中国水稻科学 ›› 2022, Vol. 36 ›› Issue (2): 139-149.DOI: 10.16819/j.1001-7216.2022.210308

• 研究报告 • 上一篇    下一篇

OsESV1基因对水稻淀粉合成的影响

王永祥, 燕海刚, 徐含聪, 傅玉双, 单壮壮, 胡晓晴, 张文伟*(), 江玲   

  1. 南京农业大学 作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心, 南京 210095
  • 收稿日期:2021-03-19 修回日期:2021-05-12 出版日期:2022-03-10 发布日期:2022-03-11
  • 通讯作者: 张文伟
  • 基金资助:
    国家转基因生物新品种培育重大专项(2016ZX08001006);国家重点研发计划资助项目(2016YFD0100101-08)

Effects of OsESV1 on Starch Synthesis in Rice

WANG Yongxiang, YAN Hangang, XU Hancong, FU Yushuang, SHAN Zhuangzhuang, HU Xiaoqing, ZHANG Wenwei*(), JIANG Ling   

  1. State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2021-03-19 Revised:2021-05-12 Online:2022-03-10 Published:2022-03-11
  • Contact: ZHANG Wenwei

摘要:

【目的】 研究水稻EARLY STARVATION1 (OsESV1)基因对水稻淀粉代谢的影响。【方法】 通过CRISPR-Cas9技术获得osesv1突变体,考查osesv1的表型及胚乳淀粉的理化特性,分析OsESV1的表达特性及相关功能。【结果】 OsESV1蛋白在植物界中十分保守。osesv1突变体株高、穗长、每穗粒数低于野生型,分蘖数显著多于野生型,叶片中淀粉含量显著下降,籽粒中的直链淀粉含量上升,而总淀粉含量无明显变化。OsESV1呈组成型表达,并且具有昼夜节律表达的特征。OsESV1蛋白定位在叶绿体内且呈点状分布。酵母双杂交和双分子荧光互补实验结果表明OsESV1蛋白可以与ADP-葡萄糖焦磷酸化酶小亚基(OsAGPS) 2a和OsAGPS1互作。【结论】 OsESV1基因影响水稻叶片的淀粉合成途径,而对胚乳淀粉合成的影响不明显。

关键词: 水稻, 淀粉, 胚乳, OsESV1, 互作

Abstract:

【Objective】 It is important to reveal the role of EARLY STARVATION1 (OsESV1) in starch metabolism of rice.【Method】 The osesv1 mutants were obtained by using the CRISPR-Cas9 system. The phenotype and the physicochemical properties of endosperm starch of osesv1 were investigated. The expression pattern and function of OsESV1 were analyzed. 【Result】 The OsESV1 protein was conserved in plants. Compared with the wild type, the osesv1 mutants exhibited lower plant height, panicle length, and grain number per panicle, but higher tiller number. Though the starch contents in osesv1 leaves significantly decreased, the total starch contents of osesv1 grains did not obviously altered and the amylose contents increased. The expression of OsESV1 was constitutive and rhythmic. OsESV1 was located in chloroplasts with a dot-like distribution. Yeast two-hybrid and bimolecular fluorescence complementation(BiFC) assays showed that OsESV1 could interact with ADP glucose pyrophosphorylase small subunit (OsAGPS) 2a and OsAGPS1.【Conclusion】 OsESV1 significantly affects the starch synthesis pathway in rice leaves, while not in endosperm.

Key words: rice, starch, endosperm, OsESV1, interaction