中国水稻科学 ›› 2018, Vol. 32 ›› Issue (6): 610-616.DOI: 10.16819/j.1001-7216.2018.8045

• • 上一篇    

水稻穗腐病菌强致病力且高产伏马菌素菌株筛选

孙磊1,2, 王玲1, 刘连盟1, 侯雨萱1, 黎起秦2,*(), 黄世文1,2,*()   

  1. 1中国水稻研究所,杭州 310006
    2广西大学 农学院,南宁 530003
  • 收稿日期:2018-04-16 修回日期:2018-06-25 出版日期:2018-11-27 发布日期:2018-11-10
  • 通讯作者: 黎起秦,黄世文
  • 基金资助:
    国家重点研发计划资助项目(2018YFD020030405,2016YFD0200801,2017YFD0300409);中国农业科学院科技创新工程资助项目(CAAS-ASTIP-2013-CNRRI);国家自然科学基金资助项目(31800133);浙江省自然科学基金资助项目(LQ18C140005);浙江省三农六方项目(CTZB-F160728AWZ-SNY1-4);中国农业科学院协同创新项目(CAAS-XTCX2016012)

Screening for Strains of Rice Spikelet Rot Disease Pathogenic Fungus with High Fumonisin Production and Strong Pathogenicity

Lei SUN1,2, Ling WANG1, Lianmeng LIU1, Yuxuan HOU1, Qiqin LI2,*(), Shiwen HUANG1,2,*()   

  1. 1China National Rice Research Institute, Hangzhou 310006, China
    2College of Agronomy, Guangxi University, Nanning 530003, China
  • Received:2018-04-16 Revised:2018-06-25 Online:2018-11-27 Published:2018-11-10
  • Contact: Qiqin LI, Shiwen HUANG

摘要:

目的 旨在确立稳定高效的水稻穗腐病人工接种技术,鉴定不同菌株致病力。利用已有的伏马菌素检测方法分析测定两种培养基中不同菌株的伏马菌素合成能力,基于上述方法筛选强致病力高产伏马菌素菌株。方法 分别在水稻孕穗期和抽穗期采用注射法和喷雾法接种,观察不同时期、不同接种方法下层出镰刀菌的致病性和稳定性;利用适合的接种方法和液相色谱-串联质谱检测法比较不同菌株的致病力和产毒能力。结果 在花粉母细胞减数分裂期-花粉母细胞成熟期采用注射法接种水稻穗腐病,发病率较高且稳定;在花粉母细胞形成期-始穗期接种对水稻产量影响较大。基于该方法成功筛选出了强致病力菌株FP4、FP6、FP8、FP9、FP10;经HPLC-MS/MS分析测定了层出镰刀菌产生伏马菌素能力,获得了强致病力高产毒菌株FP4和FP9。稻谷培养基比玉米培养基更适合层出镰刀菌合成伏马菌素。结论 水稻穗腐病初侵染为花粉母细胞形成期-花粉母细胞减数分裂期,最佳侵染时期为花粉母细胞形成期-齐穗期;穗腐病对水稻产量的影响与感病时期密切相关。

关键词: 水稻穗腐病, 层出镰刀菌, 人工接种, 伏马菌素, 致病力, 液相色谱-串联质谱法

Abstract:

【Objective】 The objective is to develop a stable and efficient artificial inoculation technique to identify the pathogenicity of different strains for rice spikelet rot disease (RSRD), and to test the ability of F. proliferatum to synthesize fumonisin in two culture media by detecting and analyzing the contents of fumonisin. Based on the methods, strains with high fumonisin production and strong pathogenicity were selected. 【Method】 Injection and spraying inoculation methods were adopted at booting and heading stages. The pathogenicity and stability of different inoculation methods at different growing stage of rice were investigated. The pathogenicity and the ability of fumonisin production of different strains were investigated by using suitable inoculation methods and HPLC-MS/MS analysis. 【Result】 The incidence of RSRD is high and stable with injection inoculation at pollen mother cell meiosis to maturing stages, and rice grain yield was significantly affected by inoculation during pollen mother cell formation to initial heading stage. The high pathogenic strains FP4, FP6, FP8, FP9 and FP10 were selected based on injection inoculation. The fumonisin synthesis assaying was conducted by using HPLC-MS/MS, and the strains FP4 and FP9, with high fumonisin production and strong pathogenicity were identified finally. The culture media made of rice grains was more suitable than that making of corn for fumonisin synthesis.【Conclusion】 The invasion period of F. proliferatum of RSRD was pollen mother cell formation to full heading stage, the initial infection stage was pollen mother cell formation to meiosis stage, and the effect of RSRD on rice yield was closely related to infection stage.

Key words: rice spikelet rot disease, Fusarium proliferatum, artificial inoculation, fumonisin, pathogenicity, HPLC-MS/MS

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