分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-bi

doi:10.3969/j.issn.1001-7216.2010.04.002

中国水稻科学

分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-bⁱ

姚姝^{1, 2},陈涛¹,张亚东¹,朱镇¹,赵凌¹, 赵庆勇¹,周丽慧¹,王才林^1,*

¹江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心，江苏南京 210014； ²云南农业大学农学与生物技术学院，云南昆明 650201； *通讯联系人， E-mail: clwang@jaas.ac.cn

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-10 发布日期:2010-07-10

Pyramiding of Translucent Endosperm Mutant Gene Wx-mq and Rice Stripe Disease Resistance Gene Stv-bⁱ by MarkerAssisted Selection in Rice (Oryza sativa)

YAO Shu^1,2 , CHEN Tao¹, ZHANG Ya-dong¹, ZHU Zhen¹, ZHAO Ling¹, ZHAO Qing-yong¹, ZHOU Li-hui¹, WANG Cai-lin^1，*

¹Institute of Food Crops， Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R & D Center/Nanjing Branch of Chinese National Center for Rice Improvement， Nanjing 210014, China; ² College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China; *Corresponding author, E-mail: clwang@jaas.ac.cn

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-10 Published:2010-07-10

摘要/Abstract

摘要： 以江苏高产粳稻品种武运粳7号为母本，具有条纹叶枯病抗性基因Stvbi和暗胚乳突变基因Wx-mq的日本粳稻品种关东194作父本配制杂交组合进行聚合育种。利用与Stv-bⁱ基因共分离的SCAR标记及与暗胚乳突变基因Wxmq紧密连锁的CAPS功能标记对其分离世代进行标记位点的检测，结合田间多代选育、抗性鉴定和籽粒胚乳外观鉴定，将条纹叶枯病抗性基因Stvbi和暗胚乳突变基因Wxmq同时转育到高产品种中，筛选、培育出优质、抗病、高产且农艺性状优良的水稻新品系宁9108。利用与水稻优质及抗病基因紧密连锁的分子标记，不仅初步建立了优质、抗病育种的分子标记辅助选择体系，也为水稻优质、抗病、高产育种提供了一种简单、快捷的选择方法和重要的中间材料。

关键词: 水稻, 暗胚乳突变基因, 抗条纹叶枯病基因, 分子标记辅助选择, 聚合育种

Abstract: A high yielding japonica rice variety, Wuyunjing 7, bred in Jiangsu Province, China as female parent was crossed with the Japanese rice variety Kanto 194, which carries the rice stripe disease resistance gene Stvbi and translucent endosperm mutant gene Wxmq. From F2 generations, a SCAR (sequence characterized amplified region) marker tightly linked with Stvbi and a CAPS (cleaved amplified polymorphic sequence) marker for Wxmq were used for markerassisted selection. Finally, a new japonica rice line, Ning 9108, with excellent agronomic traits was bred by multigenerational selection on stripe disease resistance and endosperm appearance. The utilization of the markers from genes related to rice quality and disease resistance was helpful not only for establishing a molecular markerassisted selection system of highquality and disease resistance in rice, but also for providing important intermediate materials and rapid selection method for good quality, disease resistance and high yield in rice breeding.

Key words: rice, translucent endosperm mutant gene, rice stripe disease resistance gene, markerassisted selection, pyramiding breeding

姚姝陈涛,张亚东,朱镇,赵凌, 赵庆勇,周丽慧,王才林,. 分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-bⁱ[J]. 中国水稻科学 2010,24(4): 341-347 . , DOI: 10.3969/j.issn.1001-7216.2010.04.002 .

YAO Shu,CHEN Tao,ZHANG Ya-dong,ZHU Zhen,ZHAO Ling,ZHAO Qing-yong,ZHOU Li-hui,WANG Cai-lin. Pyramiding of Translucent Endosperm Mutant Gene Wx-mq and Rice Stripe Disease Resistance Gene Stv-bⁱ by MarkerAssisted Selection in Rice (Oryza sativa) [J]. Chinese Journal of Rice Science 2010,24(4): 341-347 ., DOI: 10.3969/j.issn.1001-7216.2010.04.002 .

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分子标记辅助选择聚合水稻暗胚乳突变基因Wx-mq和抗条纹叶枯病基因Stv-bⁱ

Pyramiding of Translucent Endosperm Mutant Gene Wx-mq and Rice Stripe Disease Resistance Gene Stv-bⁱ by MarkerAssisted Selection in Rice (Oryza sativa)

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