以广东高州普通野生稻为供体亲本的水稻单片段代换系构建

doi:10.3969/j.issn.1001-7216.2010.02.17

摘要/Abstract

摘要： 以栽培稻品种粤香占为受体亲本，利用杂交、回交和微卫星标记辅助选择相结合的方法，构建了以高州普通野生稻为供体亲本的水稻单片段代换系群体。该群体由20个编号的9个单片段代换系构成。这9个单片段代换系分别分布在水稻的第1、2、3、10和11染色体上，代换片段长度为8.1～23.8 cM，总长度为152.7 cM，平均长度为17.0 cM，代换片段对水稻基因组的覆盖总长度为136.1 cM，覆盖率为7.5%。构建以高州普通野生稻为供体亲本的水稻单片段代换系，为发掘和利用高州普通野生稻的有利基因提供了理想的试验材料，为进一步开展高州普通野生稻的遗传研究提供了一条新途径。

关键词: 普通野生稻, 微卫星标记, 分子标记辅助选择, 单片段代换系

Abstract: By using the cultivated rice variety Yuexiangzhan as recipient parent and Gaozhou common wild rice (Oryza rufipogon Griff.) indigenous to Gaozhou, Guangdong Province, China as donor parent, a single segment substitution line (SSSL) population was constructed through hybridization, backcross and microsatellite markerassisted selection. The SSSL population consisted of 20 accessions among which nine SSSLs were different. The substituted segments in the SSSLs distributed on chromosomes 1, 2, 3, 10 and 11, respectively. The estimated lengths of the substituted segments ranged from 81 cM to 23.8 cM, with a total of 152.7 cM and an average of 17.0 cM. The substituted segments covered 136.1 cM, i.e. 75% of rice genome. The construction of SSSLs using Gaozhou common wild rice as donor parent provides novel germplasm for exploiting and utilizing elite genes from Gaozhou common wild rice and puts forward a new path for further genetic studies on Gaozhou common wild rice.

Key words: Oryza rufipogon, microsatellite marker, molecular markerassisted selection, single segment substitution line

赵杏娟,刘向东,李金泉,卢永根. 以广东高州普通野生稻为供体亲本的水稻单片段代换系构建[J]. 中国水稻科学 2010,24(2): 210-214 . , DOI: 10.3969/j.issn.1001-7216.2010.02.17 .

ZHAO Xingjuan,LIU Xiangdong,LI Jinquan*,LU Yonggen* . Construction of Single Segment Substitution Lines in Rice by Using Gaozhou Common Wild Rice(Oryza rufipogon) as Donor Parent[J]. Chinese Journal of Rice Science 2010,24(2): 210-214 ., DOI: 10.3969/j.issn.1001-7216.2010.02.17 .

参考文献

[1]卢宝荣, 宋志平, 戎俊, 等．野生稻遗传资源的可持续和安全利用//中国野生稻研究与利用．第一届全国野生稻大会论文集. 北京：气象出版社, 2003： 26-35.

[2]钟代彬, 罗利军, 应存山．野生稻有利基因转移研究进展．中国水稻科学， 2000， 14（2）： 103-106.

[3]李晨, 孙传清, 穆平, 等．栽培稻与野生稻BC1群体分子连锁图的构建和株高的QTL分析．中国农业大学学报, 2001, 6(5)： 19-24.

[4]杨庆文, 张万霞, 时津霞, 等．广东高州普通野生稻（Oryza rufipogon Griff.）的遗传多样性和居群遗传分化研究．植物遗传资源学报, 2004, 5(4)： 315-319.

[5]李晨, 潘大建, 毛兴学, 等．用SSR标记分析高州野生稻的遗传多样性．科学通报, 2006, 51(5)： 551-558.

[6]Tanksley S D, Grandillo S, Fulton T M, et al. Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L. pimpinellifolium. Theor Appl Genet, 1996, 92: 213-224.

[7]李德军, 孙传清, 付永彩, 等．利用AB-QTL法定位江西东乡野生稻中的高产基因．科学通报, 2002, 47(11)： 854-858.

[8]刘凤霞, 孙传清, 谭禄宾, 等．江西东乡野生稻孕穗开花期耐冷基因定位．科学通报, 2003， 48(17)： 1864-1867.

[9]郝伟, 金健, 孙世勇, 等．覆盖野生稻基因组的染色体片段替换系的构建及其米质相关数量性状基因座位的鉴定．植物生理与分子生物学学报, 2006, 32(3)： 354-362.

[10]Zhou S X, Tian F, Zhu Z F, et al. Identification of quantitative trait loci controlling drought tolerance at seedling stage in Chinese Dongxiang common wild rice (Oryza rufipogon Griff.). Acta Genet Sin, 2006, 33(6): 551-558.

[11]Tian F, Li D J, Fu Q, et al. Construction of introgression lines carrying wild rice (Oryza rufipogon Griff.) segments in cultivated rice (Oryza sativa L.) background and characterization of introgressed segments associated with yield-related traits. Theor Appl Genet, 2006, 112: 570-580.

[12]He G M, Lou X J, Tian F, et al. Haplotype variation in structure and expression of a gene cluster associated with a quantitative trait locus for improved yield in rice. Genome Res, 2006, 16: 618-626.

[13]赵芳明, 刘桂富, 朱海涛, 等．用单片段代换系对不同时期水稻分蘖数QTL的非条件和条件定位．中国农业科学， 2008， 41（2）： 322-330.

[14]刘冠明, 李文涛, 曾瑞珍, 等．水稻亚种间单片段代换系的建立. 中国水稻科学, 2003， 17（3）： 201-204.

[15]何风华, 席章营, 曾瑞珍, 等．利用高代回交和分子标记辅助选择建立水稻单片段代换系. 遗传学报， 2005， 32（8）： 825-831.

[16]曾瑞珍, 张泽民, 何风华, 等．水稻Wx复等位基因的鉴定及单片段代换系的建立．中国水稻科学， 2005, 19(6)： 495-500.

[17]曾瑞珍, 施军琼, 黄朝锋, 等．籼稻背景的单片段代换系群体的构建．作物学报， 2006， 32（1）： 88-95.

[18]Xi Z Y, He F H, Zeng R Z, et al. Development of a wild population of chromosome single-segment substitution lines in the genetic background of an elite cultivar of rice (Oryza sativa L.). Genome, 2006, 49: 476-484.

[19]杨培周, 郭海滨, 赵杏娟, 等．广东高州普通野生稻生殖特性的研究: Ⅰ.结实率、花粉育性及其发育特点．植物遗传资源学报, 2006, 7(1)： 7-12.

[20]杨培周, 郭海滨, 赵杏娟, 等．广东高州普通野生稻生殖特性的研究: Ⅱ.胚囊育性、胚囊发育、胚胎发生和胚乳发育．植物遗传资源学报, 2006, 7(2)： 136-143.

[21]杨培周, 刘向东, 李金泉, 等．高州普通野生稻主要生殖性状的数理统计分析．植物遗传资源学报, 2006, 7(4)： 393-397.

[22]潘大建, 范芝兰, 朱小源, 等．广东高州普通野生稻稻瘟病抗性鉴定．植物遗传资源学报, 2008, 9(3)： 358-361.

[23]练子贤, 史磊刚, 卢永根, 等．广东高州普通野生稻与粳稻杂交F1花粉育性及其发育特点．植物遗传资源学报, 2008, 9(1)： 6-10.

[24]练子贤, 魏常敏, 卢永根, 等．广东高州普通野生稻与粳稻杂交F1胚囊育性及发育特点．中国水稻科学, 2008, 22(3)： 266-272.

[25] 黄朝锋．水稻PSM标记的发展及抗虫基因的分子定位[D]．广州：华南农业大学, 2003.

[26]Zhang G Q, Zeng R Z, Zhang Z M, et al. The construction of a library of single segment substitution lines in rice (Oryza sativa L.)．Rice Genet Newsl, 2004, 21: 85-87.

[27]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism(SSLP) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 252: 597-607.

[28]Li W T, Zeng R Z, Zhang Z M, et al. Mapping of S-b locus for F1 pollen sterility in cultivated rice using PCR based markers. Acta Bot Sin, 2002, 44(4): 463-467.

[29]Young N D, Tanksley S D. Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet, 1989, 77: 95-101.

[30]孙传清, 田丰．超级稻遗传基础与资源创新研究进展//中国超级稻发展战略研讨会论文集. 沈阳： [出版者不详], 2006: 64-73.

[31]Doi K, Iwata N, Yoshimura A. The construction of chromosome substitution lines of African rice (Oryza glaberrima Steud.) in the background of japonica rice (O. sativa L.). Rice Genet Newsl, 1997, 14: 39-41.

[32]Jena K K, Khush G S, Kochert G. RFLP analysis of rice (Oryza sativa L.) introgression lines. Theor Appl Genet, 1992, 84: 608-616.

[33]Sobrizal, Ikeda K, Sanchez P L, et al. Development of Oryza glumaepatula introgression lines in rice, O. sativa L. Rice Genet Newsl, 1999, 16: 107-108.

[34]Kurakazu T, Sobrizal, Ikeda K, et al. Oryza meridionalis chromosomal segment introgression lines in cultivated rice, Oryza sativa L. Rice Genet Newsl, 2001, 18: 81-82.

[35] 李金泉, 杨秀青, 卢永根. 水稻中山1号及其衍生品种选育和推广的回顾与启示. 植物遗传资源学报, 2009, 10(2): 317-323.