Chinese Journal OF Rice Science >
Mapping of qChalk8 Controlling Chalky Rice Rate in japonica Rice by Combining QTL-Seq with Molecular Markers
Received date: 2023-12-13
Revised date: 2024-03-18
Online published: 2024-11-15
【Objective】Chalkiness is an important characteristic that affects the appearance quality of rice grains. This study aims to use QTL-Seq and molecular marker mapping methods to locate QTLs related to chalky rice rates in japonica rice.【Method】F2 segregating populations were constructed using the japonica germplasm Lamujia with a high chalky rice rate and the japonica variety Shuijing 3 with a low chalky rice rate. Two independent F2 populations were planted in Yuanyang, Henan Province, and Sanya, Hainan Province, respectively. The single-plant chalky rice rate was examined, and extreme individuals were pooled for QTL-Seq analysis. Reliable QTLs were then subjected to verification using molecular marker mapping.【Result】QTL-Seq analysis of the two F2 populations revealed the existence of a stable chalky rice rate QTL on chromosome 8, designated as qChalk8. Further molecular marker mapping confined the QTL to a genomic region between 17.6 Mb and 18.5 Mb. The LOD score for qChalk8 was 7.08, and qChalk8 could explain 15.9% of the phenotypic variation.【Conclusion】QTL-Seq and molecular marker mapping successfully identified qChalk8 related to chalky rice rates in japonica rice. The localization of qChalk8 establishes a foundation for further fine mapping and gene cloning of the chalkiness regulatory gene in japonica rice.
Key words： rice; grain chalkiness; chalky rice rate; QTL-Seq; QTL mapping
DU Yanxiu, SUN Wenyu, YUAN Zeke, ZHANG Qianqian, LI Fuhao, LI Junzhou, SUN Hongzheng . Mapping of qChalk8 Controlling Chalky Rice Rate in japonica Rice by Combining QTL-Seq with Molecular Markers[J]. Chinese Journal OF Rice Science, 2024 , 38(6) : 665 -671 . DOI: 10.16819/j.1001-7216.2024.231207
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