水稻第6染色体短臂谷壳硅含量QTL的分解

doi:10.3969/j.issn.1001-7216.2010.01.03

摘要/Abstract

摘要： 报道了水稻谷壳硅含量QTL qHUS6的分解研究。针对前期定位的qHUS6区域，应用在第6染色体短臂RM587-RM19784区间分离、背景基本纯合的F2:3群体，将qHUS6分解为2个QTL；其中，qHUS61位于目标区间的上部，qHUS62位于下部。同时，筛选出在目标区间内携带更小杂合片段的3个单株。其中，2株覆盖qHUS61区间，各自交产生了1个F2:3群体，应用这2个群体将qHUS61定位于RM510和RM19417之间约147.0 kb的区间内；另1株覆盖qHUS62区间，自交产生了1个F2:3群体，从中挑选出在qHUS62区间具有不同基因型组成的5套F3株系，将qHUS62分解为qHUS62a和qHUS62b，分别位于RM19706-RM19795和RM314-RM19665区间。
关键词:

关键词: 水稻, 数量性状基因座, 剩余杂合体, 谷壳硅含量

Abstract:
The dissection of the QTL qHUS6 for hull silicon content in rice which was previously located on the short arm of rice chromosome 6 is reported. Using an F2:3 population segregated in the RM587-RM19784 region harboring qHUS6 in an isogenic background, two QTLs for hull silicon content were detected, of which qHUS61 was located in the upper region and qHUS62 in the lower region. Three plants carrying smaller heterozygous segments in the target region were selected, of which two covered the qHUS61 region and the other covered the qHUS62 region. Three F2:3 populations were derived from the selfed seeds of the three plants, respectively. QTL mapping was performed using the two populations segregated in the qHUS61 region, and qHUS61 was delimited to a 147.0 kb region flanked by RM510 and RM19417. Five groups of F3 lines with different genotypic composition in the qHUS62 region were selected from the other F2:3 population. Two QTLs were separated with twoway ANOVA, of which qHUS62a was located in the interval RM19706-RM19795 and qHUS62b in the interval RM314-RM19665.

Key words: rice (Oryza sativa), quantitative trait locus, residual heterozygous line, hull silicon content, gene mapping

吴季荣,范方军,杜景红,樊叶杨,庄杰云. 水稻第6染色体短臂谷壳硅含量QTL的分解[J]. 中国水稻科学 2010,24(1): 12-17 . , DOI: 10.3969/j.issn.1001-7216.2010.01.03 .

WU Ji-rong,FAN Fang-jun,DU Jing-hong,FAN Ye-yang,ZHUANG Jie-yun*. Dissection of QTLs for Hull Silicon Content on the Short Arm of Rice Chromosome 6[J]. Chinese Journal of Rice Science 2010,24(1): 12-17 ., DOI: 10.3969/j.issn.1001-7216.2010.01.03 .

参考文献

[1]Guo W, Hou Y L, Wang S G, et al. Effect of silicate on the growth and arsenate uptake by rice (Oryza sativa L.) seedlings in solution culture. Plant & Soil, 2005, 272: 173-181.

[2]Rodrigue F , Vale F X R, Datnoff L E, et al. Effect of rice growth stages and silicon on sheath blight development. Phytopathology, 2003, 93: 256-261.

[3]Ma J F, Yamaji N. Silicon uptake and accumulation in higher plants. Trends Plant Sci, 2006, 11: 392-397.

[4]Epstein E. Silicon. Annu Rev Plant Physiol Plant Mol Biol, 1999, 50: 641-664.

[5]Ma J F, Tamai K, Yamaji N, et al. A silicon transporter in rice. Nature, 2006, 440: 688-691.

[6]Ma J F, Yamaji N, Mitani N, et al. An efflux transporter of silicon in rice. Nature, 2007, 448: 209-213.

[7]Yamaji N, Mitani N, Ma J F. A transporter regulating silicon distribution in rice shoots. Plant Cell, 2008, 20: 1381-1389.

[8]Ma J F, Tamai T, Wissuwa M, et al. QTL analysis for Si uptake in rice. Plant Cell Physiol, 2004, 45(suppl): 86.

[9]Wu Q S, Wan X Y, Su N, et al. Genetic dissection of silicon uptake ability in rice (Oryza sativa L.). Plant Sci, 2006, 171: 441-448.

[10]Dai W M, Zhang K Q, Duan B W, et al. Genetic dissection of silicon content in different organs of rice. Crop Sci, 2005, 45: 1345-1352.

[11]Dai W M, Zhang K Q, Wu J R, et al. Validating a segment on the short arm of chromosome 6 responsible for genetic variation in the hull silicon content and yield traits of rice. Euphytica, 2008, 160: 317-324.

[12]杜景红，樊叶杨, 吴季荣，等. 水稻第6染色体短臂产量性状QTL簇的分解. 中国农业科学, 2008, 41: 439-445.

[13]鲍钱江, 樊叶杨，於于东，等. 水稻第6染色体短臂上株高QTL qDH61的精细定位. 中国水稻科学， 2009， 23（5）： 470-474.

[14]戴伟民，张克勤，段彬伍，等. 测定水稻硅含量的一种简易方法. 中国水稻科学， 2005， 19： 460-426.

[15]Zheng K L, Huang N, Bennett J, et al. PCRbased markerassisted selection in rice breeding. IRRI Discussion Paper Series No.12. Manila: International Rice Research Institute, 1995.

[16]施勇烽，应杰政，王磊，等. 鉴定水稻品种的微卫星标记筛选. 中国水稻科学， 2005， 19（5）： 195-201.

[17]Lander E S, Green P, Abrahamson J, et al. MAPMAKER: An interactive computer package for constructing primary genetic maps of experimental and natural populations. Genomics, 1987, 1: 174-181.

[18]Wang S, Basten C J, Zeng Z B. Windows QTL Cartographer 2.5. Raleigh, NC, USA： Department of Statistics, North Carolina State University, 2005.

[19]SAS Institute Inc. SAS/STAT User′s Guide. Cary, NC： SAS Institute, 1999.

[20]Thomson M J, Edwards J D, Septiningsih E M, et al. Substitution mapping of dth1.1， a floweringtime quantitative trait locus (QTL) associated with transgressive variation in rice, reveals multiple subQTL. Genetics, 2006, 172: 2501-2514.

[21]Yano M, Katayose Y, Ashikari M, et al. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell, 2000, 12: 2473-2483.