QTL Analysis for Mineral Contents in Brown Rice Using a BC2F4:5 Population Derived from Dongxiang Wild Rice (Oryza rufipogon Griff.)

doi:10.16819/j.1001-7216.2018.7011

Abstract

Abstract:

【Objective】 Biofortifying food crops with essential minerals would alleviate mineral deficiencies in humans.【Method】 One plant A58 was selected from Xieqingzao B// Xieqingzao B /Dongxiang wild rice BC₁F₅ population, and was backcrossed with Xieqingzao B to develop a BC₂F_4:5population. The contents of Mg, Ca, Zn, Fe, Mn and Cu in brown rice of 132 BC₂F_4:5lines were measured with an inductively coupled plasma atomic emission spectrometer (ICP-AES). Detection of quantitative trait loci (QTLs) for mineral contents in brown rice was conducted using Windows QTL Cartographer 2.5.【Result】 A total of 17 QTLs for mineral contents in brown rice were detected on chromosomes 1, 4, 6, 8, 9 and 11, respectively, including one for Mg content, four for Ca content, four for Zn content, two for Fe content, two for Mn content, and four for Cu content. The explained phenotypic variations ranged from 5.0% to 47.2%, and eight QTLs of them had the enhancing alleles derived from Oryza rufipogon. 【Conclusion】 Twelve QTLs were clustered in five chromosomal regions, indicating that common genetic-physiological mechanisms were involved for different mineral nutrients, and the beneficial alleles could be utilized to improve grain nutritional quality by marker-assisted selection.

Key words: Dongxiang wild rice, brown rice, mineral content, QTL mapping

摘要：

【目的】强化粮食作物的必需矿物质有利于缓解人们矿质营养缺乏症。【方法】从协青早B//协青早B/东乡野生稻BC₁F₅群体中挑选到1个单株A58,与协青早B回交,构建了BC₂F_4:5群体。采用电感耦合等离子体原子发射光谱仪ICP-AES测定132个BC₂F_4:5株系的糙米Mg、Ca、Zn、Fe、Mn和Cu含量,应用Windows QTL Cartographer 2.5进行糙米矿质QTL分析。【结果】共检测到17个糙米矿质含量QTL,分别位于第1、4、6、8、9和11等6条染色体上,包括Mg含量1个、Ca含量4个、Zn含量4个、Fe含量2个、Mn含量2个和Cu含量4个。这些QTL解释表型变异的5.0%~47.2%,其中8个QTL的增效等位基因来自东乡野生稻。【结论】 12个QTL聚集于5条染色体上的5个QTL簇,表明不同矿质营养元素涉及到共同遗传生理机制,可通过分子标记辅助选择方法将有利等位基因应用于稻米营养品质改良。

关键词: 东乡野生稻, 糙米, 矿质含量, QTL定位

CLC Number:

Biaolin HU, Derun HUANG, Yeqing XIAO, Qiangsheng HE, Yong WAN, Yeyang FAN. QTL Analysis for Mineral Contents in Brown Rice Using a BC₂F_4:5Population Derived from Dongxiang Wild Rice (Oryza rufipogon Griff.)[J]. Chinese Journal OF Rice Science, 2018, 32(1): 43-50.

胡标林, 黄得润, 肖叶青, 何强生, 万勇, 樊叶杨. 应用东乡野生稻回交重组自交系群体分析糙米矿质含量QTL[J]. 中国水稻科学, 2018, 32(1): 43-50.

Figures/Tables 6

References 35

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染色体 Chromosome	标记名称 Name of marker
1	RM10176, RM10300, RM6466, RM129, RM11126
4	RM401, RM273, RM303, RM3474, RM5709, RM349, RM348
5	RM4777
6	RM588, RM190, RM6003, RM19387, RM510, RM3805, RM276, RM19795, RM527, RM3724, RM7311, RM3330, RM564,
	RM19953, RM1161, RM20130, RM20591, RM340
8	RM547, RM22654, RM22684, RM72, RM339, RM42
9	RM1896
11	RM229, RM254, RM21, RM206, RM254, RM1233, RM224
12	RM28597, RM270

染色体 Chromosome	标记名称 Name of marker
1	RM10176, RM10300, RM6466, RM129, RM11126
4	RM401, RM273, RM303, RM3474, RM5709, RM349, RM348
5	RM4777
6	RM588, RM190, RM6003, RM19387, RM510, RM3805, RM276, RM19795, RM527, RM3724, RM7311, RM3330, RM564,
	RM19953, RM1161, RM20130, RM20591, RM340
8	RM547, RM22654, RM22684, RM72, RM339, RM42
9	RM1896
11	RM229, RM254, RM21, RM206, RM254, RM1233, RM224
12	RM28597, RM270

含量 Content	BC₂F_4:5群体 BC₂F_4:5 population						协青早B Xieqingzao B /(mg∙kg^-1)
含量 Content	平均值Mean /(mg∙kg^-1)	标准差 SD	变异系数 CV/%	范围Range /(mg∙kg^-1)	偏度 Skewness	峰度 Kurtosis	协青早B Xieqingzao B /(mg∙kg^-1)
Mg	1384.00	90.56	6.54	1207~1637	0.61	0.17	1256.00
Ca	148.20	18.28	12.33	115.2~201.4	0.54	–0.35	134.70
Zn	26.90	2.07	7.70	22.66~32.06	0.27	–0.46	26.71
Fe	12.64	1.11	8.78	10.70~16.13	1.46	3.87	12.38
Mn	43.11	3.93	9.12	35.61~53.56	0.33	–0.55	38.21
Cu	3.19	0.26	8.15	2.64~3.87	0.14	–0.44	2.77

含量 Content	BC₂F_4:5群体 BC₂F_4:5 population						协青早B Xieqingzao B /(mg∙kg^-1)
含量 Content	平均值Mean /(mg∙kg^-1)	标准差 SD	变异系数 CV/%	范围Range /(mg∙kg^-1)	偏度 Skewness	峰度 Kurtosis	协青早B Xieqingzao B /(mg∙kg^-1)
Mg	1384.00	90.56	6.54	1207~1637	0.61	0.17	1256.00
Ca	148.20	18.28	12.33	115.2~201.4	0.54	–0.35	134.70
Zn	26.90	2.07	7.70	22.66~32.06	0.27	–0.46	26.71
Fe	12.64	1.11	8.78	10.70~16.13	1.46	3.87	12.38
Mn	43.11	3.93	9.12	35.61~53.56	0.33	–0.55	38.21
Cu	3.19	0.26	8.15	2.64~3.87	0.14	–0.44	2.77

性状Trait	Mg	Ca	Zn	Fe	Mn
Ca	0.288^**
Zn	0.362^**	–0.057
Fe	0.343^**	0.241^**	0.263^**
Mn	0.514^**	0.568^**	0.387^**	0.048
Cu	0.186^*	–0.212^*	0.188^*	–0.013	0.164

QTL Analysis for Mineral Contents in Brown Rice Using a BC₂F_4:5Population Derived from Dongxiang Wild Rice (Oryza rufipogon Griff.)

应用东乡野生稻回交重组自交系群体分析糙米矿质含量QTL

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Figures/Tables 6

References 35

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Metrics

性状 Trait	QTL	区间 Interval	LOD值 LOD value	加性效应^a Additive effect^a	显性效应 Dominance effect	显性度 Dominance degree	贡献率 Proportion of the variance explained/%	文献 Reference
Mg	qMg1	RM10176-RM10300	4.86	–41.76	–18.81	–0.45	15.5	20
Ca	qCa1	RM10176-RM10300	8.73	–9.00	–4.36	–0.48	15.1
	qCa4	RM273-RM303	3.12	17.88	–4.98	–0.28	13.6	20
	qCa6	RM588-RM204	9.62	10.11	9.66	0.96	17.2	18
	qCa11	RM21-RM206	7.27	–5.17	10.56	2.04	13.5
Zn	qZn4	RM3474-RM5709	11.01	0.99	0.06	0.06	16.1	9, 11
	qZn6.1	RM588-RM204	3.77	–0.66	–0.24	–0.37	5.0	9, 11, 14, 15, 18
	qZn6.2	RM20591-RM340	6.10	–0.92	0.21	0.22	8.3	13
	qZn8	RM72-RM22654	7.48	–0.97	–0.02	–0.02	11.2	10, 15
Fe	qFe1	RM140-RM129	3.66	1.31	0.82	0.63	38.8	19
	qFe11	RM206-RM254	3.73	0.25	0.99	3.93	23.1
Mn	qMn6	RM7311-RM3330	3.24	–0.49	2.06	4.18	5.8
	qMn11	RM21-RM206	18.58	–2.81	1.90	0.68	47.2	9
Cu	qCu6.1	RM588-RM204	3.85	–0.11	0.34	3.14	10.7	15, 18, 20
	qCu6.2	RM3724-RM7311	6.08	0.05	0.20	3.66	16.6
	qCu8	RM547-RM72	7.40	0.14	–0.18	–1.29	21.9	15
	qCu9	RM1896-RM566	3.59	0.09	0.08	0.86	9.6	21

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