Identification and Gene Mapping of a Spotted-leaf Mutant hm197 in Rice

doi:10.3969/j.issn.1001G7216.2015.05.001

Abstract

Abstract:

A stable inherited rice spotted-leaf mutant hm197 was isolated from a diepoxybutane-induced IR64 mutant bank. Under natural conditions, brown lesions were first observed on the tips of the mutant leaves 10 weeks after sowing, and spread gradually downward to cover the whole leaf blades. Genetic analysis indicated that the mutant trait was controlled by a novel single recessive nuclear gene, tentatively termed as spl^hm97,located in the long arm of chromosome 4. Agronomic traits including the plant height, seed-setting rate and 1000-grain weight were significantly decreased in hm197. The initiation of brown lesions in hm197 was induced by natural sun light. In addition, the photosynthetic pigment contents and net photosynthetic rate in the mutant were significantly lower than those in the wild type IR64. Histochemical analysis showed that H₂O₂ and accumulation were presented in and around the lesions in hm197. Furthermore, senescence-related parameters including the activities of SOD, APX,total soluble protein and MDA contents were also significantly altered in the mutant while resistance to bacterial blight pathogens was largely enhanced in the mutant in contrast to the wild type IR64.

Key words: spotted-leaf mutant, bacterial blight resistance, gene mapping, Oryza sativa L.

摘要：

通过双环氧丁烷 (diepoxybutane)诱变籼稻品种IR64获得遗传稳定的水稻褐色斑点叶突变体hm197。在自然条件下,该突变体褐色斑点自播种后10周开始于叶尖出现,而后慢慢扩散至全叶。遗传分析表明,该褐色斑点性状受一对隐性核基因控制,暂名spl^hm¹⁹⁷,并将其定位在第4染色体长臂上140 kb的区段内。与野生型IR64相比,突变体株高、结实率和千粒重等农艺性状均显著下降。遮光处理表明,hm197褐色斑点的形成受自然光照的诱导。此外,hm197光合色素含量和光合效率也比野生型显著降低。组织化学分析表明,突变体中有过氧化氢和大量超氧阴离子O_{2 ?}的沉积。与IR64相比,hm197叶片中清除氧自由基酶系统中SOD和APX活性极显著上升,其余均极显著下降,同时伴随总可溶蛋白含量下降以及MDA含量上升,hm197表现出早衰迹象。抗病性鉴定表明,与野生型相比突变体对白叶枯病菌的抗性显著增强。

关键词: 斑点叶突变体, 白叶枯病抗性, 基因定位, 水稻

CLC Number:

Q343.5
Q754

Xiao-hong LI, Yong-feng SHI, Xiao-bo ZHANG, Bao-hua FENG, Li-xin SONG, Hui-mei WANG, Xia XU, Qi-na HUANG, Dan GUO, Jian-li WU. Identification and Gene Mapping of a Spotted-leaf Mutant hm197 in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(5): 447-456.

李小红, 施勇烽, 张晓波, 奉保华, 宋莉欣, 王惠梅, 徐霞, 黄奇娜, 郭丹, 吴建利. 水稻斑点叶突变体hm197的鉴定及其基因定位[J]. 中国水稻科学, 2015, 29(5): 447-456.

Figures/Tables 11

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材料 Material	株高 Plant height /cm	有效穗数 No.of productive panicles	穗长 Panicle length /cm	每穗实粒数 Filled grain number per panicle	结实率 Seed-setting rate /%	千粒重 1000-grain weight /g
hm197	90.2 ± 0.9^**	24 ± 1.0	23.4 ± 0.5	68.7 ± 7.6^**	70.2 ± 1.3^**	18.8 ± 0.5^**
IR64	113.6 ± 0.3	22 ± 1.0	25.0 ± 1.0	102.2 ± 17.3	85.2 ± 3.2	24.4 ± 1.1

材料 Material	株高 Plant height /cm	有效穗数 No.of productive panicles	穗长 Panicle length /cm	每穗实粒数 Filled grain number per panicle	结实率 Seed-setting rate /%	千粒重 1000-grain weight /g
hm197	90.2 ± 0.9^**	24 ± 1.0	23.4 ± 0.5	68.7 ± 7.6^**	70.2 ± 1.3^**	18.8 ± 0.5^**
IR64	113.6 ± 0.3	22 ± 1.0	25.0 ± 1.0	102.2 ± 17.3	85.2 ± 3.2	24.4 ± 1.1

播种后周数 Weeks after sowing	材料 Material	叶绿素a含量 Chlorophyll a content /(mg·g^-1)	叶绿素b含量 Chlorophyll b content /(mg·g^-1)	叶绿素a/b Chlorophyll a /Chlorophyll b	类胡萝卜含量 Carotenoid content /(mg·g^-1)
3	hm197	3.05 ± 0.13	0.80 ± 0.06	3.81 ± 0.07	0.64 ± 0.05
	IR64	2.93 ± 0.15	0.75 ± 0.03	3.90 ± 0.12	0.65 ± 0.03
10	hm197	2.29 ± 0.07^**	0.70 ± 0.01^**	3.28 ± 0.09	0.48 ± 0.01^**
	IR64	3.13 ± 0.05	0.92 ± 0.02	3.41 ± 0.05	0.65 ± 0.01
17	hm197	1.82 ± 0.17^**	0.56 ± 0.06^**	3.27 ± 0.09^**	0.42 ± 0.04^**
	IR64	3.44 ± 0.08	1.16 ± 0.04	2.97 ± 0.07	0.65 ± 0.03

播种后周数 Weeks after sowing	材料 Material	叶绿素a含量 Chlorophyll a content /(mg·g^-1)	叶绿素b含量 Chlorophyll b content /(mg·g^-1)	叶绿素a/b Chlorophyll a /Chlorophyll b	类胡萝卜含量 Carotenoid content /(mg·g^-1)
3	hm197	3.05 ± 0.13	0.80 ± 0.06	3.81 ± 0.07	0.64 ± 0.05
	IR64	2.93 ± 0.15	0.75 ± 0.03	3.90 ± 0.12	0.65 ± 0.03
10	hm197	2.29 ± 0.07^**	0.70 ± 0.01^**	3.28 ± 0.09	0.48 ± 0.01^**
	IR64	3.13 ± 0.05	0.92 ± 0.02	3.41 ± 0.05	0.65 ± 0.01
17	hm197	1.82 ± 0.17^**	0.56 ± 0.06^**	3.27 ± 0.09^**	0.42 ± 0.04^**
	IR64	3.44 ± 0.08	1.16 ± 0.04	2.97 ± 0.07	0.65 ± 0.03

播种后周数 Weeks after sowing	材料 Material	P_n /(μmol· m^-2· s^-1)	T_r /(mmol·m^-2·s^-1)	G_s /(mol·m^-2s^-1)	C_i /(μmol·mol^-1)
9	hm197	24.80 ± 0.96^**	3.19 ± 0.08	0.066 ± 0.002	311.58 ± 21.51^**
	IR64	28.06 ± 0.60	3.19 ± 0.06	0.069 ± 0.001	365.80 ± 26.38
10	hm197-spl	15.63 ± 0.78^**	2.43 ± 0.07^**	0.047 ± 0.001^**	243.10 ± 37.43^**
	hm197-no	22.64 ± 0.66^**	2.60 ± 0.21^**	0.053 ± 0.005^**	339.31 ± 14.23^**
	IR64	24.38 ± 0.97	2.93 ± 0.10	0.059 ± 0.002	412.28 ± 19.88
17	hm197-fl	10.19 ± 1.32^**	2.09 ± 0.28^**	0.046 ± 0.007^**	19.79 ± 4.46^**
	hm197-sul	12.03 ± 1.06^**	2.36 ± 0.15^**	0.053 ± 0.004^**	36.17 ± 4.07^**
	IR64-fl	23.08 ± 1.67	3.09 ± 0.08	0.077 ± 0.003	172.11 ± 1.19
	IR64-sul	16.88 ± 1.16	2.78 ± 0.20	0.064 ± 0.005	107.55 ± 4.72