中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

宁夏稻瘟病菌的交配型与育性

李文强1，2; 王源超1;郑小波1，*   

  1. 1南京农业大学 植物保护学院/农业部病虫监测与治理重点开放实验室， 江苏 南京 210095； 2宁夏大学 农学院， 宁夏 银川 750021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-11-10 发布日期:2007-11-10

Mating Type and Fertility of Magnaporthe grisea Populations from Rice in Ningxia Hui Autonomous Region,China

LI Wen-qiang , WANG Yuan-chao , ZHENG Xiao-bo   

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-11-10 Published:2007-11-10
  • Contact: ZHENG Xiao-bo

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摘要： 用4个高育性标准菌株对2002-2005年在宁夏9个种植水稻的市县采集的460个稻瘟病菌单孢菌株的交配型和育性进行了测定。在460个菌株中有363个（78.9％）能与4个标准菌株之一杂交产生子囊壳，其余97个（21.1％）菌株不能与4个标准菌株中的任何一个杂交产生子囊壳，是不育菌株。所有363个可育菌株都是交配型MAT11的雄性菌株，没有发现交配型是MAT12的菌株，也没有发现雌性可育菌株和两性可育菌株。在363个可育菌株中，有79个(21.8%)产生子囊壳、子囊和子囊孢子，其余菌株产生的子囊壳中没有子囊孢子。地理位置、年份和水稻品种对宁夏稻瘟病菌群体的交配型分布和育性没有影响。用PCR技术对宁夏的稻瘟病菌的交配型进行了快速检测，所有460个菌株都属于交配型MAT11。这一结果与标准菌株检测的结果一致。只存在一种交配型和缺乏雌性可育菌株表明宁夏稻瘟病菌群体没有有性生殖发生。

关键词: 稻瘟病菌, 有性态, 育性, 交配型

Abstract: To determine mating type and fertility of Magnaporthe grisea populations from rice in Ningxia Hui Autonomous Region,northwestern China,four hundred and sixty isolates from different rice varieties were tested with high fertile standard testers including TH12(MAT1-1),KA3(MAT1-1),TH16(MAT1-2),and Guy11(MAT1-2)in 2002-2005.Of the 460 monoconidial isolates,363(78.9%)were fertile and produced perithecia when paired with either of the four testers,and 97(21.1%)were infertile and did not produce perithecia when mated with any of the four testers.All fertile isolates were male fertile and belonged to a single mating type,MAT1-1,no female fertile and hermaphroditic isolates were detected in sample populations.Among the 363 fertile isolates,79(21.8%)produced perithecia,asci,and ascospores,others produced only barren perithecia.Mating type distribution and fertility status were irrespective of the host variety or geographical location or year of collection of isolates.In a PCR-based mating type assay,the total 460 M.grisea field isolates were identified as MAT1-1,which entirely corroborated the result of standard mating assays using fertile testers.The presence of only one mating type and the absence of female fertile isolates indicated that sexual reproduction was rare or absent in M.grisea populations associated with rice in Ningxia Hui Autonomous Region,northwestern China.

Key words: Magnaporthe grisea, perfect stage, fertility, mating type, rice