中国水稻科学
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中国水稻科学  2009, Vol. 23 Issue (6): 645-652     DOI: 10.3969/j.issn.1001-7216.2009.06.14
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灰飞虱对氟虫腈抗性风险评估、遗传分析及杀虫剂敏感性研究
彭永强1,高聪芬1,马崇勇2,毛玉霞1,沈晋良1,*
1南京农业大学 植物保护学院 农药科学系/农业部作物病虫害监测与防控重点开放实验室, 江苏 南京210095; 2内蒙古自治区草原工作站, 内蒙古 呼和浩特 010020; *通讯联系人, E-mail: jlshen@njau.edu.cn
Risk Assessment and Genetic Analysis of Fipronil Resistance and Insecticide Susceptibility in the Small Brown Planthopper, Laodelphax striatellus (Homoptera: Delphacidae)
PENG Yong-qiang1, GAO Cong-fen1, MA Chong-yong2, MAO Yu-xia1, SHEN Jin-liang1,*
1Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, China; 2Inner Mongolia Rangeland Station, Huhhot 010020, China; *Corresponding author, E-mail: jlshen@njau.edu.cn
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摘要 在室内采用稻茎浸渍法进行了灰飞虱对氟虫腈抗性风险评估、抗性遗传分析和杀虫剂的敏感性研究。于2005年采自无锡麦田的灰飞虱种群在室内饲养43代期间用氟虫腈筛选了41代,结果此灰飞虱种群的抗性从8.4倍上升到2305倍。根据Tabashnik介绍的方法计算,现实遗传力(h2)分别为0.0388(1~31代)、0.2636(32~43代)和0.1113(1~43代), 表明灰飞虱对氟虫腈具有一定的抗性风险。采用稻茎浸渍法测定了2007-2008年江苏无锡和浙江长兴两地灰飞虱种群对15种杀虫剂的敏感性, 结果表明,苯基吡唑类杀虫剂氟虫腈、丁烯氟虫腈和乙虫腈的毒力最高(LC50=0.2~1.7 mg/L),其次为烯啶虫胺、噻虫嗪、毒死蜱、吡蚜酮(LC50=1.7~9.7 mg/L),其余杀虫剂的毒力较低;按照年度间敏感性变化,发现2008年无锡灰飞虱种群对氟虫腈和乙虫腈的敏感性比2007年降低了1.1倍。近年来氟虫腈已广泛用于防治这种害虫,但乙虫腈几乎没有使用。因此,对氟虫腈敏感性降低的大田灰飞虱种群似乎对乙虫腈存在交互抗性。通过抗(R)、感(S)亲本、正反交(F1、 F1′)、自交(F2)及回交(BC)后代对氟虫腈的剂量反应研究了灰飞虱对氟虫腈的抗性遗传特性,结果表明,其抗性为常染色体的不完全显性遗传\[D(F1)=0.20, D(F1′)=0.38\],抗性由2个或2个以上等位基因控制。还对灰飞虱的抗性治理进行了讨论。
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彭永强
高聪芬
马崇勇
毛玉霞
沈晋良
关键词灰飞虱   杀虫剂   抗性遗传   杀虫剂敏感性     
AbstractRisk assessment and genetic analysis on fipronil resistance and insecticide susceptibility in the small brown planthopper were conducted in laboratory by rice stem dipping method. After 41generation selection with fipronil in 43 generations, the resistance level of the small brown planthopper (a population of L. striatellus from the wheat fields in Wuxi City, Jiangsu Province in 2005) to fipronil increased from 8.4 to 230.5fold compared with the susceptible strain, and the realized heritability (h2) of resistance at different selection stages according to the method described by Tabashnik was estimated as 00388 (F1 to F31), 0.2636 (F32 to F43) and 0.1113(F1 to F43), respectively. It is suggested that the L. striatellus had the definite risk of resistance to fipronil. The susceptibilities to 15 insecticides were evaluated in two field populations of L. striatellus, collected from Wuxi City, Jiangsu Province and Changxing County, Zhejiang Province in 2007 and 2008 by the rice stem dipping method. The results showed that the highest toxic insecticides to the insect was a phenylpyrazole class of insecticides including fipronil, butylenefipronil and ethiprole(LC50: 0.2-1.7 mg/L), followed by thiamethoxam, nitenpyram, pymetrozine and chlorpyrifos (LC50: 1.7-9.7 mg/L), and toxicity of the others was lower. According to a yeartoyear variation in susceptibility, it was noted that susceptibilities to fipronil and ethiprole in L. striatellus population from Wuxi City in 2008 were reduced with a reducing rate of 1.1fold, compared with those to the two insecticides in 2007. Fipronil was used widely for controlling this insect in recent years, but ethiprole wasn′t. Therefore, it seems that the L. striatellus population whose susceptibility to fipronil was reduced had crossresistance to ethiprole. Inheritance characteristic of resistance to fipronil in L. striatellus was studied through assaying doseresponse data of the resistant (R) and susceptible(S) parents, reciprocal crosses (F1, F1′), selfbred (F2) and backcross (BC) progenies to fipronil by the rice stem dipping method, and the results showed that the resistance to fipronil in L. striatellus was found to be polygenic, autosomal and inherited as a partial dominance trait \[D(F1) =0.20, D(F1′) =0.38\]. Resistance management in L. striatellus was also discussed.
Key wordsLaodelphax striatellus   insecticide   inheritance of resistance   insecticide susceptibility   resistance management   
收稿日期: 1900-01-01;
引用本文:   
彭永强,高聪芬,马崇勇等. 灰飞虱对氟虫腈抗性风险评估、遗传分析及杀虫剂敏感性研究[J]. 中国水稻科学, 2009, 23(6): 645-652 .
PENG Yong-qiang,GAO Cong-fen,MA Chong-yong et al. Risk Assessment and Genetic Analysis of Fipronil Resistance and Insecticide Susceptibility in the Small Brown Planthopper, Laodelphax striatellus (Homoptera: Delphacidae) [J]. , 2009, 23(6): 645-652 .
 
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