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10 July 2017, Volume 31 Issue 4 Previous Issue    Next Issue
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Identification of Rice Blast Resistance Mutant lmm326 and Preliminary Analysis of Its Regulatory Pathway Tingting XU, Ning YU, Yingxin ZHANG, Zhenzhen BI, Weixun WU, Yongrun CAO, Beifang WANG, Yue ZHANG, Dandan XUAN, Daibo CHEN, Xiaodeng ZHAN, Shihua CHENG, Liyong CAO 2017, 31(4): 335-344.  DOI: 10.16819/j.1001-7216.2017.7007 335 Abstract ( )   HTML ( )   PDF (21572KB) ( )   【Objective】Rice lesion mimic mutants often have a certain resistance to rice blast. To understand the genetic mechanisms behind its blast resistance, we identified the rice lesion mimic mutant lmm326 and analyzed its regulatory pathway. 【Method】 lmm326 was obtained from an EMS-induced Zhonghua 11 mutant bank, and population derived from lmm326/ Zhonghua 11 and lmm326/Dular were used for genetic and fine mapping. 【Result】At 5-leaf stage, there were brown lesions on lower leaves of lmm326 initially. Compared with the wild type(WT), photosynthetic pigment contents, net photosynthetic rate, plant height, seed setting rate, tiller number, and 1000-grain weight of lmm326 were significantly reduced. Evans blue and DAB staining assay indicated that leaves of lmm326 accumulated more dead cells and H2O2. Additionally, lmm326 displayed higher resistance to 4 races of rice blast compared with WT. According to genetic analysis, the phenotype of mutant was controlled by a single recessive gene. Here, we employed map-based cloning approach to finely map LMM326 through F2 population derived from a cross between lmm326 and Dular, and the gene was narrowed to a 38-kb region on the long arm of chr. 1, which harbored six ORFs. Sequence analysis revealed that a single base substitution at position C433T in Os01g0919900 CDS resulted in a substitution of leucine for phenylalanine at position 145. Real-time PCR showed that expression levels of defense-related genes were significantly higher in lmm326 than in WT.【Conclusion】These results demonstrated that the target gene is allelic to OsSSI2, and LMM326 is likely to participate in salicylic acid-signaling pathway, and its mutation activates the defense response.

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Gene Cloning, Polyclonal Antibody Preparation and Expression Localization of Two dynamin-1-like Genes from Nilaparvata lugens (Hemiptera: Delphacidae) Chenxing ZHAO, Yewei YU, Yipeng XU, Xiaoping YU 2017, 31(4): 345-354.  DOI: 10.16819/j.1001-7216.2017.6165 345 Abstract ( )   HTML ( )   PDF (32997KB) ( )   【Objective】To study the biological function of two dynamin-1-like genes, NlDNM1L-1 and NlDNM1L-2 in Nilaparvata lugens,【Method】 two dynamin-1-like genes were cloned from N. lugens by polymerase chain reaction (PCR), and their bioinformatics analysis was conducted. Using fluorescence quantitative real-time PCR technology, the relative expression levels of NlDNM1L-1 and NlDNM1L-2 in different tissues and at different developmental stages of N. lugens were detected. The expression vectors of the two genes were constructed and recombinant proteins were expressed in Escherichia coli Rosetta. The expressed recombinant proteins were purified by Ni-NTA agarose gel affinity system and then injected into New Zealand white rabbit to generate polyclonal antibodies. The titer of the antibodies was monitored by ELISA, and the immune specificity was determined by Western blotting hybridization. Using above antibodies, the localization of target proteins in ovary was detected by immunofluorescence. 【Result】The two dynamin-1-like genes were cloned, and their GenBank accession numbers were KY082900 and KY082901, respectively. The phylogenetic tree showed that NlDNM1L-1 and NlDNM1L-2 were conserved in Hemipterans. The results of protein structure prediction and space-time expression pattern analysis indicated that NlDNM1L-1 and NlDNM1L-2 might play different roles in N. lugens. The ELISA and Western blotting results showed that the polyclonal antibodies had high titer and good specificity. Immunofluorescence showed that NlDNM1L-1 and NlDNM1L-2 were widely distributed in the follicle cells of N. lugens ovary, indicating that they may be related to the development and maturity of N. lugens ovary. NlDNM1L-1 and NlDNM1L-2 may also be associated with the invasion of the yeast-like symbionts to N. lugens ovary. 【Conclusion】 The DNA sequences, bioinformatics and expression pattern of NlDNM1L-1 and NlDNM1L-2 were clarified. And the polyclonal antibodies of NlDNM1L-1 and NlDNM1L-2 were obtained and their localizations in the ovary have preliminarily been understood. These results lay a foundation for further biological function investigation of NlDNM1L-1 and NlDNM1L-2 in N. lugens.

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Phenotypic Analysis and Gene Mapping of a White Stripe Mutant st13 in Rice Liting SUN, Tianzi LIN, Yunlong WANG, Mei NIU, Tingting HU, Shijia LIU, Yihua WANG, Jianmin WAN 2017, 31(4): 355-363.  DOI: 10.16819/j.1001-7216.2017.6165 355 Abstract ( )   HTML ( )   PDF (16967KB) ( )   【Objective】Rice leaf color mutation identification and cloning of related genes is helpful to research photosynthesis and further understand the mechanism of chloroplast development and pigment synthesis pathway, providing theoretical basis for the breeding of rice with high photosynthetic efficiency.【Method】A somaclonal mutant (designated as stripe leaf 13, st13) was isolated from tissue-cultured progenies of a japonica rice variety Dongjin. The main agronomic traits of wild type and st13 were determined at maturity, and the pigment contents and ultrastructure of chloroplast were observed at the seedling stage. Dongjin and st13 were reciprocally crossed to observe the phenotype of F1 and genetic analysis was carried out. An F2 population and F2:3 population derived from the cross st13 × Nanjing 11 (an indica rice variety) were used for gene mapping of ST13. Quantitative RT-PCR was carried out to analyze the relative expression of genes associated with chloroplast development and chlorophyll biogenesis in the wild-type Dongjin and st13 mutant. 【Result】Compared to the wild type, the plant height, number of effective panicles, panicle length, seed-setting rate, and 1000-grain weight were all significantly reduced in the mutant. In agreement with leaf color, the pigment content of st13 mutant was reduced at the seedling stage but rose to almost the same level as the wild type at the tillering stage. With a transmission electron microscopy, defective chloroplasts with no thylakoid were observed in the st13 mutant. Genetic analysis indicated the mutant phenotype was controlled by a single recessive nuclear gene. ST13 was restricted to an interval between the InDel (insertion/deletion) markers I3-21 and I3-22 on chromosome 3. Six InDel markers were further developed and the ST13 gene was narrowed down to a 94-kb region, containing eight putative open reading frames. 【Conclusion】These eight candidate genes encode three putative proteins and five functional proteins respectively, while none of them have been reported in rice. Thus, ST13 was an unreported gene in rice. Relative to wild type, the expression levels of genes associated with chloroplast development, chlorophyll biosynthesis, and photosynthetic system were significantly altered in the mutant. So we hypothesized that ST13 might be the key gene for regulating chloroplast development.

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Mapping of QTLs for Heading Date Using Whole-genome Re-sequenced Chromosome Segment Substitution Lines in Rice Jun WANG, Jinyan ZHU, Yajun TAO, Yong ZHOU, Fangjun FAN, Wenqi LI, Fangquan WANG, Weigong ZHONG, Jie YANG, Guohua LIANG 2017, 31(4): 364-370.  DOI: 10.16819/j.1001-7216.2017.7017 Abstract ( )   HTML ( )   PDF (10206KB) ( )   【Objective】Heading date is an important agronomic trait in rice, it is a typical quantitative trait controlled by multiple genes. As novel research material, chromosome segment substitution lines are useful in QTL fine mapping and cloning because of minimizing the interference of genetic background among plants. 【Method】In this study, 128 whole-genome re-sequenced chromosome segment substitution lines derived from Nipponbare as donor parent in the background of 9311, were used for mapping QTLs for heading date by combining the sequencing-based Bin-map with multiple linear regression analysis. 【Result】Six QTLs for heading date were identified in two environments and two years. qHD2.1 was mapped in the region of 759 848 bp on the chromosome 2; qHD2.2 was mapped in the region of 45 286 bp on the chromosome 2; qHD 3.1 was mapped in the region of 147 931 bp on the chromosome 3; qHD5.1 was mapped in the region of 213 351 bp on the chromosome 5; qHD5.2 was mapped in the region of 442 305 bp on the chromosome 5; qHD8.1 was mapped in the region of 538 176 bp on the chromosome 8. 【Conclusion】The results are important for the QTLs cloning and provide a foundation for understanding molecular regulation mechanism of heading date in rice.

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Development of Permanent Genic Male Sterile Line by Pyramiding Thermo-sensitive Genic Male Sterile Genes and Reverse Temperature Induced Genic Male Sterile Genes in Rice (Oryza sativa L.) Jianhua JIANG, Jinlong NI, Shuang WU, Dezheng WANG 2017, 31(4): 371-378.  DOI: 10.16819/j.1001-7216.2017.7017 371 Abstract ( )   HTML ( )   PDF (813KB) ( )   ： 【Objective】The security problem of seed production has seriously hampered the steadily and sustainable development of two-line hybrid rice. 【Method】Aizi S (thermos-sensitive genic male sterile, TSGMS) and Aiyan s (reverse temperature sensitive genic male sterile line, RTSGMS) were screened as the original parents to develop permanent genic sterile line (PGSL) according to the F1 fertility of crosses among 16 (light-) TSGMS and 4 RTSGMS. The fertility genes were introduced into the near isogenic lines Tianfeng S and Tianfeng s by backcrossing Aizi S and Aiyan s with the bridge parent Tianfeng B, respectively. Then, Tianfeng Ss, the PGSL, was observed from the cross between Tianfeng S and Tianfeng s. The fertility, restoration, competitive advantage of yield-related traits and reproductive characteristics of Tianfeng Ss were investigated. 【Result】 Tianfeng Ss was completely sterile under natural long day with high temperature and short day with low temperature. And no obvious difference was noted among the F1 crosses from Tianfeng Ss and its three parents and five restorers in spikelet fertility percentage. 【Conclusion】The results showed that the newly developed PGSL might solve the security problem of seed production in two-line hybrid rice fundamentally.

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Effects of Alternate Wetting and Drying Irrigation on Grain Yield in Rice Cultivars with Different Drought Resistance and Its Physiological Mechanism Jinlong BIAN, Yulan JIANG, Yanyang LIU, Yongfang FENG, He LIU, Shiming XIA, Lijun LIU 2017, 31(4): 379-390.  DOI: 10.16819/j.1001-7216.2017.7006 379 Abstract ( )   HTML ( )   PDF (1820KB) ( )   【Objective】We aim to reveal the effects of alternate wetting and irrigation during whole growth period on grain yield in rice cultivars with different drought resistance and its physiological mechanisms.【Method】With four rice cultivars differed in drought resistance, Yangdao 6 and Liangyoupeijiu (indica), Hanyou 8 and Zhendao 88 (japonica), as materials, three water management regimes, i.e. CI (conventional irrigation), WMD (alternate wetting and moderate drying irrigation) and WSD (alternate wetting and severe drying irrigation) during the whole growing season were conducted to investigate their effects on grain yield in a pot experiment.【Result】Compared with CI, WMD significantly increased the grain yields of Yangdao 6 and Hanyou 8 with higher drought resistance by 6.9% and 7.5%, and decreased those of Liangyoupeijiu and Zhendao 88 with lower drought resistance by 7.28% and 8.10%, respectively. Under WSD, the grain yields of four cultivars all decreased significantly, but the yield declines in Liangyoupeijiu and Zhendao 88 were much higher than those in Yangdao 6 and Hanyou 8. Under WMD, root oxidation activity, cytokinin (zeatin + zeatin riboside) content in roots and leaves, leaf photosynthetic rate and water potential, activities of enzymes involved in sucrose-to-starch conversion in grains were all significantly increased in Yangdao 6 and Hanyou 8 after re-watering and they were all nearly the same or decreased in Liangyoupeijiu and Zhendao 88. Under WSD, these indices were all decreased in four rice cultivars. 【Conclusion】 Stronger root oxidation activity, higher cytokinin (zeatin + zeatin riboside) content in roots and leaves, greater photosynthetic rate and water potential of leaves and activities of enzymes involved in sucrose-to-starch conversion in grains after re-watering were the basic physiological characteristics in rice cultivars with strong drought resistance under WMD.

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Effects of Nitrogen Topdressing for Panicle Initiation on Leaf Morphology, Photosynthetic Production and Grain Yield of Two Middle-season Hybrid Rice Jian QIN, Zhiyuan YANG, Yongjian SUN, Hui XU, Tengfei LÜ, Zou DAI, Jiakui ZHENG, Kaifeng JIANG, Jun MA 2017, 31(4): 391-399.  DOI: 10.16819/j.1001-7216.2017.6146 391 Abstract ( )   HTML ( )   PDF (529KB) ( )   【Objective】Nitrogen fertilization has a great influence on rice leaf growth, morphology, photosynthetic production and grain yield, especially the management of spikelet-promoting and spikelet-developing nitrogen. The objectives of this study are to elucidate the effect of spikelet-promoting and spikelet-developing nitrogen application ratios on middle-season hybrid rice.【Method】Two middle-season rice combinations (Dexiang 4103 and Yixiang 3724, which differ greatly in leaf morphology and grain yield) were used. The total nitrogen application rate is 180 kg/hm2, 40% of which is topdressed for panicle initiation. Four kinds of spikelet-promoting and spikelet-developing nitrogen ratios(1∶3, 2∶2, 3∶1, 4∶0) were designed. Leaf morphology (including leaf length, width, angle, leaf area index, and group light transmittance), net photosynthetic rate, dry matter accumulation and grain yield were measured at full heading and harvest stage. 【Result】Lower ratio(1:3) of spikelet-promoting nitrogen fertilizer can achieve a higher grain yield both in the two combinations, and erect leaf morphology, good leaf posture for light, high net photosynthetic rate and large dry matter accumulation were observed simultaneously. On the contrary, relatively low grain yields of both rice combinations were observed under the treatment of high ratio of spikelet-promoting and nitrogen fertilizer. This might be mainly attributed to the much increased leaf area, angle and dropping degree under the higher ratio, which further caused a poor population quality and a decline in seed-setting rate and 1000-grain weight. As for the two different rice combinations, Dexiang 4103 had a higher grain yield. In fact, Dexiang 4103 possessed a larger total number of spikelets, while a smaller leaf area and angle variation for all fresh leaves under different nitrogen application ratios, which allowed Dexiang 4103 to maintain a good population quality and good posture to capture more light. As a result, Dexiang 4103 produced a higher dry matter accumulation and yield.【Conclusion】Based on these results, in order to increase the dry matter accumulation after heading and yield, we suggest that the application of panicle nitrogen fertilizer should shape a good leaf morphology and high quality population. Finally, the leaf breeding of hybrid rice was discussed.

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Characteristics of Population Growth in japonica Rice Varieties with High Nitrogen Use Efficiency and High Yield in Huaibei Area Jian LIANG, Chen ZHAO, Chao HAN, Hongru REN, Mengyun CHEN, Hongcheng ZHANG, Zhongyang HUO 2017, 31(4): 409-416.  DOI: 10.16819/j.1001-7216.2017.7035 Abstract ( )   HTML ( )   PDF (437KB) ( )   【Objective】The aim was to clarify the characteristics of population growth of japonica varieties with high nitrogen use efficiency and high yield in Huaibei Area. 【Method】The yield components, number of stems and tillers,leaf area and population biomass production were investigated under their optimum N levels with 3 representative japonica varieties [high N use efficiency and high yield (HNUEHY), medium N use efficiency and high yield(MNUEHY), high N use efficiency and medium yield(MNUEMY)] as material. 【Result】Number of stems and tillers increased slowly to the peak in jointing stage of HNUEHY, and then decreased gently with the highest percentage of productive tillers. The leaf area indexes of HNUEHY and HNUEMY were the highest and lowest at heading stage. The leaf area index of HNUEHY were 2.53% and 2.58% higher than that of MNUEHY and HNUEMY. The order of effective leaf area index was HNUEHY> HNUEMY> MNUEHY, the order of high effective leaf area index was MNUEHY> HNUEHY> HNUEMY. From heading to maturity stage, the photosynthetic potentials of HNUEHY and MNUEHY were 10.58% and 9.86% higher than that of HNUEMY, showing no obvious difference between each other. The total dry matter accumulation of HNUEHY was slightly lower than that of MNUEHY. From heading to maturity stage, the dry matter accumulations increased with increasing yield level with the highest ratio for HNUEHY. Before jointing stage, the crop growth rate of HNUEHY was lower than that of MNUEHY. After joining stage, the crop growth rate of HNUEHY were 8.79% and 17.46% higher than that of MNUEHY and HNUEMY. 【Conclusion】The leaf area index, dry matter accumulation and crop growth rate of HNUEHY were less than those of MNUEHY and HNUEMY before jointing stage; while the photosynthetic potential, harvest index, percentage of productive tillers, crop growth rate and dry matter accumulation and ratio of HNUEHY were the highest from heading to maturity stage.

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Study on the Interaction Between Ammonium and Potassium Absorption in Rice Roots at the Seedling Stage YAN Jinxiang1, LI Fuming1,2, CHU Guang, XU Chunmei 1, CHEN Song1, ZHANG Xiufu1, WANG Danying1,* 2017, 31(4): 409-416.  DOI: 10.16819/j.1001-7216.2017.6155 Abstract ( )   HTML ( )   PDF (762KB) ( )   【Objective】 In order to clarify the interaction between NH4+ and K+ uptake in rice roots at the seedling stage, and to lay a solid basis for fertilizer application in rice production, 【Method】 we conducted an hydroponic experiment under different NH4+ and K+ concentrations，and analyzed NH4+ and K+ uptake rate of rice roots at the seedling stage. 【Result】 When K+ concentration was less than 0.2 mmol/L, the K+ uptake curve through high-affinity transport system (HATS) in rice root followed the Michaelich-Menten equation. NH4+ decreased K+ uptake rate, the Vmax of K kept decreasing with the increase of NH4+ concentration, however, the effect of NH4+ on Km was relatively little. Compared with zero NH4+ treatment, the Vmax and Km of K+ uptake at the 1.62 mmol/L NH4+ application level decreased by 47.30% and 12.33% in rice variety Qilisimiao(QL), and by 39.21% and16.46% in rice variety Huke 3(HK3), respectively. When K+ concentration was higher than 0.5 mmol/L, it was mainly absorbed through low-affinity transport system(LATS), in which the uptake rate of K+ was kept increasing with the increase of K+ concentration, and NH4+ greatly decreased the uptake rate of K+-LATS at the same concentration of K+. The uptake rate of NH4+ was increased with the increase of NH4+ concentration, however, no significant difference was observed in NH4+ uptake rate under different concentrations of K+. 【Conclusion】 NH4+ reduced K+ uptake both through HATS and LATS of K+; and the effects of NH4+ on K+-HATS uptake were mainly due to the competition of NH4+ for K+-carrier at the cell membrane; K+ had no influence on NH4+ absorption. The interactions between NH4+ and K absorption in rice root at the seedling stage were mainly because NH4+ reduced K+ uptake.

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Effects of Urease Inhibitor and Nitrification Inhibitor on Nitrogen Transformation in Paddy Soil Wenxue ZHANG, Chengchun YANG, Shaoxian WANG, Gang SUN, Zengbing LIU, Zuzhang LI, Guangrong LIU 2017, 31(4): 417-424.  DOI: 10.16819/j.1001-7216.2017.7008 417 Abstract ( )   HTML ( )   PDF (1053KB) ( )   【Objective】The research is aimed to reveal the effects of urease inhibitor(UI) and nitrification inhibitor(NI) on nitrogen (N) transformation, and the fertilizer-saving mechanism of inhibitor(s). 【Method】 A field experiment was conducted in the middle and lower reaches of the Yangtze River area, Southern China with a randomized design and five treatments and three replicates: 1) CK (no N fertilizer), 2) U (urea only), 3) U+UI, 4) U+NI, 5) U+UI+NI. NBPT [N-(n-butyl) thiophosphoric triamide] and DMPP [3, 4-Dimethyl-1H-pyrazole phosphate] as the urease inhibitor and nitrification inhibitor was uniformly mixed with urea (U) at a rate of 10,000 mg/kg. The total urea, as base fertilizer, was applied to field before the transplanting of rice seedlings. The activities of urease and nitrate reductase, the contents of NH4+-N, NO3--N and microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) in soil were analyzed in tillering and booting stages. The rice yield and N efficiency were investigated. The mechanism of increased yield and N efficiency due to inhibitor was elucidated by the stepwise regression analysis. 【Result】1) Compared with the normal urea treatment, NBPT addition and NBPT + DMPP significantly improved the grain yields and the recovery of applied N in the above-ground parts by 6.56% and 8.24%,19.4% and 23.7%, respectively. 2) The addition of NBPT and NBPT + DMPP in urea significantly reduced urease activity and soil NH4+-N content at the tillering stage, and increased soil NH4+-N content at the booting stage, without obvious effects on nitrate reductase activity (NRA), soil NO3--N content and microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) in both stages. There is no significant difference in urease activity between urea and urea + NBPT at the booting stage. Therefore, the urease inhibitor NBPT was effective on inhibiting the activity of urease and improving the content of NH4+ -N before the booting stage. On the contrast, adding DMPP only had no obvious effects on these indexes above. 3)The stepwise regression analysis revealed that the grain yield of rice was significantly associated with NH4+-N content in soil at the tillering and booting stages, especially, the latter. However, other properties in soil have no obvious effects on the grain yield. 【Conclusion】Urea combined with NBPT and combination of NBPT + DMPP slowed down the hydrolytic action of urea and dramatically improved soil NH4+-N content in the booting stage, which is the dominate factor of improving the grain yields and the recovery of applied N in the above-ground parts. The conclusion is consistent with postponing nitrogen technique in agriculture.

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Effects of Cadmium Stress on Rice Seed Germination Characteristics Yali SUN, Hongmei LIU, Qingguo XU 2017, 31(4): 425-431.  DOI: 10.16819/j.1001-7216.2017.6142 425 Abstract ( )   HTML ( )   PDF (541KB) ( )   【Objective】In order to reveal the differences in cadmium stress for different rice varieties,【Method】fifty rice varieties with different genetic background were used to analyze the effects of cadmium stress (0 mmol/L, 0.5mmol/L, 0.1 mmol/L, 0.2 mmol/L) on seed germination rate, germination index, vigor index, germ length, radicle length, germ fresh weight and germ dry weight of different rice varieties．【Result】Cd had an increased inhibition to the germination and growth of rice with the increasing cadmium concentration. 0.05 mmol/L cadmium stress had no significant impact on the germination rate and germination index, but it had a significant effect on seed vigor index and the growth of roots and shoots．The inhibitory effects of various cadmium treatments on the radicle of rice seeds were significantly greater than that on their germs. Under 0.1 mmol/L and 0.2 mmol/L cadmium stress, there are highly significant and positive correlation among indexes. Significant differences of cadmium tolerance were noted in different rice varieties.【Conclusion】According to the results of cluster analysis based on the relative value of each trait at germination stage under cadmium stress, the 50 rice varieties were divided into three types: sensitive,intermediate and tolerant．

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Loop-mediated Isothermal Amplification Assay for Rapid Diagnosis of Aphelenchoides besseyi Zongshi BAI, Meng QIN, Lirong ZHAO, Yuchun HAN, Dongwei WANG, Chunling XU, Hui XIE 2017, 31(4): 432-440.  DOI: 10.16819/j.1001-7216.2017.7001 Abstract ( )   HTML ( )   PDF (88903KB) ( )   【Objective】 This study was performed to provide a new method for the rapid detection and early diagnosis of Aphelenchoides besseyi. 【Method】The loop-mediated isothermal amplification (LAMP) specific primers of A. besseyi were designed based on the nematode 18S ribosomal RNA gene, including two external primer, two inner primer and two loop primer. The DNA sample was amplified by LAMP primers using isothermal amplification. The amplified products were detected by electrophoresis and fluorescent dye. It proved that there was A. besseyi in the test sample if there was clear DNA ladder in the electrophoresis results or it showed green fluorescence in the fluorescent dye results. 【Result】The results showed that LAMP primers could detect and identify single egg, juvenile, female and male of A. besseyi, and could also directly detect A. besseyi from multiple nematode species samples and plant tissue samples, the sensitivity of LAMP assay was 1/1000 of single nematode DNA. 【Conclusion】This diagnosis method has the advantages of accuracy, sensitivity, stability and intuitive results, and is also simple and more practical.

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Genealogical Analysis on Resistance to Magnaporthe oryzae of Super Early Rice Zhongzao 39 Yan LIANG, Zhijuan JI, Yuxiang ZENG, Cuizhen ZHANG, Hanlin WU, Changdeng YANG 2017, 31(4): 441-446.  DOI: 10.16819/j.1001-7216.2017.6165 441 Abstract ( )   HTML ( )   PDF (1007KB) ( )   【Objective】 To clarify the genetic basis of the durable resistance to Magnaporthe oryzae of the super early rice Zhongzao 39,【Method】blast resistance assessment, molecular marker screening and gene sequencing were conducted.【Result】Zhongzao 39, Jinzao 47 and Zhongzu 3 showed positive bands to all the molecular markers for 7 resistant genes Piz, Pi9, Pib, Pi36, Pi64, Pi-d2 and Pi-d3; The resistance performance of Zhongzao 39, were fully consistent with Jinzao 47 and Zhongzu 3 to the 5 isolates collected from Zhejiang and Jiangxi Province, China; Molecular sequencing results showed that all the test materials contain Pi-d2 resistant alleles. 【Conclusion】These results showed that the genetic basis of durable resistance to Magnaporthe oryzae of Zhongzao 39 was contributed by Jinzao 47 and Zhongzu 3.