Research Progress on Biological Functions of Ubiquitin-conjugating Enzymes in Rice

doi:10.16819/j.1001-7216.2025.250316

Abstract

Abstract:

Ubiquitination is a critical post-translational modification, accounting for 80%-85% of protein degradation in eukaryotes. It regulates protein activity, localization, and function, thereby playing extensive roles in modulating diverse biological processes. The ubiquitin-conjugating enzyme E2, the second key enzyme in the ubiquitination cascade, acts not only as a ubiquitin carrier but also determines the type of ubiquitin linkages and the length of ubiquitin chains. A total of 48 E2 genes have been identified in rice, all encoding proteins that contain a highly conserved UBC (ubiquitin-conjugating) domain. This domain binds to ubiquitin via a thioester bond and forms a specific interaction interface with ubiquitin ligase E3, ensuring the precision of ubiquitin transfer. Studies have shown that E2 enzymes are widely involved in regulating rice growth and development, as well as responses to biotic stresses such as pathogen infection and abiotic stresses including drought and cold.

Key words: ubiquitin-conjugating enzyme, ubiquitination, protein modification, rice

摘要：

泛素化是一种重要的蛋白质翻译后修饰，承担了真核生物中蛋白质降解任务的80%~85%，并影响蛋白的活性、定位和功能，从而广泛参与各种生命活动的调控。泛素结合酶E2是泛素化级联反应中的第二个酶，不仅作为泛素载体，还决定着泛素链的连接方式和长度。水稻中预测存在48个E2基因，所编码蛋白均含有一个高度保守的泛素结合(UBC)结构域。该结构域通过硫酯键与泛素分子结合，同时与泛素连接酶E3形成特异性互作界面，确保泛素转移的精确性。研究表明E2广泛参与调控水稻生长发育与生物/非生物胁迫应答。

关键词: 泛素结合酶, 泛素化, 蛋白修饰, 水稻

WANG Juan, WU Lijuan, HONG Haibo, YAO Zhiwen, WANG Lei, E Zhiguo. Research Progress on Biological Functions of Ubiquitin-conjugating Enzymes in Rice[J]. Chinese Journal OF Rice Science, 2025, 39(6): 744-750.

王娟, 吴丽娟, 洪海波, 姚志文, 王磊, 鄂志国. 水稻泛素结合酶E2的生物学功能研究进展[J]. 中国水稻科学, 2025, 39(6): 744-750.

Figures/Tables 2

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基因名称 Gene name	别名 Gene alias	基因号 Gene ID	染色体 Chromosome	生物学功能 Biological function	参考文献 Reference
OsUBC1	OsSCE1	Os10g0536000	10	影响粒重和结实率，负调控耐旱性 Negatively regulates grain weight, seed-setting rate, and drought tolerance	[35]
OsUBC2	OsSCE1a; OsSCE2	Os03g0123100	3	负调控氮素利用率和光合效率，延长抽穗期 Negatively regulates nitrogen use efficiency and photosynthetic efficiency, and delays heading date	[38]
OsUBC3	OsSCE3	Os04g0580400	4	正调控耐旱性 Positively regulates drought tolerance	[35]
OsUBC7	qMT7-3	Os07g0166800	7	正调控籼稻幼苗的耐寒性 Positively regulates cold tolerance in indica rice seedlings	[32]
OsUBC9	OsRad6	Os03g0791800	3	与SCF泛素连接酶亚基OsSGT1互作 Interacts with the SCF ubiquitin ligase subunit OsSGT1	[21]
OsUBC11		Os01g0839700	1	负调控根系、节间和穗发育 Negatively regulates root, internode, and panicle development	[13]
OsUBC12		Os05g0460200	5	增强粳稻的低温下发芽能力 Enhances germination ability under low temperature conditions in japonica rice	[14]
OsUBC13		Os02g0120600	2	与OsPUB9互作使其稳定 Interacts with and stabilizes OsPUB9	[25]
OsUBC14	OsUBC5a	Os01g0658400	1	负调控细胞死亡和免疫反应 Negatively regulates cell death and immune responses	[26]
OsUBC16		Os04g0667800	4	负调控水稻籽粒大小和重量 Negatively regulates grain size and weight in rice	[24]
OsUBC18		Os09g0293400	9	与OsUBR7配对催化组蛋白H2B单泛素化 Pairs with OsUBR7 to catalyze histone H2B monoubiquitination	[16]
OsUBC24		Os07g0577400	7	调节水稻胚发生和类黄酮生物合成 Regulates rice embryogenesis and flavonoid biosynthesis	[23]
OsUBC26		Os12g0636800	12	正调控稻瘟病抗性 Positively regulates resistance to rice blast	[31]
OsUBC30	OsUEV1B	Os12g0605400	12	调节磷酸盐稳态 Regulates phosphate homeostasis	[17]
OsUBC35	LTN1; OsPHO2	Os05g0557700	5	调节磷酸盐稳态 Regulates phosphate homeostasis	[9,18-20]
OsUBC45	SMG3	Os03g0308000	3	调控穗和籽粒发育 Regulates panicle and grain development	[15]
OsUBC47	OsUBC13	Os01g0673600	1	负调控稻瘟病和白叶枯病抗性 Negatively regulates resistance to rice blast and bacterial blight	[28]

基因名称 Gene name	别名 Gene alias	基因号 Gene ID	染色体 Chromosome	生物学功能 Biological function	参考文献 Reference
OsUBC1	OsSCE1	Os10g0536000	10	影响粒重和结实率，负调控耐旱性 Negatively regulates grain weight, seed-setting rate, and drought tolerance	[35]
OsUBC2	OsSCE1a; OsSCE2	Os03g0123100	3	负调控氮素利用率和光合效率，延长抽穗期 Negatively regulates nitrogen use efficiency and photosynthetic efficiency, and delays heading date	[38]
OsUBC3	OsSCE3	Os04g0580400	4	正调控耐旱性 Positively regulates drought tolerance	[35]
OsUBC7	qMT7-3	Os07g0166800	7	正调控籼稻幼苗的耐寒性 Positively regulates cold tolerance in indica rice seedlings	[32]
OsUBC9	OsRad6	Os03g0791800	3	与SCF泛素连接酶亚基OsSGT1互作 Interacts with the SCF ubiquitin ligase subunit OsSGT1	[21]
OsUBC11		Os01g0839700	1	负调控根系、节间和穗发育 Negatively regulates root, internode, and panicle development	[13]
OsUBC12		Os05g0460200	5	增强粳稻的低温下发芽能力 Enhances germination ability under low temperature conditions in japonica rice	[14]
OsUBC13		Os02g0120600	2	与OsPUB9互作使其稳定 Interacts with and stabilizes OsPUB9	[25]
OsUBC14	OsUBC5a	Os01g0658400	1	负调控细胞死亡和免疫反应 Negatively regulates cell death and immune responses	[26]
OsUBC16		Os04g0667800	4	负调控水稻籽粒大小和重量 Negatively regulates grain size and weight in rice	[24]
OsUBC18		Os09g0293400	9	与OsUBR7配对催化组蛋白H2B单泛素化 Pairs with OsUBR7 to catalyze histone H2B monoubiquitination	[16]
OsUBC24		Os07g0577400	7	调节水稻胚发生和类黄酮生物合成 Regulates rice embryogenesis and flavonoid biosynthesis	[23]
OsUBC26		Os12g0636800	12	正调控稻瘟病抗性 Positively regulates resistance to rice blast	[31]
OsUBC30	OsUEV1B	Os12g0605400	12	调节磷酸盐稳态 Regulates phosphate homeostasis	[17]
OsUBC35	LTN1; OsPHO2	Os05g0557700	5	调节磷酸盐稳态 Regulates phosphate homeostasis	[9,18-20]
OsUBC45	SMG3	Os03g0308000	3	调控穗和籽粒发育 Regulates panicle and grain development	[15]
OsUBC47	OsUBC13	Os01g0673600	1	负调控稻瘟病和白叶枯病抗性 Negatively regulates resistance to rice blast and bacterial blight	[28]