Chinese Journal OF Rice Science >
Map-based Cloning and Functional Analysis of a New Allele of D1, a Gene Controlling Plant Height in Rice (Oryza sativa L.)
Received date: 2023-05-06
Revised date: 2023-06-18
Online published: 2024-03-14
【Objective】Plant height is an important agronomic trait in rice breeding. Identification of genes controlling plant height and their functional characterization can provide useful genetic resources for high-yield breeding.【Methods】A dwarf mutant, d1-11, was screened from the rice variety Nipponbare by EMS mutagenesis. Phenotypic and cytological observations, gene expression, hormone content and drought resistance were analyzed. The d1-11 mutant gene was identified through map-based cloning.【Results】The d1-11 mutant exhibits dwarfism, with more shortened and widened leaves, and more rounded grains compared to the wild type. The d1-11 mutant leaf has a smaller midvein, reduced number and area of large and small veins, resulting in abnormal leaf morphology in the d1-11 mutant. The d1-11 gene is genetically mapped between two molecular markers on rice chromosome 5. Map-based cloning reveals that a single base substitution at the junction of the ninth exon-intron in the D1 gene results in the loss-of-function mutation of d1-11. The D1 gene has a higher expression level in various tissues at the seedling stage, but the expression levels decrease from the tillering stage. Exogenous abscisic acid (ABA) treatment for 24 hours induces D1 gene expression, exogenous gibberellin (GA) treatment inhibits D1 gene expression, and salt stress treatment for 24 hours can strongly induce D1 gene expression. The contents of several hormones such as GA, brassinosteroid (BR), and indole-3-acetic acid (IAA) were increased in the d1-11 mutant. The d1-11 mutant shows a significant increase in relative water content (RWC) and a reduced rate of water loss in leaves. Furthermore, d1-11 mutant plants exhibit stronger resistance to drought stress.【Conclusion】The d1-11, a novel allele in D1 locus was identified in the present study. It was showed that the d1-11 mutant had increased levels of various endogenous hormones, increased leaf water content, and enhanced resistance to drought stress. This study will further enrich the genetic resources related to dwarfism and reveals some new biological roles of the D1 gene in rice.
Key words： plant height; dwarfism; D1 gene; d1-11 mutant; plant hormones; drought stress
GAO Junru, QUAN Hongyu, YUAN Liuzhen, LI Qinying, QIAO Lei, LI Wenqiang . Map-based Cloning and Functional Analysis of a New Allele of D1, a Gene Controlling Plant Height in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2024 , 38(2) : 140 -149 . DOI: 10.16819/j.1001-7216.2024.230502
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