Abstract：
A thermo-insensitive pale green leaf mutant(pgl2) was isolated from T-DNA inserted transgenic lines of rice(Oryza sativa L.subsp.japonica cv.Nipponbare).Genetic analysis indicated that the phenotype was controlled by a recessive mutation in a single nuclear-encoded gene.To map the PGL2 gene,an F2 population was constructed by crossing the mutant with Longtefu(Oryza sativa L.subsp.indica).The PGL2 locus was roughly linked to SSR marker RM331 on chromosome 8.Therefore,14 new INDEL markers were developed around the marker.PGL2 gene was further mapped to a 2.37 Mb centromeric region.There was no obvious difference between the mutant and wild type in total chlorophyll content of rice leaves,while the ratio of Chl a/Chl b in the mutant was only about 1,distinctly lower than that in the control,which suggested that the PGL2 gene was related to the transformation between Chl a and Chl b.Moreover,the method of primer design around the centromeric region was discussed,which would provide insight into fine mapping the functional genes in plant centromeres.
ZHU Li ,LIU Wen-zhen ,WU Chao et al. Identification and Fine Mapping of a Gene Related to Pale Green Leaf near Centromere Region in Rice(Oryza sativa L.)[J]. , 2007, 21(3): 228-234 .
2007, Vol. 21 
