Chinese Journal OF Rice Science ›› 2019, Vol. 33 ›› Issue (5): 457-466.DOI: 10.16819/j.1001-7216.2019.9036
• Research Papers • Previous Articles Next Articles
Yanhua CHEN1,3, Yaliang WANG1,2,*(), Defeng ZHU1, Qinghua SHI2, Huizhe CHEN1, Jing XIANG1, Yikai ZHANG1, Yuping ZHANG1,*()
Received:
2019-03-29
Revised:
2019-04-15
Online:
2019-09-10
Published:
2019-09-10
Contact:
Yaliang WANG, Yuping ZHANG
陈燕华1,3, 王亚梁1,2,*(), 朱德峰1, 石庆华2, 陈惠哲1, 向镜1, 张义凯1, 张玉屏1,*()
通讯作者:
王亚梁,张玉屏
基金资助:
CLC Number:
Yanhua CHEN, Yaliang WANG, Defeng ZHU, Qinghua SHI, Huizhe CHEN, Jing XIANG, Yikai ZHANG, Yuping ZHANG. Mechanism of Exogenous Brassinolide in Alleviating High Temperature Injury at Panicle Initiation Stage in Rice[J]. Chinese Journal OF Rice Science, 2019, 33(5): 457-466.
陈燕华, 王亚梁, 朱德峰, 石庆华, 陈惠哲, 向镜, 张义凯, 张玉屏. 外源油菜素内酯缓解水稻穗分化期高温伤害的机理研究[J]. 中国水稻科学, 2019, 33(5): 457-466.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2019.9036
基因Gene | 正向引物Forward primer 5' → 3' | 反向引物Reverse primer 5' → 3' |
---|---|---|
OsUBQ | AACCAGCTGAGGCCCAAGA | ACGATTGATTTAACCAGTCCATGA |
OsSUT1 | CCACCTCGGTAGAAGAGAATAA | CCATTCATTACACACTAATTACCAA |
OsSUT2 | AGGAGGAGAGGTCACCGATAA | CCAACATCCAATGTACAACAGCA |
OsSUT4 | TTTGGCTGAGCAGAACACCA | ATGTCATTCGGGCAGAGCTT |
OsLOGL2 | GAGCGCACAGAAAAGAGAAGC | GGCATGAGTGCTTTTGGAAT |
OsLOGL3 | GTGCTGCATTGTCTGCAGTT | GGTCATGAGAGTCTTGGGGA |
OsCKX5 | CGCTGCTGGGCGAGCTGAAT | CGCCTTGTGCACGCGGTCTA |
OsCKX9 | GCCAGGATTCCTCTTGAACCTGC | ACGCACTGGGTCCTGCGGAT |
OsRR2 | ACGATCTTCTCAAAGCCATCAAG | TGAGAGGCTTAAGGATGAAATCCT |
OsRR5 | ACCGAATGTGAGCATGATTATCA | CCTTGACCTTCTTCAGGAGTTCATA |
ORR2 | TGGGTAGTTCAAAGCTGCAG | GACTAGAAAAGGCGCTGACA |
ORR4 | TCAGTGGTGGTCTAGATGAC | CGATGATTAACGAGAATTTTAC |
Table 1 Primers used for quantitative real-time PCR.
基因Gene | 正向引物Forward primer 5' → 3' | 反向引物Reverse primer 5' → 3' |
---|---|---|
OsUBQ | AACCAGCTGAGGCCCAAGA | ACGATTGATTTAACCAGTCCATGA |
OsSUT1 | CCACCTCGGTAGAAGAGAATAA | CCATTCATTACACACTAATTACCAA |
OsSUT2 | AGGAGGAGAGGTCACCGATAA | CCAACATCCAATGTACAACAGCA |
OsSUT4 | TTTGGCTGAGCAGAACACCA | ATGTCATTCGGGCAGAGCTT |
OsLOGL2 | GAGCGCACAGAAAAGAGAAGC | GGCATGAGTGCTTTTGGAAT |
OsLOGL3 | GTGCTGCATTGTCTGCAGTT | GGTCATGAGAGTCTTGGGGA |
OsCKX5 | CGCTGCTGGGCGAGCTGAAT | CGCCTTGTGCACGCGGTCTA |
OsCKX9 | GCCAGGATTCCTCTTGAACCTGC | ACGCACTGGGTCCTGCGGAT |
OsRR2 | ACGATCTTCTCAAAGCCATCAAG | TGAGAGGCTTAAGGATGAAATCCT |
OsRR5 | ACCGAATGTGAGCATGATTATCA | CCTTGACCTTCTTCAGGAGTTCATA |
ORR2 | TGGGTAGTTCAAAGCTGCAG | GACTAGAAAAGGCGCTGACA |
ORR4 | TCAGTGGTGGTCTAGATGAC | CGATGATTAACGAGAATTTTAC |
处理 Treatment | EBR浓度 Concentration of EBR/(mg·L-1) | 单株穗数 Panicle number per plant | 每穗粒数 Number of spikelets per panicle | 结实率 Seed setting rate/% | 千粒重 1000-grain weight/g | 单株产量 Yield per plant /g |
---|---|---|---|---|---|---|
NT | 0.00 | 12.0±1.0 a | 100.7±11.2 a | 81.4±1.8 ab | 19.6±0.3 a | 19.1±0.1 b |
0.05 | 11.3±0.6 a | 105.5±4.4 b | 82.1±1.6 ab | 19.2±1.1 a | 18.8±1.3 b | |
0.10 | 12.3±0.6 a | 111.2±3.4 abc | 86.9±0.2 a | 19.4±1.1 a | 23.1±1.8 a | |
0.15 | 12.7±0.6 a | 121.0±5.2 a | 75.8±5.8 b | 18.6±0.8 a | 21.7±4.9 ab | |
0.30 | 12.3±1.5 a | 113.9±5.2 ab | 79.8±2.8 b | 19.1±0.8 a | 21.3±4.7 ab | |
0.50 | 13.3±1.5 a | 105.8±6.5 bc | 80.0±2.9 b | 19.1±0.7 a | 21.6±2.4 ab | |
HT | 0.00 | 12.3±0.6 a | 52.5±0.9 d | 46.6±7.3 b | 18.0±1.0 a | 5.4±0.5 c |
0.05 | 13.0±2.0 a | 71.0±4.8 bc | 49.8±4.9 b | 17.6±0.3 a | 8.1±1.0 b | |
0.10 | 12.0±1.0 a | 83.2±3.1 a | 45.9±6.8 b | 17.7±0.2 a | 8.1±1.2 b | |
0.15 | 11.7±0.6 a | 85.9±4.2 a | 65.6±9.5 a | 17.7±0.3 a | 11.6±1.3 a | |
0.30 | 11.3±0.6 a | 76.5±1.6 b | 49.4±1.4 b | 18.1±0.5 a | 9.2±0.4 b | |
0.50 | 12.7±0.6 a | 65.7±4.7 c | 55.9±6.9 ab | 18.0±0.7 a | 8.4±1.6 b |
Table 2 Rice yield and its components per plant under exposure to different concentrations of exogenous 2, 4-epibrassinolide (EBR) at high temperature.
处理 Treatment | EBR浓度 Concentration of EBR/(mg·L-1) | 单株穗数 Panicle number per plant | 每穗粒数 Number of spikelets per panicle | 结实率 Seed setting rate/% | 千粒重 1000-grain weight/g | 单株产量 Yield per plant /g |
---|---|---|---|---|---|---|
NT | 0.00 | 12.0±1.0 a | 100.7±11.2 a | 81.4±1.8 ab | 19.6±0.3 a | 19.1±0.1 b |
0.05 | 11.3±0.6 a | 105.5±4.4 b | 82.1±1.6 ab | 19.2±1.1 a | 18.8±1.3 b | |
0.10 | 12.3±0.6 a | 111.2±3.4 abc | 86.9±0.2 a | 19.4±1.1 a | 23.1±1.8 a | |
0.15 | 12.7±0.6 a | 121.0±5.2 a | 75.8±5.8 b | 18.6±0.8 a | 21.7±4.9 ab | |
0.30 | 12.3±1.5 a | 113.9±5.2 ab | 79.8±2.8 b | 19.1±0.8 a | 21.3±4.7 ab | |
0.50 | 13.3±1.5 a | 105.8±6.5 bc | 80.0±2.9 b | 19.1±0.7 a | 21.6±2.4 ab | |
HT | 0.00 | 12.3±0.6 a | 52.5±0.9 d | 46.6±7.3 b | 18.0±1.0 a | 5.4±0.5 c |
0.05 | 13.0±2.0 a | 71.0±4.8 bc | 49.8±4.9 b | 17.6±0.3 a | 8.1±1.0 b | |
0.10 | 12.0±1.0 a | 83.2±3.1 a | 45.9±6.8 b | 17.7±0.2 a | 8.1±1.2 b | |
0.15 | 11.7±0.6 a | 85.9±4.2 a | 65.6±9.5 a | 17.7±0.3 a | 11.6±1.3 a | |
0.30 | 11.3±0.6 a | 76.5±1.6 b | 49.4±1.4 b | 18.1±0.5 a | 9.2±0.4 b | |
0.50 | 12.7±0.6 a | 65.7±4.7 c | 55.9±6.9 ab | 18.0±0.7 a | 8.4±1.6 b |
Fig. 1. Effect of exogenous 2, 4-epibrassinolide (EBR) application(0.15 mg/L) on young panicle development and spikelet formation under high temperature. A, Young panicle under high temperature and normal temperature for 7 days (bar=0.5 cm) and 15 days(bar=1 cm); B, Panicle morphologies at heading stage at high temperature and normal temperature for 15 days(bar=2.5 cm); C, Effect of 0.15 mg/L exogenous EBR on the number of differentiated spikelets; D, Effect of 0.15 mg/L exogenous EBR application on the proportion of degenerated spikelets. Values are Mean±SD; Bars superscripted by different lowercase letters are significantly different at 0.05 level among treatments. The same as below.
Fig. 2. Effect of 0.15 mg/L exogenous 2, 4-epibrassinolide (EBR) application on carbohydrate distribution and transportation of young panicles under high temperature. A, Leaf net photosynthesis; B, Dry matter accumulation of young panicles; C, Non-structural carbohydrate content; D, Relative expression of OsSUT1 of young panicles; E, Relative expression of OsSUT2 of young panicles; F, Relative expression of OsSUT4 of young panicles.
Fig. 3. Effect of 0.15 mg/L exogenous 2, 4-epibrassinalide (EBR) application on enzymes activities related to sucrose utilization under high temperature.
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