中国水稻科学

中国水稻科学

• 研究报告 •    下一篇

水稻端四体的分子细胞学鉴定及染色体行为分析

龚志云;高清松;于恒秀;裔传灯;顾铭洪   

  1. 扬州大学 江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室， 江苏 扬州 225009
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-10 发布日期:2008-07-10

Molecular-Cytological Identification and Chromosome Behavior Analysis of Telotetrasomic in Rice

GONG Zhi-yun; GAO Qing-song; YU Heng-xiu; YI Chuan-deng; GU Ming-hong   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-10 Published:2008-07-10

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被引次数

5

摘要： 在水稻品种中籼3037第9染色体短臂端三体的自交后代中发现了一个变异株。该植株叶片内卷,株型偏散，结实率差。分子细胞学鉴定表明，该植株体细胞染色体数比正常植株多2条，多出的染色体均比较小。进一步用来源于水稻着丝粒特异DNA序列（RCS2）以及位于水稻第9染色体短臂上的特异性DNA序列为探针，进行荧光原位杂交分析，证明该变异株多出的染色体均为第9染色体短臂，故该变异株为第9染色体短臂端四体。对变异株的减数分裂染色体行为进行分析表明，在所观察的25个细胞中，96%的细胞增加的两个端着丝粒染色体可配对形成二价体，一般不与正常的第9染色体配对形成多价体。但额外染色体形成的二价体在中期Ⅰ容易发生提前解离的现象。

关键词: 水稻, 端四体, 减数分裂, 荧光原位杂交, 分子细胞学, 染色体行为

Abstract: From the progenies of telotrisomic chromosome 9 of an indica variety, Zhongxian 3037, a phenotypical mutant was selected. Cytological and molecular cytological investigations revealed two extra chromosomes, which were the shortest in somatic cells of the mutant. Fluorescent in situ hybridization (FISH) using a rice centromere specific DNA(RCS2) and DNA sequence specific for chromosome 9 on premetaphase and pachytene chromosomes showed that these two chromosomes were the short arms of chromosome 9. That is to say, the mutant was a telotetrasomic of chromosome 9. Among the 25 pachytene cells, two telosomic chromosomes paired each other as bivalents and didn’t pair with other normal chromosome 9 as multivalents in 96% cells. However, the bivalent was easy to disassociate in advance.

Key words: telotetrasomic, rice, meiosis, fluorescent in situ hybridization, molecular cytology, chromosome behavior