Chinese Journal OF Rice Science >
Seedling Growth Characteristics of Hybrids Between Transgenic Rice with cry2A*/bar Genes and Weedy Rice
Received date: 2022-06-12
Revised date: 2022-10-20
Online published: 2023-07-17
【Objectives】 The study aims to evaluate the seedling growth characteristics of hybrids between transgenic rice T2A-1 with cry2A*/bar gene and weedy rice, and lay an experimental basis for evaluating the potential ecological risks of gene flow from T2A-1 to weedy rice. 【Methods】 F1+-F4+ (positive) hybrids (those with transgenic insertion using the transgenic line T2A-1 as the paternal parent), F1− -F4−(negative) hybrids(those using the non-transgenic receptor Minghui 63 (MH63) as the paternal parent) with three weedy rice (WRMM, WRTZ and WRYY) accessions, were used as experimental materials. Under hydroponic culture, germinability was measured. Eight root development indexes were measured and relative root development index under normal nitrogen (40 mg/L) and low nitrogen (20 mg/L), and the seedling growth characteristics of hybrids were evaluated. 【Results】 Transgenic positive hybrids had similar 个germinability to transgenic negative hybrids, but both showed weeker germination potential and germination index compared with the corresponding weedy rice. There were no significant differences in plant height, whole plant dry biomass, and root development between T2A-1 and MH63, and between transgenic positive and transgenic negative hybrids under normal nitrogen conditions. Compared with the weedy rice, hybrids derived from WRMM displayed significantly lower plant height, but similar whole plant dry biomass and the index of root development. Hybrids of WRYY displayed similar seedling growth characteristics with WRYY. While hybrids derived from WRTZ had significantly greater plant height, but significantly lower whole plant dry biomass and index of root development. Under low nitrogen conditions, transgenic positive hybrids had similar or significantly lower plant height, whole plant dry biomass and index of root development than transgene negative hybrids. The relative root development indexes of all hybrids were significantly lower compared with their corresponding weedy rice. 【Conclution】 Compared with their corresponding weedy rice, transgenic positive hybrids displayed equivalent or lower seedling growth momentum under normal nitrogen conditions, but significantly weaker growth under low nitrogen conditions.
XIAO Lequan, LI Lei, DAI Weimin, QIANG Sheng, SONG Xiaoling . Seedling Growth Characteristics of Hybrids Between Transgenic Rice with cry2A*/bar Genes and Weedy Rice[J]. Chinese Journal OF Rice Science, 2023 , 37(4) : 347 -358 . DOI: 10.16819/j.1001-7216.2023.220612
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