中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

多效唑在水稻试管苗离体调控技术中的应用

赵成章；戚秀芳；郑康乐；徐星明   

  1. 中国水稻研究所，杭州310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1990-12-17 发布日期:1990-12-17

Study of Regulation Technique of Rice Plantlet in vitro with Multi-Effect Triazole

Zhao Chengzhang; Qi Xiufang; Zheng Kangle; Xu Xingming   

  • Received:1900-01-01 Revised:1900-01-01 Online:1990-12-17 Published:1990-12-17

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被引次数

20

摘要： 研究了植物生长延缓剂多效唑在组织培养中对水稻愈伤组织诱导、分化以及壮苗培养的影响。结果表明：(1)多效唑(MET)能抑制未成熟胚愈伤组织的诱导率而能明显地提高愈伤组织的绿苗形成率，以MET 2．5 mg／l时效果较好；(2)MET明显地抑制再生苗地上部生长而促进根系的发育，这种抑制效果通常保持2个月左右，不过MET对叶龄的影响不大；(3)MET处理的再生苗移栽后成活率为95％左右而且生长健壮；(4)与对照相比，MET处理的再生苗具有较大的维管束，较厚的叶肉细胞、致密的表皮细胞，并在维管束细胞中有较多的营养物质。

关键词: 多效唑, 未成熟胚, 愈伤组织诱导, 组织培养, 水稻

Abstract: The results indicated that: (1) Multi-Effect Triazole (MET) inhibitcd the callus induction from immature embryo. However, the callus redifferentiation and green plantlet formation were promoted significantly, especially with lower concentration of MET (2.5mg/l); (2) MET inhibited the plant height obviously and promoted the development of the root system of plantlet, such effect usually maintained for about 2 months But the effect of MET on leafage was not significant; (3) The survival rate of MET hardened plantlets was about 95% and the plantlcts grew more vigorously after transplanting; (4) compared with the control, the MET treated plantlets contained bigger vascular, thicker mesophyll cells, denser surface cells and more inclusion in the vascular cells.

Key words: Multi-Effect Triazole, Immature embryo, Callus induction, Plant tissue culture, Rice