Application of Functional Marker to Identify Genes for Bacterial Blight Resistance in Oryza rufipogon
XIA Zhi-hui1,2,#, HAN Fei1,#, GAO Li-fen2, YUAN Qian-hua1, ZHAI Wen-xue2, LIU Di3, LUO Yue-hua1,*
1College of Agriculture, Hainan University, Haikou 570228, China; 2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3Institute of Food Crops, Hainan Academy of Agricultural Sciences, Haikou 571100, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: firstname.lastname@example.org
Field resistance of nine accessions of common wild rice （Oryza rufipogon Griff.) and a highly susceptible variety IR24 was evaluated with nine strains of Xanthomonas oryzae pv. oryzae from the Philippines. The result showed that IR24 was highly susceptible to all of the strains and six common wild rice materials resisted total nine strains, accounting for 67% of the total. Two materials from Yulin, Guangxi Zhuang Autonomous Region and Wanning, Hainan Province were susceptible to the strains PXO280 and PXO71, respectively, and a material from Gaozhou, Guangdong Province was susceptible to the strains PXO79, PXO99 and PXO339, but they were resistant to the other strains. It is concluded that there was at least one resistance gene in every material. PCR detection for the presence of cloned resistance genes with the functional markers for xa5, xa13, Xa21 and Xa27 revealed that there were no xa5 and Xa21 in all of these materials. Four materials contained recessive resistance gene xa13, which were all heterozygous. There were five homozygotes with dominant resistance gene Xa27 and three homozygotes harboring the recessive gene xa27 and one material with neither xa27 nor Xa27.
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