中国水稻科学 ›› 2024, Vol. 38 ›› Issue (6): 709-718.DOI: 10.16819/j.1001-7216.2024.240310
• 研究报告 • 上一篇
姚姝, 陈涛, 赵春芳, 周丽慧, 赵凌, 梁文化, 赫磊, 路凯, 朱镇, 赵庆勇, 管菊, 王才林, 张亚东*()
收稿日期:
2024-03-26
修回日期:
2024-05-09
出版日期:
2024-11-10
发布日期:
2024-11-15
通讯作者:
张亚东
基金资助:
YAO Shu, CHEN Tao, ZHAO Chunfang, ZHOU Lihui, ZHAO Ling, LIANG Wenhua, HAO Lei, LU Kai, ZHU Zhen, ZHAO Qingyong, GUAN Ju, WANG Cailin, ZHANG Yadong*()
Received:
2024-03-26
Revised:
2024-05-09
Online:
2024-11-10
Published:
2024-11-15
Contact:
ZHANG Yadong
摘要:
【目的】研究江淮稻区大面积生产应用和新近育成的优质粳稻外观及食味相关的理化指标特征,以期为该稻区选择适宜品种种植和品种改良提供科学依据。【方法】以近年江淮稻区大面积推广应用的26个粳稻为材料,通过聚类和基因型分析鉴定,分为半糯型粳稻和普通粳稻两大类,比较不同类型粳稻品种外观和食味相关的理化指标,分析稻米各项理化指标与食味品质间的关系。【结果】1)在外观品质方面,两种类型粳稻品种间的垩白粒率、垩白度及透明度差异显著,半糯型粳稻的透明度显著低于普通粳稻。2)在淀粉理化特性方面,半糯型粳稻直链淀粉含量、胶稠度、热浆黏度、冷浆黏度和消减值要显著低于普通粳稻,而崩解值却显著高于普通粳稻。3)在米饭食味值指标方面,半糯型粳稻的米饭外观和普通粳稻无显著差异,但黏度、平衡度、食味值显著高于普通粳稻。4)相关分析表明,稻米外观品质中整精米长宽比,各项理化指标中直链淀粉含量、胶稠度和糊化温度,米饭食味值参数中的外观、硬度与米饭食味显著相关。【结论】两种不同类型粳稻品种外观和蒸煮食味品质差异显著,普通粳稻外观晶莹剔透,而半糯型粳稻外观相对浑浊。普通粳稻具有较高的直链淀粉含量和糊化温度,较低的胶稠度、崩解值和食味值。半糯型粳稻具有较低的直链淀粉含量和糊化温度,较高的胶稠度、崩解值和食味值。
姚姝, 陈涛, 赵春芳, 周丽慧, 赵凌, 梁文化, 赫磊, 路凯, 朱镇, 赵庆勇, 管菊, 王才林, 张亚东. 江淮稻区不同类型粳稻品种外观及蒸煮食味品质特征比较[J]. 中国水稻科学, 2024, 38(6): 709-718.
YAO Shu, CHEN Tao, ZHAO Chunfang, ZHOU Lihui, ZHAO Ling, LIANG Wenhua, HAO Lei, LU Kai, ZHU Zhen, ZHAO Qingyong, GUAN Ju, WANG Cailin, ZHANG Yadong. Analysis on Appearance and Cooking Taste Quality Characteristics of Different Types of japonica Rice in Jianghuai Rice-growing Area[J]. Chinese Journal OF Rice Science, 2024, 38(6): 709-718.
引物序列 Primer sequence | 产物大小 Product size (bp) | 退火温度 Annealing temperature(℃) |
---|---|---|
Wx-F: GGCTGTAAGCACACACAAACTTC | 377 377 | 61 61 |
Wxb -R: GAACACACGGTCGACTCaAC | ||
Wxmp -R: GAACACACGGTCGACTCaAT |
表1 Wx等位基因特异性PCR标记检测引物
Table 1. Wx allele-specific PCR marker detection primers
引物序列 Primer sequence | 产物大小 Product size (bp) | 退火温度 Annealing temperature(℃) |
---|---|---|
Wx-F: GGCTGTAAGCACACACAAACTTC | 377 377 | 61 61 |
Wxb -R: GAACACACGGTCGACTCaAC | ||
Wxmp -R: GAACACACGGTCGACTCaAT |
图1 等位基因特异性PCR标记样品检测结果 A:Wxmp扩增结果;B:Wxb扩增结果。泳道1~13为半糯型粳稻,分别为沪软1212、南粳46、南粳5055、南粳3908、南粳9108、南粳5718、常香粳1813、金香玉1号、宁香粳9号、扬粳5118、扬农香28、徐稻9号和苏香粳3号。泳道14~26为普通粳稻,分别为南粳5758、南粳晴谷、皖垦粳3号、武育粳30、苏垦118、武香粳7368、淮稻5号、武育粳39、苏秀867、泗稻301、徐稻15、连粳18和盐粳15号。
Fig. 1. PCR amplification products by using AS-PCR A, Wxmp amplification product; B, Wxb amplification product. Lanes 1-13 represented semi-waxy japonica rice varieties, i.e. Huruan 1212, Nanjing 46, Nanjing 5055, Nanjing 3908, Nanjing 9108, Nanjing 5718, Changxiangjing 1813, Jinxiangyu 1, Ningxiangjing 9, Yangjing 5118, Yangnongxiang 28, Xudao 9, Suxiangjing 3, respectively. Lanes 14-26 represented common japonica rice varieties, i.e. Nanjing 5758, Nanjingqinggu, Wankenjing 3, Wuyujing 30, Suken 118, Wuxiangjing 7368, Huaidao 5, Wuyujing 39, Suxiu 867, Sidao 301, Xudao 15, Lianjing 18, Yanjing 15, respectively.
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV (%) |
---|---|---|---|---|
整精米粒长 Length of polished rice (mm) | 半糯型粳稻SJ | 4.31~4.95 | 4.62±0.18 a | 3.89 |
普通粳稻CJ | 4.10~5.49 | 4.77±0.36 a | 7.54 | |
整精米粒宽 Width of polished rice (mm) | 半糯型粳稻SJ | 2.24~2.93 | 2.68±0.17 a | 6.43 |
普通粳稻CJ | 2.49~2.75 | 2.63±0.07 a | 2.68 | |
长宽比 Length-width ratio of polished rice | 半糯型粳稻SJ | 1.61~1.92 | 1.73±0.10 b | 5.66 |
普通粳稻CJ | 1.57~2.10 | 1.82±0.14 a | 7.73 | |
垩白率Chalky rice percentage (%) | 半糯型粳稻SJ | 10.85~70.17 | 34.82±20.09 a | 57.70 |
普通粳稻CJ | 4.45~54.80 | 18.01±13.82 b | 76.73 | |
垩白度Chalkiness degree (%) | 半糯型粳稻SJ | 1.80~28.38 | 12.72±8.91 a | 70.02 |
普通粳稻CJ | 1.06~20.16 | 6.41±5.70 b | 89.04 | |
透明度Rice transparency | 半糯型粳稻SJ | 1~4 | 2.54±1.02 a | 40.26 |
普通粳稻CJ | 1~3 | 1.38±0.63 b | 45.72 |
表2 不同类型粳稻品种的粒型、外观品质的差异
Table 2. Differences in grain type and appearance quality of different types of japonica rice varieties
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV (%) |
---|---|---|---|---|
整精米粒长 Length of polished rice (mm) | 半糯型粳稻SJ | 4.31~4.95 | 4.62±0.18 a | 3.89 |
普通粳稻CJ | 4.10~5.49 | 4.77±0.36 a | 7.54 | |
整精米粒宽 Width of polished rice (mm) | 半糯型粳稻SJ | 2.24~2.93 | 2.68±0.17 a | 6.43 |
普通粳稻CJ | 2.49~2.75 | 2.63±0.07 a | 2.68 | |
长宽比 Length-width ratio of polished rice | 半糯型粳稻SJ | 1.61~1.92 | 1.73±0.10 b | 5.66 |
普通粳稻CJ | 1.57~2.10 | 1.82±0.14 a | 7.73 | |
垩白率Chalky rice percentage (%) | 半糯型粳稻SJ | 10.85~70.17 | 34.82±20.09 a | 57.70 |
普通粳稻CJ | 4.45~54.80 | 18.01±13.82 b | 76.73 | |
垩白度Chalkiness degree (%) | 半糯型粳稻SJ | 1.80~28.38 | 12.72±8.91 a | 70.02 |
普通粳稻CJ | 1.06~20.16 | 6.41±5.70 b | 89.04 | |
透明度Rice transparency | 半糯型粳稻SJ | 1~4 | 2.54±1.02 a | 40.26 |
普通粳稻CJ | 1~3 | 1.38±0.63 b | 45.72 |
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准 Mean±SD | 变异系数 CV(%) |
---|---|---|---|---|
蛋白质含量Protein content(%) | 半糯型粳稻SJ | 5.92~7.91 | 6.69±0.48 a | 7.18 |
普通粳稻CJ | 6.07~7.21 | 6.48±0.30 a | 4.62 | |
直链淀粉含量Amylose content(%) | 半糯型粳稻SJ | 7.23~9.96 | 8.54±0.81 b | 9.43 |
普通粳稻CJ | 14.95~17.77 | 15.33±2.05 a | 4.67 | |
胶稠度Gel consistency(mm) | 半糯型粳稻SJ | 71~96 | 85.00±4.94 a | 5.81 |
普通粳稻CJ | 64~81 | 74.21±4.89 b | 6.59 | |
糊化温度Gelatinization temperature(℃) | 半糯型粳稻SJ | 71.23~74.35 | 72.78±0.77 b | 1.06 |
普通粳稻CJ | 71.95~78.20 | 74.67±1.99 a | 2.67 |
表3 不同类型粳稻品种的淀粉理化特征差异
Table 3. Differences in starch physicochemical properties of different types of japonica rice varieties
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准 Mean±SD | 变异系数 CV(%) |
---|---|---|---|---|
蛋白质含量Protein content(%) | 半糯型粳稻SJ | 5.92~7.91 | 6.69±0.48 a | 7.18 |
普通粳稻CJ | 6.07~7.21 | 6.48±0.30 a | 4.62 | |
直链淀粉含量Amylose content(%) | 半糯型粳稻SJ | 7.23~9.96 | 8.54±0.81 b | 9.43 |
普通粳稻CJ | 14.95~17.77 | 15.33±2.05 a | 4.67 | |
胶稠度Gel consistency(mm) | 半糯型粳稻SJ | 71~96 | 85.00±4.94 a | 5.81 |
普通粳稻CJ | 64~81 | 74.21±4.89 b | 6.59 | |
糊化温度Gelatinization temperature(℃) | 半糯型粳稻SJ | 71.23~74.35 | 72.78±0.77 b | 1.06 |
普通粳稻CJ | 71.95~78.20 | 74.67±1.99 a | 2.67 |
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV(%) |
---|---|---|---|---|
峰值黏度Peak viscosity (cP) | 半糯型粳稻SJ | 2555 ~ 3248 | 2837.85±175.63 a | 6.19 |
普通粳稻CJ | 1509 ~ 2826 | 2388.46±313.03 b | 13.11 | |
热浆黏度Trough viscosity (cP) | 半糯型粳稻SJ | 697 ~ 2019 | 1387.77±275.64 b | 19.86 |
普通粳稻CJ | 1023 ~ 2264 | 1739.12±340.26 a | 19.75 | |
最终黏度Final viscosity (cP) | 半糯型粳稻SJ | 1007 ~ 2619 | 1986.35±336.49 b | 16.94 |
普通粳稻CJ | 1313 ~ 3180 | 2488.31±565.37 a | 22.72 | |
崩解值Breakdown (cP) | 半糯型粳稻SJ | 921 ~ 1667 | 1215.62±212.18 a | 17.45 |
普通粳稻CJ | 478 ~ 960 | 792.5±130.79 b | 16.50 | |
消减值Setback (cP) | 半糯型粳稻SJ | −864 ~ −186 | −477.69±188.43 b | 39.45 |
普通粳稻CJ | −1279 ~ −186 | −541.81±265.91 a | 49.08 | |
回复值Consistence (cP) | 半糯型粳稻SJ | 310 ~ 683 | 560.96±81.94 b | 14.61 |
普通粳稻CJ | 140 ~ 1003 | 780.35±255.16 a | 32.70 |
表4 不同类型粳稻品种的RVA特征值差异
Table 4. Differences in starch physicochemical properties of different types of japonica rice varieties
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV(%) |
---|---|---|---|---|
峰值黏度Peak viscosity (cP) | 半糯型粳稻SJ | 2555 ~ 3248 | 2837.85±175.63 a | 6.19 |
普通粳稻CJ | 1509 ~ 2826 | 2388.46±313.03 b | 13.11 | |
热浆黏度Trough viscosity (cP) | 半糯型粳稻SJ | 697 ~ 2019 | 1387.77±275.64 b | 19.86 |
普通粳稻CJ | 1023 ~ 2264 | 1739.12±340.26 a | 19.75 | |
最终黏度Final viscosity (cP) | 半糯型粳稻SJ | 1007 ~ 2619 | 1986.35±336.49 b | 16.94 |
普通粳稻CJ | 1313 ~ 3180 | 2488.31±565.37 a | 22.72 | |
崩解值Breakdown (cP) | 半糯型粳稻SJ | 921 ~ 1667 | 1215.62±212.18 a | 17.45 |
普通粳稻CJ | 478 ~ 960 | 792.5±130.79 b | 16.50 | |
消减值Setback (cP) | 半糯型粳稻SJ | −864 ~ −186 | −477.69±188.43 b | 39.45 |
普通粳稻CJ | −1279 ~ −186 | −541.81±265.91 a | 49.08 | |
回复值Consistence (cP) | 半糯型粳稻SJ | 310 ~ 683 | 560.96±81.94 b | 14.61 |
普通粳稻CJ | 140 ~ 1003 | 780.35±255.16 a | 32.70 |
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV (%) |
---|---|---|---|---|
外观Appearance | 半糯型粳稻SJ | 6.9~9.8 | 8.70±0.75 a | 8.62 |
普通粳稻CJ | 7.4~9.6 | 8.78±0.56 a | 6.42 | |
硬度Hardness | 半糯型粳稻SJ | 3.87~5.53 | 4.61±0.52 b | 11.35 |
普通粳稻CJ | 4.94~6.86 | 5.91±0.52 a | 8.75 | |
黏度Viscosity | 半糯型粳稻SJ | 0.86~1.23 | 1.01±0.10 a | 9.71 |
普通粳稻CJ | 0.62~0.95 | 0.76±0.09 b | 12.12 | |
平衡度Balance degree | 半糯型粳稻SJ | 0.18~0.26 | 0.21±0.02 a | 8.42 |
普通粳稻CJ | 0.12~0.20 | 0.15±0.02 b | 14.08 | |
食味值Taste value | 半糯型粳稻SJ | 76.1~89.4 | 83.59±3.25 a | 3.89 |
普通粳稻CJ | 72.8~82.1 | 78.00±2.49 b | 3.19 |
表5 不同类型粳稻品种的米饭食味参数差异
Table 5. Differences in starch physicochemical characteristics of different types of japonica rice varieties
指标 Index | 品种类型 Type | 变幅 Range | 平均值±标准差 Mean±SD | 变异系数 CV (%) |
---|---|---|---|---|
外观Appearance | 半糯型粳稻SJ | 6.9~9.8 | 8.70±0.75 a | 8.62 |
普通粳稻CJ | 7.4~9.6 | 8.78±0.56 a | 6.42 | |
硬度Hardness | 半糯型粳稻SJ | 3.87~5.53 | 4.61±0.52 b | 11.35 |
普通粳稻CJ | 4.94~6.86 | 5.91±0.52 a | 8.75 | |
黏度Viscosity | 半糯型粳稻SJ | 0.86~1.23 | 1.01±0.10 a | 9.71 |
普通粳稻CJ | 0.62~0.95 | 0.76±0.09 b | 12.12 | |
平衡度Balance degree | 半糯型粳稻SJ | 0.18~0.26 | 0.21±0.02 a | 8.42 |
普通粳稻CJ | 0.12~0.20 | 0.15±0.02 b | 14.08 | |
食味值Taste value | 半糯型粳稻SJ | 76.1~89.4 | 83.59±3.25 a | 3.89 |
普通粳稻CJ | 72.8~82.1 | 78.00±2.49 b | 3.19 |
指标 Index | 相关系数 Correlation coefficient | 指标 Index | 相关系数 Correlation coefficient |
---|---|---|---|
整精米长宽比Length-width ratio of polished rice | −0.366** | 峰值黏度Peak viscosity | −0.132 |
整精米粒长Length of polished rice | −0.208 | 热浆黏度Trough viscosity | −0.049 |
整精米粒宽Width of polished rice | 0.271 | 崩解值Breakdown | 0.190 |
垩白粒率Chalky rice percentage | −0.172 | 最终黏度Final viscosity | −0.072 |
垩白度Chalkiness degree | −0.278* | 消减值Setback | −0.044 |
外观Appearance | 0.797*** | 回复值Consistence | −0.139 |
硬度Hardness | −0.284* | 峰值时间Peak time | 0.118 |
黏度Viscosity | 0.177 | 糊化温度Gelatinization temperature | −0.580*** |
平衡度Balance degree | 0.031 | 直链淀粉含量Amylose content | −0.428** |
蛋白质含量Protein content | −0.045 | 胶稠度Gel consistency | 0.474*** |
表6 各项理化指标与食味值的相关系数
Table 6. Correlation coefficient between physical and chemical indexes and taste value
指标 Index | 相关系数 Correlation coefficient | 指标 Index | 相关系数 Correlation coefficient |
---|---|---|---|
整精米长宽比Length-width ratio of polished rice | −0.366** | 峰值黏度Peak viscosity | −0.132 |
整精米粒长Length of polished rice | −0.208 | 热浆黏度Trough viscosity | −0.049 |
整精米粒宽Width of polished rice | 0.271 | 崩解值Breakdown | 0.190 |
垩白粒率Chalky rice percentage | −0.172 | 最终黏度Final viscosity | −0.072 |
垩白度Chalkiness degree | −0.278* | 消减值Setback | −0.044 |
外观Appearance | 0.797*** | 回复值Consistence | −0.139 |
硬度Hardness | −0.284* | 峰值时间Peak time | 0.118 |
黏度Viscosity | 0.177 | 糊化温度Gelatinization temperature | −0.580*** |
平衡度Balance degree | 0.031 | 直链淀粉含量Amylose content | −0.428** |
蛋白质含量Protein content | −0.045 | 胶稠度Gel consistency | 0.474*** |
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