Chinese Journal of Rice Science

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Histochemical and Ultrastructural Demonstration of Peroxidase Activity During Infection of Rice by Magnaporthe grisea

YANG Min-he 1; 2; ZHENG Zhong 2; Jan E. LEACH 3   

  1. (1 Department of Plant Protection; College of Agriculture and Biotechnology; Zhejiang University; Hangzhou 310029; China; 2College of Agronomy; Jiangxi Agricultural University; Nanchang 330045, China; 3 Kansas State University, Manhattan, Kansas 66506, USA)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-01-10 Published:2002-01-10

水稻受稻瘟病菌侵染后过氧化物酶定位的超微观察

杨民和 1,2; 郑 重 1; Jan E. LEACH 3   

  1. 1浙江大学 农业与生物技术学院 植物保护系, 浙江 杭州 310029; 2 江西农业大学 农学院, 江西 南昌 330045; 3 堪萨斯州立大学, 美国 堪萨斯 66506

Abstract: Active oxygen species (AOS) and peroxidase are believed to involve in many important processes in plants in general and in plant-pathogen interaction in particular. Using DAB (3,3’-diaminobenzidine) staining method, the subcellular localization of H2O2 and peroxidase in the inner epidermal cells of rice leaf sheaths infected with compatible and incompatible races of Magnaporthe grisea were detected successfully. DAB staining was detected at the wounding, guard cells and penetration sites of M. grisea. Compared with compatible interaction, H2O2 and peroxidase accumulated quickly at the fungal penetration sites in incompatible interaction during initial stage of infection. An increase in the level of H2O2 and peroxidase activities in the incompatible interaction was also observed. Histochemical analysis of peroxidase distribution by electron microscopy revealed a striking, highly localized increase in peroxidase activities in the cell wall, plasma membrane, vesicle membrane and around invading hyphae of M. grisea in the penetrated host cells. However, the accumulation of peroxidase activities was delayed and at a much lower level in the penetrated host cells in compatible interaction.

Key words: rice blast, hydrogen peroxide, peroxidase, histochemistry, ultrastructure

摘要: 利用联苯胺蓝(3,3'-diaminobenzidine, DAB)染色法原位检测了水稻稻瘟病菌互作过程中H2O2和过氧化物酶被诱导产生和积累的过程。结果表明在病原菌接种后,水稻叶鞘内表皮细胞在伤口、气孔保卫细胞及病菌侵染点等3种情况下可以检测到染色反应。在水稻-稻瘟病菌非亲和性互作中,H2O2产生和过氧化物酶活性上升快,并逐渐积累到较高的水平;而在亲和性互作反应中,H2O2产生和过氧化物酶活性上升被延迟,积累水平较低。超微结构研究表明,在非亲和性互作反应中,过氧化物酶主要定位于被侵染寄主细胞的细胞壁、细胞质、细胞膜、侵染菌丝周围及由膜系统构成的囊泡膜上;而在亲和性互作反应中,早期(接种后16 h)几乎难以观察到过氧化物酶的聚集,后期(接种后30 h)过氧化物酶聚集增多,但仍明显低于非亲和性互作反应。

关键词: 稻瘟病, 过氧化氢, 过氧化物酶, 细胞化学, 超微结构