中国水稻科学

中国水稻科学

• 研究报告 •    下一篇

我国主要杂交水稻组合及其亲本SSR标记和纯度鉴定

彭锁堂1,3; 庄杰云2; 颜启传1; 郑康乐2   

  1. 1浙江大学 农业与生物技术学院， 浙江 杭州 310029； 2中国水稻研究所 国家水稻改良中心, 浙江 杭州 310006; 3浙江森禾种业股份有限公司， 浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-01-10 发布日期:2003-01-10

SSR Markers Selection and Purity Detection of Major Hybrid Rice Combinations and Their Parents in China

PENG Suo-tang 1; ZHUANG Jie-yun 2; YAN Qi-chuan 1; ZHENG Kang-le 2   

  1. （1 College of Agricultural and Biotechnology; Zhejiang University; Hangzhou 310029; China; 2Chinese National Center for Rice Improvement; China National Rice Research Institute; Hangzhou 310006; 3Zhejiang Senhe Seed Co.; Ltd; Hangzhou 310021; China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-01-10 Published:2003-01-10

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摘要： 选用分布于水稻12条染色体上的26对SSR引物对我国9个主要的杂交水稻组合及其亲本进行了SSR标记分析，21对多态性引物共扩增出62条条带，平均2.95条，能够有效地区分所有恢复系和大部分不育系。杂交种条带均为父母本的互补型，很适合做杂交种纯度鉴定。用引物RM17对杂交稻组合汕优63和两优培九进行了100粒单种子SSR鉴定，所测纯度分别为96.0%和98.0%，与田间纯度96.2%和97.7%非常接近，显示出SSR技术在品种认证和纯度鉴定中有广阔的前景。

关键词: 杂交水稻, 遗传标记, 纯度, 鉴定

Abstract: Twenty six SSR primers distributing in 12 rice chromosomes were used to detect nine major hybrid rice combinations and their parents in China. Out of the primers used, 21 were polymorphic and amplified 62 bands totally with a mean of 2.95 bands per primer. It could distinguish all restorer lines and most sterile lines effectively. The bands of hybrid rice were complement types of their parents, which were adapted to examine purity of hybrid rice seed. Primer RM17 was selected for SSR identification with 100 single seeds of Shanyou 63 and Liangyoupeijiu. The purity was 96.0% and 98.0%, respectively, which were approximate to their field purity (96.2% and 97.7%, respectively). It suggested that SSR technique has wide prospective in variety authentication and purity identification.

Key words: hybrid rice, genetic marker, purity, identification