中国水稻科学 ›› 2016, Vol. 30 ›› Issue (2): 127-135.DOI: 10.16819/j.1001-7216.2016.5162
彭应财1,2, 刘文真2, 傅亚萍2, 王鹤潼1, 胡国成2, 陈温福1, 徐正进1,*()
收稿日期:
2015-11-02
修回日期:
2015-11-22
出版日期:
2016-03-10
发布日期:
2016-03-10
通讯作者:
徐正进
作者简介:
# 共同第一作者;
基金资助:
Ying-cai PENG1,2, Wen-zhen LIU2, Ya-ping FU2, He-tong WANG1, Guo-cheng HU2, Wen-fu CHEN1, Zheng-jin XU1,*()
Received:
2015-11-02
Revised:
2015-11-22
Online:
2016-03-10
Published:
2016-03-10
Contact:
Zheng-jin XU
About author:
# These authors contributed equally to this work;
摘要:
从三交组合Ⅱ-32B//协青早B/Dular的F2群体中获得了1个脆性突变体,整个植株表现全生育期脆性。根据该突变体的表型,将其命名为Bc18(Brittle culm 18)。为了更好地鉴定该突变体,用正常茎秆强度品种中9B作轮回亲本与Bc18杂交,创制了Bc18脆秆近等基因系中脆B和中9B。表型鉴定显示,突变体Bc18在生育期、株高、单株穗数、每穗粒数、结实率和千粒重等主要农艺和产量性状上与野生型中9B 无显著差别,但茎、叶的机械强度分别下降了70.70%和47.16%。细胞壁组分分析表明,突变体Bc18茎、叶的纤维素和木质素含量与野生型中9B 无显著差异,但半纤维素含量分别提高了31.84%和17.35%。6个杂交组合F2和12个回交BC1F1群体的遗传分析证明Bc18 脆性突变由单显性基因控制。采用图位克隆技术,构建了Bc18/02428和Bc18/9311的F2定位群体,并利用网上公布的SSR标记和新设计的InDel标记,最终将Bc18基因定位在第1染色体长臂端InDel标记PBC22与PBC33之间约154 kb的区间内。
中图分类号:
彭应财, 刘文真, 傅亚萍, 王鹤潼, 胡国成, 陈温福, 徐正进. 水稻显性脆秆突变体Bc18的鉴定和基因定位[J]. 中国水稻科学, 2016, 30(2): 127-135.
Ying-cai PENG, Wen-zhen LIU, Ya-ping FU, He-tong WANG, Guo-cheng HU, Wen-fu CHEN, Zheng-jin XU. Characterization and Gene Mapping of a Dominant Brittle Culm Mutant Bc18 in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2016, 30(2): 127-135.
材料 Material | 播始历期 DSH/d | 株 高 PH/cm | 茎秆粗度 ODS/mm | 茎秆壁厚 TS/mm | 叶长 LL/cm | 叶宽 LW/cm | 叶厚 TL/mm |
---|---|---|---|---|---|---|---|
Bc18 | 64 | 92.83±1.75 a | 5.24±0.52 a | 1.69±0.46 a | 47.20±4.13 a | 1.14±0.11 a | 0.28±0.05 a |
WT | 64 | 92.66±2.28 a | 5.47±0.79 a | 1.49±0.48 a | 43.20±7.25 a | 1.26±0.11 a | 0.23±0.04 a |
表1 突变体Bc18及其野生型的主要特征特性(2008年,杭州)
Table 1 Major agronomic traits of Bc18 and its wild type in Hangzhou, 2008.
材料 Material | 播始历期 DSH/d | 株 高 PH/cm | 茎秆粗度 ODS/mm | 茎秆壁厚 TS/mm | 叶长 LL/cm | 叶宽 LW/cm | 叶厚 TL/mm |
---|---|---|---|---|---|---|---|
Bc18 | 64 | 92.83±1.75 a | 5.24±0.52 a | 1.69±0.46 a | 47.20±4.13 a | 1.14±0.11 a | 0.28±0.05 a |
WT | 64 | 92.66±2.28 a | 5.47±0.79 a | 1.49±0.48 a | 43.20±7.25 a | 1.26±0.11 a | 0.23±0.04 a |
材料 Material | 年份 Year | 单株有效穗 No. of effective panicles per plant | 每穗总粒数 Spikelet number per panicle | 每穗实粒数 Filled grain number per panicle | 结实率 Seed-setting rate/% | 穗长 Panicle length /cm | 千粒重 1000-grain weight /g | 产量 Yield /(kg·666.7m-2) |
---|---|---|---|---|---|---|---|---|
Bc18 | 2008 | 11.93±0.50 | 149.16±3.33 | 116.30±6.33 | 77.96±3.47 | 22.39±0.73 | 24.17±0.30 | 431.06±10.25 |
2009 | 13.55±0.07 | 151.63±10.95 | 116.47±14.05 | 76.68±3.73 | 24.06±1.39 | 24.03±0.32 | 425.76±24.30 | |
平均 Average | 12.74±0.29 a | 150.40±7.14 a | 116.39±10.19 a | 77.32±3.60 a | 23.22±1.06 a | 24.10±0.31 a | 428.41±17.28 a | |
WT | 2008 | 12.43±0.78 | 156.07±8.40 | 118.42±7.94 | 75.85±1.99 | 23.23±1.03 | 24.38±0.18 | 441.67±9.46 |
2009 | 13.10±0.42 | 153.44±5.08 | 110.36±1.62 | 71.95±1.33 | 23.83±2.44 | 24.25±0.21 | 440.15±27.40 | |
平均 Average | 12.77±0.60 a | 154.75±6.74 a | 114.39±4.78 a | 73.90±1.66 a | 23.53±1.73 a | 24.32±0.19 a | 440.91±18.43 a |
表2 突变体Bc18与及其野生型的产量与产量构成(2008-2009年,杭州)
Table 2 Yields and yield components of Bc18 and its wild type in Hangzhou, 2008-2009.
材料 Material | 年份 Year | 单株有效穗 No. of effective panicles per plant | 每穗总粒数 Spikelet number per panicle | 每穗实粒数 Filled grain number per panicle | 结实率 Seed-setting rate/% | 穗长 Panicle length /cm | 千粒重 1000-grain weight /g | 产量 Yield /(kg·666.7m-2) |
---|---|---|---|---|---|---|---|---|
Bc18 | 2008 | 11.93±0.50 | 149.16±3.33 | 116.30±6.33 | 77.96±3.47 | 22.39±0.73 | 24.17±0.30 | 431.06±10.25 |
2009 | 13.55±0.07 | 151.63±10.95 | 116.47±14.05 | 76.68±3.73 | 24.06±1.39 | 24.03±0.32 | 425.76±24.30 | |
平均 Average | 12.74±0.29 a | 150.40±7.14 a | 116.39±10.19 a | 77.32±3.60 a | 23.22±1.06 a | 24.10±0.31 a | 428.41±17.28 a | |
WT | 2008 | 12.43±0.78 | 156.07±8.40 | 118.42±7.94 | 75.85±1.99 | 23.23±1.03 | 24.38±0.18 | 441.67±9.46 |
2009 | 13.10±0.42 | 153.44±5.08 | 110.36±1.62 | 71.95±1.33 | 23.83±2.44 | 24.25±0.21 | 440.15±27.40 | |
平均 Average | 12.77±0.60 a | 154.75±6.74 a | 114.39±4.78 a | 73.90±1.66 a | 23.53±1.73 a | 24.32±0.19 a | 440.91±18.43 a |
图1 突变体Bc18和野生型的表型 A、B、C和D分别为突变体Bc18易折断的茎秆、叶片、叶鞘和稻穗; 箭头表示突变体Bc18各个部分易断裂之处。
Fig. 1. Phenotypes of Bc18 and its wild type. A, B, C and D show broken culm, leaf, leaf sheath and panicle of Bc18, respectively; Arrows indicate easily broken position of Bc18; WT, Wild type.
图2 突变体Bc18和野生型的机械强度 10个测定数据的平均值;** 表示在0.01水平上差异显著;横栏表示标准误。
Fig. 2. Mechanical strength of Bc18 and its wild type. The average values are means of 10 measurements; ** significant at 0.01 level; Bars are standard errors; WT, Wild type.
图3 突变体Bc18和野生型细胞壁组分含量 A-茎秆纤维素、半纤维素和木质素含量; B-叶片纤维素、半纤维素和木质素含量。3个测定数据的平均值;**表示在0.01水平上差异显著;横栏表示标准误。
Fig. 3. Measurement of cell wall components of Bc18 and its wild type. A and B, Cellulose, hemicellulose and lignin contents of total cell wall residues of the culm ( A ) and leaf ( B ) segments from Bc18 and its wild type plants; The average values are means of 3 measurements; ** significant at 0.01 level; The bars refer standard errors.
组合 Combination | F1 | F2群体 F2 population | P值 P value | |||
---|---|---|---|---|---|---|
脆秆单株 Brittle culm | 野生型 Wild type | 总数 Total | ||||
中9B/Bc18 Zhong 9B/Bc18 | 脆秆 Brittle culm | 1278 | 419 | 1697 | 0.04 | 0.769 |
Ⅱ-32B/Bc18 | 脆秆Brittle culm | 1754 | 573 | 2327 | 0.09 | 0.675 |
Bc18/协青早B Bc18/Xieqingzao B | 脆秆 Brittle culm | 1455 | 477 | 1932 | 0.05 | 0.753 |
Bc18/明恢63 Bc18/Minghui 63 | 脆秆 Brittle culm | 1817 | 595 | 2412 | 0.07 | 0.707 |
密阳46/Bc18 Milyang 46/Bc18 | 脆秆Brittle culm | 1045 | 358 | 1403 | 0.10 | 0.655 |
Bc18/中恢218 Bc18/Zhonghui 218 | 脆秆Brittle culm | 2089 | 703 | 2792 | 0.02 | 0.827 |
表3 突变体Bc18 F2分离群体
Table 3 Genetic analyses of Bc18 F2 populations.
组合 Combination | F1 | F2群体 F2 population | P值 P value | |||
---|---|---|---|---|---|---|
脆秆单株 Brittle culm | 野生型 Wild type | 总数 Total | ||||
中9B/Bc18 Zhong 9B/Bc18 | 脆秆 Brittle culm | 1278 | 419 | 1697 | 0.04 | 0.769 |
Ⅱ-32B/Bc18 | 脆秆Brittle culm | 1754 | 573 | 2327 | 0.09 | 0.675 |
Bc18/协青早B Bc18/Xieqingzao B | 脆秆 Brittle culm | 1455 | 477 | 1932 | 0.05 | 0.753 |
Bc18/明恢63 Bc18/Minghui 63 | 脆秆 Brittle culm | 1817 | 595 | 2412 | 0.07 | 0.707 |
密阳46/Bc18 Milyang 46/Bc18 | 脆秆Brittle culm | 1045 | 358 | 1403 | 0.10 | 0.655 |
Bc18/中恢218 Bc18/Zhonghui 218 | 脆秆Brittle culm | 2089 | 703 | 2792 | 0.02 | 0.827 |
图4 与Bc18基因连锁的SSR标记RM7419的扩增结果 1-Bc18; 2-02428; 3-F1(02428×Bc18); 4~24-F2 不脆单株。
Fig. 4. Electrophoresis of the SSR marker RM7419 linked with the Bc18 locus. Lane 1, Bc18; Lane 2, 02428; Lane 3,F1 (02428/Bc18); Lanes 4 to 24, Non-brittle culm individuals in F2 population.
标记 Marker | 引物序列(5'-3') Primer sequence (5'-3') | 产物大小 Product size in Nipponbare/bp |
---|---|---|
PBC4 | F:GAACGTTTACAAGACTCT | 124 |
R:ACACAACTACTTCACTGC | ||
PBC21 | F:TCCCACCACCTCAATCTC | 116 |
R:CCCTACGACTCCAGCTAAT | ||
PBC22 | F:TCGCATATTCAGATAAGCATC | 108 |
R:TAATCGGAAAGAAGGCAAT | ||
PBC33 | F:GCTTGTTGCTGTTGCCTCTT | 151 |
R:TCACGGCAGAATCGCACA | ||
PBC31 | F:CTACAAAGGCACGATGATGA | 185 |
R:AACGCAACCTAAAAGACACTAA | ||
PBC25 | F:CTTGAGCCATTATGTGCA | 166 |
R:GACTCACGGACGCTATCA |
表4 用于Bc18基因精细定位的分子标记
Table 4 Markers used for fine mapping of the Bc18 gene.
标记 Marker | 引物序列(5'-3') Primer sequence (5'-3') | 产物大小 Product size in Nipponbare/bp |
---|---|---|
PBC4 | F:GAACGTTTACAAGACTCT | 124 |
R:ACACAACTACTTCACTGC | ||
PBC21 | F:TCCCACCACCTCAATCTC | 116 |
R:CCCTACGACTCCAGCTAAT | ||
PBC22 | F:TCGCATATTCAGATAAGCATC | 108 |
R:TAATCGGAAAGAAGGCAAT | ||
PBC33 | F:GCTTGTTGCTGTTGCCTCTT | 151 |
R:TCACGGCAGAATCGCACA | ||
PBC31 | F:CTACAAAGGCACGATGATGA | 185 |
R:AACGCAACCTAAAAGACACTAA | ||
PBC25 | F:CTTGAGCCATTATGTGCA | 166 |
R:GACTCACGGACGCTATCA |
[1] | 吴超, 傅亚萍, 朱丽, 等. 转高赖氨酸蛋白基因脆茎水稻的收获指数及秸秆赖氨酸含量的研究. 浙江农业学报, 2008, 20(4): 225-230. |
Wu C, Fu Y P, Zhu L, et al.Studies on harvest index and culm lysine content in the transgenic brittle culm rice inserted lysine-rich protein gene.Acta Agric Zhejiang, 2008, 20(4): 225-230. (in Chinese with English abstract) | |
[2] | 汪海峰, 朱军莉, 刘建新, 等. 饲喂脆茎全株水稻对生长肥育猪生长性能、养分消化和胴体质量的影响. 畜牧兽医学报, 2005, 36(11): 1139-1144. |
Wang H F, Zhu J L, Liu J X, et al.Growth performance, nutrient digestibility and carcass quality of growing-finishing pigs fed different levels of whole crop rice brittle culm-1.Acta Veter Zootech Sin, 2005, 36(11): 1139-1144. (in Chinese with English abstract) | |
[3] | 黄峰, 王永泽, 周胜德, 等. 水稻脆性秸秆发酵产纤维乙醇的研究.可再生能源, 2014, 32(2): 211-215. |
Huang F, Wang Y Z, Zhou S D, et al.Study on cellulosic ethanol fermentation of brittle rice straw.Rene Energy Res, 2014, 32(2): 211-215. (in Chinese with English abstract) | |
[4] | 冯永清, 邹维华, 李丰成, 等. 特异水稻脆茎突变体生物学特性及生物质降解效率的研究. 中国农业科技导报, 2013, 15(3): 77-83. |
Feng Y Q, Zou W H, Li F C, et al.Studies on biological characterization of rice brittle culm mutants and their biomass degradation efficiency.J Agric Sci and Technol, 2013, 15(3): 77-83. (in Chinese with English abstract) | |
[5] | Nagao S, Takahashi M.Genetieal studies on rice plant,XXVII: Trial construction of twelve linkage groups in Japanese rice.Fac Agr Hokkaido Univ, 1963, 53(1): 72-130. |
[6] | Kotake T, Aohara T, Hirano K, et al.Rice Brittle culm 6 encodes a dominant-negative form of CesA protein that perturbs cellulose synthesis in secondary cell walls.J Exper Bot, 2011, 62(6): 2053-2062. |
[7] | 王川丽, 王令强, 牟同敏. 水稻脆性突变体nbc(t)的主要特性和脆性基因的初步定位. 华中农业大学学报, 2012, 31(2): 159-164. |
Wang C L, Wang L Q,Mou T M.Characterization and gene mapping of a brittle culm mutant nbc(t) in rice.J Huazhong Agric Univ, 2012, 31(2): 159-164. (in Chinese with English abstract) | |
[8] | Tanaka K, Murata K, Yamazaki M, et al.Three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall.Plant Physiol, 2003, 133(1): 73-83. |
[9] | Yan C J, Yan S, Zeng X H, et al.Fine mapping and isolation of bc7(t), allelic to OsCesA4.J Genet and Genom, 2007, 34(11): 1019-1027. |
[10] | Zhang B C, Deng L W, Qian Q, et al.A missense mutation in the transmembrane domain of CESA4 affects protein abundance in the plasma membrane and results in abnormal cell wall biosynthesis in rice.Plant Mol Biol, 2009, 71(4-5): 509-524. |
[11] | Xu J D, Zhang Q F, Zhang T, et al.Phenotypic characterization, genetic analysis and gene-mapping for a brittle mutant in rice.J Integr Plant Biol, 2008, 50(3): 319-328. |
[12] | Rao Y C, Yang Y L, Xin D D, et al.Characterization and cloning of a brittle culm mutant (bc88) in rice (Oryza sativa L.).Chin Sci Bull, 2013, 58(24): 3000-3006. |
[13] | 李晓静, 徐多多, 徐益敏, 等. 水稻纤维素合酶催化亚基的编码基因BC88的表达分析. 中国水稻科学, 2015, 29(2): 126-134. |
Li X J, Xu D D, Xu Y M, et al.Expression of OsBC88,a rice cellulose synthase catalytic subunit gene.Chin J rice Sci,2015, 29(2): 126-134. (in Chinese with English abstract) | |
[14] | 吴国超, 桑贤春, 马娇, 等. 水稻矮脆突变体dwfl的特性与基因定位.植物遗传资源学报, 2014, 15(4): 795-801. |
Wu G C, Sang X C, Ma J, et al.Genetic analysis and fine-mapping of a dwarf and fragile mutant dwf1 in rice.J Plant Genet Resou, 2014, 15(4): 795-801. (in Chinese with English abstract) | |
[15] | Wang D F, Yuan S J, Yin L, et al.A missense mutation in the transmembrane domain of CESA9 affects cell wall biosynthesis and plant growth in rice.Plant Sci, 2012, 196(11): 117-124. |
[16] | Song X Q, Liu L F, Jiang Y J, et al.Disruption of secondary wall cellulose biosynthesis alters cadmium translocation and tolerance in rice plants.Molecular Plant, 2013, 3(6): 768-780. |
[17] | Li Y H, Qian Q, Zhou Y H, et al.Brittle Culm 1,which encodes a cobra-like protein, affects the mechanical properties of rice plants.Plant Cell, 2003, 15(9): 2020-2031. |
[18] | Liu L F, Shang-Guan K K, Zhang B C,et al. Brittle culm 1, a cobra-like protein, functions in cellulose assembly through binding cellulose microfibrils.Plos Genet, 2013, 9(8): 1159-1169. |
[19] | Xiong G Y, Li R, Qian Q, et al.The rice dynamin-related protein DRP2B mediates membrane trafficking,and thereby plays a critical role in secondary cell wall cellulose biosynthesis.Plant J, 2010, 64(1): 56-70. |
[20] | Ko H, Toshihisa K, Kumiko K, et al.Rice Brittle Culm 3 ( BC3 ) encodes a classical dynamin OsDRP2B essential for proper secondary cell wall synthesis.Planta, 2010, 232(1): 95-108. |
[21] | Zhou Y H, Li S B, Qian Q, et al.BC10, a DUF266-containing and Golgi-located type Ⅱ membrane protein, is required for cell-wall biosynthesis in rice (Oryza sativa L.).plant J, 2009, 57(3): 446-462. |
[22] | Zhang M, Zhang B C, Qian Q, et al.Brittle Culm 12, a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice.Plant J, 2010, 63(2): 312-328. |
[23] | Zhang B C, Liu X L, Qian Q, et al.Golgi nucleotide sugar transporter modulates cell wall biosynthesis and plant growth in rice.PNAS, 2011, 108(12): 5110-5115. |
[24] | Song X Q, Zhang B C, Zhou Y H.Golgi-localized UDP-glucose transporter is required for cell wall integrity in rice.Plant Signal Behav, 2011, 6(8): 1097-1100. |
[25] | Wu B, Zhang B C, Dai Y, et al.Brittle culm 15 encodes a membrane-associated chitinase-like protein required for cellulose biosynthesis in rice.Plant Physiol, 2012, 159(4): 1440-1452. |
[26] | Li X J, Yang Y, Yao J L, et al.Flexible Culm 1 encoding a cinnamyl-alcohol dehydrogenase controls culm mechanical strength in rice.Plant Mol Biol, 2009, 69(6): 685-697. |
[27] | 叶亚峰, 刘斌美, 许学, 等. 水稻脆秆矮生突变体鉴定及基因定位研究. 核农学报, 2012, 26(1): 1-5. |
Ye Y F, Liu B M, Xu X,et al.Identification and genetic mapping of a fragile and dwarf rice mutant.J Nucl Agric Sci, 2012, 26(1): 1-5. (in Chinese with English abstract) | |
[28] | 桑贤春, 杜川, 王晓雯, 等. 水稻矮秆脆性突变体dbc1的鉴定与基因定位. 作物学报, 2013, 39(4): 626-631. |
Sang X C, Du C, Wang X W, et al.Identification and gene mapping of dwarf and brittle culm mutant dbc1 in Oryza sativa.Acta Agron Sin, 2013, 39(4): 626-631. (in Chinese with English abstract) | |
[29] | Takahashi M, Kinoshita T, Takeda K.Genetical studies on rice plant,XXXIII :Character expression and causal genes of some mutants in rice plant.J Fac Agric Hokkaido Univ, 1968, 55(4): 496-512. |
[30] | Sanchez A C, Khush G S.Chromosomal location of some marker genes in rice using the primary trisomics.J Hered, 1994, 85(4): 297-300. |
[31] | Librojo A L, Khush G S.Chromosomal Location of Some Mutant Genes Through the Use of Primary Trisomics in Rice. Rice Genetics. Manila(Philippines): IRRI, 1986: 249-255. |
[32] | 张上都, 余显权, 赵福胜, 等. 一个水稻脆秆重组体的遗传分析.贵州农业科学, 2010, 38(2): 5-6. |
Zhang S D, Yu X Q, Zhao F S, et al.Genetic analysis of a rice recombination with brittle stem.Guizhou Agric Sci, 2010, 38(2): 5-6. (in Chinese with English abstract) | |
[33] | 蒋钰东, 何沛龙, 廖红香, 等. 水稻脆性及叶尖枯死突变体fld1的鉴定与基因定位. 植物学报, 2014, 49(6): 663-671. |
Jiang Y D, He P L, Liao H X, et al.Identification and gene mapping of a fragile and leaf-tip dead mutant fld1 in Oryza sativa.Chin Bull Bot,2014, 49(6): 663-671. (in Chinese with English abstract) | |
[34] | Van Soest P J, Robertson J B, Lewis A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.J Dairy Sci, 1991, 74(10): 3583-3598. |
[35] | 卢扬江, 郑康乐. 提取水稻DNA的一种简易方法. 中国水稻科学, 1992, 6(1): 47-48. |
Lu Y J, Zheng K L.A simple method for isolation of rice DNA.Chin J Rice Sci, 1992, 6(1): 47-48. (in Chinese with English abstract ) |
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