Chinese Journal OF Rice Science ›› 2024, Vol. 38 ›› Issue (5): 525-534.DOI: 10.16819/j.1001-7216.2024.230811
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YANG Hao1,#, HUANG Yanyan1,#, WANG Jian2,3, YI Chunlin1, SHI Jun4, TAN Chutian1, REN Wenrui1, WANG Wenming1,*()
Received:
2023-08-31
Revised:
2023-11-17
Online:
2024-09-10
Published:
2024-09-10
Contact:
*email: j316wenmingwang@163.com
About author:
#These authors contributed equally to the work
杨好1,#, 黄衍焱1,#, 王剑2,3, 易春霖1, 石军4, 谭楮湉1, 任文芮1, 王文明1,*()
通讯作者:
*email: j316wenmingwang@163.com
作者简介:
#共同第一作者
基金资助:
YANG Hao, HUANG Yanyan, WANG Jian, YI Chunlin, SHI Jun, TAN Chutian, REN Wenrui, WANG Wenming. Development and Application of Specific Molecular Markers for Eight Rice Blast Resistance Genes in Rice[J]. Chinese Journal OF Rice Science, 2024, 38(5): 525-534.
杨好, 黄衍焱, 王剑, 易春霖, 石军, 谭楮湉, 任文芮, 王文明. 水稻中八个稻瘟病抗性基因特异分子标记的开发及应用[J]. 中国水稻科学, 2024, 38(5): 525-534.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2024.230811
编号 No. | 水稻品种 Rice variety | 编号 No. | 水稻品种 Rice variety | 编号 No. | 水稻品种 Rice variety | 编号 No. | 水稻品种 Rice variety |
---|---|---|---|---|---|---|---|
1 | IRBLa-A (Pi-a) | 35 | 绵恢146 Mianhui 146 | 69 | 泰恢808 Taihui 808 | 103 | 德香074B Dexiang 074B |
2 | IRBLi-F5 (Pi-i) | 36 | 绵恢725 Mianhui 725 | 70 | 五山丝苗 Wushansimiao | 104 | 赣香B Ganxiang B |
3 | IRBLks-F5 (Pi-ks) | 37 | 南恢558 Nanhui 558 | 71 | 西科恢768 Xikehui 768 | 105 | 广8B Guang 8B |
4 | IRBLk-Ka (Pi-k) | 38 | 南恢968 Nanhui 968 | 72 | 雅禾 Yahe | 106 | 泸香618B Luxiang 618B |
5 | IRBLkp-K60 (Pi-kp) | 39 | 蜀恢257 Shuhui 257 | 73 | 雅恢2115 Yahui 2115 | 107 | 绵5B Mian 5B |
6 | IRBLkh-K3 (Pi-kh) | 40 | 蜀恢308 Shuhui 308 | 74 | 雅恢2116 Yahui 2116 | 108 | 忠香B Zhongxiang B |
7 | IRBLz-Fu (Pi-z) | 41 | 蜀恢451 Shuhui 451 | 75 | 雅恢2117 Yahui 2117 | 109 | 内香6B Neixiang 6B |
8 | IRBLz5-CA (Pi-z5) | 42 | 蜀恢498 Shuhui 498 | 76 | 雅恢2118 Yahui 2118 | 110 | 千乡654B Qianxiang 654B |
9 | IRBLzt-T (Pi-zt) | 43 | 蜀恢505 Shuhui 505 | 77 | 雅恢2119 Yahui 2119 | 111 | 荃9311B Quan 9311B |
10 | IRBLta-K1 (Pi-ta) | 44 | 蜀恢548 Shuhui 548 | 78 | 雅恢2199 Yahui 2199 | 112 | 蓉18B Rong 18B |
11 | IRBLb-B (Pi-b) | 45 | 蜀恢573 Shuhui 573 | 79 | 雅恢2275 Yahui 2275 | 113 | 蓉7B Rong 7B |
12 | IRBLt-K59 (Pi-t) | 46 | 蜀恢586 Shuhui 586 | 80 | 雅恢2348 Yahui 2348 | 114 | 双1B Shuang 1B |
13 | IRBLsh-B (Pi-sh) | 47 | 蜀恢589 Shuhui 589 | 81 | 雅恢2816 Yahui 2816 | 115 | 泰丰B Taifeng B |
14 | IRBL1-CL (Pi-1) | 48 | 猪毛糯 Zhumaonuo | 82 | 雅恢2918 Yahui 2918 | 116 | 武金4B Wujin 4B |
15 | IRBL3-CP4 (Pi-3) | 49 | HR2168 | 83 | 雅恢5049 Yahui 5049 | 117 | 武香B Wuxiang B |
16 | IRBL5-M (Pi-5) | 50 | R900 | 84 | 雅恢5217 Yahui 5217 | 118 | 雅1B Ya 1B |
17 | IRBL7-M (Pi-7) | 51 | R9311 | 85 | 雨恢38 Yuhui 38 | 119 | 雅3B Ya 3B |
18 | IRBL9-W (Pi-9) | 52 | 成恢19 Chenghui 19 | 86 | 岳恢9113 Yuehui 9113 | 120 | 雅7B Ya 7B |
19 | IRBL12-M (Pi-12) | 53 | 乐恢188 Lehui 188 | 87 | 粤农丝苗 Yuenongsimiao | 121 | 宜香1B Yixiang 1B |
20 | IRBL19-M (Pi-19) | 54 | 乐恢584 Lehui 584 | 88 | D297B | 122 | 泰国香稻 Thai fragrant rice |
21 | IRBLkm-Ts (Pi-km) | 55 | 泸恢17 Luhui 17 | 89 | D35B | 123 | 楚粳28 Chujing 28 |
22 | IRBL20-IR24 (Pi-20) | 56 | 泸恢8258 Luhui 8258 | 90 | D62B | 124 | 野香B Yexiang B |
23 | IRBLta2-Pi (Pi-ta2) | 57 | 绵恢523 Mianhui 523 | 91 | D79B | 125 | 绵恢6139 Mianhui 6139 |
24 | IRBL11-Zh (Pi-11) | 58 | 绵恢528 Mianhui 528 | 92 | F-32B | 126 | 绵恢313 Mianhui 313 |
25 | E33 | 59 | 绵恢662 Mianhui 662 | 93 | F59B | 127 | 绵恢357 Mianhui 357 |
26 | 成恢3203 Chenghui 3203 | 60 | 明恢63 Minghui 63 | 94 | G46B | 128 | 绵恢365 Mianhui 365 |
27 | 成恢727 Chenghui 727 | 61 | 明恢86 Minghui 86 | 95 | 川106B Chuan 106B | 129 | 绵恢821 Mianhui 821 |
28 | 成恢993 Chenghui 993 | 62 | 内恢99-14 Neihui 99-14 | 96 | 川608B Chuan 608B | 130 | 绵恢823 Mianhui 823 |
29 | 德恢6099 Dehui 6099 | 63 | 蜀恢137 Shuhui 137 | 97 | 川谷B Chuangu B | 131 | 绵恢837 Mianhui 837 |
30 | 多恢1号 Duohui 1 | 64 | 蜀恢208 Shuhui 208 | 98 | 川华B Chuanhua B | 132 | 绵恢857 Mianhui 857 |
31 | 航恢1179 Hanghui 1179 | 65 | 蜀恢527 Shuhui 527 | 99 | 川农1B Chuannong 1B | 133 | 绵恢815 Mianhui 815 |
32 | 航恢570 Hanghui 570 | 66 | 蜀恢538 Shuhui 538 | 100 | 川农4B Chuannong 4B | 134 | 日本晴 NPB |
33 | 华占 Huazhan | 67 | 蜀恢569 Shuhui 569 | 101 | 川种3B Chuanzhong 3B | 135 | 中花11 Zhonghua 11 |
34 | 乐恢433 Lehui 433 | 68 | 蜀恢882 Shuhui 882 | 102 | 德66B De 66B | 136 | 丽江新团黑谷 LTH |
Table 1. Rice varieties used in this study
编号 No. | 水稻品种 Rice variety | 编号 No. | 水稻品种 Rice variety | 编号 No. | 水稻品种 Rice variety | 编号 No. | 水稻品种 Rice variety |
---|---|---|---|---|---|---|---|
1 | IRBLa-A (Pi-a) | 35 | 绵恢146 Mianhui 146 | 69 | 泰恢808 Taihui 808 | 103 | 德香074B Dexiang 074B |
2 | IRBLi-F5 (Pi-i) | 36 | 绵恢725 Mianhui 725 | 70 | 五山丝苗 Wushansimiao | 104 | 赣香B Ganxiang B |
3 | IRBLks-F5 (Pi-ks) | 37 | 南恢558 Nanhui 558 | 71 | 西科恢768 Xikehui 768 | 105 | 广8B Guang 8B |
4 | IRBLk-Ka (Pi-k) | 38 | 南恢968 Nanhui 968 | 72 | 雅禾 Yahe | 106 | 泸香618B Luxiang 618B |
5 | IRBLkp-K60 (Pi-kp) | 39 | 蜀恢257 Shuhui 257 | 73 | 雅恢2115 Yahui 2115 | 107 | 绵5B Mian 5B |
6 | IRBLkh-K3 (Pi-kh) | 40 | 蜀恢308 Shuhui 308 | 74 | 雅恢2116 Yahui 2116 | 108 | 忠香B Zhongxiang B |
7 | IRBLz-Fu (Pi-z) | 41 | 蜀恢451 Shuhui 451 | 75 | 雅恢2117 Yahui 2117 | 109 | 内香6B Neixiang 6B |
8 | IRBLz5-CA (Pi-z5) | 42 | 蜀恢498 Shuhui 498 | 76 | 雅恢2118 Yahui 2118 | 110 | 千乡654B Qianxiang 654B |
9 | IRBLzt-T (Pi-zt) | 43 | 蜀恢505 Shuhui 505 | 77 | 雅恢2119 Yahui 2119 | 111 | 荃9311B Quan 9311B |
10 | IRBLta-K1 (Pi-ta) | 44 | 蜀恢548 Shuhui 548 | 78 | 雅恢2199 Yahui 2199 | 112 | 蓉18B Rong 18B |
11 | IRBLb-B (Pi-b) | 45 | 蜀恢573 Shuhui 573 | 79 | 雅恢2275 Yahui 2275 | 113 | 蓉7B Rong 7B |
12 | IRBLt-K59 (Pi-t) | 46 | 蜀恢586 Shuhui 586 | 80 | 雅恢2348 Yahui 2348 | 114 | 双1B Shuang 1B |
13 | IRBLsh-B (Pi-sh) | 47 | 蜀恢589 Shuhui 589 | 81 | 雅恢2816 Yahui 2816 | 115 | 泰丰B Taifeng B |
14 | IRBL1-CL (Pi-1) | 48 | 猪毛糯 Zhumaonuo | 82 | 雅恢2918 Yahui 2918 | 116 | 武金4B Wujin 4B |
15 | IRBL3-CP4 (Pi-3) | 49 | HR2168 | 83 | 雅恢5049 Yahui 5049 | 117 | 武香B Wuxiang B |
16 | IRBL5-M (Pi-5) | 50 | R900 | 84 | 雅恢5217 Yahui 5217 | 118 | 雅1B Ya 1B |
17 | IRBL7-M (Pi-7) | 51 | R9311 | 85 | 雨恢38 Yuhui 38 | 119 | 雅3B Ya 3B |
18 | IRBL9-W (Pi-9) | 52 | 成恢19 Chenghui 19 | 86 | 岳恢9113 Yuehui 9113 | 120 | 雅7B Ya 7B |
19 | IRBL12-M (Pi-12) | 53 | 乐恢188 Lehui 188 | 87 | 粤农丝苗 Yuenongsimiao | 121 | 宜香1B Yixiang 1B |
20 | IRBL19-M (Pi-19) | 54 | 乐恢584 Lehui 584 | 88 | D297B | 122 | 泰国香稻 Thai fragrant rice |
21 | IRBLkm-Ts (Pi-km) | 55 | 泸恢17 Luhui 17 | 89 | D35B | 123 | 楚粳28 Chujing 28 |
22 | IRBL20-IR24 (Pi-20) | 56 | 泸恢8258 Luhui 8258 | 90 | D62B | 124 | 野香B Yexiang B |
23 | IRBLta2-Pi (Pi-ta2) | 57 | 绵恢523 Mianhui 523 | 91 | D79B | 125 | 绵恢6139 Mianhui 6139 |
24 | IRBL11-Zh (Pi-11) | 58 | 绵恢528 Mianhui 528 | 92 | F-32B | 126 | 绵恢313 Mianhui 313 |
25 | E33 | 59 | 绵恢662 Mianhui 662 | 93 | F59B | 127 | 绵恢357 Mianhui 357 |
26 | 成恢3203 Chenghui 3203 | 60 | 明恢63 Minghui 63 | 94 | G46B | 128 | 绵恢365 Mianhui 365 |
27 | 成恢727 Chenghui 727 | 61 | 明恢86 Minghui 86 | 95 | 川106B Chuan 106B | 129 | 绵恢821 Mianhui 821 |
28 | 成恢993 Chenghui 993 | 62 | 内恢99-14 Neihui 99-14 | 96 | 川608B Chuan 608B | 130 | 绵恢823 Mianhui 823 |
29 | 德恢6099 Dehui 6099 | 63 | 蜀恢137 Shuhui 137 | 97 | 川谷B Chuangu B | 131 | 绵恢837 Mianhui 837 |
30 | 多恢1号 Duohui 1 | 64 | 蜀恢208 Shuhui 208 | 98 | 川华B Chuanhua B | 132 | 绵恢857 Mianhui 857 |
31 | 航恢1179 Hanghui 1179 | 65 | 蜀恢527 Shuhui 527 | 99 | 川农1B Chuannong 1B | 133 | 绵恢815 Mianhui 815 |
32 | 航恢570 Hanghui 570 | 66 | 蜀恢538 Shuhui 538 | 100 | 川农4B Chuannong 4B | 134 | 日本晴 NPB |
33 | 华占 Huazhan | 67 | 蜀恢569 Shuhui 569 | 101 | 川种3B Chuanzhong 3B | 135 | 中花11 Zhonghua 11 |
34 | 乐恢433 Lehui 433 | 68 | 蜀恢882 Shuhui 882 | 102 | 德66B De 66B | 136 | 丽江新团黑谷 LTH |
序号 No. | R基因 R gene | 引物名称 Primer | 引物序列 Sequence | PCR产物长度 Size of PCR production(bp) | PCR退火温度 Annealing temperature(℃) | PCR延伸时间 Extension time (s) |
---|---|---|---|---|---|---|
1 | Pit | Pit-pF | TTGGAGCTACGGTTGTTCAGA | 655 | 59 | 40 |
Pit-pR | AGTGCTAGAACCTACGGATTCC | |||||
Pit-mF | GAACCAACCGGCAACAAAaT | 682 | 56 | 50 | ||
Pit-mR | CAACTTTGATGGCAAGAGGAAcA | |||||
2 | Pish | Pish/Pi37-F | CCTTGAGAAGCTATGTGTATACAAC | 349 | 55 | 30 |
Pish/Pi35-R | AGAGAGAAACCCTGGAACTTT | |||||
3 | Pib | Pib-F2 | CCATTCCAGCAATCAGGTAGC | 638 | 55 | 40 |
Pib-R2 | AGAGGGCCATACATTTCGCA | |||||
Pib-mF | CTTTCTCTACGAGGATGCTAaC | 205 | 60 | 30 | ||
Pib-mR | GACACCTCTGCATTAAACTAaG | |||||
4 | Pid3 | Pid3-F2 | TCCTTCTGTTGGTGTGCCAG | 692 | 66 | 40 |
Pid3-R2 | TCCGTCGGACTCTATCCACA | |||||
Pid3-mF | AGCTGTGGCAAGCCTAACtC | 166 | 57 | 20 | ||
Pid3-mR | GTCACAAATCATTCGCTCTTTTTaC | |||||
5 | Pi5 | Pi5-1-gF | CAATCCAATATGATAGGACTTAAAA | 496 | 59 | 30 |
Pi5-1-gR | GAAGGAGTTATTCTCCGCAT | |||||
Pi5-1-2F | CATATATCAATTCAGCTGTGCAT | 163 | 59 | 20 | ||
Pi5-1-2R | GGCCCAACTATGGGTATAATAC | |||||
6 | Pia | Pia(RGA5)-F | GGTGGACAGCGTGGAGATAA | 280 | 59 | 20 |
Pia(RGA5)-R | CACATGGTTGAGCAAGGGTT | |||||
7 | Pi54 | Pi54-F | CAAGAATGGCAAAACTTCAAGAG | 314 | 59 | 20 |
Pi54-R | GAGATTATCAACTCCACTTATCTCTG | |||||
Pi54-mF | CAAGAATGGCAAAACTTCAAGtG | 250 | 60 | 20 | ||
Pi54-mR2 | GCCTTTTCACATGCTCTCtT | |||||
8 | Ptr/Pi-ta2 | Ptr/Pi-ta2-F | CAGCAAGGATGCAGATTTGACT | 186 (R) 198 (S) | 59 | 20 |
Ptr/Pi-ta2-R | GGGTATTTCGCAGGCATAAACTC | |||||
Ptr/Pi-ta2-F | CAGCAAGGATGCAGATTTGACT | 154 | 58 | 20 | ||
ptr-Rs | TGGTTTTGGTTYTTCTGGTTTTT |
Table 2. The primers of molecular markers and parameter setting of PCR amplification
序号 No. | R基因 R gene | 引物名称 Primer | 引物序列 Sequence | PCR产物长度 Size of PCR production(bp) | PCR退火温度 Annealing temperature(℃) | PCR延伸时间 Extension time (s) |
---|---|---|---|---|---|---|
1 | Pit | Pit-pF | TTGGAGCTACGGTTGTTCAGA | 655 | 59 | 40 |
Pit-pR | AGTGCTAGAACCTACGGATTCC | |||||
Pit-mF | GAACCAACCGGCAACAAAaT | 682 | 56 | 50 | ||
Pit-mR | CAACTTTGATGGCAAGAGGAAcA | |||||
2 | Pish | Pish/Pi37-F | CCTTGAGAAGCTATGTGTATACAAC | 349 | 55 | 30 |
Pish/Pi35-R | AGAGAGAAACCCTGGAACTTT | |||||
3 | Pib | Pib-F2 | CCATTCCAGCAATCAGGTAGC | 638 | 55 | 40 |
Pib-R2 | AGAGGGCCATACATTTCGCA | |||||
Pib-mF | CTTTCTCTACGAGGATGCTAaC | 205 | 60 | 30 | ||
Pib-mR | GACACCTCTGCATTAAACTAaG | |||||
4 | Pid3 | Pid3-F2 | TCCTTCTGTTGGTGTGCCAG | 692 | 66 | 40 |
Pid3-R2 | TCCGTCGGACTCTATCCACA | |||||
Pid3-mF | AGCTGTGGCAAGCCTAACtC | 166 | 57 | 20 | ||
Pid3-mR | GTCACAAATCATTCGCTCTTTTTaC | |||||
5 | Pi5 | Pi5-1-gF | CAATCCAATATGATAGGACTTAAAA | 496 | 59 | 30 |
Pi5-1-gR | GAAGGAGTTATTCTCCGCAT | |||||
Pi5-1-2F | CATATATCAATTCAGCTGTGCAT | 163 | 59 | 20 | ||
Pi5-1-2R | GGCCCAACTATGGGTATAATAC | |||||
6 | Pia | Pia(RGA5)-F | GGTGGACAGCGTGGAGATAA | 280 | 59 | 20 |
Pia(RGA5)-R | CACATGGTTGAGCAAGGGTT | |||||
7 | Pi54 | Pi54-F | CAAGAATGGCAAAACTTCAAGAG | 314 | 59 | 20 |
Pi54-R | GAGATTATCAACTCCACTTATCTCTG | |||||
Pi54-mF | CAAGAATGGCAAAACTTCAAGtG | 250 | 60 | 20 | ||
Pi54-mR2 | GCCTTTTCACATGCTCTCtT | |||||
8 | Ptr/Pi-ta2 | Ptr/Pi-ta2-F | CAGCAAGGATGCAGATTTGACT | 186 (R) 198 (S) | 59 | 20 |
Ptr/Pi-ta2-R | GGGTATTTCGCAGGCATAAACTC | |||||
Ptr/Pi-ta2-F | CAGCAAGGATGCAGATTTGACT | 154 | 58 | 20 | ||
ptr-Rs | TGGTTTTGGTTYTTCTGGTTTTT |
Fig. 1. Position and application of molecular markers for Pit, Pish or Pib gene A, Gene schematic diagram and the position of molecular markers for Pit gene. LTR, Long terminal repeat (LTR) retrotransposon; B, Primer pair of Pit-pF and Pit-pR was used to detect Pit gene in rice varieties (partial results). M, DNA Marker. C and D, Gene schematic diagram and the position of molecular markers for Pish (C) and Pib (D) gene. The box filled with dots indicates exon. The solid line indicates promoter, intron or 3’ untranslated region while it localizes before initiation codon, between boxes or after stop codon, respectively. E, Three exons of Pib gene in Mianhui 365 were amplified by PCR.
含有Pish基因的材料 Materials containing Pish | 含有Pib基因的材料 Materials containing Pib | 含有Pib基因的材料 Materials containing Pib | 含有Pid3基因的材料 Materials containing Pid3 | 含有Pi5-1基因的材料 Materials containing Pi5-1 |
---|---|---|---|---|
IRBLsh-B | IRBLb-B | 雅恢2275 Yahui 2275 | 德恢6099 Dehui 6099 | IRBL5-M |
R900 | 德恢6099 Dehui 6099 | 雅恢2348 Yahui 2348 | 航恢1179 Hanghui 1179 | E33 |
D35B | 航恢570 Hanghui 570 | 雅恢2918 Yahui 2918 | 华占 Huazhan | 成恢3203 Chenghui 3203 |
G46B | 华占 Huazhan | 岳恢9113 Yuehui 9113 | 蜀恢505 Shuhui 505 | 成恢727 Chenghui 727 |
川谷B Chuangu B | 南恢558 Nanhui 558 | 粤农丝苗 Yuenongsimiao | 蜀恢548 Shuhui 548 | 成恢993 Chenghui 993 |
川华B Chuanhua B | 南恢968 Nanhui 968 | 川106B Chuan 106B | 蜀恢586 Shuhui 586 | 德恢6099 Dehui 6099 |
川农4B Chuannong 4B | 蜀恢505 Shuhui 505 | 川608B Chuan 608B | 五山丝苗 Wushansimiao | 航恢1179 Hanghui 1179 |
德66B De66B | 蜀恢586 Shuhui 586 | 川华B Chuanhua B | 雅禾 Yahe | 航恢570 Hanghui 570 |
广8B Guang 8B | R900 | 川种3B Chuanzhong 3B | 雅恢2115 Yahui 2115 | 华占 Huazhan |
泸香618B Luxiang 618B | 乐恢188 Lehui 188 | 泸香618B Luxiang 618B | 雅恢2116 Yahui 2116 | 绵恢146 Mianhui 146 |
蓉7B Rong 7B | 乐恢584 Lehui 584 | 忠香B Zhongxiang B | 雅恢2118 Yahui 2118 | 南恢558 Nanhui 558 |
武金4B Wujin 4B | 泸恢17 Luhui 17 | 泰丰B Taifeng B | 雅恢2119 Yahui 2119 | 南恢968 Nanhui 968 |
武香B Wuxiang B | 绵恢528 Mianhui 528 | 雅1B Ya 1B | 雅恢2918 Yahui 2918 | 蜀恢257 Shuhui 257 |
雅3B Ya 3B | 蜀恢569 Shuhui 569 | 宜香1B Yixiang 1B | 雅恢5049 Yahui 5049 | 蜀恢308 Shuhui 308 |
雅7B Ya 7B | 蜀恢882 Shuhui 882 | 楚粳28 Chugeng 28 | 雅恢5217 Yahui 5217 | 蜀恢451 Shuhui 451 |
含有Pish基因 | 五山丝苗 Wushansimiao | 野香B Yexiang B | 雨恢38 Yuhui 38 | 蜀恢498 Shuhui 498 |
西科恢768 Xikehui 768 | 绵恢313 Mianhui 313 | 蓉7B Rong 7B | 蜀恢548 Shuhui 548 | |
雅恢2115 Yahui 2115 | 绵恢365 Mianhui 365 | 双1B Shuang 1B | 蜀恢589 Shuhui 589 | |
雅恢2116 Yahui 2116 | 绵恢821 Mianhui 821 | 雅1B Ya 1B | HR2168 | |
雅恢2118 Yahui 2118 | 绵恢823 Mianhui 823 | 绵恢815 Mianhui 815 | R900 | |
雅恢2119 Yahui 2119 | 绵恢815 Mianhui 815 | 乐恢584 Lehui 584 | ||
雅恢2199 Yahui 2199 | 泸恢8258 Luhui 8258 | |||
绵恢523 Mianhui 523 | ||||
绵恢528 Mianhui 528 | ||||
广8B Guang 8B | ||||
绵恢6139 Mianhui 6139 | ||||
绵恢313 Mianhui 313 | ||||
绵恢821 Mianhui 821 | ||||
绵恢837 Mianhui 837 | ||||
绵恢857 Mianhui 857 |
Table 3. Materials containing Pish, Pib, Pid3, and Pi5-1
含有Pish基因的材料 Materials containing Pish | 含有Pib基因的材料 Materials containing Pib | 含有Pib基因的材料 Materials containing Pib | 含有Pid3基因的材料 Materials containing Pid3 | 含有Pi5-1基因的材料 Materials containing Pi5-1 |
---|---|---|---|---|
IRBLsh-B | IRBLb-B | 雅恢2275 Yahui 2275 | 德恢6099 Dehui 6099 | IRBL5-M |
R900 | 德恢6099 Dehui 6099 | 雅恢2348 Yahui 2348 | 航恢1179 Hanghui 1179 | E33 |
D35B | 航恢570 Hanghui 570 | 雅恢2918 Yahui 2918 | 华占 Huazhan | 成恢3203 Chenghui 3203 |
G46B | 华占 Huazhan | 岳恢9113 Yuehui 9113 | 蜀恢505 Shuhui 505 | 成恢727 Chenghui 727 |
川谷B Chuangu B | 南恢558 Nanhui 558 | 粤农丝苗 Yuenongsimiao | 蜀恢548 Shuhui 548 | 成恢993 Chenghui 993 |
川华B Chuanhua B | 南恢968 Nanhui 968 | 川106B Chuan 106B | 蜀恢586 Shuhui 586 | 德恢6099 Dehui 6099 |
川农4B Chuannong 4B | 蜀恢505 Shuhui 505 | 川608B Chuan 608B | 五山丝苗 Wushansimiao | 航恢1179 Hanghui 1179 |
德66B De66B | 蜀恢586 Shuhui 586 | 川华B Chuanhua B | 雅禾 Yahe | 航恢570 Hanghui 570 |
广8B Guang 8B | R900 | 川种3B Chuanzhong 3B | 雅恢2115 Yahui 2115 | 华占 Huazhan |
泸香618B Luxiang 618B | 乐恢188 Lehui 188 | 泸香618B Luxiang 618B | 雅恢2116 Yahui 2116 | 绵恢146 Mianhui 146 |
蓉7B Rong 7B | 乐恢584 Lehui 584 | 忠香B Zhongxiang B | 雅恢2118 Yahui 2118 | 南恢558 Nanhui 558 |
武金4B Wujin 4B | 泸恢17 Luhui 17 | 泰丰B Taifeng B | 雅恢2119 Yahui 2119 | 南恢968 Nanhui 968 |
武香B Wuxiang B | 绵恢528 Mianhui 528 | 雅1B Ya 1B | 雅恢2918 Yahui 2918 | 蜀恢257 Shuhui 257 |
雅3B Ya 3B | 蜀恢569 Shuhui 569 | 宜香1B Yixiang 1B | 雅恢5049 Yahui 5049 | 蜀恢308 Shuhui 308 |
雅7B Ya 7B | 蜀恢882 Shuhui 882 | 楚粳28 Chugeng 28 | 雅恢5217 Yahui 5217 | 蜀恢451 Shuhui 451 |
含有Pish基因 | 五山丝苗 Wushansimiao | 野香B Yexiang B | 雨恢38 Yuhui 38 | 蜀恢498 Shuhui 498 |
西科恢768 Xikehui 768 | 绵恢313 Mianhui 313 | 蓉7B Rong 7B | 蜀恢548 Shuhui 548 | |
雅恢2115 Yahui 2115 | 绵恢365 Mianhui 365 | 双1B Shuang 1B | 蜀恢589 Shuhui 589 | |
雅恢2116 Yahui 2116 | 绵恢821 Mianhui 821 | 雅1B Ya 1B | HR2168 | |
雅恢2118 Yahui 2118 | 绵恢823 Mianhui 823 | 绵恢815 Mianhui 815 | R900 | |
雅恢2119 Yahui 2119 | 绵恢815 Mianhui 815 | 乐恢584 Lehui 584 | ||
雅恢2199 Yahui 2199 | 泸恢8258 Luhui 8258 | |||
绵恢523 Mianhui 523 | ||||
绵恢528 Mianhui 528 | ||||
广8B Guang 8B | ||||
绵恢6139 Mianhui 6139 | ||||
绵恢313 Mianhui 313 | ||||
绵恢821 Mianhui 821 | ||||
绵恢837 Mianhui 837 | ||||
绵恢857 Mianhui 857 |
Fig. 2. Position of molecular markers for Pid3, Pi5-1 or Pia gene A-C, The gene schematic diagram and the position of molecular markers for Pid3 (A), Pi5-1 (B) and Pia (C).
Fig. 3. Position and application of molecular markers for Pi54 or Pita2/Ptr gene A, The gene schematic diagram and the position of molecular markers for Pi54 gene. B, The primer pair of Pi54-mF and Pi54-mR2 was used to detect Pi54 gene in rice varieties (partial results). M: DNA Marker. C, The gene schematic diagram and the position of molecular markers for Pita2/Ptr gene.
Fig. 4. Distribution of eight R genes in rice parent varieties in Sichuan Basin A, Percentage of rice parent varieties which containing the given R gene. B, Percentage of rice parent varieties which containing indicated number of R genes.
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