期处理间籽粒中总淀粉、直链淀粉、支链淀粉含量应比较
Table 3 Comparison of starch, amylose, amylopectin contents in grains during different grain filling stage.
品种与处理 Variety and treatment | 直链淀粉含量 Amylose content | 支链淀粉含量 Amylopectin content | 总淀粉含量 Starch content | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
10 DAF | 20 DAF | 30 DAF | 10 DAF | 20 DAF | 30 DAF | 10 DAF | 20 DAF | 30 DAF | |||
东农423 Dongnong 423 | |||||||||||
施氮肥Fertilization | 6.30 cC | 15.50 bB | 15.84 dC | 42.64 eE | 56.98 eE | 59.46 eE | 48.94 fF | 72.48 cC | 75.30 bB | ||
对照Contrast | 6.42 cC | 15.93 bB | 16.29 cC | 41.76 fF | 55.46 gG | 56.94 Ff | 48.29 gG | 71.08 dD | 72.91 eD | ||
增幅Increased/% | -1.87 | -2.70 | -2.76 | 2.11 | 2.74 | 4.43 | 1.35 | 1.97 | 3.28 | ||
东农1706 Dongnong 1706 | |||||||||||
施氮肥Fertilization | 9.74 bB | 16.91 cC | 17.88 bB | 46.25 cC | 60.63 bB | 61.58 cC | 56.00 aA | 75.24 aA | 76.46 aA | ||
对照Contrast | 10.05 aA | 17.87 aA | 19.20 aA | 45.00 dD | 56.62 fF | 56.86 gFG | 55.54 bB | 74.49 bB | 75.16 cB | ||
增幅Increased/% | -3.08 | -5.37 | -6.88 | 2.78 | 7.08 | 8.30 | 0.83 | 1.01 | 1.73 | ||
藤系180 Toukei 180 | |||||||||||
施氮肥Fertilization | 3.53 dD | 7.55 eE | 7.75 fDE | 50.75 aA | 61.56 aA | 66.21 aA | 54.27 cC | 68.81 eE | 73.96 dC | ||
对照Contrast | 3.70 dD | 8.00 dD | 8.27 fE | 48.69 bB | 59.26 cC | 63.67 bB | 52.58 dD | 66.76 fF | 71.33 fE | ||
增幅Increased/% | -4.59 | -5.63 | -6.29 | 4.23 | 3.88 | 3.99 | 3.21 | 3.07 | 3.69 | ||
东农1712 Dongnong 1712 | |||||||||||
施氮肥Fertilization | 2.56 gF | 5.05 gG | 7.28 gF | 48.48 bB | 59.16 dD | 59.87 dD | 50.64 eE | 64.11 gG | 67.15 gF | ||
对照Contrast | 2.71 fE | 5.46 fF | 7.88 eD | 46.05 cC | 56.61 fF | 56.69 hG | 48.76 fF | 62.08 hH | 63.57 hG | ||
增幅Increased/% | -5.54 | -7.51 | -7.61 | 5.28 | 4.50 | 5.61 | 3.86 | 3.27 | 5.63 |
品种 Variety | 施肥处理 Treatment | 处理 Treatment | 最高黏度 Max. viscosity | 最低黏度 Min. viscosity | 最终黏度 Final viscosity | 崩解值 Breakdown | 消减值 Setback | 蛋白质含量 Protein / % | 直链淀粉含量 Amylose / % |
---|---|---|---|---|---|---|---|---|---|
东农423 Dongnong 423 | 施氮肥 Fertilization | 水解Hydrolyzed | 321.5 gG | 114.8 kK | 225.2 jI | 206.8 eE | -96.4 hG | 2.57 fE | 22.45 aA |
对照Contrast | 263.0 lL | 174.5 dD | 285.2 dD | 88.6 lL | 22.2 dC | 10.28 aA | 20.80 cD | ||
对照 Contrast | 水解Hydrolyzed | 279.8 kJ | 125.1 hH | 241.9 iH | 154.7 jJ | -37.9 gF | 2.52 fE | 22.59 aA | |
对照Contrast | 277.8 kK | 198.9 aA | 322.8 aA | 79.0 mM | 45.0 cB | 8.90 cB | 20.94 cC | ||
东农1706 Dongnong 1706 | 施氮肥 Fertilization | 水解Hydrolyzed | 287.0 iI | 127.9 gG | 257.8 eE | 159.1 iI | -29.2 fE | 2.48 fE | 21.87 bB |
对照Contrast | 221.3 nN | 164.2 fF | 286.3 dD | 57.1 oO | 65.0 aA | 8.98 cB | 20.41 dD | ||
对照 Contrast | 水解Hydrolyzed | 282.5 jJ | 118.0 jI | 254.4 fF | 164.5 hH | -28.1 fE | 2.50 fE | 21.92 bB | |
对照Contrast | 248.4 mM | 178.2 cC | 310.1 cC | 70.2 nN | 61.7 bA | 7.59 eD | 20.86 cD | ||
藤系180 Toukei 180 | 施氮肥 Fertilizaton | 水解Hydrolyzed | 407.5 bB | 110.0 lL | 181.4 nM | 297.5 bB | -226.1 nM | 2.54 fE | 12.21 eF |
对照Contrast | 350.1 eE | 186.7 bB | 248.9 gG | 163.4 hH | -101.2 iH | 9.40 bB | 11.44 jK | ||
对照 Contrast | 水解Hydrolyzed | 430.3 aA | 113.3 kK | 194.8 mL | 317.0 aA | -235.5 oN | 2.64 fE | 12.64 eE | |
对照Contrast | 359.1 dD | 185.4 bB | 246.2 hG | 173.7 fF | -112.9 jI | 8.48 dC | 11.80 hH | ||
东农1712 Dongnong 1712 | 施氮肥 Fertilization | 水解Hydrolyzed | 403.7 cC | 120.1 iI | 195.6 mL | 283.6 cC | -208.1 mL | 2.48 fE | 12.02 gG |
对照Contrast | 315.2 hH | 199.4 aA | 318.1 bB | 115.9 kK | 2.9 eD | 9.04 cB | 11.15 kK | ||
对照 Contrast | 水解Hydrolyzed | 348.8 eE | 117.2 jJ | 214.7 lK | 231.6 dD | -134.1 lK | 2.59 fE | 12.53 fG | |
对照Contrast | 338.6 fF | 169.4 eE | 220.9 kJ | 169.3 hH | -117.8 kJ | 8.95 cB | 11.67 iI |
表4 蛋白质水解对稻米RVA谱特性的影响
Table 4 Comparison of RVA properties of de-protein rice.
品种 Variety | 施肥处理 Treatment | 处理 Treatment | 最高黏度 Max. viscosity | 最低黏度 Min. viscosity | 最终黏度 Final viscosity | 崩解值 Breakdown | 消减值 Setback | 蛋白质含量 Protein / % | 直链淀粉含量 Amylose / % |
---|---|---|---|---|---|---|---|---|---|
东农423 Dongnong 423 | 施氮肥 Fertilization | 水解Hydrolyzed | 321.5 gG | 114.8 kK | 225.2 jI | 206.8 eE | -96.4 hG | 2.57 fE | 22.45 aA |
对照Contrast | 263.0 lL | 174.5 dD | 285.2 dD | 88.6 lL | 22.2 dC | 10.28 aA | 20.80 cD | ||
对照 Contrast | 水解Hydrolyzed | 279.8 kJ | 125.1 hH | 241.9 iH | 154.7 jJ | -37.9 gF | 2.52 fE | 22.59 aA | |
对照Contrast | 277.8 kK | 198.9 aA | 322.8 aA | 79.0 mM | 45.0 cB | 8.90 cB | 20.94 cC | ||
东农1706 Dongnong 1706 | 施氮肥 Fertilization | 水解Hydrolyzed | 287.0 iI | 127.9 gG | 257.8 eE | 159.1 iI | -29.2 fE | 2.48 fE | 21.87 bB |
对照Contrast | 221.3 nN | 164.2 fF | 286.3 dD | 57.1 oO | 65.0 aA | 8.98 cB | 20.41 dD | ||
对照 Contrast | 水解Hydrolyzed | 282.5 jJ | 118.0 jI | 254.4 fF | 164.5 hH | -28.1 fE | 2.50 fE | 21.92 bB | |
对照Contrast | 248.4 mM | 178.2 cC | 310.1 cC | 70.2 nN | 61.7 bA | 7.59 eD | 20.86 cD | ||
藤系180 Toukei 180 | 施氮肥 Fertilizaton | 水解Hydrolyzed | 407.5 bB | 110.0 lL | 181.4 nM | 297.5 bB | -226.1 nM | 2.54 fE | 12.21 eF |
对照Contrast | 350.1 eE | 186.7 bB | 248.9 gG | 163.4 hH | -101.2 iH | 9.40 bB | 11.44 jK | ||
对照 Contrast | 水解Hydrolyzed | 430.3 aA | 113.3 kK | 194.8 mL | 317.0 aA | -235.5 oN | 2.64 fE | 12.64 eE | |
对照Contrast | 359.1 dD | 185.4 bB | 246.2 hG | 173.7 fF | -112.9 jI | 8.48 dC | 11.80 hH | ||
东农1712 Dongnong 1712 | 施氮肥 Fertilization | 水解Hydrolyzed | 403.7 cC | 120.1 iI | 195.6 mL | 283.6 cC | -208.1 mL | 2.48 fE | 12.02 gG |
对照Contrast | 315.2 hH | 199.4 aA | 318.1 bB | 115.9 kK | 2.9 eD | 9.04 cB | 11.15 kK | ||
对照 Contrast | 水解Hydrolyzed | 348.8 eE | 117.2 jJ | 214.7 lK | 231.6 dD | -134.1 lK | 2.59 fE | 12.53 fG | |
对照Contrast | 338.6 fF | 169.4 eE | 220.9 kJ | 169.3 hH | -117.8 kJ | 8.95 cB | 11.67 iI |
酶活性 Enzyme activity | 品种 Variety | 处理 Treatment | 抽穗后天数 Days after heading/d | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
10 | 15 | 20 | 25 | 30 | ||||||||||||||
SuSy | 东农423 | 施氮肥Fertilization | 46.89 aA | 48.75 cC | 52.93 cB | 37.54 cC | 35.81dD | |||||||||||
Dongnong 423 | 对照Contrast | 40.85 cC | 42.54 eE | 44.63 fE | 33.55 dD | 32.60 fF | ||||||||||||
东农1706 | 施氮肥Fertilization | 40.28 dD | 67.15 aA | 56.17 aA | 48.38 bB | 47.24 bB | ||||||||||||
Dongnong 1706 | 对照Contrast | 31.30 gF | 38.43 hG | 42.09 gF | 37.47 cC | 36.86 eE | ||||||||||||
藤系180 | 施氮肥Fertilization | 39.48 fE | 41.46 fE | 50.61 dC | 48.07 bB | 46.06 fF | ||||||||||||
Toukei 180 | 对照Contrast | 30.01 hG | 40.05 gF | 35.69 hG | 29.17 fF | 28.03 hH | ||||||||||||
东农1712 | 施氮肥Fertilization | 46.42 bB | 53.63 bB | 55.58 bA | 51.82 aA | 50.69 aA | ||||||||||||
Dongnong 1712 | 对照Contrast | 39.93 eD | 45.87 dD | 46.75 eD | 30.81 eE | 29.19 gG | ||||||||||||
SPS | 东农423 | 施氮肥Fertilization | 2.03 gG | 5.81 gF | 1.71gF | 1.54 eE | 1.57 eD | |||||||||||
Dongnong 423 | 对照Contrast | 8.07 dD | 11.01 bB | 3.18 eE | 2.74 cC | 1.81 dC | ||||||||||||
东农1706 | 施氮肥Fertilization | 3.58 eE | 8.54 eD | 2.82 fE | 2.75 cC | 1.97 cC | ||||||||||||
Dongnong 1706 | 对照Contrast | 13.27 aA | 14.63 aA | 3.71 dD | 2.01 dD | 1.25 fE | ||||||||||||
藤系180 | 施氮肥Fertilization | 10.34 cC | 6.97 fE | 4.90 cC | 4.15 bB | 2.98 bB | ||||||||||||
Toukei 180 | 对照Contrast | 10.93 bB | 9.59 cC | 8.91 aA | 6.22 aA | 3.23 aA | ||||||||||||
东农1712 | 施氮肥Fertilization | 3.23 fF | 2.53 hG | 1.42 hF | 1.21 fF | 0.87 gF | ||||||||||||
Dongnong 1712 | 对照Contrast | 10.35 cC | 9.39 dC | 6.52 bB | 4.01 bB | 2.88 bB | ||||||||||||
AI | 东农423 | 施氮肥Fertilization | 12.07 bB | 14.04 cC | 7.92 aA | 7.01 aA | 3.90 cB | |||||||||||
Dongnong 423 | 对照Contrast | 4.44 gG | 5.08 hG | 2.67 bB | 2.23 hH | 1.02 hF | ||||||||||||
东农1706 | 施氮肥Fertilization | 10.02 cC | 18.05 aA | 7.39 bB | 4.06 dD | 1.68 gE | ||||||||||||
Dongnong 1706 | 对照Contrast | 4.51 gG | 6.58 fE | 3.78 gF | 2.32 gG | 1.99 fE | ||||||||||||
藤系180 | 施氮肥Fertilization | 14.35 aA | 14.36 bB | 6.67 cC | 6.07 bB | 4.22 bB | ||||||||||||
Toukei 180 | 对照Contrast | 4.77 fF | 6.59 eE | 6.02 dD | 3.26 eE | 3.06 dC | ||||||||||||
东农1712 | 施氮肥Fertilization | 9.30 dD | 9.66 dD | 5.83 eD | 5.19 cC | 4.98 aA | ||||||||||||
Dongnong 1712 | 对照Contrast | 5.38 eE | 5.51 gF | 4.23 fE | 2.97 dD | 2.45 eD |
表5 灌浆不同时期处理间籽粒蔗糖合酶(SuSy)、蔗糖磷酸合酶(SPS)和蔗糖酸性转化酶(AI)活性比较
Table 5 Comparison of SuSy, SPS and AI activities in grains under various treatments during different grain filling periods. mol/(g·min)
酶活性 Enzyme activity | 品种 Variety | 处理 Treatment | 抽穗后天数 Days after heading/d | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
10 | 15 | 20 | 25 | 30 | ||||||||||||||
SuSy | 东农423 | 施氮肥Fertilization | 46.89 aA | 48.75 cC | 52.93 cB | 37.54 cC | 35.81dD | |||||||||||
Dongnong 423 | 对照Contrast | 40.85 cC | 42.54 eE | 44.63 fE | 33.55 dD | 32.60 fF | ||||||||||||
东农1706 | 施氮肥Fertilization | 40.28 dD | 67.15 aA | 56.17 aA | 48.38 bB | 47.24 bB | ||||||||||||
Dongnong 1706 | 对照Contrast | 31.30 gF | 38.43 hG | 42.09 gF | 37.47 cC | 36.86 eE | ||||||||||||
藤系180 | 施氮肥Fertilization | 39.48 fE | 41.46 fE | 50.61 dC | 48.07 bB | 46.06 fF | ||||||||||||
Toukei 180 | 对照Contrast | 30.01 hG | 40.05 gF | 35.69 hG | 29.17 fF | 28.03 hH | ||||||||||||
东农1712 | 施氮肥Fertilization | 46.42 bB | 53.63 bB | 55.58 bA | 51.82 aA | 50.69 aA | ||||||||||||
Dongnong 1712 | 对照Contrast | 39.93 eD | 45.87 dD | 46.75 eD | 30.81 eE | 29.19 gG | ||||||||||||
SPS | 东农423 | 施氮肥Fertilization | 2.03 gG | 5.81 gF | 1.71gF | 1.54 eE | 1.57 eD | |||||||||||
Dongnong 423 | 对照Contrast | 8.07 dD | 11.01 bB | 3.18 eE | 2.74 cC | 1.81 dC | ||||||||||||
东农1706 | 施氮肥Fertilization | 3.58 eE | 8.54 eD | 2.82 fE | 2.75 cC | 1.97 cC | ||||||||||||
Dongnong 1706 | 对照Contrast | 13.27 aA | 14.63 aA | 3.71 dD | 2.01 dD | 1.25 fE | ||||||||||||
藤系180 | 施氮肥Fertilization | 10.34 cC | 6.97 fE | 4.90 cC | 4.15 bB | 2.98 bB | ||||||||||||
Toukei 180 | 对照Contrast | 10.93 bB | 9.59 cC | 8.91 aA | 6.22 aA | 3.23 aA | ||||||||||||
东农1712 | 施氮肥Fertilization | 3.23 fF | 2.53 hG | 1.42 hF | 1.21 fF | 0.87 gF | ||||||||||||
Dongnong 1712 | 对照Contrast | 10.35 cC | 9.39 dC | 6.52 bB | 4.01 bB | 2.88 bB | ||||||||||||
AI | 东农423 | 施氮肥Fertilization | 12.07 bB | 14.04 cC | 7.92 aA | 7.01 aA | 3.90 cB | |||||||||||
Dongnong 423 | 对照Contrast | 4.44 gG | 5.08 hG | 2.67 bB | 2.23 hH | 1.02 hF | ||||||||||||
东农1706 | 施氮肥Fertilization | 10.02 cC | 18.05 aA | 7.39 bB | 4.06 dD | 1.68 gE | ||||||||||||
Dongnong 1706 | 对照Contrast | 4.51 gG | 6.58 fE | 3.78 gF | 2.32 gG | 1.99 fE | ||||||||||||
藤系180 | 施氮肥Fertilization | 14.35 aA | 14.36 bB | 6.67 cC | 6.07 bB | 4.22 bB | ||||||||||||
Toukei 180 | 对照Contrast | 4.77 fF | 6.59 eE | 6.02 dD | 3.26 eE | 3.06 dC | ||||||||||||
东农1712 | 施氮肥Fertilization | 9.30 dD | 9.66 dD | 5.83 eD | 5.19 cC | 4.98 aA | ||||||||||||
Dongnong 1712 | 对照Contrast | 5.38 eE | 5.51 gF | 4.23 fE | 2.97 dD | 2.45 eD |
品种 Variety | 处理 Treatment | 抽穗后天数 Days after heading/d | GBSSⅠ | ISAⅠ | SBEⅠ | SBEⅡ |
---|---|---|---|---|---|---|
东农423 Dongnong 423 | 施氮肥Fertilization | 10 | 1.000 fF | 1.000 bB | 1.000 eD | 1.000 bB |
20 | 7.157 aA | 2.275 aA | 6.864 bA | 3.703 aA | ||
30 | 2.638 dD | 0.074 dD | 4.293 cB | 0.748 cC | ||
对照Contrast | 10 | 3.584 bB | 0.162 cC | 1.110 dC | 0.425 dD | |
20 | 3.171 cC | 0.154 cC | 6.935 aA | 3.746 aA | ||
30 | 2.125 eE | 0.004 eD | 0.864 fE | 3.750 aA | ||
东农1706 Dongnong 1706 | 施氮肥Fertilization | 10 | 1.000 fE | 1.000 bB | 1.000 fF | 1.000 dD |
20 | 3.463 bB | 3.216 aA | 3.386 bB | 1.643 cC | ||
30 | 1.422 dD | 0.983 bB | 2.490 cC | 1.638 cC | ||
对照Contrast | 10 | 3.748 aA | 0.778 cC | 1.361 eE | 1.029 dD | |
20 | 2.695 cC | 0.670 dC | 4.544 aA | 1.929 bB | ||
30 | 1.338 eD | 0.217 eD | 1.855 dD | 2.412 aA | ||
藤系180 Toukei 180 | 施氮肥Fertilization | 10 | 1.000 eE | 1.000 bB | 1.000 cC | 1.000 cC |
20 | 5.579 bB | 1.305 aA | 1.788 aA | 2.119 bB | ||
30 | 4.777 cC | 0.019 dD | 0.369 dD | 0.074 eE | ||
对照Contrast | 10 | 5.757 aA | 0.491 cC | 1.360 bB | 0.290 dD | |
20 | 5.522 bB | 0.432 cC | 1.373 bB | 2.301 aA | ||
30 | 4.572 dD | 0.006 dD | 0.126 eE | 2.319 aA | ||
东农1712 Dongnong 1712 | 施氮肥Fertilization | 10 | 1.000 bB | 1.000 aA | 1.000 dD | 1.000 dD |
20 | 4.965 aA | 0.282 cB | 6.737 bB | 3.481 bB | ||
30 | 0.686 dD | 0.005 dC | 0.701 fF | 1.165 cC | ||
对照Contrast | 10 | 1.051 bB | 0.909 bA | 2.269 cC | 0.936 dD | |
20 | 0.879 cC | 0.008 dC | 6.831 aA | 3.511 bB | ||
30 | 0.479 eE | 0.003 dC | 0.835 eE | 3.732 aA |
表6 灌浆不同时期籽粒OsGBSSⅠ、OsISAⅠ、OsSBEⅠ、OsSBEⅡ基因转录表达量比较
Table 6 Comparation of expression levels of OsGBSSⅠ、OsISAⅠ、OsSBEⅠ、OsSBEⅡ in grains under different nitrogen nutrition conditions at different filling stages.
品种 Variety | 处理 Treatment | 抽穗后天数 Days after heading/d | GBSSⅠ | ISAⅠ | SBEⅠ | SBEⅡ |
---|---|---|---|---|---|---|
东农423 Dongnong 423 | 施氮肥Fertilization | 10 | 1.000 fF | 1.000 bB | 1.000 eD | 1.000 bB |
20 | 7.157 aA | 2.275 aA | 6.864 bA | 3.703 aA | ||
30 | 2.638 dD | 0.074 dD | 4.293 cB | 0.748 cC | ||
对照Contrast | 10 | 3.584 bB | 0.162 cC | 1.110 dC | 0.425 dD | |
20 | 3.171 cC | 0.154 cC | 6.935 aA | 3.746 aA | ||
30 | 2.125 eE | 0.004 eD | 0.864 fE | 3.750 aA | ||
东农1706 Dongnong 1706 | 施氮肥Fertilization | 10 | 1.000 fE | 1.000 bB | 1.000 fF | 1.000 dD |
20 | 3.463 bB | 3.216 aA | 3.386 bB | 1.643 cC | ||
30 | 1.422 dD | 0.983 bB | 2.490 cC | 1.638 cC | ||
对照Contrast | 10 | 3.748 aA | 0.778 cC | 1.361 eE | 1.029 dD | |
20 | 2.695 cC | 0.670 dC | 4.544 aA | 1.929 bB | ||
30 | 1.338 eD | 0.217 eD | 1.855 dD | 2.412 aA | ||
藤系180 Toukei 180 | 施氮肥Fertilization | 10 | 1.000 eE | 1.000 bB | 1.000 cC | 1.000 cC |
20 | 5.579 bB | 1.305 aA | 1.788 aA | 2.119 bB | ||
30 | 4.777 cC | 0.019 dD | 0.369 dD | 0.074 eE | ||
对照Contrast | 10 | 5.757 aA | 0.491 cC | 1.360 bB | 0.290 dD | |
20 | 5.522 bB | 0.432 cC | 1.373 bB | 2.301 aA | ||
30 | 4.572 dD | 0.006 dD | 0.126 eE | 2.319 aA | ||
东农1712 Dongnong 1712 | 施氮肥Fertilization | 10 | 1.000 bB | 1.000 aA | 1.000 dD | 1.000 dD |
20 | 4.965 aA | 0.282 cB | 6.737 bB | 3.481 bB | ||
30 | 0.686 dD | 0.005 dC | 0.701 fF | 1.165 cC | ||
对照Contrast | 10 | 1.051 bB | 0.909 bA | 2.269 cC | 0.936 dD | |
20 | 0.879 cC | 0.008 dC | 6.831 aA | 3.511 bB | ||
30 | 0.479 eE | 0.003 dC | 0.835 eE | 3.732 aA |
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