中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

粳稻近缘品种的SSR分析

鲍根良; 小林麻子; 富田 桂; 金庆生; 张小明; 严文潮; 叶胜海   

  1. 1浙江省农业科学院 作物与核技术利用研究所， 浙江 杭州 310021； 2日本福井县农业试验场， 日本 福井 9188215
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-01-10 发布日期:2005-01-10

Polymorphism Analysis of Closely Related japonica Rice Varieties by Using SSR Markers

Bao Gen-liang;Kobayashi Asako;Komisa Katsura;Jin Qing-sheng;Zhang Xiao-ming;Yan Wen-chao;Ye Sheng-hai   

  • Received:1900-01-01 Revised:1900-01-01 Online:2005-01-10 Published:2005-01-10

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摘要： 选用38对SSR引物，对16个日本优质米品种越光及其近缘品种进行了SSR标记分析。结果表明，有8对引物在品种间表现多态性，多态性引物检出率为21.1%，平均每对引物检测到2.25个等位基因。8个引物组合在一起，除了早喜光和日光2个品种不能区分外，其余品种均能有区别。多态性检出率亲本间为18.4%，越光及其衍生品种间为0~158%，表明近缘品种间多态性检出率较低。

关键词: 微卫星标记, 水稻, 近缘品种

Abstract: Thirty-eight SSR (simple sequence repeats) primer pairs distributing on 12 chromosomes in rice were used to analyze 16 japonica type rice varieties, which included Koshihikari and its closely related varieties. Eight of the primer pairs were polymorphic, accounting for 21.1% of the total, and 2.25 bands were detected for each primer pairs on the average. All the tested varieties except Sakihikari and Hinohikari could be distinguished. Polymorphisms detected were 18.4% between the parents of Koshihikari and 0-15.8% among Koshihikari and its closely related varieties, indicating low polymorphism among closely related varieties.

Key words: simple sequence repeats, rice, closely related varieties, polymorphism