中国水稻科学

• 实验技术 • 上一篇    下一篇

稻曲病的接种方法及其效果初探

张君成1; 张炳欣1; 陈志谊2; 刘永锋2; 陆凡2   

  1. 1浙江大学 农业与生物技术学院 植物保护系, 浙江 杭州 310029; 2江苏省农业科学院 植物保护研究所, 江苏 南京 21001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-10-10 发布日期:2003-10-10

Preliminary Study on Inoculation Method of Rice False Smut and Its Effect

ZHANG Jun-cheng 1; ZHANG Bing-xin 1; CHEN Zhi-yi 2; LIU Yong-feng 2; LU Fan 2   

  1. 1 Department of Plant Protection; College of Agriculture and Biotechnology; Zhejiang University; Hangzhou 310029; China; 2 Plant Protection Institute; Jiangsu Academy of Agricultural Sciences; Nangjing 210014; China);
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-10-10 Published:2003-10-10

摘要: 在水稻孕穗期于傍晚用注射法人工接种培养的稻曲病菌,发现病菌菌丝碎段与孢子混合可引起水稻严重发病,最高穗发病率可达100%,最多穗病粒数达106粒,最大病情指数达85.5。不同菌株引发的病情差异很大;病菌在不同品种上引发的病情也差异悬殊;病情指数与穗发病率之间及病情指数与穗平均病粒数之间存在高度相关。初步发现菌株品种之间存在专化互作现象。

关键词: 稻曲病菌, 菌丝, 分生孢子, 致病力, 人工接种

Abstract: Artificial inoculation of Ustilaginoidea virens (Cooke.) Tak. cultured in vitro was made by injecting at nightfall during booting stage of rice . The mixture of hyphae broken and conidia of the pathogen could cause serious disease of false smut. The maximum percentage of infected panicle(PIP) was 100 %, the maximum number of spore balls(NSB) of a panicle was 106, and the maximum disease index(DI) was 85.5. Disease severities caused by different strains, and disease severities of different rice materials caused by the pathogen were different; and there was obvious correlationship between DI and PIP, and between DI and average NSB of a panicle. It seemed that there existed specific interaction between strain and variety.

Key words: Ustilaginoidea virens, hyphae, conidia, pathogenicity, artificial inoculation