Chinese Journal OF Rice Science ›› 2023, Vol. 37 ›› Issue (3): 265-275.DOI: 10.16819/j.1001-7216.2023.220809

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Effects of Embryo Morphology and Endosperm Composition on Embryo-remaining Characteristics in Rice

MA Zhaohui1, SHI Yihan1, CHENG Haitao1, SONG Wenwen1, LU Lianji1, LIU Renguang2, LÜ Wenyan1,*()   

  1. 1Agronomy College, Shenyang Agricultural University, Shenyang 110866, China
    2Benxi Agricultural Comprehensive Development Service Center,Benxi 117022, China
  • Received:2022-08-25 Revised:2022-12-26 Online:2023-05-10 Published:2023-05-16
  • Contact: *email:lwyzxr@syau.edu.cn

水稻种子胚形态与胚乳组成对稻米留胚特性的影响

马兆惠1, 石一涵1, 程海涛1, 宋文雯1, 路连吉1, 刘仁广2, 吕文彦1,*()   

  1. 1沈阳农业大学 农学院,沈阳110866
    2本溪市农业综合发展服务中心, 辽宁 本溪117022
  • 通讯作者: *email:lwyzxr@syau.edu.cn
  • 基金资助:
    国家自然科学基金资助项目(32201892);辽宁省教育厅面上项目(LJKMZ20221016);辽宁省科技重大专项(2019JH1/10200001);沈阳农业大学成果转化项目(H2017035)

Abstract:

【Objective】The embryo of embryo-remaining rice has high nutritional value and ensures the taste of rice. However, related biological mechanisms, such as the relationship among embryo-remaining characteristics, embryo traits, and endosperm components remains unclear. Therefore, it is necessary to further study the difference of embryo-remaining rate among different types of rice varieties and its influencing factors.【Method】Therefore, typical embryo-retaining and non-embryo-remaining varieties were selected from soft japonica and common rice as experimental materials to investigate rice embryo-remaining characteristics. Here, the embryo development characteristics of the two types of varieties were observed by slicing; then, apparent amylose content (AAC) and abscission pectin contents were compared. Finally, the differences in embryo-remaining rate, embryo traits and endosperm composition were studied under external cytokinin (CTK) application.【Result】Compared with non-embryo-remaining varieties, embryo-remaining soft japonica varieties and common rice had larger inner and outer embryo width, faster embryo development, large embryo volume, better grain plumpness, wider and thicker grain shape and higher abscission pectin content. After the application of CTK, the development speed of the soft rice varieties was accelerated in the middle and late stages of development, while the embryo development of the common varieties slowed down. The AAC, pectin content, embryo weight percentage and embryo-remaining rate of the two types were increased.【Conclusion】In general, apart from embryo traits, endosperm composition mainly affected the embryo-remaining characteristics of soft rice varieties, whereas grain shape was an important factor influencing the embryo-remaining characteristics of common varieties. CTK regulates the embryo-remaining rate of different types of rice varieties by influencing different traits of rice seeds. These results will lay a new theoretical basis for the breeding and cultivation regulation of high embryo-remaining rice varieties.

Key words: embryo-remaining rate, embryo development, endosperm composition, grain shape, cytokinin

摘要:

【目的】稻米留胚能够提高稻米营养品质,但稻米留胚特性与胚性状、胚乳成分的关系等相关生物学机制并不明确。因此,有必要深入研究不同类型稻米品种留胚率差异及其影响因素。【方法】选取粳稻中软米型和普通型两类典型留胚材料及非留胚材料为试材,首先切片观察两类品种籽粒胚发育特点,继而对比表观直链淀粉含量(apparent amylose content, AAC)和离层果胶含量,最后以外源细胞分裂素(cytokinin, CTK)调控,比较胚性状、胚乳组成及留胚率差异。【结果】与非留胚材料相比,软米型和普通型留胚材料具有胚内幅和外幅较大,胚发育速度快,胚体积较大的特点;籽粒充实度好,粒形更宽、厚;同时离层果胶含量也较高。外施CTK后,软米型留胚材料中后期胚发育速度加快,普通型材料胚发育减缓;两类材料的AAC、果胶含量、胚比重及留胚率均提高。【结论】除胚性状对两类品种留胚率均有明显影响外,胚乳组成主要影响软米型材料留胚特性,而粒形是影响普通型材料留胚特性的重要因素。CTK则通过影响籽粒不同性状对不同类型材料留胚率进行调控。这为后续高留胚水稻品种的培育和栽培调控提供了理论依据和指导方向。

关键词: 留胚率, 胚发育, 胚乳成分, 粒形, 细胞分裂素