Chinese Journal OF Rice Science ›› 2016, Vol. 30 ›› Issue (3): 304-312.DOI: 10.16819/j.1001-7216.2016.5150
• Orginal Article • Previous Articles Next Articles
Zhen-hua XU1,2, Ying QU1, Hai-ying LIU1,2, Li-nan ZHU1, Zhong-chen ZHANG1, Zheng-xun JIN1,*()
Received:
2015-10-19
Revised:
2015-12-23
Online:
2016-05-10
Published:
2016-05-10
Contact:
Zheng-xun JIN
徐振华1,2, 曲莹1, 刘海英1,2, 朱立楠1, 张忠臣1, 金正勋1,*()
通讯作者:
金正勋
基金资助:
CLC Number:
Zhen-hua XU, Ying QU, Hai-ying LIU, Li-nan ZHU, Zhong-chen ZHANG, Zheng-xun JIN. Expression Characteristics and Sequence Variation Analysis of Glutamine Synthetase Gene in Grain of japonica Rice with Transgressive Variation[J]. Chinese Journal OF Rice Science, 2016, 30(3): 304-312.
徐振华, 曲莹, 刘海英, 朱立楠, 张忠臣, 金正勋. 粳稻超亲变异系籽粒谷氨酰胺合成酶基因表达特性及序列变异分析[J]. 中国水稻科学, 2016, 30(3): 304-312.
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2016.5150
引物名称 Primer name | 引物序列号 Primer sequence number | 引物序列(5'-3') Primer sequence(5'-3') |
---|---|---|
RT-GS1-3-S | AB180689 | CTCCACCCTCAAGCCATCT |
RT-GS1-3-A | ACGACGCCAGCAATCTCA | |
RT-GS2-S | X14246 | GGAACCCATCCCTACTAACA |
RT-GS2-A | TCACCTCAAATCCTCCATCT | |
Os-GS1-3-A | AB180689 | CTCACTTGCCGTTGGATT |
Os-GS1-3-S | ATTGATAGCCTGTGCGTCTC | |
Os-GS2-S | X14246 | GTTGGTGATTATCTGTAGGGG |
Os-GS2-A | AAGGTGGCGTGGTTTTCT |
Table 1 PCR primers used in the study.
引物名称 Primer name | 引物序列号 Primer sequence number | 引物序列(5'-3') Primer sequence(5'-3') |
---|---|---|
RT-GS1-3-S | AB180689 | CTCCACCCTCAAGCCATCT |
RT-GS1-3-A | ACGACGCCAGCAATCTCA | |
RT-GS2-S | X14246 | GGAACCCATCCCTACTAACA |
RT-GS2-A | TCACCTCAAATCCTCCATCT | |
Os-GS1-3-A | AB180689 | CTCACTTGCCGTTGGATT |
Os-GS1-3-S | ATTGATAGCCTGTGCGTCTC | |
Os-GS2-S | X14246 | GTTGGTGATTATCTGTAGGGG |
Os-GS2-A | AAGGTGGCGTGGTTTTCT |
供试材料 Test material | 抽穗后天数 Days after heading/d | 精米蛋白质含量 Protein content in polished rice | ||||
---|---|---|---|---|---|---|
10 | 15 | 20 | 25 | 30 | ||
系选1号 Xixuan 1 | 11.68 d | 11.04 b | 11.13 b | 9.97 b | 9.26 b | 9.52 b |
通769 Tong 769 | 12.14 c | 11.06 b | 10.68 c | 9.19 c | 8.85 c | 8.27 c |
东农1101 Dongnong 1101 | 12.47 b | 10.04 c | 8.88 d | 8.39 d | 8.44 d | 7.81 d |
东农1124 Dongnong 1124 | 12.75 a | 11.87 a | 11.78 a | 11.94 a | 10.98 a | 10.18 a |
Table 2 Comparison of protein content in rice grains during grain filling.%
供试材料 Test material | 抽穗后天数 Days after heading/d | 精米蛋白质含量 Protein content in polished rice | ||||
---|---|---|---|---|---|---|
10 | 15 | 20 | 25 | 30 | ||
系选1号 Xixuan 1 | 11.68 d | 11.04 b | 11.13 b | 9.97 b | 9.26 b | 9.52 b |
通769 Tong 769 | 12.14 c | 11.06 b | 10.68 c | 9.19 c | 8.85 c | 8.27 c |
东农1101 Dongnong 1101 | 12.47 b | 10.04 c | 8.88 d | 8.39 d | 8.44 d | 7.81 d |
东农1124 Dongnong 1124 | 12.75 a | 11.87 a | 11.78 a | 11.94 a | 10.98 a | 10.18 a |
供试材料 Material | 10DAH | 15DAH | 20DAH | 25DAH | 30DAH |
---|---|---|---|---|---|
系选1号 Xixuan 1 | 0.39 a | 0.90 b | 0.88 c | 0.78 b | 0.58 b |
通769 Tong 769 | 0.47 a | 0.95 b | 0.99 b | 0.60 c | 0.50 b |
东农1101 Dongnong 1101 | 0.38 a | 0.71 c | 0.83 c | 0.53 c | 0.54 b |
东农1124 Dongnong 1124 | 0.41 a | 1.25 a | 1.06 a | 0.97 a | 0.87 a |
Table 3 Comparison of grain glutamine synthetase activity during grain filling for parents and hybrid progenies.OD·grain-1min-1
供试材料 Material | 10DAH | 15DAH | 20DAH | 25DAH | 30DAH |
---|---|---|---|---|---|
系选1号 Xixuan 1 | 0.39 a | 0.90 b | 0.88 c | 0.78 b | 0.58 b |
通769 Tong 769 | 0.47 a | 0.95 b | 0.99 b | 0.60 c | 0.50 b |
东农1101 Dongnong 1101 | 0.38 a | 0.71 c | 0.83 c | 0.53 c | 0.54 b |
东农1124 Dongnong 1124 | 0.41 a | 1.25 a | 1.06 a | 0.97 a | 0.87 a |
Fig. 1. Expression of genes involved in GS in grains of tested parents and their derived progenies at various days after heading. DAH,Days after heading. The same as below.
供试材料 Material | 10 DAH | 15 DAH | 20 DAH | 25 DAH | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
GS1.3 | GS2 | GS1.3 | GS2 | GS1.3 | GS2 | GS1.3 | GS2 | ||||
系选1号 Xixuan 1 | 1.00 Bc | 1.00 Dd | 1.00 Aa | 1.00 Bb | 1.00 Aa | 1.00 Bb | 1.00 Cc | 1.00 Cc | |||
通769 Tong 769 | 0.98 Bc | 2.87 Bb | 0.71 Bc | 1.06 Bb | 0.96 Aa | 0.90 Bc | 1.32 Aa | 2.16 Aa | |||
东农1101 Dongnong 1101 | 1.20 Ab | 1.76 Cc | 0.83 Bb | 0.33 Cc | 0.69 Bb | 0.72 Cd | 1.15 Bb | 0.84 Dd | |||
东农1124 Dongnong 1124 | 1.35 Aa | 3.08 Aa | 0.76 Bc | 2.16 Aa | 0.97 Aa | 1.43 Aa | 1.00 Cc | 1.80 Bb |
Table 4 Relative expression levels of GS genes in parents and hybrid progenies after heading.OD·grain-1min-1
供试材料 Material | 10 DAH | 15 DAH | 20 DAH | 25 DAH | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
GS1.3 | GS2 | GS1.3 | GS2 | GS1.3 | GS2 | GS1.3 | GS2 | ||||
系选1号 Xixuan 1 | 1.00 Bc | 1.00 Dd | 1.00 Aa | 1.00 Bb | 1.00 Aa | 1.00 Bb | 1.00 Cc | 1.00 Cc | |||
通769 Tong 769 | 0.98 Bc | 2.87 Bb | 0.71 Bc | 1.06 Bb | 0.96 Aa | 0.90 Bc | 1.32 Aa | 2.16 Aa | |||
东农1101 Dongnong 1101 | 1.20 Ab | 1.76 Cc | 0.83 Bb | 0.33 Cc | 0.69 Bb | 0.72 Cd | 1.15 Bb | 0.84 Dd | |||
东农1124 Dongnong 1124 | 1.35 Aa | 3.08 Aa | 0.76 Bc | 2.16 Aa | 0.97 Aa | 1.43 Aa | 1.00 Cc | 1.80 Bb |
Fig. 4. Comparison of homology between GS1.3(A) and GS2(B) gene sequences for the tested materials. 1, Nipponbare; 2, Xixuan 1; 3, Tong 769; 4, Dongnong 1101; 5, Dongnong 1124.
功能域 Domain | 氨基酸序列位置 Position sequence | 系选1号 Xixuan 1 | 通769 Tong 769 | 东农1101 Dongnong 1101 | 东农1124 Dongnong 1124 | |
---|---|---|---|---|---|---|
起始 Start | 终止 End | |||||
谷氨酰胺合成酶ATP结合功能域 GS ATP binding domain | 239 | 255 | — | — | — | — |
谷氨酰胺合成酶催化功能域 GS catalytic domain | 105 | 357 | V | M | M →V | M →V |
谷氨酰胺合成酶铵离子集合功能域 GS beta-grasp domain | 19 | 99 | — | — | V→I | Q→R |
C→Y |
Table 6 Conserved domain analysis of the GS1.3 sequence.
功能域 Domain | 氨基酸序列位置 Position sequence | 系选1号 Xixuan 1 | 通769 Tong 769 | 东农1101 Dongnong 1101 | 东农1124 Dongnong 1124 | |
---|---|---|---|---|---|---|
起始 Start | 终止 End | |||||
谷氨酰胺合成酶ATP结合功能域 GS ATP binding domain | 239 | 255 | — | — | — | — |
谷氨酰胺合成酶催化功能域 GS catalytic domain | 105 | 357 | V | M | M →V | M →V |
谷氨酰胺合成酶铵离子集合功能域 GS beta-grasp domain | 19 | 99 | — | — | V→I | Q→R |
C→Y |
功能域 Domain | 氨基酸序列位置 Position sequence | 系选1号 Xixuan 1 | 通769 Tong 769 | 东农1101 Dongnong 1101 | 东农1124 Dongnong 1124 | |
---|---|---|---|---|---|---|
起始 Start | 终止 End | |||||
谷氨酰胺合成酶ATP结合域GS ATP binding domain | 294 | 305 | — | — | — | — |
谷氨酰胺合成酶催化功能域 GS catalytic domain | 159 | 411 | I | T | I→V T→A | I→V T→A |
谷氨酰胺合成酶铵离子集合功能域 GS beta-grasp domain | 73 | 154 | — | — | — | — |
Table 7 Conserved domain analysis of the GS2 sequence.
功能域 Domain | 氨基酸序列位置 Position sequence | 系选1号 Xixuan 1 | 通769 Tong 769 | 东农1101 Dongnong 1101 | 东农1124 Dongnong 1124 | |
---|---|---|---|---|---|---|
起始 Start | 终止 End | |||||
谷氨酰胺合成酶ATP结合域GS ATP binding domain | 294 | 305 | — | — | — | — |
谷氨酰胺合成酶催化功能域 GS catalytic domain | 159 | 411 | I | T | I→V T→A | I→V T→A |
谷氨酰胺合成酶铵离子集合功能域 GS beta-grasp domain | 73 | 154 | — | — | — | — |
[1] | Cren M, H irel B Glulamine synthetase in higher plant: Regulation of gene and protein expression from the organ to the cell.Plant Cell Physiol, 1999.40: 1187-1193. |
[2] | Hill J, Phylip L H.Bacterial aspartic proteinases.FEBS Let, 1997, 409(3): 357-360. |
[3] | Ochs G, Schoth G,Trischler M, et al.Complexity and expression of the glutamine synthetase multigene family in the amphidiploid crop Brassi canapus. Plant Mol Biol, 1999,39: 395-405. |
[4] | 金正勋, 钱春荣, 杨静,等.水稻灌浆成熟期籽粒谷氨酰胺合成酶活性变化及其与稻米品质关系的初步研究. 中国水稻科学. 2007,21(1):103-106. |
Jin Z X, Qian C R, Yang J, et al.Changes of activity of glutamine synthetase during grain filling and its influence on rice grain quality.Chin J Rice Sci, 2007,21:103-106. | |
[5] | Paulam M, Ligia M L, Isabel M S.Expression of he plastid located luta mine synt hetase of medicago t run catula.Plant Physiol, 2003, 132(1): 390-399. |
[6] | 黄星,李晓光,刘洪亮,等. 水稻籽粒蛋白质含量选择对杂交后代蛋白质含量及氮代谢关键酶活性的影响. 中国水稻科学,2009,23(6):657-660. |
Huang X, Li X G, Liu H L, et al.Effects of grain protein content selection on protein content and key enzymes activities for nitrogen metabolism in rice hybrid progenies.Chin J Rice Sci, 2009,23:657-660 | |
[7] | 莫良玉, 吴良欢, 陶勤南. 高等植物GS/GOGAT循环研究进展. 植物营养与肥料学报, 2001, 7(2): 223-231. |
Mo L Y, Wu L H, Tao Q N.Research advances on GS/GOGAT cycle in higher plants.Plant Nut Fer Sci, 2001,7(2):223-231. | |
[8] | Tabuchi M, Sugiyama K, Ishiyama K, et al.Severe reduction in growth rate and grain filling of ricemutants lacking OsGS1:1, a cytosolic glutamine synthetase1:1.Plant J, 2005, 42: 641-651. |
[9] | Tabuchi M, Abiko T, Yamaya T.Assimilation of ammonium ions andreutilization of nitrogen in rice (Oryza sativa L.).J Exp Bot, 2007, 58: 2319-2327. |
[10] | Miflin B J,Lea P J.Ammonia assimilation //The Biochemistry of Plants. New York: Academic Press,1980:169-202. |
[11] | Jacek B, Andrzej B L.Evolution of the glutamine synthetase gene in plants.Plant Sci, 2011,28:51-58. |
[12] | 朱红梅, 荣湘民, 刘强,. 等. 不同基因型水稻籽粒蛋白质含量差异的研究:Ⅰ.两个常规早稻品种籽粒蛋白质含量差异. 湖南农业大学学报,2001, 27(1): 13-16. |
Zhu H M, Rong X M, Liu Q, et al.Differences in contents of grain protein of different genotype rice varieties: Ⅰ. Differences in contents of grain protein between two varieties of early convention rice.J Hunan Agric Univ,2011,27(1):13-16. | |
[13] | Xie Z J, Jiang D, Cao W X, et al.Effects of post anthesis soil water status on the key regulatory enzymes of starch and protein accumulation in wheat grains.J Plant Physiol Mol Biol,2003, 29(4): 309-316. |
[14] | 唐湘如, 余铁桥. 密度对饲用杂交稻几种酶活性的影响及其与产量和蛋白质含量的关系. 杂交水稻,1999, (6):47-50. |
Tang X R, Yu T Q.Effects of density on activities of several enzymes in fodder hybrid rice and its relation to protein content and yield.Hybrid Rice,1999, (6):47-50. | |
[15] | 吴永生, 李新海,郝转芳,等. 玉米Gln1-4的gDNA序列、基因结构、保守功能域与等位变异. 作物学报,2009, 35(6):983-991. |
Wu Y S, Li X H, Hao Z F, et al.Genomic DNA sequence, gene structure, conserved domains, and natural alleles of Gln1-4 gene in maize.Acta Agron Sin, 2009, 35(6):983-991. | |
[16] | Reddy A.Alternative splicing of pre-messenger RNAs in plants in the genomic era.Annu Rev Plant Biol, 2007, 58: 267-294. |
[17] | Kruglyak L.The use of a genetic map of biallelic markers in linkage studies.Nat Genet, 1997, 17: 21-24. |
[1] | HUANG Yaru, XU Peng, WANG Lele, HE Yizhe, WANG Hui, KE Jian, HE Haibing, WU Liquan, YOU Cuicui. Effects of Exogenous Trehalose on Grain Filling Characteristics and Yield Formation of japonica Rice Cultivar W1844 [J]. Chinese Journal OF Rice Science, 2023, 37(4): 379-391. |
[2] | GAO Qianqing, REN Xiaojian, ZHAI Zhongbing, ZHENG Pubing, WU Yuanfen, CUI Kehui. Effects of Panicle and Bud-promoting Nitrogen Fertilizer Application on Growth of Regenerated Bud and Grain Yield of Ratoon Rice [J]. Chinese Journal OF Rice Science, 2023, 37(4): 405-414. |
[3] | MA Zhaohui, SHI Yihan, CHENG Haitao, SONG Wenwen, LU Lianji, LIU Renguang, LÜ Wenyan. Effects of Embryo Morphology and Endosperm Composition on Embryo-remaining Characteristics in Rice [J]. Chinese Journal OF Rice Science, 2023, 37(3): 265-275. |
[4] | WANG Yu, SUN Quanyi, DU Haibo, XU Zhiwen, WU Keting, YIN Li, FENG Zhiming, HU Keming, CHEN Zongxiang, ZUO Shimin. Improvement of the Resistance of Nanjing 9108 to Blast and Sheath Blight by Pyramiding Resistance Gene Pigm and Quantitative Trait Genes qSB-9TQ and qSB-11HJX [J]. Chinese Journal OF Rice Science, 2023, 37(2): 125-132. |
[5] | PEI Feng, WANG Guangda, GAO Peng, FENG Zhiming, HU Keming, CHEN Zongxiang, CHEN Hongqi, CUI Ao, ZUO Shimin. Evaluation of New japonica Rice Lines with Low Cadmium Accumulation and Good Quality Generated by Knocking Out OsNramp5 [J]. Chinese Journal OF Rice Science, 2023, 37(1): 16-28. |
[6] | WANG Shiguang, LU Zhanhua, LIU Wei, LU Dongbai, WANG Xiaofei, FANG Zhiqiang, WU Haoxiang, HE Xiuying. Generating Guangdong Simiao Rice Germplasms by Applying CRISPR/Cas9 Gene Editing and Marker-assisted Selection Technology [J]. Chinese Journal OF Rice Science, 2023, 37(1): 29-36. |
[7] | CHEN Tao, ZHAO Qingyong, ZHU Zhen, ZHAO Ling, YAO Shu, ZHOU Lihui, ZHAO Chunfang, ZHANG Yadong, WANG Cailin. Development of New Low Glutelin Content japonica Rice Lines with Good Eating Quality and Fragrance by Molecular Marker-Assisted Selection [J]. Chinese Journal OF Rice Science, 2023, 37(1): 55-65. |
[8] | YANG Taotao, ZOU Jixiang, WU Longmei, BAO Xiaozhe, JIANG Yu, ZHANG Nan, ZHANG Bin. Effect of Free Air Temperature Increase on Grain Quality of Double-cropping Rice in South China [J]. Chinese Journal OF Rice Science, 2023, 37(1): 66-77. |
[9] | WANG Yingheng, CHEN Lijuan, CUI Lili, ZHAN Shengwei, SONG Yu, CHEN Shian, XIE Zhenxing, JIANG Zhaowei, WU Fangxi, ZHUO Chuanying, CAI Qiuhua, XIE Huaan, ZHANG Jianfu. Effects of Nitrogen Rate on Photosynthesis, Yield and Grain Quality of Superior Quality Rice “Fuxiangzhan” [J]. Chinese Journal OF Rice Science, 2023, 37(1): 89-101. |
[10] | REN Weichen, CHANG Qingxia, ZHANG Yajun, ZHU Kuanyu, WANG Zhiqin, YANG Jianchang. Characteristics and Physiological Mechanism of Carbon and Nitrogen Accumulation and Translocation of japonica Rice Varieties Differing in Nitrogen Use Efficiency [J]. Chinese Journal OF Rice Science, 2022, 36(6): 586-600. |
[11] | SHI Yuliang, YANG Yong, LI Xuefei, LI Qianfeng, HUANG Lichun, ZHANG Changquan, SONG Xuetang, LIU Qiaoquan. Comparison of Grain Quality Profiles of japonica Soft Rice Varieties with Different Amylose Contents [J]. Chinese Journal OF Rice Science, 2022, 36(6): 601-610. |
[12] | CHEN Hongyang, JIA Yan, ZHAO Hongwei, QU Zhaojun, WANG Xinpeng, DUAN Yuyang, YANG Rui, BAI Xu, WANG Changcheng. Effects of Low Temperature Stress During Grain Filling on Starch Formation and Accumulation of Superior and Inferior Grains in Rice [J]. Chinese Journal OF Rice Science, 2022, 36(5): 487-504. |
[13] | JING Wenjiang, GU Hanzhu, ZHANG Xiaoxiang, WU Hao, ZHANG Weiyang, GU Junfei, LIU Lijun, WANG Zhiqin, YANG Jianchang, ZHANG Hao. Response of Grain Quality and Root Characteristics to Irrigation Methods During Mid-season indica Rice Varieties Improvement [J]. Chinese Journal OF Rice Science, 2022, 36(5): 505-519. |
[14] | LU Dandan, YONG Mingling, TAO Yu, YE Miao, ZHANG Zujian. Characteristics of Grain Protein Accumulation and Its Response to Nitrogen Level in Good Taste Rice Varieties [J]. Chinese Journal OF Rice Science, 2022, 36(5): 520-530. |
[15] | XU Yunji, TANG Shupeng, JIAN Chaoqun, CAI Wenlu, ZHANG Weiyang, WANG Zhiqin, YANG Jianchang. Roles of Polyamines and Ethylene in Grain Filling, Grain Weight and Quality of Rice [J]. Chinese Journal OF Rice Science, 2022, 36(4): 327-335. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||