中国水稻科学
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  2011, Vol. 25 Issue (4): 349-356    DOI: 10.3969/j.issn.1001-7216.2011.04.002
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Responses of  Rice Plants of Wuyunjing 7 to Nitrate as Affected by  OverExpression of OsNRT1.2
MA  Cui, FAN  Xiao-Rong-*, XU  Guo-Hua
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
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Abstract A  putative lowaffinity nitrate transporter, OsNRT1.2   gene, was identified based  on the rice genomic database and bioinformatics analysis. By Agrobacterium tumefaciens  transformation with a Ubiquitin (Ubi) promoter, the gain of function of rice OsNRT1.2  was tested by overexpression in Wuyunjing 7. The OsNRT1.2  cDNA from Knowledgebased Oryza Molecular Biological Encyclopedia (KOME) database encoded 533 amino acids with a fulllength of 2178 bp and an open reading  fragment (ORF) of 1732 bp. The expression  pattern of OsNRT1.2  in Wuyunjing 7 was investigated and the results showed that its mRNA was only expressed in roots but not in shoots.  And the overexpressed  plants were generated by the transformation of pUbiOsNRT1.2  into Wuyunjing 7. Wild type (WT) and three T2 generation transgenic lines, named as OE1, OE2 and OE8, were used in the growth experiment at 0.2 and 5.0  mmol/L  nitrate  for 30 days and the plant height and root length were recorded every 10 days. The results showed that the plant height  of  WT and transgenic plants had no difference except OE8 line at 5.0 mmol/L  NO3-  , while the root length of transgenic lines significantly  decreased at both 0.2   and 5.0 mmol/L  nitrate. Nitrogen (N) content in shoots  and roots  was not different between WT and transgenic lines at 0.2 mmol/L  NO3-. But at 5.0  mmol/L  NO3-, compared with WT, the shoot nitrogen content of OE2 and OE8 except OE1 was increased and the root N content of all transgenic lines was significantly decreased.  The rootshoot ratio of transgenic lines was significantly decreased at 0.2 and 5.0 mmol/L nitrate levels. The dry weight of transgenic lines was significantly increased by from 9.4% to 31.1% at 0.2 mmol/L  nitrate and from 12.5% to 43.8% at 5.0 mmol/L  nitrate. It indicated that OsNRT1.2  in Wuyunjing 7 might  be involved in the N translocation from plant roots to shoots and therefore shoot biomass was increased.
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Articles by authors
MA Cui
FAN Xiao-Rong-*
XU Guo-Hua
Key wordsrice   lowaffinity nitrate transporter gene   overexpression   nitrogen    translocation     
Received: 2010-12-01;
Cite this article:   
MA Cui,FAN Xiao-Rong-*,XU Guo-Hua. Responses of  Rice Plants of Wuyunjing 7 to Nitrate as Affected by  OverExpression of OsNRT1.2[J]. , 2011, 25(4): 349-356.
 
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Effects of Long-Term Rice-Rice-Green Manure Rotation on Rice Yield

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Resistance Performance of Rice Varieties (Combinations) to Brown Planthopper,Nilaparvata lugens, in Paddy Field

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