Model-Based Analysis on the Pollen Viabilities of Transgenic Rice B2 at Different Temperatures

doi:10.3969/j.issn.1001-7216.2011.02.016

Abstract

Abstract: The pollen viability of a genetically-modified japonica rice, B2, containing bar gene with herbicide Basta resistance was investigated under nine temperatures(5℃, 10℃, 15℃, 20℃, 25℃, 30℃, 35℃, 40℃, and 45℃) by 2,3,5-triphenyl tetrazolium chloride (TTC) method in vitro after 0, 1, 2, 3, 4, 5, 6, 8, 12, and 14 min from anther. The result showed that the pollen viability decreased with the passing time in vitro, like as “S curve”, which could be accurately described by the Logistic equation. In vitro pollen viability first increased and then decreased with the rising temperature. The optimal temperature for pollen viability ranged from 20℃ to 30℃, and 25℃ was the most optimum temperature. High temperature stress on the pollen viability was more obvious than low temperature. Two equations, an exponential one and a Logistic one, could give the satisfied predicts of the pollen viability duration and pollen longevity, helping to estimate the risk of transgenic flow.

Key words: transgenic rice, pollen viability, pollen longevity, temperature

摘要： 以含bar基因的抗Basta转基因粳稻B2为材料，分别在5℃、10℃、15℃、20℃、25℃、30℃、35℃、40℃、45℃下进行处理，用2,3,5-氯化三苯基四氮唑（TTC）染色法测定了不同温度处理下转基因水稻B2花粉离体0、1、2、3、4、5、6、8、12、14 min后的活力。B2花粉离体后活力呈现“S”型下降，用Logistic模型可精确模拟两者的关系; 其次, B2花粉离体后活力随着温度先升后降，花粉活力的最适温度范围是20℃~30℃，25℃时活力最强，高温对花粉活力的胁迫作用更大。采用指数方程和Logistic方程分段函数，建立B2花粉活力的温度模型，可精确预测花粉存活时间和寿命，为进一步准确估算转基因水稻基因飘移距离提供依据。

关键词: 转基因水稻, 花粉活力, 花粉寿命, 温度, 模拟

LIU Shou-dong,SHI Pei-jian,JIANG Xiao-dong,YAO Ke-min,HU Ning*. Model-Based Analysis on the Pollen Viabilities of Transgenic Rice B2
at Different Temperatures[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2011.02.016 .

刘寿东, 史佩剑, 江晓东, 姚克敏, 胡凝. 转基因水稻B2花粉活力的温度模型[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2011.02.016 .

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Model-Based Analysis on the Pollen Viabilities of Transgenic Rice B2
at Different Temperatures

转基因水稻B2花粉活力的温度模型

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