Chinese Journal OF Rice Science >
Functional Study of Rice Eukaryotic Translation Initiation Factor OseIF6.2 in Grain Size Regulation
Received date: 2024-10-25
Revised date: 2025-03-27
Online published: 2025-05-21
【Objective】This study aims to investigate the functional role of the rice eukaryotic translation initiation factor OseIF6.2 in rice growth and development using a reverse genetics approach, thereby laying a foundation for further exploration of the functions of OseIF6.2.【Method】First, bioinformatics methods were employed to perform multiple sequence alignment and a phylogenetic tree was constructed to analyze the evolutionary relationship between OseIF6.2 and eIF6 proteins in other eukaryotes. CRISPR/Cas9 gene editing technology was used to precisely edit the OseIF6.2 gene in rice (Nipponbare).Next, the expression levels of OseIF6.2 in different tissues of rice were detected using quantitative real-time PCR (qRT-PCR) technology. The precise localization of OseIF6.2 within cells was observed using a confocal laser microscope.Finally, the interaction between OseIF6.2 and its potential interacting protein, OseIF6.1, was validated using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. 【Result】Phylogenetic analysis revealed that OseIF6.2 is highly conserved among eukaryotic eIF6 proteins, underscoring its universal role in translation regulation.qRT-PCR results indicated that OseIF6.2 expression was relatively high in rice stems. Subcellular localization analysis using GFP-tagged OseIF6.2 in tobacco leaf cells showed fluorescence signals in both the nucleus and cytoplasm, confirming its dual localization.Additionally, compared to the wild type, the oseif6.2 mutant exhibited significant reductions in grain length, grain thickness, and 1000-grain weight. Both Y2H and BiFC experiments confirmed a direct interaction between OseIF6.2 and OseIF6.1【Conclusion】In summary, OseIF6.2, a eukaryotic translation initiation factor in rice, plays a crucial role in regulating grain morphology, further highlighting its importance in rice growth, development, and grain formation.
MA Shunting, HU Yungao, GAO Fangyuan, LIU Liping, MOU Changling, LÜ Jianqun, SU Xiangwen, LIU Song, LIANG Yuyu, REN Guangjun, GUO Hongming . Functional Study of Rice Eukaryotic Translation Initiation Factor OseIF6.2 in Grain Size Regulation[J]. Chinese Journal OF Rice Science, 2025 , 39(3) : 331 -342 . DOI: 10.16819/j.1001-7216.2025.2401010
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