中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

水稻穗瘟离体接种技术研究

孙国昌1; 孙漱沅1；申宗坦2   

  1. 1 浙江省农业科学院，杭州310021；2 浙江农业大学，杭州310029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1992-03-05 发布日期:1992-03-05

A New Inoculation Technique for Neck Blast on in vitro Rice Panicle

Sun Guochang; Shun Shuyuan; Shen Zongtan   

  • Received:1900-01-01 Revised:1900-01-01 Online:1992-03-05 Published:1992-03-05

PDF

被引次数

15

关键词: 人工接种, 离体稻穗, 穗颈瘟, 稻瘟病

Abstract: A technique for inoculating with Pyricularia oryzae Cav. for neck blast resistance screening was studied. Inoculation is carried out by spraying or smearing the mixture 1-2×105 conidia/ml suspended in 2% carboxymethyl cellulose (CMC) onto the in vitro rice panicle with a brush. The new method is suitale for inoculation at 0-10 days after heading, especially at the 0-3 days after heading. The disease index after inoculation is injection > smearing > spraying two times > spraying one times > around neck with cotton soaked in spore suspension. The method requires only a small amount of spore suspension and gives a high infection frequency, when smearing inoculation was used. It is an effective method for studying the resistance resource screening and resistance inheritance of neck blast.

Key words: Artificial inoculation, In vitro rice panicle, Neck blast, Pyricularia oryzae