中国水稻科学

中国水稻科学

• 实验技术 • 上一篇    下一篇

稻瘟病菌孢子保存技术

孙国昌1; 申宗坦1; 孙漱源2; 陶荣祥2; 施德2   

  1. 1 浙江农业大学; 2 浙江省农业科学院
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1989-04-15 发布日期:1989-04-15

Preservation Technique of Pyricularia oryzae Conidia

Sun Guochang; Shen Zongtan; Sun Shuyuan; Tao Rongxiang; Shi De   

  • Received:1900-01-01 Revised:1900-01-01 Online:1989-04-15 Published:1989-04-15

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摘要： 将载有稻瘟病菌孢子的滤纸置于不同温度和干燥条件下保存,经分期测定,室内干燥保存,经10个月,不论是孢子活力和致病性一般均可维持原有水平。低温干燥保存则能保存更长的时间。这样解决了目前人工接种中稻苗和孢子的培养难以配合的问题。

关键词: 稻瘟病菌, 孢子, 活力, 致病性, 孢子保存

Abstract: Freshly produced conidia were collected from barley grain medium by washing with distilled water, the spore suspension is filtered onto filter paper in a funnal, dried at 30-35℃, and stored in desiccator at indoor, 4℃ and -20℃.The conidia retained their original level of viability and pathogenicity after 10 months indoor,there are further preservation times at 4℃ and -20℃. Thus,the problem is solved that culture rice seedling can not synchronize with sporulation culture of blast fungus during the artifical inoculation.

Key words: Pyricularia oryzae, Conidia, Viability, Pathogenicity, Conidia preservation