中国水稻科学

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (2): 251-254.DOI: 10.3969/j.issn.10017216.2012.02.016

• 研究简报 • 上一篇    

快速冷耐受对水稻二化螟滞育幼虫的生理效应

强承魁1,2,# ,于玲雅3,# ,杜予州1,* ,秦越华2 ,赵虎2,胡长效2   

  1. 1扬州大学 应用昆虫研究所， 江苏 扬州 225009；2徐州生物工程职业技术学院 徐州市现代农业生物技术重点实验室， 江苏 徐州 221006；3山东省植物保护总站， 山东 济南 250100；
  • 收稿日期:2011-07-03 修回日期:2011-09-22 出版日期:2012-03-10 发布日期:2012-03-10
  • 通讯作者: 杜予州1,*
  • 基金资助:

    国家973计划资助项目（2012CB114100，2006CB102002）。

Physiological Effects of Rapid Cold Hardening on   Diapause Larvae of Rice Stem Borers, Chilo suppressalis Walker (Lepidoptera: Pyralidae)

QIANG   Chengkui 1,2,# , YU  Lingya 3,# , DU  Yuzhou 1,* , QIN  Yuehua 2 ,  ZHAO  Hu 2 , HU  Changxiao 2   

  1. 1 Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China;  2 Xuzhou Key Laboratory of Modern Agrobiotechnology  Engineering, Xuzhou Bioengineering Technical College, Xuzhou 221006, China;  3 Shandong Station of Plant Protection, Jinan 250100, China;
  • Received:2011-07-03 Revised:2011-09-22 Online:2012-03-10 Published:2012-03-10
  • Contact: DU Yuzhou1,*

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摘要： 为从生理生化水平上探讨快速冷耐受(RCH)对二化螟滞育幼虫的生理效应，分别对正常饲养(CK)、非快速冷耐受(NRCH)和RCH处理过的二化螟幼虫的水分、脂质、总糖、小分子碳水化合物含量及SOD、POD、CAT活性进行了测定。结果表明,RCH处理组水分含量较对照组显著下降，POD活性对照组显著上升，脂质、总糖、海藻糖、葡萄糖、甘油含量及SOD和CAT活性无显著差异；NRCH处理组水分含量较对照组显著降低，而POD活性则显著下降，其余未见显著变化；RCH处理组仅见POD活性较对照组显著增加。由此可见，RCH处理对二化螟滞育幼虫体内水分和POD诱导效应显著。

关键词: 二化螟, 快速冷耐受, 滞育, 生理效应

Abstract:  To understand physiological effects of rapid cold hardening (RCH) on the diapause larvae of the rice stem borer, Chilo suppressalis Walker, at physiological and biochemical levels, the contents of water, lipid, total sugar, lowmolecular weight carbohydrates and the actitives of SOD, POD, CAT in the diapause larvae were measured. The results showed that the content of water in RCHtreated larvae was significantly lower than that  under the check at 0.05 probability level, while the activity of POD was in adverse. The contents of lipid, total sugars, trehalose, glucose, glycerol and the activities of SOD, CAT showed no significant difference. The content of water of  larvae  under nonrapid cold hardening(NRCH) was significantly lower than that  under the check, while the acitivity of POD was in adverse and the rest showed no significant difference. The activities of POD of RCH larvae were just significantly higher than those of NRCHtreated larvae (P<0.05). Therefore,  RCH could enhance physiological effects of water and POD on the diapause larvae.

Key words: Chilo suppressalis Walker, rapid cold hardening, diapause； physiological effect

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