Difference of Metabolites in Brown Rice Between Liaojing 433 and Koshihikari with Good Eating Quality

doi:10.16819/j.1001-7216.2020.9116

Abstract

Abstract:

【Objective】Our goal is to analyze the metabolite difference between good eating-quality japonica rice Liaojing 433 and Koshihikari, and provide reference for breeding good eating-quality rice. 【Method】 GC-MS was used to detect metabolites in brown rice of Liaojing 433 and Koshihikari. Online software was used to analyze metabolic spectrum and metabolic pathway differences. 【Result】 233 metabolites were identified in both cultivars by GC-MS, among which 158 in Liaojing 433 have higher contents than in Koshihikari, accounting for 67.81% of the total. PCA and PLS-DA analysis found that there were different metabolic profiles between Liaojing 433 and Koshihikari with significant differences in contents of 64 metabolites. Among different metabolites, 42 metabolites had relative high contents in Liaojing 433. Glucosamine acid, trisaccharide, cellobiose, 4-aminobutyric acid, oleic acid, α-tocopherol, pantothenic acid, inositol, quinic acid and chlorogenic acid of Liaojing 433 were 23.86, 13.35, 2.53, 2.44, 9.32, 2.66, 2.90, 3.54, 4.05 and 2.10 times as many as those in Koshihikari, respectively. There are 22 kinds of relative high contents in brown rice in Koshihikari, and the nutrition-related arabinol, linoleic acid, ferulic acid and inositol-4-phosphoric acid are 1.61, 2.33, 1.39 and 2.39 times as many of those in Liaojing 433. Between Liaojing 433 and Koshihikari, there were significant differences in 11 metabolic pathways. The relative contents of metabolites detected by inositol phosphate metabolism, ascorbic acid and aldehyde acid metabolism, tyrosine metabolism and phenylpropane biosynthesis of Liaojing 433 were all higher than those of Koshihikari. 【Conclusion】 The metabolite composition of Liaojing 433 and Koshihikari brown rice was significantly different. Compared with Koshihikari, Liaojing 433 has a good taste and high dietary fiber content. In addition, Liaojing 433 has stronger health care function and antioxidant capacity. GC-MS metabolomics analysis method is a powerful tool for analyzing nutritional differences among rice varieties.

Key words: good eating quality, brown rice, metabolomics, nutritional quality

摘要：

【目的】分析优质食味粳稻辽粳433和越光之间代谢产物差异,为优质食味粳稻辽粳433推广及优质食味粳稻品种选育提供参考。【方法】利用气相色谱-质谱(GC-MS)检测辽粳433和越光的糙米代谢产物,采用在线软件对两个品种糙米差异代谢产物和差异代谢通路进行分析。【结果】通过GC-MS检测两个品种均定性到233种代谢产物。与越光相比,辽粳433糙米代谢产物相对含量高的有158种,占检测到的代谢物总数的67.81%。PCA和PLS-DA分析可以将辽粳433和越光糙米代谢产物分离开。辽粳433与越光糙米之间共有64种代谢产物含量差异显著,辽粳433糙米中相对含量高的差异代谢产物有42种,与营养相关的氨基葡萄糖酸、三糖、纤维二糖、4-氨基丁酸、油酸、α-生育酚、泛酸、肌醇、奎尼酸和绿原酸分别是越光的23.86、13.35、2.53、2.44、9.32、2.66、2.90、3.54、4.05和2.10倍;越光糙米中相对含量高的差异代谢产物有22种,与营养相关的阿拉伯糖醇、亚油酸、阿魏酸和肌醇-4-磷酸是辽粳433的1.61、2.33、1.39和2.39倍。辽粳433与越光糙米之间11个代谢通路有显著差异,肌醇磷酸代谢、抗坏血酸和醛酸代谢、酪氨酸代谢和苯丙烷生物合成检测到的代谢产物相对含量均表现为辽粳433高于越光。【结论】辽粳433与越光糙米之间代谢产物组成差异显著。与越光相比,辽粳433具有与营养相关的差异代谢产物数量更多,含量更高。辽粳433具有成为高膳食纤维和保健功能大米的潜力。GC-MS代谢组学分析方法可作为水稻品种间营养差异分析的有力工具。

关键词: 优质食味, 糙米, 代谢组学, 营养品质

Feng SHEN, Hongbo JIANG, Bo LIU, Xiuru ZHANG, Jun LIU, Wenxiao XIE, Jipan YAO, Liang MA. Difference of Metabolites in Brown Rice Between Liaojing 433 and Koshihikari with Good Eating Quality[J]. Chinese Journal OF Rice Science, 2020, 34(4): 359-367.

沈枫, 蒋洪波, 刘博, 张秀茹, 刘军, 解文孝, 姚继攀, 马亮. 优质食味粳稻辽粳433和越光糙米代谢产物差异分析[J]. 中国水稻科学, 2020, 34(4): 359-367.

Figures/Tables 5

References 48

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序号 No.	代谢产物 Metabolites	VIP	P	序号 No.	代谢产物 Metabolites	VIP	P
1	儿茶素 Catechin	1.4364		33	奎宁酸 Quinic acid	1.4048
2	苯乙酰胺 Phenylacetamide	2.0567		34	4-氨基丁酸 4-aminobutyric acid	1.1059
3	N-乙酰-D-己糖胺	1.1147		35	油酸 Oleic acid	1.0250
	N-acetyl-D-hexosamine			36	海藻糖-6-磷酸 Trehalose-6-phosphate	1.1060
4	正辛醛 Octanal	3.5360		37	3-羟基-3-4'-羟基-3'-甲氧基苯丙酸	1.6832	3.7968
5	核糖-5-磷酸 Ribose-5-phosphate	1.2869			3-hydroxy-3-4'-hydroxy-3'-methoxyphe
6	3-羟基苯甲酸 3-hydroxybenzoic acid	1.2889	3.5573		nylpropionic acid
7	肌醇-4-单磷酸	1.5187	5.4579	38	肌酐 Creatinine	2.3204	4.9027
	Inositol-4-monophosphate			39	鸟苷 Guanosine	1.1265	3.3626
8	甘油 Glycerol		2.1451	40	帕拉金糖醇 Palatinitol	1.5140	3.8164
9	尿酸 Uric acid		5.8882	41	双半乳糖醛酸 Digalacturonic acid	3.9823	2.5444
10	延胡索酸 Fumaric acid		3.3667	42	氨基葡萄糖酸 Glucosaminic acid	4.4534	4.4615
11	半乳糖醛酸 Galacturonic acid		3.0153	43	2'-脱氧鸟苷 2'-deoxyguanosine	6.3652	9.7448
12	β-甘油磷酸 Beta-glycerolphosphate	1.0675	3.3689	44	胱硫醚 Cystathionine	2.1555	7.5433
13	磷酸甘油酯 Glycerol-α-phosphate	1.2960	2.4937	45	1, 5-脱水葡萄糖醇 1, 5-anhydroglucitol		2.9614
14	阿魏酸 Ferulic acid		3.4483	46	5-羟基-3-吲哚乙酸	1.3300	4.1545
15	乳糖酸 Lactobionic acid		2.5625		5-hydroxy-3-indoleacetic acid
16	1-甲基海因 1-methylhydantoin	3.7246	4.2807	47	6-脱氧葡萄糖 6-Deoxyglucose	1.0534	5.8862
17	赖氨酸 Lysine	1.1595	3.5415	48	肌醇 Myo-inositol	1.1812	6.4424
18	2-酮葡萄糖二甲基缩醛	1.7079		49	泛酸 Pantothenic acid	1.5303	5.4521
	2-ketoglucose dimethylacetal			50	N-乙酰半乳糖胺 N-acetylgalactosamine		4.0490
19	己内酰胺 Epsilon-caprolactam	2.6232	2.3184	51	四氧酸 Alloxanoic acid	1.2308	3.9297
20	阿拉伯糖醇 Arabitol	1.1096		52	绿原酸 Chlorogenic acid	1.0311	3.9339
21	胞嘧啶 Cytosin	2.1537		53	瓜氨酸 Citrulline	1.2555
22	亚油酸 Linoleic acid	1.1261		54	鸟氨酸 Ornithine	1.4025
23	琥珀酸 Succinic acid	1.0970		55	胍丁胺 Agmatine		2.2319
24	木糖 Xylulose	1.0928		56	α-生育酚 Alpha tocopherol	2.5750
25	二十二碳烯酸 Docosenoic acid	1.2319		57	β-丙氨酸 Beta-alanine	1.2123	2.3480
26	1, 2, 3, 4, 5, 6, 6氘代-葡萄糖	1.1868		58	纤维二糖 Cellobiose	1.4162	2.9658
	Glucose-1, 2, 3, 4, 5, 6, 6 deuterated			59	核糖醇 Ribitol		2.7145
27	二十八酸 Montanic acid	1.5310		60	2-(哌啶基)苯甲腈		2.3812
28	半乳酸 Galactonic acid	1.1788	2.3643		2-piperidinobenzonitrile
29	乳酸 Lactic acid	1.1132		61	5-单磷酸胞苷 Cytidine-5-monophosphate		3.6268
30	1, 2, 4-苯三酚 1, 2, 4-benzenetriol	1.7583		62	β-甘露醇甘油酯 Beta-mannosylglycerate		3.1918
31	三糖 Trisaccharide	2.3646		63	5-甲氧色胺 5-methoxytryptamine		2.1717
32	肌苷 Inosine	1.2861		64	腐胺 Putrescine		2.1706

序号 No.	代谢产物 Metabolites	VIP	P	序号 No.	代谢产物 Metabolites	VIP	P
1	儿茶素 Catechin	1.4364		33	奎宁酸 Quinic acid	1.4048
2	苯乙酰胺 Phenylacetamide	2.0567		34	4-氨基丁酸 4-aminobutyric acid	1.1059
3	N-乙酰-D-己糖胺	1.1147		35	油酸 Oleic acid	1.0250
	N-acetyl-D-hexosamine			36	海藻糖-6-磷酸 Trehalose-6-phosphate	1.1060
4	正辛醛 Octanal	3.5360		37	3-羟基-3-4'-羟基-3'-甲氧基苯丙酸	1.6832	3.7968
5	核糖-5-磷酸 Ribose-5-phosphate	1.2869			3-hydroxy-3-4'-hydroxy-3'-methoxyphe
6	3-羟基苯甲酸 3-hydroxybenzoic acid	1.2889	3.5573		nylpropionic acid
7	肌醇-4-单磷酸	1.5187	5.4579	38	肌酐 Creatinine	2.3204	4.9027
	Inositol-4-monophosphate			39	鸟苷 Guanosine	1.1265	3.3626
8	甘油 Glycerol		2.1451	40	帕拉金糖醇 Palatinitol	1.5140	3.8164
9	尿酸 Uric acid		5.8882	41	双半乳糖醛酸 Digalacturonic acid	3.9823	2.5444
10	延胡索酸 Fumaric acid		3.3667	42	氨基葡萄糖酸 Glucosaminic acid	4.4534	4.4615
11	半乳糖醛酸 Galacturonic acid		3.0153	43	2'-脱氧鸟苷 2'-deoxyguanosine	6.3652	9.7448
12	β-甘油磷酸 Beta-glycerolphosphate	1.0675	3.3689	44	胱硫醚 Cystathionine	2.1555	7.5433
13	磷酸甘油酯 Glycerol-α-phosphate	1.2960	2.4937	45	1, 5-脱水葡萄糖醇 1, 5-anhydroglucitol		2.9614
14	阿魏酸 Ferulic acid		3.4483	46	5-羟基-3-吲哚乙酸	1.3300	4.1545
15	乳糖酸 Lactobionic acid		2.5625		5-hydroxy-3-indoleacetic acid
16	1-甲基海因 1-methylhydantoin	3.7246	4.2807	47	6-脱氧葡萄糖 6-Deoxyglucose	1.0534	5.8862
17	赖氨酸 Lysine	1.1595	3.5415	48	肌醇 Myo-inositol	1.1812	6.4424
18	2-酮葡萄糖二甲基缩醛	1.7079		49	泛酸 Pantothenic acid	1.5303	5.4521
	2-ketoglucose dimethylacetal			50	N-乙酰半乳糖胺 N-acetylgalactosamine		4.0490
19	己内酰胺 Epsilon-caprolactam	2.6232	2.3184	51	四氧酸 Alloxanoic acid	1.2308	3.9297
20	阿拉伯糖醇 Arabitol	1.1096		52	绿原酸 Chlorogenic acid	1.0311	3.9339
21	胞嘧啶 Cytosin	2.1537		53	瓜氨酸 Citrulline	1.2555
22	亚油酸 Linoleic acid	1.1261		54	鸟氨酸 Ornithine	1.4025
23	琥珀酸 Succinic acid	1.0970		55	胍丁胺 Agmatine		2.2319
24	木糖 Xylulose	1.0928		56	α-生育酚 Alpha tocopherol	2.5750
25	二十二碳烯酸 Docosenoic acid	1.2319		57	β-丙氨酸 Beta-alanine	1.2123	2.3480
26	1, 2, 3, 4, 5, 6, 6氘代-葡萄糖	1.1868		58	纤维二糖 Cellobiose	1.4162	2.9658
	Glucose-1, 2, 3, 4, 5, 6, 6 deuterated			59	核糖醇 Ribitol		2.7145
27	二十八酸 Montanic acid	1.5310		60	2-(哌啶基)苯甲腈		2.3812
28	半乳酸 Galactonic acid	1.1788	2.3643		2-piperidinobenzonitrile
29	乳酸 Lactic acid	1.1132		61	5-单磷酸胞苷 Cytidine-5-monophosphate		3.6268
30	1, 2, 4-苯三酚 1, 2, 4-benzenetriol	1.7583		62	β-甘露醇甘油酯 Beta-mannosylglycerate		3.1918
31	三糖 Trisaccharide	2.3646		63	5-甲氧色胺 5-methoxytryptamine		2.1717
32	肌苷 Inosine	1.2861		64	腐胺 Putrescine		2.1706

差异代谢途径 Pathway name	检测到的代谢物种类/参与此途径的代谢物总数 Detected/Total	检测到的代谢产物种类Types of metabolites detected
差异代谢途径 Pathway name	检测到的代谢物种类/参与此途径的代谢物总数 Detected/Total	辽粳433中相对含量高的代谢产物 Metabolites with relatively high contents in Liaojing 433	越光中相对含量高的代谢产物 Metabolites with relatively high contents in Koshihikari
肌醇磷酸代谢Inositol phosphate metabolism	1/17	肌醇 Myo-inositol
抗坏血酸和醛酸代谢 Ascorbate and aldarate metabolism	3/14	肌醇、抗坏血酸、尿苷二磷酸葡萄糖醛酸 Myo-inositol, ascorbic acid, uridine diphosphate glucuronic acid
半胱氨酸和蛋氨酸代谢 Cysteine and methionine metabolism	7/35	胱硫醚、丝氨酸、半胱氨酸、高丝氨酸、天冬氨酸、丙酮酸 Cystathionine, serine, cysteine, homoserine, aspartic acid, pyruvic acid		甲硫氨酸 Methionine
赖氨酸生物合成 Lysine biosynthesis	4/9	二氨基丙酸、天冬氨酸、高丝氨酸 Diaminopimelic acid, aspartic acid, homoserine		赖氨酸 Lysine
泛酸盐和辅酶A生物合成 Pantothenate and CoA biosynthesis	5/16	β-丙氨酸、丙酮酸、泛酸 Beta-alanine, pyruvic acid, pantothenic acid		缬氨酸、尿嘧啶Valine, uracil
β-丙氨酸代谢 beta-alanine metabolism	5/12	β-丙氨酸、天冬氨酸泛酸、亚精胺 Beta-alanine, aspartic acid, pantothenic acid, spermidine		尿嘧啶 Uracil
嘌呤代谢 Purine metabolism	10/55	谷氨酰胺、次黄嘌呤、硫酸盐、腺嘌呤、肌苷、鸟苷、尿囊酸、脱氧鸟苷 Glutamine, hypoxanthine sulfate, adenine, inosine, guanosine, allantoic acid, deoxyguanosine		核酮糖-5-磷酸、尿酸Ribulose-5-phosphate, uric acid
苯丙烷生物合成 Phenylpropanoid biosynthesis	5/31	酪氨酸、苯丙氨酸、4-羟基肉桂酸、阿魏酸、芥酸钠Tyrosine, phenylalanine, 4-hydroxycinnamic acid, ferulate, sinapate
酪氨酸代谢 Tyrosine metabolism	4/18	多巴、酪氨酸、琥珀酸、延胡索酸 Dopa, tyrosine, fumaric acid, succinic acid
色氨酸代谢 Tryptophan metabolism	4/25	羟色胺、吲哚乙酸、5-羟基吲哚乙酸 Serotonin, indoleacetic acid, 5-hydroxyindoleacetic acid		色氨酸 Tryptophan
亚油酸代谢Linoleic acid metabolism	1/5			亚油酸 Linoleic acid

差异代谢途径 Pathway name	检测到的代谢物种类/参与此途径的代谢物总数 Detected/Total	检测到的代谢产物种类Types of metabolites detected
差异代谢途径 Pathway name	检测到的代谢物种类/参与此途径的代谢物总数 Detected/Total	辽粳433中相对含量高的代谢产物 Metabolites with relatively high contents in Liaojing 433	越光中相对含量高的代谢产物 Metabolites with relatively high contents in Koshihikari
肌醇磷酸代谢Inositol phosphate metabolism	1/17	肌醇 Myo-inositol
抗坏血酸和醛酸代谢 Ascorbate and aldarate metabolism	3/14	肌醇、抗坏血酸、尿苷二磷酸葡萄糖醛酸 Myo-inositol, ascorbic acid, uridine diphosphate glucuronic acid
半胱氨酸和蛋氨酸代谢 Cysteine and methionine metabolism	7/35	胱硫醚、丝氨酸、半胱氨酸、高丝氨酸、天冬氨酸、丙酮酸 Cystathionine, serine, cysteine, homoserine, aspartic acid, pyruvic acid		甲硫氨酸 Methionine
赖氨酸生物合成 Lysine biosynthesis	4/9	二氨基丙酸、天冬氨酸、高丝氨酸 Diaminopimelic acid, aspartic acid, homoserine		赖氨酸 Lysine
泛酸盐和辅酶A生物合成 Pantothenate and CoA biosynthesis	5/16	β-丙氨酸、丙酮酸、泛酸 Beta-alanine, pyruvic acid, pantothenic acid		缬氨酸、尿嘧啶Valine, uracil
β-丙氨酸代谢 beta-alanine metabolism	5/12	β-丙氨酸、天冬氨酸泛酸、亚精胺 Beta-alanine, aspartic acid, pantothenic acid, spermidine		尿嘧啶 Uracil
嘌呤代谢 Purine metabolism	10/55	谷氨酰胺、次黄嘌呤、硫酸盐、腺嘌呤、肌苷、鸟苷、尿囊酸、脱氧鸟苷 Glutamine, hypoxanthine sulfate, adenine, inosine, guanosine, allantoic acid, deoxyguanosine		核酮糖-5-磷酸、尿酸Ribulose-5-phosphate, uric acid
苯丙烷生物合成 Phenylpropanoid biosynthesis	5/31	酪氨酸、苯丙氨酸、4-羟基肉桂酸、阿魏酸、芥酸钠Tyrosine, phenylalanine, 4-hydroxycinnamic acid, ferulate, sinapate
酪氨酸代谢 Tyrosine metabolism	4/18	多巴、酪氨酸、琥珀酸、延胡索酸 Dopa, tyrosine, fumaric acid, succinic acid
色氨酸代谢 Tryptophan metabolism	4/25	羟色胺、吲哚乙酸、5-羟基吲哚乙酸 Serotonin, indoleacetic acid, 5-hydroxyindoleacetic acid		色氨酸 Tryptophan
亚油酸代谢Linoleic acid metabolism	1/5			亚油酸 Linoleic acid