Effect of Temperature on Development and Parasitizing Capacity of Trichogramma japonicum Reared on the Eggs of Rice Moth (Corcyra cephalonica)

doi:10.16819/j.1001-7216.2018.7134

Abstract

Abstract:

【Objective】The research aims to reveal the effects of gradient constant temperature on development and parasiting ability of Trichogramma japonicum reared on the eggs of rice moth(Corcyra cephalonica) .【Methods】The life table of the experimental population of T. japonicum from Jilin Province at five different temperatures (15℃, 20℃, 25℃, 30℃ and 33℃) was constructed. The developmental threshold temperature and effective accumulative temperature for whole generation were calculated based on the developmental duration and rate. Then, the linear regression model was applied to simulate the growth rate. 【Results】With increasing temperature, the number of host eggs parasitized per females rose first and then fell. The largest number was 29.75 at 30℃, and there was no significant difference between 25℃ and 30℃. However, it was obviously higher than that of the other three temperatures. The females exhibited a significant decrease in longevity as the temperature increased. The maximum survival time of females was at 15℃, longer than other temperatures. When the temperature was over 30℃, the females survived for only 0.82 days. The highest emergence rate, came at 25℃, was 98.14%, while the lowest emergence rate was 67.96%. The developmental rate was remarkably linear correlative with temperature. The developmental threshold temperature and effective accumulative temperature of whole generation were 11.49℃ and 131.39℃ per day, respectively. 【Conclusion】The temperature was the most important factor for the population growth of T. japonicum. It was concluded that the best suitable temperature for industrialized reproduction ranged from 25℃ to 30℃.

Key words: Trichogramma japonicum, temperature, life table, developmental temperature, effective accumulative temperature.

摘要：

【目的】本研究旨在明确不同温度下稻螟赤眼蜂在米蛾卵上的生长发育状况及寄生潜能。【方法】分别在15℃、20℃、25℃、30℃、33℃下,编制了吉林稻螟赤眼蜂在米蛾卵上的生命表,根据不同温度下稻螟赤眼蜂的发育历期、发育速率计算世代发育起点温度、世代有效积温,并采用线性回归模型对发育速率进行拟合。【结果】稻螟赤眼蜂平均单雌寄生米蛾卵数随着温度的升高呈现先升高后降低的趋势,30℃时最高,达29.75粒,除与25℃下无显著差异外,均显著高于其他温度;雌蜂平均寿命随温度升高而下降,15℃时寿命显著长于其他温度,当温度高于30℃时,其寿命均短于0.82d;25℃时,稻螟赤眼蜂羽化率最高,达98.14%;15℃时,羽化率最低,只有67.96%。稻螟赤眼蜂的发育速率与温度呈显著线性关系,其发育起点温度为11.49℃,世代有效积温为131.39℃·d。【结论】利用米蛾卵繁殖稻螟赤眼蜂时,温度对其有显著影响,综合生命表参数、单雌寄生米蛾卵数、雌蜂寿命、羽化率等指标,提高利用米蛾卵工厂化繁殖稻螟赤眼蜂的最佳温度为25℃~30℃。

关键词: 稻螟赤眼蜂, 温度, 生命表, 发育起点温度, 有效积温

CLC Number:

Changchun RUAN, Ruotian GUO, Xiaoxuan HU, Wenmei DU, Liansheng ZANG, Junjie ZHANG. Effect of Temperature on Development and Parasitizing Capacity of Trichogramma japonicum Reared on the Eggs of Rice Moth (Corcyra cephalonica)[J]. Chinese Journal OF Rice Science, 2018, 32(4): 398-404.

阮长春, 郭若天, 胡晓暄, 杜文梅, 臧连生, 张俊杰. 温度对稻螟赤眼蜂在米蛾卵上的生长发育和寄生潜能的影响[J]. 中国水稻科学, 2018, 32(4): 398-404.

Figures/Tables 3

References 40

[1]	张帅, 李建洪, 张绍明, 张凯雄, 周群芳. 杀虫剂对长江中下游地区水稻害虫田间防效. 农药, 2016, 55(7): 530-535.
	Zhang S, Li J H, Zhang S M, Zhang K X, Zhou Q F.Control efficacy of insecticides on the rice insect pests in the Middle and Lower Reaches of Yangtze River.Agrochemicals, 2016, 55(7): 530-535. (in Chinese with English abstract)
[2]	何月平, 邵振润, 陈文明, 梁桂梅, 李永平, 周威君, 沈晋良. 防治水稻二化螟的高毒农药替代药剂的室内筛选. 中国水稻科学, 2008, 22(3): 313-320.
	He Y P, Shao Z R, Chen W M, Liang G M, Li Y P, Zhou W J, Shen J L.Laboratory screening of alternative insecticides for highly toxic insecticides against the striped stem borer(Chilosuppressalis) on rice. Chin J Rice Sci, 2008, 22(3): 313-320. (in Chinese with English abstract)
[3]	李凤良, 金剑雪, 李忠英, 胡茂森, 徐秋芳, 陈之浩. 21种杀虫剂对稻纵卷叶螟幼虫触杀毒力比较. 植物保护, 2005, 31(2): 85-87.
	Li F L, Jin J X, Li Z Y, Hu M S, Xu Q F, Chen Z H.Comparison of contact toxicity of 21 insecticides against larvae of rice leaf roller.Plant Protect, 2005, 31(2): 85-87. (in Chinese with English abstract)
[4]	赵丹丹, 周丽琪, 张帅, 姚蓉, 邱运霞, 高聪芬. 二化螟对双酰胺类杀虫剂的抗药性监测和交互抗性研究. 中国水稻科学, 2017, 31(3): 307-314.
	Zhao D D, Zhou L Q, Zhang S, Yao R, Qiu Y X, Gao C F.Resistance monitoring and cross-resistance to the diamides in the rice stem borer,Chilosuppressalis(Lepidoptera: Pyralidae). Chin J Rice Sci, 2017, 31(3): 307-314. (in Chinese with English abstract)
[5]	池仕运, 彭宇, 王荫长, 韩召军, 陈长琨. 二化螟对杀虫剂抗药性的研究进展. 植物保护, 2005, 31(6): 3-6.
	Chi S Y, Peng Y, Wang Y C, Han Z J, Chen C K.Advances in the research of insecticide resistance of Chilo Suppressalis. Plant Protect, 2005, 31(6): 3-6. (in Chinese with English abstract)
[6]	任秀贝. 稻纵卷叶螟对杀虫剂抗性的监测与室内饲养技术研究. 南京: 南京农业大学, 2013
	Ren X B.Monitoring of insecticide resistance and the research on artificial diets for rice leaffolder, Cnaplocrocis Medinalis(Guenee )(Lepidoptera: Pyralidae). Nanjing: Nanjing Agricultural University, 2013. (in Chinese with English abstract)
[7]	董本春, 李晓光, 高德宇, 张富满. 螟黄赤眼蜂防治水稻二化螟的研究. 植物保护, 2001, 27(4):45-46
	Dong B C, Li X G, Gao D Y, Zhang F M.Study of Trichogramma Chilonis rice stem borer. Plant Protect, 2001, 27(4): 45-46. (in Chinese with English abstract)
[8]	杭德龙, 焦兆文, 赵有文, 杨学文, 夏必文, 孙蔚. 稻田释放拟澳洲赤眼蜂防治一代二化螟研究. 农业灾害研究, 2011, 1(1): 28-30.
	Hang D L, Jiao Z W, Zhao Y W, Yang X W, Xia B W, Sun W.Control of first generation Chilosuppressalis by releasing Trichogramma ConfusumViggiani in rice field. J Agric Catastrophol, 2011, 1(1): 28-30. (in Chinese with English abstract)
[9]	康敏, 赵剑锋, 顾中量. 德宏州芒市运用赤眼蜂防治水稻螟虫效果初探. 云南农业科技, 2015,(5): 54-55.
	Kang M, Zhao J F, Gu Z L.Mans use ofTrichogramma to control rice stem borer effect in Dehong. Yunnan Agric Sci and Technol, 2015(5): 54-55. (in Chinese with English abstract)
[10]	Zhang J J, Ren B Z, Yuan X H, Zang L S, Ruan C C, Sun G Z, Shao X W.Effects of host-egg ages on host selection and suitability of four chinese Trichogramma species, egg parasitoids of the rice striped stem borer, Chilosuppressalis. Bio Control, 2014, 59(2): 159-166.
[11]	Yuan X H, Song L W, Zhang J J, Zang L S, Zhu L, Ruan C C, Sun G Z.Performance of four Chinese Trichogramma species as biocontrol agents of the rice striped stem borer,Chilosuppressalis, under various temperature and humidity regimes. J Pest Sci, 2012, 85(4): 497-504.
[12]	郭震, 阮长春, 臧连生, 张帆, 靳锋云. 稻螟赤眼蜂rDNA特异引物设计及诊断引物在赤眼蜂分子鉴定中的应用. 中国水稻科学, 2012, 26(1): 123-126.
	Guo Z, Ruan C C, Zang L S, Zhang F, Jin F Y.Design of specific primer for Trichogramma japonicum based on rDNA-ITS2 and application of diagnostic primers in identification of four Trichogramma species. Chin J Rice Sci, 2012, 26(1): 123-126. (in Chinese with English abstract)
[13]	郭震, 阮长春, 臧连生, 张帆, 靳锋云. 吉林省稻螟赤眼蜂的发现和鉴定. 中国生物防治学报, 2011, 27(2): 276-279.
	Guo Z, Ruan C C, Zang L S, Zhang F, Jin F Y.The new record of Trichogramma japonicum Ashmead, an Egg parasitoid of Chilosuppressalis(Walker) in Jilin, China. Chin J Biol Con, 2011, 27(2): 276-279. (in Chinese with English abstract)
[14]	黄志农, 张玉烛, 朱国奇, 文吉辉, 李卫东, 方宝华, 刘洋, 夏青华, 刘功朋. 稻螟赤眼蜂防控稻纵卷叶螟和二化螟的效果评价. 江西农业学报, 2012, 24(5): 37-40.
	Huang Z N, Zhang Y Z, Zhu G Q, Wen J H, Li W D, Fang B H, Liu Y, Xia Q H, Liu G P.Evaluation of control effect of Trichogramma japonicum against Cnaphalocrocis medinalis and Chilosuppressalis. Acta Agric Jiangxi, 2012, 24(5): 37-40. (in Chinese with English abstract)
[15]	张俊杰, 阮长春, 臧连生, 邵玺文, 史树森. 我国赤眼蜂工厂化繁育技术改进及防治农业害虫应用现状. 中国生物防治学报, 2015, 31(5): 638-646.
	Zhang J J, Ruan C C, Zang L S, Shao X W, Shi S S.Technological improvements for mass production of Trichogramma and current status of their applications for biological control on agricultural pests in China.Chin J Biol Control, 2015, 31(5): 638-646. (in Chinese with English abstract)
[16]	陈洪凡, 黄寿山, 张玉烛, 曾翔, 黄泽辉. 稻螟赤眼蜂对二化螟和台湾稻螟的控制潜能评价. 应用生态学报, 2010, 21(3): 743-748.
	Chen H F, Huang S S, Zhang Y Z, Zeng X, Huang Z H.Control efficacy of Trichogramma japonicum against Chilosuppressalis and Chilotraea auricilia. Chin J Appl Ecol, 2010, 21(3): 743-748. (in Chinese with English abstract)
[17]	李莹, 董辉, 丛斌, 钱海涛, 杨海霞, 张柱亭, 孙巍. 温度对松毛虫赤眼蜂不同地理种群寄生潜能及种群参数的影响. 中国生物防治学报, 2013, 29(1): 11-16.
	Li Y, Dong H, Cong B, Qian H T, Yang H X, Zhang Z T, Sun W.Effect of temperature on reproductive potential and population parameters of different Trichogramma dendrolimi geographic populations. Chin J Biol Control. 2013, 29(1):11-16. (in Chinese with English abstract)
[18]	Hassan S A.Strategies to Select Trichogramma Species for use in Biological Control//Wajnberg E, Hassan S A. Biological Control with Other Egg Parasitoids. Wallingford, UK: CAB International Press, 1994: 55-71.
[19]	Flander S E.Mass Production of Egg Parasites of Genus Trichogramma. Hilgardia, 1930, 1: 465-501.
[20]	Flander S E. Storage Production.Econ Entomol, 1934, 27: 1197.
[21]	Lewis W J, Nordlund D A, Gross H R, Perkins W D, Knipling E F.Production and performance of Trichogramma reared on eggs of Helio-this zea and other hosts. Environ Entomol, 1976, 5(3): 449-452.
[22]	李丽英. 赤眼蜂研究应用新进展. 应用昆虫学报, 1984(5): 48-52.
	Li L Y.New Progress in research and application ofTrichogramma. J Appl Entom, 1984(5): 48-52. (in Chinese with English abstract)
[23]	Keinmeesuke P,Vattanatangum A, Sarnthoy O, Sayampol B, Miyata T, Salto T, Nakasujl F, Sinchaisri N.Life table of diamond backmoth and its egg parasite Trichogrammatoidea Bactrae in Thailand. Diamondback Moth & Other Crucifer Pests: Second International Workshop, 1992: 309-315.
[24]	Talekar N S.Biological control of diamondback moth in Taiwan: A Review.Plant Prot Bull, 1996, 21(38): 167-189.
[25]	Naranjo S E.Life history of Trichogrammatoidea bactrae, an egg parasitoid of pink bodworm, with emphasison performance at high temperatures. Environ Entomol, 1993, 22(5): 1051-1059.
[26]	宋静, 黄静, 王雷英, 李元喜. 繁育寄主对稻螟赤眼蜂寄生行为及寄生能力的影响. 昆虫学报, 2015, 58(7), 783-790.
	Song J, Huang J, Wang L Y, Li Y X.Effects of rearing host on parasitization behavior and capacity of Trichog ramma japonicum(Hymenoptera: Trichogrammatidae). Acta Entomol Sin, 2015, 58(7), 783-790. (in Chinese with English abstract)
[27]	阮长春, 史树森, 孙光芝, 张俊杰, 臧连生, 杜文梅. 一种米蛾成虫收集的新方法: ZL201210138981.7.2016-02-03.
	Ruan C C, Shi S S, Sun G Z, Zhang J J, Zang L S, Du W M. A New Method for Collecting Adult Worm of Rice Moth: ZL201210138981.7.2016-02-03. (in Chinese)
[28]	臧连生, 阮长春, 邵玺文, 孙光芝, 张俊杰, 李天昊, 刘志, 王秀梅, 杜文梅. 适合水田释放寄生蜂防治害虫的方法及释放装置: ZL201310360029.6.2015-10-21.
	Zang L S, Ruan C C, Shao X W, Sun G Z, Zhang J J, Li T H, Liu Z, Wang X M, Du W M. Method and Releasing Device for Releasing Parasitic Wasps to Control Insect Pests in Paddy Field: ZL201310360029.6.2015-10-21. (in Chinese)
[29]	杜文梅, 阮长春, 张俊杰, 史树森, 臧连生, 孙光芝, 李天昊, 王秀梅, 刘志, 刘显娇. 一种适合规模化繁育的米蛾饲养装置: ZL201520417931.1.2015-10-28.
	Du W M, Ruan C C, Zhang J J, Shi S S, Zang L S, Sun G Z, Li T H, Wang X M, Liu Z, Liu X J. A Suitable for Large-scale Breeding of Rice Moth Feeding Device: ZL201520417931.1.2015-10-28. (in Chinese)
[30]	张俊杰, 杜文梅, 阮长春, 臧连生, 彭海娇, 孙光芝. 不同饲料配方对米蛾生长发育及繁殖的影响. 吉林农业大学学报, 2012, 34(6): 603-606, 611.
	Zhang J J, Du W M, Ruan C C, Zang L S, Peng H J, Sun G Z.Effect of different artificial diets on growth, development and fecundity of Corcyra cephalonice. J Jilin Agric Univ, 2012, 34(6): 603-606, 611. (in Chinese with English abstract)
[31]	黄寿山, 戴志一, 吴达璋.赤眼蜂实验种群生命表的编制与应用. 植物保护学报, 1996, 23(3): 209-212.
	Huang S S, Dai Z Y, Wu D, Z. The establishment and application of the experimental population life tables of Trichogramma spp. on dieferent hosts. Acta Phytophyl Sin, 1996, 23(3): 209-212. (in Chinese with English abstract)
[32]	何余容, 吕利华, 庞雄飞. 小菜蛾自然种群连续世代生命表的组建与分析. 华南农业大学学报, 2000, 21(1): 34-37.
	He Y R, Lv L H, Pang X F.Parasitizing Ability and interspecific competition of Trichogramma confusum Viggiani and T. pretiosum riley on the eggs of Plutellaxylostella (L.) in the Laboratory. J South China Agric Univ, 2000, 21(1): 34-37. (in Chinese with English abstract)
[33]	徐汝梅. 昆虫种群生态学. 北京: 北京师范大学出版社, 1987: 61-82.
	Xu R M. Insect Population Ecology. Beijing: Beijing Normal University press, 1987: 61-82. (in Chinese with English abstract)
[34]	张孝羲. 昆虫生态及预测预报. 北京: 中国农业出版社, 2004: 218-219.
	Zhang X X.Insect Ecology and Prediction. Beijing: China Agriculture Press, 2004: 218-219. (in Chinese with English abstract)
[35]	陈鹏, 王凤珍, 李春成, 王秀梅, 阮长春. 榆紫叶甲赤眼蜂寄生功能反应对梯度恒温的响应. 东北林业大学学报, 2015, 43(1): 114-116.
	Chen P, Wang F Z, Li C C, Wang X M, Ruan C C.Effect of temperature on functional response of Asynactaam brostomae. J Northeast For Univ, 2015, 43(1): 114-116. (in Chinese with English abstract)
[36]	陈洪凡, 岑冠军, 黄寿山. 赤眼蜂发育速率对梯度恒温的响应. 生态学报, 2010, 30(23): 6669-6673.
	Chen H F, Cen G J, Huang S S.Effect of temperature on the development of Trichogramma species. Acta Ecol Sin, 2010, 30(23): 6669-6673.
[37]	Boivin G.Phenotypic Plasticity and fitness in egg parasitoids.Neotrop Entomol, 2010, 39: 457-463. (in Chinese with English abstract)
[38]	王建军, 魏建荣, 王玉珠, 张永超. 舞毒蛾卵寄生蜂大蛾卵跳小蜂发育与温度的关系及利用替代寄主柞蚕卵繁育的子代品质评价. 昆虫学报, 2012, 55(5): 570-574.
	Wang J J, Wei J R, Wang Y Z, Zhang Y C.Relationship between temperature and development of Ooencyrtus Kuwanae(Hymenopters:Encyrtidae) the egg parasitoid of Lymantriidae, with an evaluation of its offspring quality reared from eggs of the substitute host Antheraeapernyi (Lepidoptera: Saturniidae).. Acta Entomol Sin,, 2012, 55(5): 570-574. (in Chinese with English abstract)
[39]	张世泽, 郭建英, 万方浩, 张帆. 温度对不同品系丽蚜小蜂发育、存活和寿命的影响. 中国生物防治学报, 2004, 20(3): 174-177.
	Zhang S Z, Guo J Y, Wan F H, Zhang F.Effect of temperature on the development survival and longevity of Encarsia formosa. Chin J Biol Control, 2004, 20(3): 174-177. (in Chinese with English abstract)
[40]	李丽英, 张月华, 张荣华. 赤眼蜂生长发育与温度关系的种间及种内差异. 昆虫天敌, 1983, 5(1): 1-5.
	Li L Y, Zhang Y H, Zhang R H.The relationship between the growth and temperature of Trichogramma species and intraspecific differences. Nat Enem Insects, 1983, 5(1): 1-5. (in Chinese)

温度 Temperature/℃	日期 Date	发育历期 Developmental duration/d	存活雌蜂数 No. of surviving females	l_x	逐日寄生卵数 Daily parasitic eggs	逐日产雌数 Daily female progeny laid	m_x	l_xm_x	xl_xm_x
15	05–26	0
	06–24	29	74	1	752	462	6.2432	6.2432	181.0541
	06–25	30	52	0.703	53	8	0.1538	0.1081	3.2432
	06–26	31	4	0.054	3	0	0	0	0
	06–27	32	0	0	0	0	0	0	0
	合计Total				808	470	6.3971	6.3514	184.2973
20	07–31	0
	08–19	19	66	1	1404	1008	15.2727	15.2727	290.1818
	08–20	20	2	0.03	101	36	18	0.5455	10.9091
	08–21	21	0	0	0	0	0	0	0
	合计Total				1505	1044	33.2727	15.8182	301.0909
25	05–06	0
	05–15	9	54	1	1576	1364	25.2593	25.2593	227.3333
	05–15	10	0	0	0	0	0	0	0
	合计Total				1576	1364	25.2593	25.2593	227.3333
30	05–09	0
	05–16	7	57	1	1693	1439	25.2456	25.2456	176.7193
	05–17	8	0	0	0	0	0	0	0
	合计Total				1693	1439	25.2456	25.2456	176.7193
33	05–16	0
	05–22	6	67	1	426	259	3.8657	3.8657	23.19403
	05–23	7	0	0	0	0	0	0	0
	合计Total				426	259	3.8657	3.8657	23.19403

温度 Temperature/℃	日期 Date	发育历期 Developmental duration/d	存活雌蜂数 No. of surviving females	l_x	逐日寄生卵数 Daily parasitic eggs	逐日产雌数 Daily female progeny laid	m_x	l_xm_x	xl_xm_x
15	05–26	0
	06–24	29	74	1	752	462	6.2432	6.2432	181.0541
	06–25	30	52	0.703	53	8	0.1538	0.1081	3.2432
	06–26	31	4	0.054	3	0	0	0	0
	06–27	32	0	0	0	0	0	0	0
	合计Total				808	470	6.3971	6.3514	184.2973
20	07–31	0
	08–19	19	66	1	1404	1008	15.2727	15.2727	290.1818
	08–20	20	2	0.03	101	36	18	0.5455	10.9091
	08–21	21	0	0	0	0	0	0	0
	合计Total				1505	1044	33.2727	15.8182	301.0909
25	05–06	0
	05–15	9	54	1	1576	1364	25.2593	25.2593	227.3333
	05–15	10	0	0	0	0	0	0	0
	合计Total				1576	1364	25.2593	25.2593	227.3333
30	05–09	0
	05–16	7	57	1	1693	1439	25.2456	25.2456	176.7193
	05–17	8	0	0	0	0	0	0	0
	合计Total				1693	1439	25.2456	25.2456	176.7193
33	05–16	0
	05–22	6	67	1	426	259	3.8657	3.8657	23.19403
	05–23	7	0	0	0	0	0	0	0
	合计Total				426	259	3.8657	3.8657	23.19403

温度 Temperature/℃	净生殖力R₀ Net fecundity	世代平均周期T Mean generation time	瞬时增长率r Intrinsic rate of natural increase	周限增长率λ Finite rate of increase	单雌寄生卵数 Number of eggs parasitized	雌蜂寿命 Longevity of female/d	羽化率 Percentage of emergence/%
15	6.3514	29.0170	0.0637	1.0658	10.43±0.08 c	2.24±0.02 a	67.96±2.65 c
20	15.8182	19.0345	0.1451	1.1561	22.49±0.32 b	1.62±0.01 b	78.47±0.57 b
25	25.2593	9.0000	0.3588	1.4316	29.19±1.17 a	1.11±0.06 c	98.14±0.37 a
30	25.2456	7.0000	0.4612	1.5860	29.75±0.86 a	0.82±0.01 d	96.62±3.78 a
33	3.8657	6.0000	0.2254	1.2528	6.37±0.28 d	0.69±0.01 e	82.12±2.07 b

温度 Temperature/℃	净生殖力R₀ Net fecundity	世代平均周期T Mean generation time	瞬时增长率r Intrinsic rate of natural increase	周限增长率λ Finite rate of increase	单雌寄生卵数 Number of eggs parasitized	雌蜂寿命 Longevity of female/d	羽化率 Percentage of emergence/%
15	6.3514	29.0170	0.0637	1.0658	10.43±0.08 c	2.24±0.02 a	67.96±2.65 c
20	15.8182	19.0345	0.1451	1.1561	22.49±0.32 b	1.62±0.01 b	78.47±0.57 b
25	25.2593	9.0000	0.3588	1.4316	29.19±1.17 a	1.11±0.06 c	98.14±0.37 a
30	25.2456	7.0000	0.4612	1.5860	29.75±0.86 a	0.82±0.01 d	96.62±3.78 a
33	3.8657	6.0000	0.2254	1.2528	6.37±0.28 d	0.69±0.01 e	82.12±2.07 b