Chinese Journal OF Rice Science ›› 2025, Vol. 39 ›› Issue (4): 516-528.DOI: 10.16819/j.1001-7216.2025.250105
• Special Focus: Mechanization and Intelligentization in Rice Production • Previous Articles Next Articles
DONG Liqiang1,2, ZHANG Yikai2,*(), YANG Tiexin1, FENG Yingying1,3, MA Liang1, LIANG Xiao4, ZHANG Yuping2, LI Yuedong1,*(
)
Received:
2025-01-08
Revised:
2025-02-17
Online:
2025-07-10
Published:
2025-07-21
Contact:
ZHANG Yikai, LI Yuedong
董立强1,2, 张义凯2,*(), 杨铁鑫1, 冯莹莹1,3, 马亮1, 梁潇4, 张玉屏2, 李跃东1,*(
)
通讯作者:
张义凯,李跃东
基金资助:
DONG Liqiang, ZHANG Yikai, YANG Tiexin, FENG Yingying, MA Liang, LIANG Xiao, ZHANG Yuping, LI Yuedong. Effect of Dense Sowing Nursery on Seedling Quality and Picking Characteristics for Mechanized Transplanting in Northern japonica Rice[J]. Chinese Journal OF Rice Science, 2025, 39(4): 516-528.
董立强, 张义凯, 杨铁鑫, 冯莹莹, 马亮, 梁潇, 张玉屏, 李跃东. 北方粳稻密苗机插育秧对秧苗素质及取秧特性的影响[J]. 中国水稻科学, 2025, 39(4): 516-528.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2025.250105
基因 Gene | 正向引物 Forward sequence (5'-3') | 反向引物 Reverse sequence (5'-3') |
---|---|---|
Actin-OS-GAPDH | TACAGCTCTCGCGTTGTTGA | CATCATGCGAAAAGCCAGCA |
OsLPXC | TGAAAAATTGCGTTCTGCTG | GCAAGAAGCGAGAAATCACC |
OsSPX1 | AGCAGCAGGAACTGTGGAAT | CCCGGTACTATCTCCAGCAA |
Table 1. Primers used in the study
基因 Gene | 正向引物 Forward sequence (5'-3') | 反向引物 Reverse sequence (5'-3') |
---|---|---|
Actin-OS-GAPDH | TACAGCTCTCGCGTTGTTGA | CATCATGCGAAAAGCCAGCA |
OsLPXC | TGAAAAATTGCGTTCTGCTG | GCAAGAAGCGAGAAATCACC |
OsSPX1 | AGCAGCAGGAACTGTGGAAT | CCCGGTACTATCTCCAGCAA |
处理 Treatment | 横向取苗 Horizontal seedling width (mm) | 纵向取苗 Vertical seedling length(mm) | 取苗面积 Seedling area (mm2) | 理论取苗株数 Theoretical seedling number | 单盘理论取苗次数 Theoretical hill number per tray | 理论用苗盘数 Theoretical tray number(No./hm2) |
---|---|---|---|---|---|---|
CK | 14 | 14 | 196 | 4.43 | 828.57 | 251.45 |
ISR1 | 14 | 12 | 168 | 4.62 | 966.67 | 215.53 |
ISR2 | 14 | 10 | 140 | 4.43 | 1160.00 | 179.61 |
ISR3 | 11 | 11 | 121 | 4.46 | 1370.91 | 151.97 |
ISR4 | 11 | 10 | 110 | 4.74 | 1508.00 | 138.16 |
Table 2. Theoretical parameters of seedling picking characteristics for mechanical transplanting
处理 Treatment | 横向取苗 Horizontal seedling width (mm) | 纵向取苗 Vertical seedling length(mm) | 取苗面积 Seedling area (mm2) | 理论取苗株数 Theoretical seedling number | 单盘理论取苗次数 Theoretical hill number per tray | 理论用苗盘数 Theoretical tray number(No./hm2) |
---|---|---|---|---|---|---|
CK | 14 | 14 | 196 | 4.43 | 828.57 | 251.45 |
ISR1 | 14 | 12 | 168 | 4.62 | 966.67 | 215.53 |
ISR2 | 14 | 10 | 140 | 4.43 | 1160.00 | 179.61 |
ISR3 | 11 | 11 | 121 | 4.46 | 1370.91 | 151.97 |
ISR4 | 11 | 10 | 110 | 4.74 | 1508.00 | 138.16 |
Fig. 2. Biomass accumulation of rice seedlings under different sowing rates The same lowercase letters indicate that the difference between treatments is not significant at 0.05 level. The same as in the figures below.
处理 Treatment | 叶龄 Leaf age | SPAD | 株高 Plant height(cm) | 茎基宽 Stem base width(mm) |
---|---|---|---|---|
CK | 2.78±0.04 a | 29.26±0.56 a | 14.80±0.18 a | 2.17±0.03 a |
ISR1 | 2.54±0.03 b | 28.70±0.19 a | 14.48±0.19 ab | 1.79±0.02 b |
ISR2 | 2.47±0.03 bc | 28.44±0.69 a | 14.15±0.18 b | 1.72±0.02 c |
ISR3 | 2.44±0.03 c | 28.35±0.42 a | 13.19±0.17 c | 1.66±0.02 c |
ISR4 | 2.16±0.03 d | 28.05±0.38 a | 12.11±0.16 d | 1.58±0.02 d |
Table 3. Individual morphological characteristics of rice seedlings under different sowing rates
处理 Treatment | 叶龄 Leaf age | SPAD | 株高 Plant height(cm) | 茎基宽 Stem base width(mm) |
---|---|---|---|---|
CK | 2.78±0.04 a | 29.26±0.56 a | 14.80±0.18 a | 2.17±0.03 a |
ISR1 | 2.54±0.03 b | 28.70±0.19 a | 14.48±0.19 ab | 1.79±0.02 b |
ISR2 | 2.47±0.03 bc | 28.44±0.69 a | 14.15±0.18 b | 1.72±0.02 c |
ISR3 | 2.44±0.03 c | 28.35±0.42 a | 13.19±0.17 c | 1.66±0.02 c |
ISR4 | 2.16±0.03 d | 28.05±0.38 a | 12.11±0.16 d | 1.58±0.02 d |
Fig. 5. Uniformity of plant height and stem base width under different sowing rates Uni-PIH, Uniformity of plant height(%); Uni-SbW, Uniformity of stem base width(%).
处理 Treatment | 总根长 Total root length(cm) | 根总表面积 Total root surface area(cm2) | 根总体积 Total volume of roots(mm3) | 平均直径 Average diameter(mm) |
---|---|---|---|---|
CK | 79.33±1.80 a | 7.80±0.18 a | 57.99±1.31 a | 0.87±0.02 a |
ISR1 | 76.71±1.04 ab | 7.56±0.10 ab | 57.13±0.78 a | 0.84±0.01 ab |
ISR2 | 76.55±1.58 ab | 7.44±0.15 bc | 55.44±1.15 a | 0.83±0.02 ab |
ISR3 | 75.04±0.85 b | 7.19±0.08 c | 52.01±0.59 b | 0.83±0.01 b |
ISR4 | 70.82±1.21 c | 6.62±0.11 d | 48.63±0.83 c | 0.82±0.01 b |
Table 4. Root traits of rice seedlings under different sowing rates
处理 Treatment | 总根长 Total root length(cm) | 根总表面积 Total root surface area(cm2) | 根总体积 Total volume of roots(mm3) | 平均直径 Average diameter(mm) |
---|---|---|---|---|
CK | 79.33±1.80 a | 7.80±0.18 a | 57.99±1.31 a | 0.87±0.02 a |
ISR1 | 76.71±1.04 ab | 7.56±0.10 ab | 57.13±0.78 a | 0.84±0.01 ab |
ISR2 | 76.55±1.58 ab | 7.44±0.15 bc | 55.44±1.15 a | 0.83±0.02 ab |
ISR3 | 75.04±0.85 b | 7.19±0.08 c | 52.01±0.59 b | 0.83±0.01 b |
ISR4 | 70.82±1.21 c | 6.62±0.11 d | 48.63±0.83 c | 0.82±0.01 b |
处理 Treatment | SOD活性 SOD activity (U/g) | POD活性 POD activity (U/g) | CAT活性 CAT activity (U·g/min) | MDA活性 MDA activity (nmol/g) | 可溶性糖 Soluble protein content (U·g/g) | 可溶性蛋白 Soluble sugar content (mg/g) |
---|---|---|---|---|---|---|
CK | 350.36±7.94 a | 12.10±0.27 a | 45.25±1.03 a | 0.45±0.01 d | 1.36±0.03 a | 17.61±0.40 a |
ISR1 | 343.29±4.66 a | 11.79±0.16 a | 44.46±0.60 a | 0.46±0.01 d | 1.32±0.02 ab | 17.59±0.24 a |
ISR2 | 340.37±7.03 a | 10.79±0.22 b | 44.14±0.91 ab | 0.49±0.01 c | 1.28±0.03 bc | 16.99±0.35 ab |
ISR3 | 320.18±3.61 b | 9.57±0.11 c | 42.35±0.48 bc | 0.52±0.01 b | 1.25±0.01 c | 16.60±0.19 b |
ISR4 | 302.31±5.18 c | 8.99±0.15 d | 41.25±0.71 c | 0.56±0.01 a | 1.24±0.02 c | 15.46±0.26 c |
Table 5. Antioxidant enzyme activities in rice seedling leaves under different sowing rates
处理 Treatment | SOD活性 SOD activity (U/g) | POD活性 POD activity (U/g) | CAT活性 CAT activity (U·g/min) | MDA活性 MDA activity (nmol/g) | 可溶性糖 Soluble protein content (U·g/g) | 可溶性蛋白 Soluble sugar content (mg/g) |
---|---|---|---|---|---|---|
CK | 350.36±7.94 a | 12.10±0.27 a | 45.25±1.03 a | 0.45±0.01 d | 1.36±0.03 a | 17.61±0.40 a |
ISR1 | 343.29±4.66 a | 11.79±0.16 a | 44.46±0.60 a | 0.46±0.01 d | 1.32±0.02 ab | 17.59±0.24 a |
ISR2 | 340.37±7.03 a | 10.79±0.22 b | 44.14±0.91 ab | 0.49±0.01 c | 1.28±0.03 bc | 16.99±0.35 ab |
ISR3 | 320.18±3.61 b | 9.57±0.11 c | 42.35±0.48 bc | 0.52±0.01 b | 1.25±0.01 c | 16.60±0.19 b |
ISR4 | 302.31±5.18 c | 8.99±0.15 d | 41.25±0.71 c | 0.56±0.01 a | 1.24±0.02 c | 15.46±0.26 c |
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