高原粳稻子预44抗稻瘟病基因遗传分析和定位

中国水稻科学

高原粳稻子预44抗稻瘟病基因遗传分析和定位

张锦文^1,2,谭亚玲³,洪汝科³,范静华⁴,罗琼^4,*,曾千春^1,2,*

¹云南农业大学农学与生物技术学院，云南昆明 650201； ²云南省滇型杂交水稻研究中心，云南昆明650201； ³云南农业大学稻作研究所，云南昆明650201；⁴云南农业大学农业生物多样性与病虫害控制教育部重点实验室，云南昆明650201；*通讯联系人, E-mail: <A href=

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-01-10 发布日期:2009-01-10

Genetic Analysis and Gene Mapping of Rice Blast Resistance in japonica Variety Ziyu 44

ZHANG Jinwen ^1,2, TAN Yaling ³, HONG Ruke ³, FAN Jinghua ⁴, LUO Qiong ^4,* , ZENG Qianchun ^1,2,*

¹ Faculty of Agronomy and Biotechnology， Yunnan Agricultural University, Kunming 650201, China； ²Diantype Hybrid Rice Center of Yunnan Province, Kunming 650201, China； ³Rice Research Institute, Yunnan Agricultural University, Kunming 650201, China；⁴ Key Laboratory of Agricultural Biodiversity and Pests Control, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China;*Corresponding authors, E-mail: qiongbf@yahoo.com.cn; zqch1964@yahoo.com.cn

Received:1900-01-01 Revised:1900-01-01 Online:2009-01-10 Published:2009-01-10

摘要/Abstract

摘要： 以高感稻瘟病的低海拔粳稻江南香糯与高抗稻瘟病的高原粳稻子预44作亲本进行杂交，获得F1、F2、BC1F1和以F2单粒传构建的、由276个株系组成的F7重组自交系群体。分别用稻瘟病菌生理小种ZB13和ZE1对两亲本江南香糯和子预44及其杂交后代群体F1、F2和BC1F1进行抗性接种鉴定和遗传分析。结果表明，子预44对稻瘟病菌生理小种ZB13和ZE1的抗性都表现为单基因控制的显性遗传。进一步利用重组自交系F7群体的266个有效株系作为定位群体，将抗稻瘟病菌生理小种ZE1的基因定位在水稻第11染色体上与SSR标记RM206间遗传距离为0 cM的位置，暂定名为Pizy（t）。这些结果为进一步将子预44中的抗性基因用于水稻抗病育种及该抗稻瘟病基因的克隆奠定了重要基础。

关键词: 粳稻, 稻瘟病, 抗性基因, 重组自交系

Abstract: The F1, F2, BC1F1 and 276 F7 recombinant inbred lines (RILs) derived from Ziyu 44(a plateau japonica rice variety from Yunnan Province, China, with durable blast resistance) and Jiangnanxiangnuo (a low latitude japonica rice variety, highly susceptible to blast) were obtained to study the inheritance of rice blast resistance. The F1, F2 and BC1F1 were inoculated with blast races ZB13 and ZE1, respectively. Genetic analyses on the three populations of F1, F2 and BC1F1 showed that the rice blast resistance to the races ZB13 and ZE1 were both controlled by a single dominant gene, respectively. Furthermore, a population including 266 F7 RILs from the 276 F7 RILs was used to map the rice blast resistance gene to the race ZE1. The gene was mapped on rice chromosome 11 with a distance of 0 cM to SSR marker RM206 and tentatively designated as Pizy(t). These results provide essential information for further utilization of the blast resistance gene in Ziyu 44 in rice disease resistance breeding and positional cloning of the gene.

Key words: japonica rice, rice blast, resistance gene, recombinant inbred line, gene mapping

张锦文谭亚玲,洪汝科,范静华,罗琼曾千春. 高原粳稻子预44抗稻瘟病基因遗传分析和定位[J]. 中国水稻科学 2009,23(1): 31-31～35 . .

ZHANG Jinwen ,TAN Yaling ,HONG Ruke ,FAN Jinghua ,LUO Qiong ,ZENG Qianchun . Genetic Analysis and Gene Mapping of Rice Blast Resistance in japonica Variety Ziyu 44[J]. Chinese Journal of Rice Science 2009,23(1): 31-31～35 ..

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