Effects of Nitrogen Fertilizer Application on the Formation of White-belly and White-core as Well as Biochemical Composition of japonica Rice Grains

doi:10.16819/j.1001-7216.2016.5039

Abstract

Abstract:

The white-belly and white-core mutants (WBRK and WCRK) from Wuyujing 3 were used to clarify the effects of nitrogen application levels (zero nitrogen application,CK; the ratio of basal N fertilizer to topdressing N fertrlizer for panicle initiation 1:1,N5-5; N fertilizer as basal fertilizer N10-0) on the occurrence of white-belly and white-core grains as well as grain chemical components. The results showed that Nitrogen application significantly increased the percentages of white-belly and white-core grains and the latter was more susceptible to nitrogen application. Averaged across nitrogen treatments, WBRK produced grains with similar chemical composition in comparison with those of control. By contrast, WCRK had higher total starch, amylose and amylopectin contents. Also, nitrogen application improved rice milling quality. Compared to CK, rice grains from WBRK showed significantly elevated contents of storage proteins except for albumin as nitrogen topdressing rate increased, while rice grains from WCRK contained significantly higher levels of protein with no significant changes in protein compositions. Nitrogen topdressing increased the contents of the majority of the 17 amino acids, but decreased histidine and arginine contents especially for WCRK. These findings suggested that nitrogen application exerted a significantly different influence on the occurrence of white-belly and white-core kernels and the accumulation of starch and protein during grain filling, indicating the necessity of various formulated strategies to eliminate white-belly and white-core grains.

Key words: rice (Oryza sativa L.), nitrogen fertilizer, white-belly rice grain, white-core rice grain

摘要：

为探讨不同类型垩白水稻对氮肥的响应差异,以武育粳3号的高腹白突变体(WBRK)和高心白突变体(WCRK)为材料,设置不施氮肥(对照)和两个不同的基肥与穗肥比例处理(N5-5和N10-0)共3个处理,分析施氮对腹白米和心白米的发生及籽粒生化组分的影响。结果表明,施用氮肥显著增加了腹白米率和心白米率,且腹白较心白更易受氮肥影响。施氮对WBRK籽粒总淀粉、直链淀粉和支链淀粉含量无显著影响,显著增加了WCRK籽粒淀粉组分的含量,改善了WBRK和WCRK的碾磨品质。与对照相比,N5-5处理显著增加了WBRK籽粒蛋白质及其组分中球蛋白、醇溶蛋白和谷蛋白含量以及WCRK籽粒蛋白质含量。N10-0处理显著降低了WBRK和WCRK籽粒中的蛋白质和谷蛋白含量。N5-5处理下,WBRK和WCRK籽粒中大多数氨基酸含量有所增加,但组氨酸和精氨酸含量有所降低,WCRK表现得更明显。研究表明,氮素对不同类型垩白水稻品种腹白和心白发生及籽粒贮藏物质积累的影响存在差异,显示出生产中采用不同调优栽培措施的必要性。

关键词: 水稻, 氮肥, 腹白籽粒, 心白籽粒

CLC Number:

Min XI, Zhao-miao LIN, Yan-ling ZHAO, Xin-cheng ZHANG, Xiao-yu YANG, Zheng-hui LIU, Gang-hua LI, Shao-hua WANG, Yan-feng DING. Effects of Nitrogen Fertilizer Application on the Formation of White-belly and White-core as Well as Biochemical Composition of japonica Rice Grains[J]. Chinese Journal OF Rice Science, 2016, 30(2): 193-199.

习敏, 林赵淼, 赵艳岭, 张新城, 杨小雨, 刘正辉, 李刚华, 王绍华, 丁艳锋. 氮肥对粳稻籽粒腹白和心白发生及生化组成的影响[J]. 中国水稻科学, 2016, 30(2): 193-199.

Figures/Tables 7

References 22

[1]	Lanning S B, Siebenmorgen T J, Counce P A, et al.Extreme nighttime air temperatures in 2010 impact rice chalkiness and milling quality.Field Crops Res, 2011, 124: 132-136.
[2]	Cheng F M, Zhong L J, Wang F, et al.Differences in cooking and eating properties between chalky and translucent parts in rice grains.Food Chem, 2005, 90: 39-46.
[3]	Areum C, Jin S, Kee-Jong K,et al.Quality of head and chalky rice and deterioration of eating quality by chalky rice.J Crop Sci Biotechnol, 2009, 12(4): 239-244.
[4]	Singh N,Sodhi N S, Kaur M, et al.Physico-chemical, morphological, thermal, cooking and textural properties of chalky and translucent rice kernels.Food Chem, 2003, 82(3): 433-439.
[5]	Hoshikawa K.Rice grain texture and grading standard//Matsuo T, Hoshikawa K, ed. Science of the rice plant. Volume One, Morphology. Tokyo: Food and Agriculture Policy Research Center, 1993: 383-389.
[6]	金正勋, 秋太权, 孙艳丽, 等. 氮肥对稻米垩白及蒸煮食味品质特性的影响. 植物营养与肥料学报, 2001, 7(1): 31-35.
	Jin Z X, Qiu T Q, Sun Y L, et al.Effects of nitrogen fertilizer on chalkiness ratio and cooking and eating quality properties of rice grain. Plant Nytr Fert Sci, 2001, 7(1): 31-35. (in Chinese with English abstract).
[7]	Xiong F, Wang Z, Gu Y J, et al.Effects of nitrogen application time on caryopsis development and grain quality of rice variety Yangdao 6.Rice Sci, 2008, 15(1): 57-62.
[8]	马群, 顾海永, 张庆, 等. 不同生育期类型粳稻品种垩白性状对施氮水平的响应. 植物营养与肥料学报, 2010, 16(6): 1341-1350.
	Ma Q, Gu H Y, Zhang Q, et al.Response of grain chalky traits to nitrogen application levels for different growth-period japonica cultivars.Plant Nutr Fertil Sci, 2010, 16(6): 1341-1350 (in Chinese with English abstract).
[9]	Juliano B O.A simplified assay for milled-rice amylose.Cereal Sci Today, 1971, 16: 334-340.
[10]	Liu Z H, Cheng F M, Cheng W D, et al.Positional variations in phytic acid and protein content within a panicle of japonica rice.J Cereal Sci, 2005, 41: 297-303.
[11]	Bollag D M, Edelstein S J. Protein Methods.New York:Wiley-Liss, Inc, 1990, 50-56.
[12]	Holme D J, Peck H. Analytical Biochemistry.3 ed. New York, USA:Addison Wesley Longman, 1998: 388-393.
[13]	Cheng F M, Liu Y, Liu Z H, et al.Positional variations in chalky occurrence within a rice panicle and its relation to grain nutritional quality.Aust J Agric Res, 2007, 58: 95-103.
[14]	Tsukaguchi T, Iida Y.Effects of assimilate supply and high temperature during grain-filling period on the occurrence of various types of chalky kernels in rice plants (Oryza sativa L.).Plant Prod Sci, 2008, 11(2): 203-210.
[15]	Taira H.Physicochemical properties and quality of rice grains. //Matsuo T, Kumazawa K, Ishii R, et al. Science of the Rice Plant, Volume Two, Physiology. Tokyo:Food and Agriculture Policy Research Center, 1995.
[16]	Qiao J F, Liu Z H, Deng S Y, et al.Occurrence of perfect and imperfect grains of six japonica rice cultivars as affected by nitrogen fertilization.Plant Soil, 2011, 349: 191-202.
[17]	Xi M, Lin Z M, Zhang X C, et al.Endosperm structure of white-belly and white-core rice grains shown by scanning electron microscopy.Plant Prod Sci, 2014, 17: 285-290.
[18]	Zhou L, Chen L, Jiang L,et al.Fine mapping of the grain chalkiness QTL qPGWC-7 in rice (Oryza sativa L.).Theor Appl Genet, 2009, 118: 581-590.
[19]	Yang Q,Suk-Man K, Jae-Keun S.Genetic analysis and QTL mapping for grain chalkiness characteristics of brown rice (Oryza sativa L.).Gene Genom, 2009, 31(2): 155-164.
[20]	Peng B, Wang L Q, Fan C C, et al.Comparative mapping of chalkiness components in rice using five populations across two environments.BMC Genet, 2014, 15: 49.
[21]	Lawlor D W.Carbon and nitrogen assimilation in relation to yield: Mechanisms are the key to understanding production systems.J Exp Bot, 2002, 53(370): 773-787.
[22]	Buchanan B,Gruissem W J, Russell L.Biochemistry & Molecular Biology of Plants. Rockville, MD, USA:American Society of Plant Physiologists, 2002: 358-361.

突变体 Mutant	垩白米率 Percent of chalky grains/%	垩白大小 Area of chalkiness/%	垩白度 Degree of chalkiness/%	腹白米率 Percent of white-belly rice gains/%	心白米率 Percent of white-core rice gains/%
WBRK	61.78±3.67	26.74±1.56	16.52±0.96	61.78±3.67	0.00±0.00
WCRK	59.67±0.94	80.63±2.76	45.56±1.56	3.17±1.65	56.50±0.71

突变体 Mutant	垩白米率 Percent of chalky grains/%	垩白大小 Area of chalkiness/%	垩白度 Degree of chalkiness/%	腹白米率 Percent of white-belly rice gains/%	心白米率 Percent of white-core rice gains/%
WBRK	61.78±3.67	26.74±1.56	16.52±0.96	61.78±3.67	0.00±0.00
WCRK	59.67±0.94	80.63±2.76	45.56±1.56	3.17±1.65	56.50±0.71

突变体与处理 Mutant and treatment	穗长 Panicle length /cm	单位面积穗数 Number of panicles per 1m²	每穗粒数 Number of spikelets per panicle	结实率 Seed-setting rate /%	千粒重 1000-grain weight /g	产量 Yield /(kg·hm^-2)
WBRK
对照CK	14.46±0.3 c	312.68±17.3 b	89.41±5.3 b	86.18±0.2 c	27.38±0.5 a	6614.38±816.1 b
N5-5	17.16±0.4 a	419.57±47.9 a	111.80±4.0 a	89.23±1.4 b	26.20±0.4 b	10991.99±1053.7 a
N10-0	15.56±0.1 b	440.86±14.3 a	105.54±8.6 a	91.71±2.2 a	26.75±0.5 ab	11383.11±469.9 a
平均Mean	15.73	391.04	102.25	89.04	26.78	9663.16
WCRK
对照CK	13.72±0.2 c	308.10±15.4 b	92.09±6.6 b	78.70±1.4 b	27.38±0.4 a	6102.68±313.8 b
N5-5	16.30±0.3 a	430.21±31.1 a	100.18±3.5 a	86.16±1.0 a	26.82±0.2 b	9966.34±892.6 a
N10-0	15.14±0.1 b	420.82±58.0 a	100.76±2.0 a	85.99±1.3 a	27.09±0.3 ab	9896.93±1144.9 a
平均Mean	15.06	386.38	97.68	83.62	27.10	8655.32

突变体与处理 Mutant and treatment	穗长 Panicle length /cm	单位面积穗数 Number of panicles per 1m²	每穗粒数 Number of spikelets per panicle	结实率 Seed-setting rate /%	千粒重 1000-grain weight /g	产量 Yield /(kg·hm^-2)
WBRK
对照CK	14.46±0.3 c	312.68±17.3 b	89.41±5.3 b	86.18±0.2 c	27.38±0.5 a	6614.38±816.1 b
N5-5	17.16±0.4 a	419.57±47.9 a	111.80±4.0 a	89.23±1.4 b	26.20±0.4 b	10991.99±1053.7 a
N10-0	15.56±0.1 b	440.86±14.3 a	105.54±8.6 a	91.71±2.2 a	26.75±0.5 ab	11383.11±469.9 a
平均Mean	15.73	391.04	102.25	89.04	26.78	9663.16
WCRK
对照CK	13.72±0.2 c	308.10±15.4 b	92.09±6.6 b	78.70±1.4 b	27.38±0.4 a	6102.68±313.8 b
N5-5	16.30±0.3 a	430.21±31.1 a	100.18±3.5 a	86.16±1.0 a	26.82±0.2 b	9966.34±892.6 a
N10-0	15.14±0.1 b	420.82±58.0 a	100.76±2.0 a	85.99±1.3 a	27.09±0.3 ab	9896.93±1144.9 a
平均Mean	15.06	386.38	97.68	83.62	27.10	8655.32

突变体 Mutant	处理 Treatment	腹白米率 Percentage of white-belly rice/%	心白米率 Percentage of white-core rice/%	完善米率 Percentage of perfect rice/%	其他米率 Percentage of other rice/%
WBRK	对照CK	47.29±1.9 b	0.00±0.0 a	51.88±2.2 a	0.83±0.6 b
	N5-5	64.50±2.8 a	0.00±0.0 a	30.21±4.1 b	5.29±2.1 a
	N10-0	63.17±2.8 a	0.00±0.5 a	32.04±2.2 b	4.79±1.2 a
	平均Mean	58.32	0.00	38.04	3.64
WCRK	对照CK	3.50±1.4 c	51.96±3.4 b	43.46±4.0 a	1.08±0.6 a
	N5-5	8.17±3.2 b	58.46±4.0 a	31.79±4.8 b	1.58±1.0 a
	N10-0	11.13±2.7 a	54.38±3.1 b	32.92±3.9 b	1.58±1.4 a
	平均Mean	7.60	54.93	36.06	1.41