Primary Study on Effect of Secreted  Proteins of Magnaporthe oryzae During  Infection

doi:10.3969/j.issn.1001-7216.2011.04.018

Abstract

Abstract: Secreted proteins during spore germination of Magnaporthe oryzaewere identified. Ungerminated spores, germinated spores and the mixture of germinated spores with germination liquid of M. oryzae (200550, 200581) were inoculated on rice seedlings, respectively. The disease indices were 1113, 3506, 4901 and 4901, 84, 627, indicating that germination liquid played a driving role in infection process. SDSPAGE amplification revealed that secreted proteins were contained. According to the results of mass spectrometer, and database query, eight genes were involved, including MGG_01305, MGG_13188, MGG_12454, etc. The results indicated that these proteins played key roles and important function during M. oryzae infection.

Key words: Magnaporthe oryzae, secreted , protein, spore germination, mass spectrometer

摘要： 鉴定了稻瘟病菌孢子萌发过程中分泌产生的蛋白质。将稻瘟病菌菌株200550、200581未萌发孢子、萌发孢子以及含有孢子萌发液的萌发孢子分别接种后，病情指数分别为11.13, 35.06, 49.01和49.6, 8.4, 62.7；说明孢子萌发液中具有能够帮助稻瘟病菌侵染的物质。利用SDSPAGE电泳分析稻瘟病菌孢子萌发液，发现孢子萌发液中含有蛋白质类物质。进一步利用生物质谱鉴定和数据库资源检索，共检索到8个稻瘟病菌的蛋白质基因，其基因编号分别为MGG_01305，MGG_13188，MGG_12454等。提示这些蛋白质可能在稻瘟病菌侵染过程中具有重要的功能。

关键词: 稻瘟病菌, 分泌蛋白, 孢子萌发, 质谱

CLC Number:

LIU Yong-Feng, CHEN Zhi-Yi, YU Wen-Yuan, YU Jun-Jie, HU Jian-Kun, LIU You-Zhou, NIE Ya-Feng. Primary Study on Effect of Secreted Proteins of Magnaporthe oryzae During Infection[J]. Chinese Journal of Rice Science, 2011, 25(4): 452-454.

刘永锋, 陈志谊, 俞文渊, 于俊杰, 胡建坤, 刘邮洲, 聂亚锋. 稻瘟病菌分泌蛋白质在致病过程中的作用初探[J]. 中国水稻科学, 2011, 25(4): 452-454.

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Primary Study on Effect of Secreted Proteins of Magnaporthe oryzae During Infection

稻瘟病菌分泌蛋白质在致病过程中的作用初探

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