水稻迟抽穗突变体dth9的遗传分析与基因定位

doi:10.16819/j.1001-7216.2016.5156

中国水稻科学 ›› 2016, Vol. 30 ›› Issue (3): 232-238.DOI: 10.16819/j.1001-7216.2016.5156

水稻迟抽穗突变体dth9的遗传分析与基因定位

叶卫军^1,², 胡时开^2,³, 吴立文², 郭龙彪², 钱前^2,^3,^*()

¹浙江大学农业与生物技术学院, 杭州 310058
²中国水稻研究所, 杭州 310006
³中国农业科学院深圳农业基因组研究所, 广东深圳 518120

收稿日期:2015-10-26 修回日期:2015-12-19 出版日期:2016-05-10 发布日期:2016-05-10
通讯作者: 钱前
作者简介:
# 共同第一作者;
基金资助:
国家自然科学基金资助项目(31501279,31521064,31271700);国家973计划资助项目(2013CBA01405);中国博士后科学基金资助项目(2015M570181);深圳市科技计划资助项目(JCYJ20150630165133402)

Genetic Analysis and Gene Mapping of a Heading-delayed Mutant dth9 in Rice (Oryza sativa L.)

Wei-jun YE^1,², Shi-kai HU^2,³, Li-wen WU², Long-biao GUO², Qian QIAN^2,^3,^*()

¹ College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China
²China National Rice Research Institute, Hangzhou 310006, China
²Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China

Received:2015-10-26 Revised:2015-12-19 Online:2016-05-10 Published:2016-05-10
Contact: Qian QIAN
About author:
# These authors contributed equally to this work;

摘要/Abstract

摘要：

在EMS诱变的93-11突变体库中筛选到一个稳定遗传的迟抽穗突变体dth9 (days to heading 9)。该突变体的抽穗期比野生型延长了50d左右,其他农艺性状基本无异。遗传分析表明迟抽穗性状受一个隐性核基因控制。以突变体dth9与日本晴和武运粳7号杂交构建的F₂分离群体作为定位群体,利用SSR标记和新开发的8个InDel标记,将DTH9定位在第9染色体着丝粒附近D9-9和D9-17之间240 kb的区间内,该区域尚未发现与抽穗期有关的基因。此外,实时荧光定量PCR结果表明,在突变体dth9中与抽穗期相关基因的表达量显著降低。

关键词: 水稻, 抽穗期, 遗传分析, 基因定位

Abstract:

A heading-delayed mutant, dth9 (days to heading 9)was identified from an ethyl methylsulfonate (EMS)-induced 93-11 mutant library. The heading date of dth9 delayed about 50 days compared to the wild-type and there was no significant difference in other agronomic traits. Genetic analysis showed that the phenotype of dth9 was controlled by a single recessive nuclear gene. To map this gene, two F₂populations were generated by crossing the dth9 mutant with Nipponbare or Wuyunjing 7 as mapping populations. By using SSR markers and eight new designed InDel markers, DTH9 was narrowed to a 240kb interval between the markers D9-9 and D9-17 near the centromere of chromosome 9, there were no reports about genes associated with heading date in this interval. In addition, the expression levels of genes related to heading date were significantly decreased in dth9 by quantitative real-time PCR analysis.

Key words: rice, heading date, genetic analysis, gene mapping

中图分类号:

叶卫军, 胡时开, 吴立文, 郭龙彪, 钱前. 水稻迟抽穗突变体dth9的遗传分析与基因定位[J]. 中国水稻科学, 2016, 30(3): 232-238.

Wei-jun YE, Shi-kai HU, Li-wen WU, Long-biao GUO, Qian QIAN. Genetic Analysis and Gene Mapping of a Heading-delayed Mutant dth9 in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2016, 30(3): 232-238.

图/表 8

参考文献 25

[1]	刘广林, 罗群昌, 陈远孟, 等. 水稻种质资源抽穗扬花期耐冷性鉴定评价. 西南农业学报, 2013, 26(2): 395-398.
	Liu G L, Luo Q C, Chen Y M, et al.Analysis and evaluation on cold tolerance for rice germplasm resources at flowering stage.Southwest China J Agric Sci, 2013, 26(2): 395-398.(in Chinese with English abstract)
[2]	邱磊, 蒋海潮, 冯玉涛, 等.控制水稻抽穗期和株高的QTL定位及遗传分析. 基因组学与应用生物学, 2014, 33(4): 828-835
	Qiu L, Jiang H C,Feng Y T, et al.Mapping and genetic analycis of QTL for heading date and plant height in rice.Genonm Appl Biol,2014,33(4):828-835.(in Chinese with English abstract)
[3]	邓晓建, 周开达, 李仁端, 等. 水稻完全显性早熟性的发现和基因定位. 中国农业科学, 2001, 34(3): 233-239.
	Deng X J, Zhou K D, Li R D, et al.Identification and gene mapping of completely dominant earliness in rice (Oryza sativa L.).Sci Agric Sin, 2001, 34(3): 233-239.(in Chinese with English abstract)
[4]	胡时开, 苏岩, 叶卫军, 等. 水稻抽穗期遗传与分子调控机理研究进展. 中国水稻科学, 2012, 26(3): 373-382.
	Hu S K, Sun Y, Ye W J, et al.Advances in genetic analysis and molecular regulation mechanism of heading date in rice (Oryza sativa L.).Chin J Rice Sci, 2012, 26(3): 373-382.(in Chinese with English abstract)
[5]	Yano M, Katayose Y, Ashikari M, et al.Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS,Plant Cell, 2000, 12(12): 2473-2484.
[6]	Kojima S, Takahashi Y, Kobayashi Y, et al.Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions.Plant Cell Physiol, 2002, 43(10): 1096-1105.
[7]	Doi K, Izawa T, Fuse T, et al.Ehd1, a B-type response regulator in rice, confers short-day promotion of flowering and controls FT-like gene expression independently of Hd1.Genes & Dev, 2004, 18(8): 926-936.
[8]	Takahashi Y, Shomura A, Sasaki T, et al.Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2.Proc Natl Acad Sci USA, 2001, 98(14): 7922-7927.
[9]	Matsubara K, Yamanouchi U, Nonoue Y, et al.Ehd3, encoding a plant homeodomain finger-containing protein, is a critical promoter of rice flowering.Plant J, 2011, 66(4): 603-612.
[10]	Gao H, Zheng X M, Fei G, et al.Ehd4 encodes a novel and Oryza-genus-specific regulator of photoperiodic flowering in rice.Plos Genet, 2013, 9(2): e1003281.
[11]	Xue W, Xing Y, Weng X, et al.Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice.Nat Genet, 2008, 40(6): 761-767.
[12]	Wei X, Xu J, Guo H, et al.DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously.Plant Physiol, 2010, 153(4): 1747-1758.
[13]	Wu K S, Tanksley S D.Abundance, polymorphism and genetic mapping of microsatellites in rice.Mol Gen Genet, 1993, 241(1): 225-235.
[14]	Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method.Methods, 2001, 25: 402-408.
[15]	Hirochika H, Guiderdoni E, An G, et al.Rice mutant resources for gene discovery.Plant Mol Biol, 2004, 54(3): 325-334.
[16]	郭梁, 张振华, 庄杰云. 水稻抽穗期QTL及其与产量性状遗传控制的关系. 中国水稻科学, 2012, 26(2): 235-245.
	Guo L, Zhang Z H, Zhuang J Y.Quantiative trait loci for heading date and their relationship with the genetic control of yield traits in rice (Oryza sativa).Chin J Rice Sci, 2012, 26(2): 235-245.(in Chinese with English abstract)
[17]	Thomson M J, Edwards J D, Septiningsih E M, et a1. Substitution mapping of dth1.1, a flowering-time quantitative trait locus (QTL) associated with transgressive variation in rice, reveals multiple sub-QTL.Genetics, 2006, 172: 2501-2514.
[18]	Lin H, Ashikari M, Yamanouchi U, et a1. Identification and characterization of a quantitative trait locus, Hd9, controlling heading date in rice.Breeding Sci, 2002, 52(1): 35-41.
[19]	Takeuchi Y, Lin S Y, Sasaki T, et a1. Fine linkage mapping enables dissection of closely linked quantitative trait loci for seed dormancy and heading in rice.Theor Appl Genet, 2003, 107(7): 1174-1180.
[20]	Matsubara K, Kono I, Hori K, et a1. Novel QTLs for photoperiodic flowering revealed by using reciprocal backcross inbred lines from crosses between japonica rice cultivars.Theor Appl Genet, 2008, 117(6): 935-945.
[21]	Monna L, Lin H X, Kojima S, et a1. Genetic dissection of a genomic region for a quantitative trait locus, Hd3, into two loci, Hd3a and Hd3b, controlling heading date in rice.Theor Appl Genet, 2002, 104(5): 772-778.
[22]	Lin H, Liang Z W, Sasaki T, et a1. Fine mapping and characterization of quantitative trait loci Hd4 and Hd5 controlling heading date in rice.Breeding Sci, 2003, 53(1): 51-59.
[23]	Yamamoto T, Kuboki Y, Lin S Y, et a1. Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors.Theor Appl Genet, 1998, 97(1): 37-44.
[24]	Yan W H, Wang P, Chen H X, et al.A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice.Mol Plant, 2011, 4(2): 319-330.
[25]	杜雪树, 戚华雄, 廖世勇, 等.水稻抽穗期分子生物学研究进展. 湖北农业科学, 2013,52(24): 5958-5962.
	Du X S,Qi H X,Liao S Y, et al.Advances on the molecular biology of rice heading date.Hubei Agric Sci,2013,52(24):5958-5962.(in Chinese)

分子标记 Marker	正向引物 (5'-3') Forward primer (5'-3')	反向引物 (5'-3') Reverse primer (5'-3')	大小 Size/bp	物理位置 Physical position/Mb
D9-4	AGCCTCATACCTCCCACA	CGCCTGGAAGACAATCAA	151	3.047
D9-7	AAAGATTCTCAAGGCCAGTC	TATCTAGATCGTGGCCCA	172	3.583
D9-8	TTGCATGGTCACGTTCCT	TGATTGCGGAGTGATGAG	260	3.608
D9-9	CCAATGTAGCAGCCGTAA	CGTTGAGGATTCAGTGGT	129	3.983
D9-17	AATCGGTGAATGTCCTTG	GAAACATCCATGCCTTGC	124	4.223
D9-19	TCCATCGCATTTGAGTGT	AAGTTAGTAGGCGGAAGG	223	4.332
D9-12	GGGGTGATGCTGGTTTAT	AAGGGTCTCATCTGGAAAA	255	4.354
D9-2	GGCTTCTCAACCAAGGTAA	ACGCATCAAATCAGGCAC	205	4.554
RM444	GCTCCACCTGCTTAAGCATC	TGAAGACCATGTTCTGCAGG	162	5.925

分子标记 Marker	正向引物 (5'-3') Forward primer (5'-3')	反向引物 (5'-3') Reverse primer (5'-3')	大小 Size/bp	物理位置 Physical position/Mb
D9-4	AGCCTCATACCTCCCACA	CGCCTGGAAGACAATCAA	151	3.047
D9-7	AAAGATTCTCAAGGCCAGTC	TATCTAGATCGTGGCCCA	172	3.583
D9-8	TTGCATGGTCACGTTCCT	TGATTGCGGAGTGATGAG	260	3.608
D9-9	CCAATGTAGCAGCCGTAA	CGTTGAGGATTCAGTGGT	129	3.983
D9-17	AATCGGTGAATGTCCTTG	GAAACATCCATGCCTTGC	124	4.223
D9-19	TCCATCGCATTTGAGTGT	AAGTTAGTAGGCGGAAGG	223	4.332
D9-12	GGGGTGATGCTGGTTTAT	AAGGGTCTCATCTGGAAAA	255	4.354
D9-2	GGCTTCTCAACCAAGGTAA	ACGCATCAAATCAGGCAC	205	4.554
RM444	GCTCCACCTGCTTAAGCATC	TGAAGACCATGTTCTGCAGG	162	5.925

分子标记 Marker	正向引物 (5'-3') Forward primer (5'-3')	反向引物 (5'-3') Reverse primer (5'-3')
Ghd7	AGGTGCTACGAGAAGCAAATCC	GGGCCTCATCTCGGCATAG
Ghd8	CGTGCAATGGTTTAGACTAAAG	AACAGCATCAGCATCAACAA
Hd6	ACGTGAAGCTATGGCACATC	TGTGGTCGTGCTCTGCTATT
Hd3a	GCTAACGATGATCCCGAT	CCTGCAATGTATAGCATGC
Hd1	CGTTTCGCCAAGAGATCAG	AGATAGAGCTGCAGTGGAGAAC
RFT1	CGTCCATGGTGACCCAACA	CCGGGTCTACCATCACGAGT
Ehd1	AATCGATTCCAACAACAAGCAA	TGTCGAGAGCGGTGGATGA
OsMADS51	GTCGGCAAGCTCTACGAGTACTC	GCGAATTGCTGATAGCGATCA
OsActin1	GCTATGTACGTCGCCATCCA	GGACAGTGTGGCTGACACCAT

分子标记 Marker	正向引物 (5'-3') Forward primer (5'-3')	反向引物 (5'-3') Reverse primer (5'-3')
Ghd7	AGGTGCTACGAGAAGCAAATCC	GGGCCTCATCTCGGCATAG
Ghd8	CGTGCAATGGTTTAGACTAAAG	AACAGCATCAGCATCAACAA
Hd6	ACGTGAAGCTATGGCACATC	TGTGGTCGTGCTCTGCTATT
Hd3a	GCTAACGATGATCCCGAT	CCTGCAATGTATAGCATGC
Hd1	CGTTTCGCCAAGAGATCAG	AGATAGAGCTGCAGTGGAGAAC
RFT1	CGTCCATGGTGACCCAACA	CCGGGTCTACCATCACGAGT
Ehd1	AATCGATTCCAACAACAAGCAA	TGTCGAGAGCGGTGGATGA
OsMADS51	GTCGGCAAGCTCTACGAGTACTC	GCGAATTGCTGATAGCGATCA
OsActin1	GCTATGTACGTCGCCATCCA	GGACAGTGTGGCTGACACCAT

农艺性状 Agronomic trait	野生型 Wild-type	突变体 Mutant
株高Plant height /cm	118.67±1.52	115.30±0.58^*
穗长Panicle length/cm	23.05±0.91	21.33±1.15
有效穗数 No. of effective panicles	8.3±0.5	8.0±0.8
抽穗期 Heading date/d	98.0±0.8	147.3±2.5^**
每穗实粒数 No. of filled grains per panicle	171.5±4.0	173.0±2.7
结实率 Seed-setting rate/%	93.72±0.01	92.20±0.01
千粒重 1000-grain weight/g	31.50±0.57	31.57±0.40
一次枝梗数 Primary rachis branch number	12.0±0.7	12.6±0.6
二次枝梗数 Secondary rachis branch number	36.8±1.3	37.3±0.6

水稻迟抽穗突变体dth9的遗传分析与基因定位

Genetic Analysis and Gene Mapping of a Heading-delayed Mutant dth9 in Rice (Oryza sativa L.)

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Metrics

组合 Cross	F₁表型 Phenotype of F₁	F₂			χ²
组合 Cross	F₁表型 Phenotype of F₁	正常植株数 No. of normal plants		迟抽穗植株数 No. of heading- delayed plants	总数 Total
DTH9/93-11	正常抽穗期 Normal	662	208	870	0.281
93-11/DTH9	正常抽穗期 Normal	389	119	508	0.343

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