Development and Application of KASP Marker for Broad-Spectrum Resistance Gene PigmR to Rice Blast

doi:10.16819/j.1001-7216.2025.240104

Chinese Journal OF Rice Science ›› 2025, Vol. 39 ›› Issue (3): 365-372.DOI: 10.16819/j.1001-7216.2025.240104

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Development and Application of KASP Marker for Broad-Spectrum Resistance Gene PigmR to Rice Blast

LI Wenqi¹^,²^,³, XU Yang¹^,²^,³, WANG Fangquan¹^,²^,³, ZHU Jianping¹^,²^,³, TAO Yajun¹^,²^,³, LI Xia¹^,²^,³, FAN Fangjun¹^,²^,³, JIANG Yanjie¹^,²^,³, CHEN Zhihui¹^,²^,³, YANG Jie¹^,²^,³^,^*()

¹Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River (Nanjing), Ministry of Agricultural and Rural Affairs/ Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China
²Zhongshan Biological Breeding Laboratory, Nanjing 210014, China
³Jiangsu Co-innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China

Received:2024-09-08 Revised:2025-02-25 Online:2025-05-10 Published:2025-05-21
Contact: *email: yangjie168@aliyun.com

广谱抗稻瘟病基因PigmR的KASP标记开发及应用

李文奇¹^,²^,³, 许扬¹^,²^,³, 王芳权¹^,²^,³, 朱建平¹^,²^,³, 陶亚军¹^,²^,³, 李霞¹^,²^,³, 范方军¹^,²^,³, 蒋彦婕¹^,²^,³, 陈智慧¹^,²^,³, 杨杰¹^,²^,³^,^*()

¹江苏省农业科学院粮食作物研究所/农业农村部淮河下游种质创制重点实验室（南京）/国家水稻改良中心南京分中心，南京 210014
²生物育种钟山实验室，南京 210014
³扬州大学江苏省粮食作物现代产业技术协同创新中心，江苏扬州 225009

通讯作者: *email: yangjie168@aliyun.com
基金资助:
江苏省种业振兴揭榜挂帅项目[JBGS（2021）039];江苏省重点研发计划（现代农业）资助项目(BE2021374);国家自然科学基金面上项目(42071023);生物育种钟山实验室项目(BM2022008-03);生物育种钟山实验室项目(1201);生物育种钟山实验室项目(1002);江苏省自然科学基金资助项目(BK20212010)

1. .doc(129KB)

Abstract

Abstract:

【Objective】The broad-spectrum resistance gene PigmR has important breeding value for the genetic improvement of rice blast resistance. Developing kompetitive allele specific PCR (KASP) markers based on its coding region variation sites is beneficial for improving the accuracy and efficiency of PigmR utilization in breeding.【Method】This study identified single nucleotide variations in the coding region of PigmR through sequence alignment, developed the KASP molecular marker PigmR-K, conducted genotype analysis using rice varieties and F₂ segregating populations, combined these results with rice blast symptom identification, and carried out molecular marker assisted selection breeding to verify the precision and efficiency of this marker.【Result】Based on an A-to-C mutation at position 2503 in the PigmR gene coding region sequence, a precise and efficient PigmR genotyping technology system based on KASP markers was established. The genotyping of 159 rice varieties, 270 F₂ individuals, and 247 BC₁F₃population lines demonstrated that PigmR-K reliably distinguished the homozygous resistant, homozygous susceptible and heterozygous genotypes of PigmR. These results were completely consistent with disease symptoms from artificial panicle blast inoculation.【Conclusion】The KASP marker PigmR-K developed based on the variation site of on the coding region has advantages such as precision and efficiency, which is beneficial for genetic improvement of rice blast resistance.

Key words: rice, broad-spectrum resistance, rice blast, PigmR gene, KASP, breeding efficiency

摘要：

【目的】广谱抗稻瘟病基因PigmR对水稻抗稻瘟病遗传改良具有重要育种价值。根据其编码区变异位点开发竞争性等位基因特异性PCR(Kompetitive allele specific，PCR KASP)标记，有利于提升PigmR育种利用的效率。【方法】通过序列比对明确PigmR基因编码区存在单核苷酸变异，开发KASP分子标记PigmR-K，通过对水稻品种和F₂分离群体进行基因型分析，结合稻瘟病表型鉴定，并开展分子标记辅助选择育种，对标记的精准性和高效性进行验证。【结果】根据PigmR基因编码区序列第2503位存在特异性单核苷酸A>C变异，建立基于KASP的PigmR精准高效基因分型技术体系，通过对159份水稻品种、270份F₂群体单株以及247份BC₁F₃群体单株进行基因型检测，PigmR-K能够高效区分供试水稻材料的纯合抗病、纯合感病和杂合基因型，并与人工接种穗颈瘟症状一致。【结论】利用PigmR基因编码区变异位点开发的KASP标记PigmR-K具有精准高效等优势，有利于稻瘟病抗性遗传改良。

关键词: 水稻, 广谱抗性, 稻瘟病, PigmR基因, KASP, 育种效率

LI Wenqi, XU Yang, WANG Fangquan, ZHU Jianping, TAO Yajun, LI Xia, FAN Fangjun, JIANG Yanjie, CHEN Zhihui, YANG Jie. Development and Application of KASP Marker for Broad-Spectrum Resistance Gene PigmR to Rice Blast[J]. Chinese Journal OF Rice Science, 2025, 39(3): 365-372.

李文奇, 许扬, 王芳权, 朱建平, 陶亚军, 李霞, 范方军, 蒋彦婕, 陈智慧, 杨杰. 广谱抗稻瘟病基因PigmR的KASP标记开发及应用[J]. 中国水稻科学, 2025, 39(3): 365-372.

Figures/Tables 5

Fig. 1. Variation sites of partial PigmR coding region sequence in Gumei 4 The numbers represent the position in the PigmR coding region; Bold letters refer to the specific nucleotide variation site at 2503 bp in the coding region.

Table 1. PigmR-K marker information

引物名称 Primer	引物序列(5′→3′) Primer sequence(5′→3′)	引物长度和标记 Primer length and marker	结果 Result
Pigm-KR1	GAAGGTGACCAAGTTCATGCTCCTTTAGTTTACTCCTCCGTAAGTC	46 bp, Fam: Allele-C	C:C为感病基因型 C:C, Susceptible genotype
Pigm-KR2	GAAGGTCGGAGTCAACGGATTTCCTTTAGTTTGCTCCTCCGTAAGTA	47 bp, Hex: Allele-A	A:A为抗性基因型 A:A, Resistant genotype
Pigm-KF	TAACTGGATTGAGCAGCTCACTCACCTG	28 bp	A:C为杂合基因型 A:C, Heterozygous genotype

Fig. 2. PigmR-K marker verification A, Optimization of reaction conditions for the PigmR-K marker; B, Genotyping plot of PigmR-K in rice varieties. × represents blank control without DNA template. The red dots represent the Hex fluorescence signal, indicating homozygous resistant genotypes (A:A); The blue dots represent the Fam fluorescence signal, indicating homozygous susceptible genotypes (C:C); The green dots represent heterozygous genotypes (A:C).

Fig. 3. Genotyping of PigmR in rice F2 segregating population using PigmR-K marker A & B, Genotyping of PigmR in F2 progeny derived from a cross between Huaidao 5 (susceptible parent) and Jinxiangyu 1 (resistant parent) using the PigmR-K marker. C, Distribution of three PigmR genotypes detected by PigmR-K marker. D, Representative neck blast symptoms of selected F2 plants. × represents blank control without DNA template, the red dots represent the Hex fluorescence signal, indicating homozygous resistant genotypes (A:A), the blue dots represent the Fam fluorescence signal, indicating homozygous susceptible genotypes (C:C), and the green dots represent heterozygous genotypes (A:C).

Fig. 4. Genotyping of PigmR in backcross breeding population using PigmR-K marker A and B, Genotyping of PigmR in the BC1F3 population of Nanjing 53045/Jinxiangyu 1 using PigmR-K markers. × represents blank control without DNA template, the red dots represent the Hex fluorescence signal, indicating homozygous resistant genotypes (A/A), the blue dots represent the Fam fluorescence signal, indicating homozygous susceptible genotypes (C/C), and the green dots represent heterozygous genotypes (A/C).

References 35

[1]	Liu W D, Liu J L, Triplett L, Leach J E, Wang G L. Novel insights into rice innate immunity against bacterial and fungal pathogens[J]. Annual Review of Phytopathology, 2014, 52: 213-241.
[2]	Xiao N, Pan C H, Li Y H, Wu Y Y, Cai Y, Lu Y, Wang R Y, Yu L, Shi W, Kang H X, Zhu Z B, Huang N S, Zhang X X, Chen Z C, Liu J J, Yang Z F, Ning Y S, Li A H. Genomic insight into balancing high yield, good quality, and blast resistance of japonica rice[J]. Genome Biology, 2021, 22: 283.
[3]	Xiao N, Wu Y Y, Pan C H, Yu L, Chen Y, Liu G Q, Li Y H, Zhang X X, Wang Z P, Dai Z Y, Liang C Z, Li A H. Improving of rice blast resistances in japonica by pyramiding major R genes[J]. Frontiers in Plant Science, 2017, 7: 1918.
[4]	Li W T, Chern M S, Yin J J, Wang J, Chen X W. Recent advances in broad-spectrum resistance to the rice blast disease[J]. Current Opinion in Plant Biology, 2019, 50: 114-120.
[5]	Devanna B N, Jain P, Solanke A U, Das A, Thakur S, Singh P K, Kumari M, Dubey H, Jaswal R, Pawar D, Kapoor R, Singh J, Arora K, Saklani B K, AnilKumar C, Maganti S M, Sonah H, Deshmukh R, Rathour R, Sharma T R. Understanding the dynamics of blast resistance in rice-Magnaporthe oryzae interactions[J]. Journal of Fungi, 2022, 8: 584.
[6]	Wu Y, Xiao N, Yu L, Pan C H, Li Y H, Zhang X X, Liu G Q, Dai Z Y, Pan X B, Li A H. Combination patterns of major R genes determine the level of resistance to the M. oryzae in rice (Oryza sativa L.)[J]. PLoS ONE, 2015, 10(6): e0126130.
[7]	Wang B H, Ebbole D J, Wang Z H. The arms race between Magnaporthe oryzae and rice: Diversity and interaction of Avr and R genes[J]. Journal of Integrative Agriculture, 2017, 16: 2746-2760.
[8]	Deng Y W, Zhai k R, Xie Z, Yang D Y, Zhu X D, Liu J Z, Wang X, Qin P, Yang Y Z, Zhang G M, Li Q, Zhang J F, Wu S Q, Milazzo J, Mao B Z, Wang E T, Xie H A, Tharreau D, He Z H. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J]. Science, 2017, 355(6328): 962-965.
[9]	Wu Y Y, Xiao N, Chen Y, Yu L, Pan C H, Li Y H, Zhang X X, Huang N S, Ji H J, Dai Z Y, Chen X J, Li A H. Comprehensive evaluation of resistance effects of pyramiding lines with different broad-spectrum resistance genes against Magnaporthe oryzae in rice (Oryza sativa L.)[J]. Rice, 2019, 12: 11.
[10]	Zhai K R, Liang D, Li H L, Jiao F Y, Yan B X, Liu J, Lei Z Y, Huang L, Gong X Y, Wang X, Miao J S, Wang Y C, Liu J Y, Zhang L, Wang E T, Deng Y W, Wen C K, Guo H W, Han B, He Z H. NLRs guard metabolism to coordinate pattern- and effector-triggered immunity[J]. Nature, 2022, 601: 245-251.
[11]	吕学莲, 白海波, 惠建, 蔡正云, 张龙飞, 李树华. 利用广谱抗病基因Pigm改良水稻稻瘟病抗性[J]. 分子植物育种, 2017, 15(11): 4336-4342.
	Lü X L, Bai H B, Hui J, Cai Z Y, Zhang L F, Li S H. The improvement of rice blast resistance by broad-spectrum gene Pigm[J]. Molecular Plant Breeding, 2017, 15(11): 4336-4342. (in Chinese with English abstract)
[12]	Pariasca-tanaka J, Lorieux M, He C H, McCouch S S, Thomson M J, Wissuwa M. Development of a SNP genotyping panel for detecting polymorphisms in Oryza glaberrima/O. sativa interspecific crosses[J]. Euphytica, 2015, 201(1): 67-78.
[13]	金名捺, 陈竹锋, 丘式浚, 陈慧, 谢刚, 李早霞, 唐晓艳. 基于HRM体系的稻瘟病抗性基因 Pi2特异性分子标记的开发及应用[J]. 农业生物技术学报, 2018, 26(3): 365-373.
	Jin M N, Chen Z F, Qiu S J, Chen H, Xie G, Li Z X, Tang X Y. Development and application of HRM-based molecular marker specific for the Pi2 gene for rice blast resistance[J]. Journal of Agricultural Biotechnology, 2018, 26(3): 365-373. (in Chinese with English abstract)
[14]	Zhou Z, Sun L, Hu W B, Zhou B, Tao S H, Zhang S H, Lü Y M, Zhao Z H, Chen C Y. Breeding high-grain quality and blast resistant rice variety using combination of traditional breeding and marker-assisted selection[J]. Rice Science, 2021, 28(5): 422-432.
[15]	He C, Holme J, Anthony J. SNP genotyping: the KASP assay[J]. Methods in Molecular Biology, 2014, 1145: 75-86.
[16]	Shao Y, Peng Y, Mao B G, Lü Q M, Yuan D Y, Liu X L, Zhao B R. Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China[J]. PLoS One, 2020, 15(5): e0232279.
[17]	Addison C K, Angira B, Kongchum M, Harrell D L, Baisakh N, Linscombe S D, Famoso A N. Characterization of haplotype diversity in the BADH2 aroma gene and development of a KASP SNP assay for predicting aroma in US rice[J]. Rice, 2020, 13(1): 47.
[18]	张先文, 贺治洲, 江南, 邓华凤, 李继明. 高通量基因型分型技术及其在水稻中的应用[J]. 生物技术通报, 2017, 33(12): 67-73.
	Zhang X W, He Z Z, Jiang N, Deng H F, Li J M. High-throughput genotyping techniques and their applications in rice[J]. Biotechnology Bulletin, 2017, 33(12): 67-73. (in Chinese with English abstract)
[19]	陈涛, 孙旭超, 张善磊, 梁文化, 周丽慧, 赵庆勇, 姚姝, 赵凌, 赵春芳, 朱镇, 张亚东, 王才林. 稻瘟病广谱抗性基因Pigm特异性分子标记的开发和应用[J]. 中国水稻科学, 2020, 34(1): 28-36.
	Chen T, Sun X H, Zhang S L, Liang W H, Zhou L H, Zhao Q Y, Yao S, Zhao L, Zhao C F, Zhu Z, Zhang Y D, Wang C L. Development and verification of specific molecular markers for Pigm gene associated with broad-spectrum resistance to rice blast[J]. Chinese Journal of Rice Science, 2020, 34(1): 28-36.
[20]	Tang W J, Lin J, Wang Y P. An H Z, Chen H Y, Pan G, Zhang S B, Guo B W, Yu K, Li H Y, Fang X W, Zhang Y H. Selection and validation of 48 KASP markers for variety identification and breeding guidance in conventional and hybrid rice (Oryza sativa L.)[J]. Rice, 2022, 15: 48.
[21]	Yang G L, Chen S P, Chen L K. Development of a core SNP arrays based on the KASP method for molecular breeding of rice[J]. Rice, 2019, 12(21): 1-18.
[22]	李白, 蔡之军, 王蕾, 陈婕, 曹奎荣, 李军, 种高军. 抗稻瘟病基因Pigm组合标记的开发及应用[J]. 生物技术通报, 2022, 38(7): 153-159.
	Li B, Cai Z J, Wang L, Chen J, Cao K R, Li J, Zhong G J. Development and application of the combinatorial marker for the rice blast resistance gene Pigm[J]. Biotechnology Bulletin, 2022, 38(7): 153-159. (in Chinese with English abstract)
[23]	邓钊, 江南, 符辰建, 严天泽, 符星学, 胡小淳, 秦鹏, 刘珊珊, 王凯, 杨远柱. 隆两优与晶两优系列杂交稻的稻瘟病抗性基因分析[J]. 作物学报, 2022, 48(6): 1071-1080.
	Deng Z, Jiang N, Fu C J, Yan T Z, Fu X X, Hu X C, Qin P, Liu S S, Wang K, Yang Y Z. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties[J]. Acta Agronomica Sinica, 2022, 48(6): 1071-1080. (in Chinese with English abstract)
[24]	杨义强, 朱林峰, 李晓芳, 付杰, 黄道强, 邱先进, 周少川, 王重荣. 抗稻瘟病基因Pi2的基因特异性KASP标记开发与应用[J]. 植物遗传资源学报, 2021, 22(5): 1314-1321.
	Yang Y Q, Zhu L F, Li X F, Fu J, Huang D Q, Qiu X J, Zhou S C, Wang C R. Development and application of KASP marker specific for rice blast resistance Pi2 gene[J]. Journal of Plant Genetic Resources, 2021, 22(5): 1314-1321. (in Chinese with English abstract)
[25]	沈乐融, 齐中强, 杜艳, 于俊杰, 俞咪娜, 刘永锋. 江苏省稻瘟病菌致病力分化及无毒基因组成分析[J]. 江苏农业学报, 2019, 35(1): 6.
	Shen L R, Qi Z Q, Du Y, Yu J J, Yu M N, Liu Y F. Pathogenicity differentiation and composition analysis of avirulence genes of Magnaporthe oryzae in Jiangsu Province[J]. Jiangsu Journal of Agricultural Sciences, 2019, 35(1): 6. (in Chinese with English abstract)
[26]	齐中强, 刘永锋, 于俊杰, 俞咪娜, 杜艳, 曹慧娟, 宋天巧, 潘夏艳, 梁栋, 刘永锋. 江苏省2016-2020年水稻新品种(系)和主栽品种对稻瘟病的抗病性评价[J]. 江苏农业科学, 2022,50(1): 91-96.
	Qi Z Q, Liu Y F, Yu J J, Yu M N, Du Y, Cao H J, Song T Q, Pan X Y, Liang D, Liu Y F. Identification and evaluation of resistance of new rice cultivars (lines) and main cultivars to rice blast in Jiangsu Province from 2016 to 2020[J]. Jiangsu Agricultural Sciences, 2022, 50(1): 91-96. (in Chinese with English abstract)
[27]	中华人民共和国农业部.水稻品种试验稻瘟病抗性鉴定与评价技术规程: NY/T 2646-2014[S]. 北京: 中国标准出版社, 2014.
	Ministry of Agriculture and Rural Affairs of the People's Republic of China.Technical Specification for Identification and Evaluation of Blast Resistance in Rice Variety Region Test: NY/T 2646-2014[S]. Beijing: Standards Press of China, 2014. (in Chinese).
[28]	Feng Z M, Li M Y, Xu Z W, Gao P, Wu Y Y, Wu K T, Zhao J H, Wang X Q, Wang J N, Li M C, Hu K M, Chen H Q, Deng Y W, Li A H, Chen Z X, Zuo S M. Development of rice variety with durable and broad-spectrum resistance to blast disease through marker-assisted introduction of Pigm gene[J]. Frontiers in Plant Science, 2022, 13: 937767.
[29]	王芳权, 陈智慧, 许扬, 王军, 李文奇, 范方军, 陈丽琴, 陶亚军, 仲维功, 杨杰. 水稻广谱抗稻瘟病基因PigmR功能标记的开发及应用[J]. 中国农业科学, 2019, 52(6): 955-967.
	Wang F Q, Chen Z H, Xu Y, Wang J, Li W Q, Fan F J, Chen L Q, Tao Y J, Zhong W G, Yang J. Development and application of the functional marker for the broad-spectrum blast resistance gene PigmR in rice[J]. Scientia Agricultura Sinica, 2019, 52(6): 955-967. (in Chinese with English abstract)
[30]	杨好, 黄衍焱, 易春霖, 石军, 谭楮湉, 任文芮, 王文明. 水稻Pi9位点6个稻瘟病抗性基因特异分子标记开发及应用[J]. 中国农业科学, 2023, 56(21): 4219-4233.
	Yang H, Huang Y Y, Yi C L, Shi J, Tan C T, Ren W R, Wang W M. Development and application of specific molecular markers for six homologous rice blast resistance genes in Pi9 locus of rice[J]. Scientia Agricultura Sinica, 2023, 56(21): 4219-4233. (in Chinese with English abstract)
[31]	杨青青, 唐家琪, 张昌泉, 高继平, 刘巧泉. KASP标记技术在主要农作物中的应用及展望[J]. 生物技术通报, 2022, 38(4): 58-71.
	Yang Q Q, Tang J Q, Zhang C Q, Gao J P, Liu Q Q. Application and prospect of KASP marker technology in main crops[J]. Biotechnology Bulletin, 2022, 38(4): 58-71. (in Chinese with English abstract)
[32]	Nitika S, Jasneet S, Gursewak S, Sethi M, Singh M P, Pruthi G, Raigar O P, Kaur R, Kaur R, Sarao P S, Lore J S, Singh U M, Dixit S, Sagare D B, Singh S P, Satturu V, Singh V K, Kumar A. Development and validation of a novel core set of KASP markers for the traits improving grain yield and adaptability of rice under direct-seeded cultivation conditions[J]. Genomics, 2022, 14: 110269.
[33]	邓钊, 王凯, 杜波, 祝莉莉, 杨远柱, 陈荣智. 水稻抗褐飞虱基因Bph43紧密连锁KASP标记开发与验证[J]. 湖北农业科学, 2023, 62(6): 169-174.
	Deng Z, Wang K, Du B, Zhu L L, Yang Y Z, Chen R Z. Development and validation of a tightly linked KASP marker for the rice brown planthopper resistance gene Bph43[J]. Hubei Agricultural Sciences, 2023, 62(6): 169-174. (in Chinese with English abstract)
[34]	赵传超, 丛森, 梁思怡, 谢华斌, 陆文宇, 肖武名, 黄明, 郭涛, 王加峰. 水稻抗稻瘟病基因Pi2的KASP标记开发与应用[J]. 华南农业大学学报, 2023, 44(5): 725-734.
	Zhao C C, Cong S, Liang S Y, Xie H B, Lu W Y, Xiao W M, Huang M, Guo T, Wang J F. Development and application of KASP marker for rice blast resistance gene Pi2[J]. Journal of South China Agricultural University, 2023, 44(5): 725-734. (in Chinese with English abstract)
[35]	Yang G L, Chen S P, Chen L K, Sun K, Huang C H, Zhou D H, Huang Y T, Wang J F, Liu Y Z, Wang H, Chen Z Q, Guo T. Development of a core SNP arrays based on the KASP method for molecular breeding of rice[J]. Rice, 2019, 12: 21.

Development and Application of KASP Marker for Broad-Spectrum Resistance Gene PigmR to Rice Blast

广谱抗稻瘟病基因PigmR的KASP标记开发及应用

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