中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

苯并噻二唑诱发水稻对白叶枯病的系统获得抗性

宋凤鸣; 葛秀春; 郑 重; 谢 艳   

  1. 浙江大学 农业与生物技术学院 植物保护系， 浙江 杭州 310029； E-mail:fmsong@zju.edu.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-10-10 发布日期:2001-10-10

Benzothiadiazole-induced Systemic Acquired Resistance in Rice Against Xanthomonas oryzae pv. oryzae

SONG Feng-ming; GE Xiu-chun; ZHENG Zhong; XIE Yan   

  1. (Department of Plant Protection; College of Agriculture and Biotechnology; Zhejiang University; Hangzhou 310029; E-mail: fmsong@zju.edu.cn)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-10-10 Published:2001-10-10

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被引次数

86

摘要： 用苯并噻二唑（benzothiadiazole, BTH)(0.5 mmol/L）、水杨酸（1 mmol/L）、硝酸镍(0.5 mmol/L)、多效唑（300 mg/L）和烯效唑（40 mg/L）处理4叶1心期稻苗后接种白叶枯病菌，病斑长度明显降低。BTH对白叶枯病病菌生长无明显抑制作用。BTH诱发稻苗对白叶枯病抗性的最佳浓度为0.5~1 mmol/L；在诱发处理和接种之间至少需要2 d才能诱导抗性，间隔7 d的诱导抗性效果最好，诱导抗性的持久期至少15 d。BTH处理稻苗第2叶，可使未处理的第3和第4叶上也表现出诱导抗性，但处理后至少需要24~36 h上部叶片才表现出诱导抗性。结果表明，BTH诱导对白叶枯病的抗性是一种系统获得抗性反应。

关键词: 白叶枯病, 系统获得抗性

Abstract: Suppression of the bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae was observed in rice seedlings pretreated with benzothiadiazole (BTH, 0.5 mmol/L), salicylic acid (1 mmol/L), nickelous nitrate (0.5 mmol/L), paclobutrazole (0.3 g/L) or uniconazole (0.04 g/L) 3 days before inoculation with the bacteria, resulting in reduction of lesion length by 14-51%. No significant inhibitory effect of BTH at 1 mmol/L on the growth of X. oryzae pv. oryzae was detected in vitro. The optimum concentration of BTH for resistance induction was found to be at 0.5-1.0 mmol/L. An interval of at least 1 day between BTH induction and challenge inoculation was required for establishment of the induced resistance, among which a 7 day interval gave the highest protection. The BTH induced resistance gave protection from the disease for at least 15 days once induced. Lesion length on the third and the fourth fully expanded leaves was significantly reduced when the lower second leaves were induced by injection with BTH. The development of the BTH induced resistance on the upper leaves required the presence of the lower induced leaves for at least 24 h after treatment with BTH. The results indicate that the BTH induced resistance against the bacterial leaf blight disease was most likely to be a response of systemic acquired resistance.

Key words: Xanthomonas oryzae pv. oryzae, systemic acquired resistance, benzothiadiazole