Effect of Low Temperature After Flowering on Grain Quality of indica-japonica Hybrid Rice

doi:10.16819/j.1001-7216.2017.6073

Abstract

Abstract:

【Objective】 The temperature during grain-filling stage of rice is the primary ecologic factor to rice qualities. The research was conducted to reveal the effects of low post-anthesis temperature on rice quality and starch viscosity of Yongyou varieties during grain filling. 【Method】 Four varieties, two indica-japonica hybrid rice (Yongyou538, YY538; Yongyou17,YY17) and two indica rice (Zhongzheyou1, ZZY1) and japonica rice (Zhejing88, ZJ88), were chamber-cultured at temperature as low as 20℃ from 0 to 15 days after flowering(S1), 17℃ from 15 days to 30 days after flowering(S2) and 14 ℃ from 30 days after anthesis to maturity(S3) in a pot experiment. 【Result】 Low temperature after flowering significantly affected the head rice rate, chalkiness, gel consistency, amylose content and protein content of rice, and decreased peak viscosity, hot paste viscosity, cold paste viscosity, breakdown value of Yongyou varieties, and increased setback and consistency. Compared with CK treatment, S1 treatment had the greatest influence on rice processing quality, S2 and S3 treatments had the greatest influence on appearance quality, cooking and eating quality and nutritional quality. Moreover, YY17 is more sensitive to low temperature than YY538. Overall, low temperature after flowering exerted significant impact on grain quality, which varied with genotype, rice quality index and treatment time. 【Conclusion】 The present study indicated that given rice quality indexes differed amony varieties in rice production, we should adopt corresponding measures for high rice quality in grain-filling so as to give the quality potential of indica-japonica hybrid rice into full play.

Key words: indica-japonica hybrid rice, grain-filling stage, low temperature, grain quality, starch viscosity

摘要：

【目的】花后低温是影响米质的首要生态因子。为明确花后低温对甬优品种稻米品质性状及淀粉RVA谱特性的影响,【方法】以籼粳杂交稻甬优17号、甬优538和杂交籼稻中浙优1号、常规粳稻浙粳88为材料,采用盆栽试验,通过人工气候箱于水稻花后0–15d（前期）、15–30d（中期）和30d–成熟（后期）分别进行20℃、17℃和14℃的低温处理。【结果】花后低温显著影响供试品种整精米率、垩白度、胶稠度、直链淀粉含量及蛋白质,使甬优品种淀粉RVA谱特征值峰值黏度、热浆黏度、冷胶黏度、崩解值降低,回复值和消减值升高。花后前期低温对稻米加工品质影响最大,而花后中、后期低温对外观品质、蒸煮食味品质和营养品质影响最大,且甬优17号比甬优538对低温更敏感。花后低温对稻米品质影响明显,低温处理影响因品种类型、品质指标及处理时段而异。【结论】生产中应基于品种特性综合考虑稻米各品质指标,在结实期采取相应的米质调优栽培措施,充分发挥籼粳杂交稻品种的品质潜力。

关键词: 籼粳杂交稻, 灌浆结实期, 不同低温, 稻米品质, 淀粉黏滞性谱

Yanhua ZENG, Yuping ZHANG, Xiaohua PAN, Defeng ZHU, Jing XIANG, Huizhe CHEN, Yikai ZHANG, Yongjun ZENG. Effect of Low Temperature After Flowering on Grain Quality of indica-japonica Hybrid Rice[J]. Chinese Journal OF Rice Science, 2017, 31(2): 166-174.

曾研华, 张玉屏, 潘晓华, 朱德峰, 向镜, 陈惠哲, 张义凯, 曾勇军. 花后不同时段低温对籼粳杂交稻稻米品质性状的影响[J]. 中国水稻科学, 2017, 31(2): 166-174.

Figures/Tables 6

References 30

[1]	Huang M, Jiang L G, Zou Y B, Zhang W X.On-farm assessment of effect of low temperature at seedling stage onearly-season rice quality.Field Crops Res, 2013, 141: 63-68.
[2]	邓飞, 王丽, 叶德成, 任万军, 杨文钰. 生态条件及栽培方式对稻米RVA谱特性及蛋白质含量的影响. 作物学报, 2012, 38(4): 717-724.
	Deng F, Wang L, Ye D C, Ren W J, Yang W Y.Effects of ecological conditions and cultivation methods on rice starch RVA profile characteristics and protein content. Acta Agron Sin, 2012, 38(4): 717-724. (in Chinese with English abstract)
[3]	Asaoka M.Effect of environment temperature at the milky stage on amylose content and fine structure of amylopentin of waxy and nonwaxy endosperm starches of rice.Agric Biol Chem, 1985, 49: 373-378.
[4]	Krishnan P, Swain D K, Bhaskar B C, Nayak S K, Dash R N.Impact of elevated CO2 and temperature on rice yield and methods of adaptation as evaluated by crop simulation studies. Agr Ecosyst Environ, 2007, 122: 233-242.
[5]	王连敏, 王立志, 李忠杰, 王春艳, 刘功, 杨新春. 灌浆阶段低温对寒地水稻碾米及外观品质的影响. 黑龙江农业科学, 2005, (6): 1-4.
	Wang L M, Wang L Z, Li Z J, Wang C Y, Liu G, Yang X C.Effect of low temperature during grain filling stage on milling and appearance quality of rice in cold region.Heilongjiang Agric Sci, 2005, (6): 1-4. (in Chinese with English abstract)
[6]	林洪鑫, 胡启锋, 肖宇龙, 余传源, 何虎, 黄国娟. 寒露风对双季晚稻品种产量构成和品质的影响. 江西农业学报, 2016, 28(5): 20-23.
	Lin H X, Hu Q F, Xiao Y L, Yu C Y, He H, Huang G J.Effect of cold dew wind on quality, yield and yield components of double cropping late rice varieties.Acta Agric Jiangxi, 2016, 28(5): 20-23. (in Chinese with English abstract)
[7]	Chen C, Huang J L, Zhu L Y, Shah F, Nie L X, Cui K H, Peng S B.Varietal difference in the response of rice chalkiness to temperature during ripening phase across different sowing dates. Field Crops Res, 2013, 151: 85-91.
[8]	包劲松. 应用RVA测定米粉淀粉成糊温度. 中国水稻科学, 2007, 21(5): 543-546.
	Bao J S.Accurate measurement of pasting temperature of rice flour by a rapid visco-analyser. Chin J Rice Sci, 2007, 21(5): 543-546. (in Chinese with English abstract)
[9]	贾良, 丁雪云, 王平荣, 邓晓建. 稻米淀粉RVA谱特征及其与理化品质性状相关性的研究. 作物学报, 2008, 34(5): 790-794.
	Jia L, Ding X Y, Wang P R, Deng X J.Rice RVA profile characteristics and correlation with the physical/chemical quality. Acta Agron Sin, 2008, 34(5): 790-794 (in Chinese with English abstract)
[10]	王士强, 宋晓慧, 赵海红, 孙明明, 萧长亮, 顾春梅, 那永光, 解保胜, 曹立勇, 程式华. 孕穗期低温胁迫对寒地水稻产量和品质的影响. 农业现代化研究, 2016, 37(3): 579-586.
	Wang S Q, Song X H, Zhao H H, Sun M M, Xiao C L, Gu C M, Na Y G, Xie B S, Cao L Y, Cheng S H.Effect of cold stress at booting stage on rice yield and quality in the cold region.Res Agric Moder, 2016, 37(3): 579-586 (in Chinese with English abstract)
[11]	朱振华, 金基永, 袁平荣, 赵国珍, 苏振喜, 世荣, 邹茜, 杨世准, 戴陆园. 不同海拔条件下耐冷性粳稻品种的稻米淀粉RVA谱特性. 中国水稻科学, 2010, 24(2):151-156.
	Zhu Z H, Jin J Y, Yuan P R, Zhao G Z, Su Z X, Shi R, Zou Q, Yang S J, Dai L Y.RVA profile properties for cold tolerant and sensitive cultivars of japonica rice at different altitudes in highland region.Chin J Rice Sci, 2010, 24(2): 151-156. (in Chinese with English abstract)
[12]	李刚, 邓其明, 李双成, 王世全, 李平. 稻米淀粉RVA 谱特征与品质性状的相关性. 中国水稻科学, 2009, 23(1): 99-102.
	Li G, Deng Q M, Li S C, Wang S Q, Li P.Correlation analysis between RVA profile characteristics and quality in rice.Chin J Rice Sci, 2009, 23(1): 99-102.(in Chinese with English abstract)
[13]	Ahmed N, Maekawa M, Tetlow I J.Effects of low temperature on grain filling, amylose content, and activity of starch biosynthesis enzymes in endosperm of basmati rice.Aust J Agr Res, 2008, 59: 599-604.
[14]	Mariani L, Parisi S G, Cola G.Space and time behavior of climatic hazard of low temperature for single rice crop in the mid latitude.Int J Clim, 2009, 29: 1862-1871
[15]	花劲, 周年兵, 张军, 张洪程, 霍中洋, 周培建, 程飞虎, 李国业, 黄大山, 陈忠平, 陈国梁, 戴其根, 许轲, 魏海燕, 高辉, 郭保卫. 双季晚稻甬优系列籼粳杂交稻超高产结构与群体形成特征. 中国农业科学, 2015, 48(5): 1023-1034.
	Hua J, Zhou N B, Zhang J, Zhang H C, Huo Z Y, Zhou P J, Cheng F H, Li G Y, Huang D S, Chen Z P, Chen G L, Dai Q G, Xu K, Wei H Y, Gao H, Guo B W.The structure and formation characteristics of super-high yield population with late yongyou series of indica-japonica hybrid rice in double-cropping rice area. Sci Agric Sin, 2015, 48(5): 1023-1034. (in Chinese with English abstract)
[16]	姜元华, 许俊伟, 赵可, 韦还和, 孙建军, 张洪程, 戴其根, 霍中洋, 许轲, 魏海燕, 郭保卫. 甬优系列籼粳杂交稻根系形态与生理特征. 作物学报, 2015, 41(1): 89-99.
	Jiang Y H, Xu J W, Zhao K, Wei H H, Sun J J, Zhang H C, Dai Q G, Huo Z Y, Xu K, Wei H Y, Guo B W.Root system morphological and physiological characteristics ofIndica-japonica hybrid rice of yongyou series. Acta Agron Sin, 2015, 41(1): 89-99. (in Chinese with English abstract)
[17]	曾研华, 张玉屏, 王亚梁, 向镜, 陈惠哲, 朱德峰. 甬优系列杂交稻组合开花期耐冷性评价. 中国水稻科学, 2015, 29(3): 291-298.
	Zeng Y H, Zhang Y P, Wang Y L, Xiang J, Chen H Z, Zhu D F.Evaluation of chilling resistance in Yongyou series of hybrid rice at flowering stage. Chin J Rice Sci, 2015, 29(3): 291-298 (in Chinese with English abstract)
[18]	American Association of Cereal Chemistry (AACC). Methods 61-02 for RVA. Approved Methods of the AACC. 9th ed. St. Paul, M N: The AACC Association, 1995
[19]	吴殿星, 舒庆尧, 夏英武. 利用RVA谱快速鉴别不同表观直链淀粉含量早籼稻的淀粉粘滞特性. 中国水稻科学, 2001, 15(1): 57-59.
	Wu D X, Shu Q Y, Xia Y W.Rapid identification of starch viscosity property of early indica rice varieties with different apparent amylose content by RVA profile. Chin J Rice Sci, 2001, 15(1): 57-59. (in Chinese with English abstract)
[20]	中华人民共和国农业部. 米质测定方法: NY/147–1988. 北京:中国标准出版社,1988.
	Ministry of Agriculture, People's Republic of China. The Standard for the Method of the Rice Quality Determination: NY/147–1988.Beijing: China Standards Press,1988. (in Chinese)
[21]	张荣萍. 灌浆前期低温胁迫对籼粳稻产量和品质的影响. 江苏农业科学, 2015, 43(8): 63-68
	Zhang R P.Effect of low temperature during early grain filling stage on grain yield and quality in indica and japonica rice.Jiangsu Agric Sci, 2015, 43(8): 63-68 (in Chinese with English abstract)
[22]	Lanning S B, Siebenmorgen T J, Counce P A, Ambardekar A, Mauromoustakos A.Extreme nighttime air temperatures in 2010 impact rice chalkiness and milling quality.Field Crops Res, 2011, 124: 132-136
[23]	Ambardekar A A, Siebenmorgen T J, Counce P A, Lanning S B, Mauromoustakos A.Impact of field-scale nighttime air temperatures during kernel development on rice milling quality.Field Crops Res, 2011, 122: 179-185
[24]	Bao J S, Shen S Q, Xie J K.A study on the improvement of eating quality of early indica rice with the assistance of RVA.Mol Plant Breeding, 2004, 2(1):49-53.
[25]	赵国珍, Yang Sea-jun, Yea Jong-doo, 袁平荣, 朱振华, 苏振喜, 戴陆园. 冷水胁迫对云南粳稻育成品种农艺性状的影响. 云南农业大学学报, 2010, 25(2): 158-165.
	Zhao G Z, Yang S J, Yea J D, Yuan P R, Zhu Z H, Su Z X, Dai L Y.Effect of cold water irrigation on agronomic traits of improved japonica rice cultivars from Yunnan Province,China.J Yunnan Agric Univ, 2010, 25(2): 158-165. (in Chinese with English abstract)
[26]	Yuji M, Koji O, Michikazu H.Differences in amylase content, amylographic characteristics and storage proteins of grain on primary and secondary rachis branches in rice.Jpn J Crop Sci, 1995, 64(3): 601-606.
[27]	Lim S J.Varietal variation of amylogram properties and its relationship with other eating quality characteristics in rice.Jpn J Crop Sci, 1995, 27(3): 268-275.
[28]	蔡一霞, 朱庆森, 徐伟, 王维, 杨建昌, 张祖建, 郎有忠. 结实期水分胁迫对水稻强、弱势粒主要米质性状及淀粉粘滞谱特征的影响. 作物学报, 2004,30(3): 241-247.
	Cai Y X, Zhu Q S, Xu W, Wang W, Yang J C, Zhang Z J, Lang Y Z.Effects of water stress on the main characters of superior and inferior grains quality and the properties of RVA profile during grain-filling stage.Acta Agron Sin, 2004, 30(3): 241-247. (in Chinese with English abstract)
[29]	龚金龙, 邢志鹏, 胡雅杰, 张洪程, 戴其根, 霍中洋, 许轲, 魏海燕, 高辉. 籼、粳超级稻主要品质性状和淀粉RVA谱特征的差异研究. 核农学报, 2015, 29(7): 1374-1385.
	Gong J L, Xing Z P, Hu Y J, Zhang H C, Dai Q G, Huo Z Y, Xu K, Wei H Y, Gao H.Difference of main quality traits and RVA profile characteristics of starch between indica and japonica super rice.J Nuclear Agric Sci, 2015, 29(7): 1374-1385. (in Chinese with English abstract)
[30]	袁莉民, 常二华, 徐伟, 王志琴, 杨建昌. 结实期低温对杂交水稻胚乳结构的影响. 作物学报, 2006, 32(1): 96-102.
	Yuan L M, Chang E H, Xu W, Wang Z Q, Yang J C.Effects of low temperature during grain filling on the structure of endosperm in hybrid rice. Acta Agron Sin, 2006, 32(1): 96-102. (in Chinese with English abstract)

处理 Treatment	花后0–15 d 15 DAF	花后15–30 d From 15 to 30 DAF	花后30 d–成熟 30 DAF to maturity
灌浆前期（S₁） Early grain filling stage	20.0	25.0	25.0
灌浆中期（S₂） Middle grain filling stage	25.0	17.0	25.0
灌浆后期（S₃） Later grain filling stage	25.0	25.0	14.0
对照（CK） Controll	25.0

处理 Treatment	花后0–15 d 15 DAF	花后15–30 d From 15 to 30 DAF	花后30 d–成熟 30 DAF to maturity
灌浆前期（S₁） Early grain filling stage	20.0	25.0	25.0
灌浆中期（S₂） Middle grain filling stage	25.0	17.0	25.0
灌浆后期（S₃） Later grain filling stage	25.0	25.0	14.0
对照（CK） Controll	25.0

品种Variety	处理Treatment	加工品质Processing quality			外观品质Appearance quality
品种Variety	处理Treatment	糙米率 BRP / %	精米率 MRP / %	整精米率 HRR / %	垩白粒率 PCRG / %	垩白大小 CS / %	垩白度 DC / %
中浙优1号 Zhongzheyou 1	CK	83.7 a	76.2 a	61.8 a	25.0 b	19.6 a	4.9 a
	S₁	82.8 ab	75.1 ab	41.9 b	29.0 a	10.3 c	3.0 c
	S₂	83.2 ab	75.7 a	46.1 b	23.5 b	17.0 b	4.0 b
	S₃	82.3 b	74.9 b	42.1 b	24.0 b	15.0 bc	3.6 bc
	平均值Average	83.0	75.5	48.0	25.4	15.5	3.9
	CV	0.72	0.77	19.63	9.84	25.26	20.58
甬优17号 Yongyou 17	CK	82.8 a	75.1 b	64.4 a	15.0 b	13.3 b	2.0 b
	S₁	81.6 b	73.9 b	47.8 c	7.5 c	10.7 c	0.8 c
	S₂	82.1 ab	74.5 b	50.3 bc	20.0 a	17.5 a	3.5 a
	S₃	81.8 ab	80.0 a	58.9 ab	12.0 b	16.7 a	2.0 b
	平均值Average	82.1	75.9	55.4	13.6	14.5	2.1
	CV	0.66	3.70	13.85	38.53	21.65	53.29
甬优538 Yongyou 538	CK	85.0 a	77.3 a	62.1 ab	43.5 a	21.6 a	9.4 a
	S₁	84.9 a	77.0 a	61.1 b	32.0 b	20.0 a	6.4 b
	S₂	84.9 a	77.2 a	69.7 a	32.5 b	16.0 b	5.2 c
	S₃	83.8 b	75.9 b	58.9 b	32.0 b	20.0 a	6.4 b
	平均值Average	84.6	76.9	63.0	35.0	19.4	6.9
	CV	0.69	0.82	7.44	16.20	12.33	26.16
浙粳88 Zhejing 88	CK	85.6 b	76.4 b	65.5 a	73.5 a	18.8 a	13.8 a
	S₁	86.4 a	78.4 a	66.0 a	69.5 a	15.0 a	10.4 b
	S₂	86.3 a	78.3 a	50.1 b	64.5 b	16.1 a	10.4 b
	S₃	85.3 b	77.5 a	53.9 b	54.5c	11.7 b	6.4c
	平均值Average	85.9	77.6	58.9	65.5	15.4	10.3
	CV	0.67	1.19	13.78	12.53	18.92	29.52

品种Variety	处理Treatment	加工品质Processing quality			外观品质Appearance quality
品种Variety	处理Treatment	糙米率 BRP / %	精米率 MRP / %	整精米率 HRR / %	垩白粒率 PCRG / %	垩白大小 CS / %	垩白度 DC / %
中浙优1号 Zhongzheyou 1	CK	83.7 a	76.2 a	61.8 a	25.0 b	19.6 a	4.9 a
	S₁	82.8 ab	75.1 ab	41.9 b	29.0 a	10.3 c	3.0 c
	S₂	83.2 ab	75.7 a	46.1 b	23.5 b	17.0 b	4.0 b
	S₃	82.3 b	74.9 b	42.1 b	24.0 b	15.0 bc	3.6 bc
	平均值Average	83.0	75.5	48.0	25.4	15.5	3.9
	CV	0.72	0.77	19.63	9.84	25.26	20.58
甬优17号 Yongyou 17	CK	82.8 a	75.1 b	64.4 a	15.0 b	13.3 b	2.0 b
	S₁	81.6 b	73.9 b	47.8 c	7.5 c	10.7 c	0.8 c
	S₂	82.1 ab	74.5 b	50.3 bc	20.0 a	17.5 a	3.5 a
	S₃	81.8 ab	80.0 a	58.9 ab	12.0 b	16.7 a	2.0 b
	平均值Average	82.1	75.9	55.4	13.6	14.5	2.1
	CV	0.66	3.70	13.85	38.53	21.65	53.29
甬优538 Yongyou 538	CK	85.0 a	77.3 a	62.1 ab	43.5 a	21.6 a	9.4 a
	S₁	84.9 a	77.0 a	61.1 b	32.0 b	20.0 a	6.4 b
	S₂	84.9 a	77.2 a	69.7 a	32.5 b	16.0 b	5.2 c
	S₃	83.8 b	75.9 b	58.9 b	32.0 b	20.0 a	6.4 b
	平均值Average	84.6	76.9	63.0	35.0	19.4	6.9
	CV	0.69	0.82	7.44	16.20	12.33	26.16
浙粳88 Zhejing 88	CK	85.6 b	76.4 b	65.5 a	73.5 a	18.8 a	13.8 a
	S₁	86.4 a	78.4 a	66.0 a	69.5 a	15.0 a	10.4 b
	S₂	86.3 a	78.3 a	50.1 b	64.5 b	16.1 a	10.4 b
	S₃	85.3 b	77.5 a	53.9 b	54.5c	11.7 b	6.4c
	平均值Average	85.9	77.6	58.9	65.5	15.4	10.3
	CV	0.67	1.19	13.78	12.53	18.92	29.52

品种 Variety	处理时段 Treatment period	峰值黏度 PV / RVU	热浆黏度 HPV / RVU	冷胶黏度 CPV / RVU	崩解值 BD / RVU	回复值 SB / RVU	消减值 CT / RVU	糊化温度 PaT / ℃
中浙优1号 ZZY1	对照CK	302.0 a	158.0 a	235.2 b	152.2 a	-66.8 c	85.3 c	81.6 b
	S₁	274.3 b	150.6 a	244.0 ab	123.8 b	-30.3 b	93.4 b	82.5 b
	S₂	230.6 c	138.5 b	247.0 a	92.1 c	16.4 a	108.5 a	86.1 a
	S₃	276.1 ab	144.7 ab	240.0 ab	131.4 b	-36.1 b	95.3 b	82.9 b
	平均Average	270.8	147.9	241.6	124.9	-29.2	95.6	83.3
	CV / %	10.9	5.6	2.1	19.9	117.6	10.0	2.4
甬优17 YY17	对照CK	342.0 a	181.8 a	272.3 a	160.3 a	-69.8 d	90.5 b	80.9 a
	S₁	300.3 b	167.2 b	251.5 c	133.1 b	-48.8 c	84.3 c	80.1 a
	S₂	276.1 c	151.9 c	260.3 b	124.2 c	-15.8 a	108.4 a	80.5 a
	S₃	278.8 c	145.7 c	251.9 c	133.1 b	-26.9 b	106.3 a	78.9 a
	平均Average	299.3	161.6	259.0	137.7	-40.3	97.4	80.1
	CV / %	10.2	10.0	3.8	11.4	59.4	12.1	1.1
甬优538 YY538	对照CK	247.5 a	164.9 a	264.8 a	82.6 a	17.3 d	99.8 b	85.7 a
	S₁	219.9 bc	147.2 bc	258.3 ab	72.8 b	38.4 b	111.2 a	85.4 a
	S₂	204.5 c	140.8 c	249.1 b	63.8 c	44.6 a	108.3 a	87.3 a
	S₃	232.8 b	158.3 ab	258.5 ab	74.6 b	25.7 c	100.3 b	81.7 a
	平均Average	226.2	152.8	257.7	73.4	31.5	104.9	85.0
	CV / %	8.1	7.1	2.5	10.5	39.2	5.5	2.8
浙粳88 ZJ88	对照CK	233.8 a	165.0 ab	257.7 b	68.9 a	23.9 c	92.8 ab	74.5 b
	S₁	237.3 a	171.7 a	269.9 a	65.7 a	32.6 b	98.3 a	84.0 a
	S₂	205.3 b	140.0 c	228.1 d	65.3 a	22.8 c	88.1 b	77.3 b
	S₃	205.9 b	159.9 b	247.4 c	46.0 b	41.5 a	87.5 b	73.8 b
	平均Average	220.6	159.1	250.8	61.5	30.2	91.6	77.4
	CV / %	7.9	8.6	7.1	17.0	28.8	5.4	6.0