The main results are summarized as follows: (1) KT 11.57mg/1 (KT/NAA 1000) is essential for the induction of adventitious buds on the tube-plantlets of autotetraploid rice clones.Maximum induction and propagation of adventitious buds occurred in response to KT- 23.20mg/1 (KT/NAA=2000) level. (2) Different nitrogen and phosphorus levels have strong influence on the propagation of tube-plantlets. The best combination is 10100mg/1 KNO3+ 1320mg/1 (NH4)2SO4(total net N is 120mmol/1 and NO3--N/NH4+-N ratio is 5) and 1190mg/1 KH2PO4. (3) The additional nutrient solution CLN Ⅱ (consisting of 23.20mg/1 KT, 0.01mg/1 NAA, 800mg/1 LH organic supplements and Fe salts of MS)could induce a dramatical number of plantlets when added into culture vessels on the tenth day after transferring.(4) The uptake of sucrose by tube-plantlets was related with KT level.The consuming-rate of sucrose reaches the maximum value (85.5%) under condition of 23.20mg/1 KT+ 0.01mg/1 NAA in our test.